FastQCFastQC Report
Fri 10 Feb 2017
SRR2031748_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031748_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325769
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.7308859959050739No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23480.7207561185993756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20660.6341917125325001No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.4171667654073899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.32139337997169776No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC6150.18878407706073935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.16729645853350072No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA4690.1439670441324988No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG4480.13752075857432722No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4470.13721379259536667No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT4150.127390881268629No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT3840.11787493592085188No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3740.11480527613124637No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC3730.11449831015228583No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC3630.11142865036268029No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT3370.10344753490970596No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA3310.10160573903594265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.053.5580861
GCGTCAG451.5133728E-552.77881
CGTCAGA608.276701E-539.58412
GTATCAA24950.038.457661
CGGAGAT500.001613960338.0007365
GGGATCC756.913375E-637.99497
TCAACGC29850.031.9855444
ATCAACG30050.031.9307353
TATCAAC30100.031.8776932
GGAGTCT902.4099001E-531.6624156
CAACGCA30750.031.2038575
AACGCAG31150.030.6459666
GTGGTAT6950.028.7055931
ACGCAGA34550.027.6301567
CGCAGAG34450.027.580978
GAGTACT18200.026.7580612-13
GCAGAGT35950.025.6372599
GTATTAT950.001224526825.0004861
TCGGAGA950.001224526825.0004864
GTACTTT19500.024.97610914-15
Produced by FastQC (version 0.11.5)