##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031748_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 325769 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76225791895484 33.0 31.0 34.0 30.0 34.0 2 32.064217282798545 34.0 31.0 34.0 31.0 34.0 3 32.14409903950345 34.0 31.0 34.0 31.0 34.0 4 35.54416473022295 37.0 35.0 37.0 35.0 37.0 5 35.548112312712384 37.0 37.0 37.0 35.0 37.0 6 35.598657330808024 37.0 37.0 37.0 35.0 37.0 7 35.584414109384255 37.0 37.0 37.0 35.0 37.0 8 35.588312577317055 37.0 37.0 37.0 35.0 37.0 9 37.336569777971505 39.0 39.0 39.0 35.0 39.0 10-11 37.276867964723465 39.0 38.0 39.0 35.0 39.0 12-13 37.236264039856465 39.0 38.0 39.0 35.0 39.0 14-15 38.54386236873367 41.0 39.0 41.0 35.5 41.0 16-17 38.45712606171858 41.0 39.0 41.0 35.5 41.0 18-19 38.48847649714982 41.0 39.0 41.0 35.0 41.0 20-21 38.51140225128849 41.0 39.0 41.0 35.0 41.0 22-23 38.39388800039292 41.0 39.0 41.0 35.0 41.0 24-25 38.31611356513358 41.0 39.0 41.0 35.0 41.0 26-27 38.20274335495397 41.0 38.5 41.0 35.0 41.0 28-29 38.07366569563095 40.0 38.0 41.0 34.0 41.0 30-31 37.86602162882288 40.0 38.0 41.0 34.0 41.0 32-33 37.59649629031614 40.0 38.0 41.0 34.0 41.0 34-35 37.434849540625414 40.0 38.0 41.0 33.5 41.0 36-37 37.25611552971584 40.0 37.0 41.0 33.0 41.0 38-39 37.05004312872005 40.0 37.0 41.0 33.0 41.0 40-41 36.941854504265294 40.0 37.0 41.0 33.0 41.0 42-43 36.82696327765994 40.0 37.0 41.0 31.5 41.0 44-45 36.57507159981459 40.0 37.0 41.0 31.0 41.0 46-47 36.317039067560145 40.0 37.0 41.0 30.0 41.0 48-49 36.1642267987439 40.0 37.0 41.0 28.5 41.0 50-51 35.59982226669818 39.5 36.5 40.5 27.0 41.0 52-53 35.6096804791125 39.5 37.0 40.5 26.0 41.0 54-55 35.83351546648085 40.0 37.0 41.0 26.0 41.0 56-57 35.72989296096314 40.0 37.0 41.0 25.5 41.0 58-59 35.590627100798415 40.0 36.0 41.0 25.0 41.0 60-61 35.40698623871516 40.0 36.0 41.0 23.5 41.0 62-63 35.153542847846175 39.5 35.5 41.0 22.5 41.0 64-65 34.916641853583364 39.0 35.0 41.0 21.5 41.0 66-67 34.65519432481298 39.0 35.0 41.0 19.5 41.0 68-69 34.32424509391624 38.5 35.0 41.0 17.5 41.0 70-71 33.97709880313965 37.5 35.0 40.0 13.5 41.0 72-73 33.595667482172956 37.0 35.0 39.5 7.5 41.0 74-75 33.156692318790306 37.0 35.0 39.0 4.5 41.0 76-77 32.75531588334064 36.0 34.0 39.0 2.0 41.0 78-79 32.317715927543745 36.0 34.0 38.0 2.0 39.5 80-81 31.897919998526564 35.0 34.0 37.0 2.0 39.0 82-83 31.495759265000657 35.0 34.0 37.0 2.0 39.0 84-85 31.110199251616944 35.0 34.0 36.0 2.0 38.0 86-87 30.774462886278314 35.0 33.0 36.0 2.0 37.0 88-89 30.43939263711403 35.0 33.0 36.0 2.0 37.0 90-91 30.155757607384373 35.0 33.0 35.0 2.0 36.0 92-93 29.90184302373768 35.0 33.0 35.0 2.0 36.0 94-95 29.62097222264856 35.0 32.0 35.0 2.0 36.0 96-97 29.347137081797225 35.0 32.0 35.0 2.0 36.0 98-99 29.10673053605469 35.0 32.0 35.0 2.0 35.5 100-101 28.56602070792494 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05622029201817824 1101 2 0.027331058513581752 1101 3 0.06148089311700744 1101 4 0.04122797207210738 1101 5 0.042857234843026504 1101 6 0.01229973131891171 1101 7 0.03449764822966017 1101 8 -0.14202100937259132 1101 9 0.042698198870411375 1101 10-11 0.06558079456672061 1101 12-13 0.09142194420601868 1101 14-15 -0.033421333750339954 1101 16-17 0.07784135342969734 1101 18-19 0.11665964285636932 1101 20-21 -0.011991137358954518 1101 22-23 0.05828362494943207 1101 24-25 0.1911255266691967 1101 26-27 -0.11510382705990452 1101 28-29 -0.13419372884140301 1101 30-31 -0.15789012238531086 1101 32-33 0.14637523688281107 1101 34-35 0.406333066773108 1101 36-37 -0.01036373259835699 1101 38-39 0.009009768763654336 1101 40-41 -0.6316052069360651 1101 42-43 -0.6711888216063571 1101 44-45 -0.4673704959115099 1101 46-47 -0.13710295233521208 1101 48-49 -0.006675189690497518 1101 50-51 0.099367192345305 1101 52-53 0.021489041446372426 1101 54-55 0.0075171975455674556 1101 56-57 -0.08408559652864511 1101 58-59 -0.0938217342520602 1101 60-61 -0.10920392270831769 1101 62-63 -0.23362994004164506 1101 64-65 -0.012722780609983886 1101 66-67 -0.45134387975887336 1101 68-69 -0.2852514714531864 1101 70-71 -0.08894591579888811 1101 72-73 0.27314332918557227 1101 74-75 0.263858599651293 1101 76-77 0.497460398243355 1101 78-79 0.3111488837411045 1101 80-81 0.3945609245857895 1101 82-83 0.20093490278937765 1101 84-85 0.1686610456481894 1101 86-87 0.06426577522823962 1101 88-89 -0.2466967817131831 1101 90-91 -0.6333460496020642 1101 92-93 -0.640622903192277 1101 94-95 -0.41182831730417746 1101 96-97 -0.6245510907835481 1101 98-99 -0.41284838703199256 1101 100-101 -0.515200386916117 1102 1 0.07396845492763049 1102 2 0.10207573406471226 1102 3 -0.06899924139538882 1102 4 -0.016204460360327744 1102 5 -0.0031634402099101067 1102 6 -0.08511277335165346 1102 7 -0.16347221103052334 1102 8 -0.16240025959054805 1102 9 -0.04512407040346744 1102 10-11 -0.08650850671612886 1102 12-13 -0.02832366545894871 1102 14-15 -0.032557278450802585 1102 16-17 0.14181258272350306 1102 18-19 0.16738202422390458 1102 20-21 0.04519998233216427 1102 22-23 0.39646397119430077 1102 24-25 -0.033194127085941716 1102 26-27 0.1034398894676869 1102 28-29 -0.05118214582982006 1102 30-31 -0.15928350885933895 1102 32-33 0.1866433275541226 1102 34-35 0.25664910862141 1102 36-37 0.2613077084279283 1102 38-39 0.20464434343048055 1102 40-41 0.6679106107249098 1102 42-43 0.7769600761393249 1102 44-45 0.38150545377089173 1102 46-47 0.781963867941279 1102 48-49 0.7490696104091441 1102 50-51 0.7235420584556493 1102 52-53 0.853979077565441 1102 54-55 1.074859020935783 1102 56-57 1.1016006657705262 1102 58-59 1.0266867350467308 1102 60-61 1.0798840708151616 1102 62-63 1.0585141625863272 1102 64-65 1.260715692425272 1102 66-67 1.3587118557000863 1102 68-69 1.0821899907463362 1102 70-71 0.9363997063209268 1102 72-73 1.0523266023204556 1102 74-75 0.7549300282227236 1102 76-77 1.162821900298404 1102 78-79 0.8469410122751739 1102 80-81 0.9220861456593923 1102 82-83 0.9269515300156783 1102 84-85 1.050884859323613 1102 86-87 0.5287772336913115 1102 88-89 0.4802228222225473 1102 90-91 0.5579059743112502 1102 92-93 0.7855276136840494 1102 94-95 0.7117860805037708 1102 96-97 0.9197414736703067 1102 98-99 1.1195575788417145 1102 100-101 1.2260345115768452 1103 1 0.007833195243982516 1103 2 0.12186510869279488 1103 3 0.07985006874424272 1103 4 -0.11065374835799702 1103 5 -0.12022520243296242 1103 6 -0.006965501655997741 1103 7 -0.16442708295313935 1103 8 0.041222718225981225 1103 9 0.05569103041163004 1103 10-11 0.026602299943064622 1103 12-13 -0.06740422066853569 1103 14-15 0.0662650461779748 1103 16-17 0.009292966332921537 1103 18-19 -0.10825075140886753 1103 20-21 -0.09062016961701858 1103 22-23 -0.02325759368855529 1103 24-25 0.17970079548640427 1103 26-27 0.2059087177429646 1103 28-29 0.05039408477866658 1103 30-31 -0.05417148439247654 1103 32-33 -0.04381293516020435 1103 34-35 0.09293700942543381 1103 36-37 0.11418823872781303 1103 38-39 0.2840993744984175 1103 40-41 0.25526755292056436 1103 42-43 0.3953434364581625 1103 44-45 -0.17211959985416314 1103 46-47 0.20362285411639647 1103 48-49 -8.508169306367108E-4 1103 50-51 0.00976145757827851 1103 52-53 -0.3416381986969981 1103 54-55 -0.34351326840425145 1103 56-57 -0.33632573989782344 1103 58-59 -0.10031815002266598 1103 60-61 0.22614553965727424 1103 62-63 0.25696145780781876 1103 64-65 0.02782381437209125 1103 66-67 -0.09538509839686782 1103 68-69 0.03934530274035808 1103 70-71 0.11535515946992803 1103 72-73 -0.009338749667477941 1103 74-75 0.034243904310791606 1103 76-77 -0.17435859817241806 1103 78-79 0.04098759341852798 1103 80-81 0.05943547655710901 1103 82-83 0.00581841533417915 1103 84-85 -0.028023542165431792 1103 86-87 -0.1895424684993614 1103 88-89 -0.24020036594257732 1103 90-91 0.03757688946603821 1103 92-93 -0.4840368816868974 1103 94-95 -0.02114731371995049 1103 96-97 -0.0013432054788857783 1103 98-99 0.05332544809345663 1103 100-101 0.3705523012559233 1104 1 0.3613815823407549 1104 2 0.5654102373992131 1104 3 0.43653954414340035 1104 4 0.37986433570846856 1104 5 0.588370431323959 1104 6 0.6604962122573284 1104 7 0.6786618711035572 1104 8 0.4835948264045342 1104 9 0.5312465859671889 1104 10-11 0.7045690643614435 1104 12-13 0.5610480439127628 1104 14-15 0.8305596046660852 1104 16-17 0.6295275704384977 1104 18-19 0.9363957132375944 1104 20-21 0.8109634080955956 1104 22-23 0.9790754138057451 1104 24-25 1.0166900427982242 1104 26-27 0.8069709529987463 1104 28-29 0.9767153620676865 1104 30-31 1.1547565051806785 1104 32-33 0.9363239172347093 1104 34-35 0.9583198702921649 1104 36-37 0.7662638333840519 1104 38-39 0.8571329359190756 1104 40-41 0.8679035907178942 1104 42-43 0.9606806798599266 1104 44-45 0.9979814102468509 1104 46-47 0.8736521796295946 1104 48-49 1.0494978308373675 1104 50-51 0.9267572216708189 1104 52-53 1.0392089827953441 1104 54-55 0.9149292210059841 1104 56-57 0.878890943060803 1104 58-59 0.8199685883285852 1104 60-61 0.692049742980835 1104 62-63 0.7177630218351894 1104 64-65 0.6964194481290278 1104 66-67 0.7526957096839411 1104 68-69 0.8426884112447581 1104 70-71 0.7696464595676815 1104 72-73 0.8248222148160735 1104 74-75 0.971813145105834 1104 76-77 0.8346084920849961 1104 78-79 0.7975271828613373 1104 80-81 1.0553820389552868 1104 82-83 0.9662354892996419 1104 84-85 1.0773853858241402 1104 86-87 0.93049011540419 1104 88-89 1.1035011655008944 1104 90-91 0.9624970589023398 1104 92-93 0.6787392668957004 1104 94-95 0.8969106856283737 1104 96-97 0.601973835902669 1104 98-99 0.6865284458125807 1104 100-101 0.8666031321454675 1105 1 -0.2723026281855567 1105 2 -0.16045898110158419 1105 3 -0.1820250491580353 1105 4 -0.1381579928401706 1105 5 0.013824976778508358 1105 6 -0.009870613898968372 1105 7 -0.2338102182106354 1105 8 0.10094729849384976 1105 9 -0.38173587017316635 1105 10-11 -0.11080573627651802 1105 12-13 -0.10156758607129035 1105 14-15 0.03110186941887605 1105 16-17 0.12649726740819034 1105 18-19 0.19467322519932395 1105 20-21 0.3711228977287604 1105 22-23 0.09090954458724099 1105 24-25 0.2331812708684069 1105 26-27 0.026301096539420143 1105 28-29 -0.27454460603438946 1105 30-31 -0.06709357456414722 1105 32-33 0.1480942521629416 1105 34-35 -0.03064344709220279 1105 36-37 0.23969286049728566 1105 38-39 0.15997185138957093 1105 40-41 0.2764522350560057 1105 42-43 -0.13431134565842484 1105 44-45 -0.17270439517579916 1105 46-47 -0.027559941582531167 1105 48-49 -0.3982852632615206 1105 50-51 -0.37097802553811476 1105 52-53 -0.07619771576924705 1105 54-55 -0.29652213465590194 1105 56-57 -0.18729725470634406 1105 58-59 -0.33932965728544673 1105 60-61 -0.6139789651531444 1105 62-63 -0.3503390515300424 1105 64-65 -0.2591786379953831 1105 66-67 -0.5404143381629538 1105 68-69 -0.501458319217754 1105 70-71 -0.42024189768909537 1105 72-73 -0.7289576894901515 1105 74-75 -0.3999571898223948 1105 76-77 -0.33821447442218044 1105 78-79 -0.5215477772662496 1105 80-81 -0.4645222672648117 1105 82-83 -0.6018027882123249 1105 84-85 -0.31479962215034263 1105 86-87 -0.49923875221941216 1105 88-89 -0.39468065268092545 1105 90-91 -0.48621107506895456 1105 92-93 -0.33016025463539833 1105 94-95 -0.22886283909252114 1105 96-97 -0.5543898004609638 1105 98-99 -0.4061350310614209 1105 100-101 -0.17460898906665534 1106 1 -0.04167397321479882 1106 2 0.0274430656820428 1106 3 -0.046718031614176425 1106 4 0.09678352762766451 1106 5 0.024936087889621206 1106 6 0.04948611124722646 1106 7 0.04681843676012676 1106 8 0.027445836505549437 1106 9 0.05569103041163004 1106 10-11 0.24972057951295312 1106 12-13 0.16747481158953548 1106 14-15 -0.09183307210159342 1106 16-17 0.12163615629708602 1106 18-19 -0.0891535291866461 1106 20-21 0.08696719597438118 1106 22-23 0.1548898077451426 1106 24-25 0.2842594872426716 1106 26-27 0.35683837724117495 1106 28-29 0.2928896044919256 1106 30-31 0.32855701023117945 1106 32-33 0.3408756849114809 1106 34-35 0.43119865817094905 1106 36-37 0.23017166166687986 1106 38-39 0.5304031379392811 1106 40-41 0.6330677321320337 1106 42-43 0.5839635081427517 1106 44-45 0.5900331779236225 1106 46-47 0.6269539473063688 1106 48-49 0.6244852045747393 1106 50-51 0.544427676216273 1106 52-53 0.6556736292599936 1106 54-55 0.964954473531229 1106 56-57 0.8102356400304913 1106 58-59 0.7063574772174732 1106 60-61 0.6743982278293217 1106 62-63 0.7383122642594273 1106 64-65 0.6868739935835677 1106 66-67 0.6424621238253536 1106 68-69 0.6445760375073846 1106 70-71 0.6015782777494962 1106 72-73 0.6438310531999107 1106 74-75 0.6267058218735144 1106 76-77 0.6431817244082296 1106 78-79 0.6052797081138621 1106 80-81 0.39366486723811533 1106 82-83 0.4858251357642871 1106 84-85 0.4143487696625243 1106 86-87 0.24050905479812812 1106 88-89 0.30740268065240883 1106 90-91 0.3920235740538516 1106 92-93 0.5126029032593387 1106 94-95 0.44569856441625433 1106 96-97 0.6282490884279213 1106 98-99 0.64214713268127 1106 100-101 0.7045576776000111 1107 1 0.5013815823407555 1107 2 0.5815857859885512 1107 3 0.6660693247076637 1107 4 0.6024348686238312 1107 5 0.6414111836750607 1107 6 0.6515933909407181 1107 7 0.5352762911662552 1107 8 0.5988299361224065 1107 9 0.6284879652775288 1107 10-11 0.5750079358347975 1107 12-13 0.5644336238500713 1107 14-15 0.8649796673620074 1107 16-17 0.8310949685576219 1107 18-19 0.6110979075949672 1107 20-21 0.7151640350548405 1107 22-23 0.9991067617681253 1107 24-25 0.9401383186602956 1107 26-27 0.9889145266664627 1107 28-29 1.077875236675844 1107 30-31 0.921684404867193 1107 32-33 1.039019841999604 1107 34-35 1.1280377386307237 1107 36-37 0.8814048992147718 1107 38-39 1.110455819931616 1107 40-41 0.6960227129749441 1107 42-43 1.043063125000991 1107 44-45 1.2748152660462253 1107 46-47 1.3442791388772477 1107 48-49 1.579058959364005 1107 50-51 1.749923365871446 1107 52-53 1.86908359094582 1107 54-55 1.985117308780275 1107 56-57 1.9028721342833705 1107 58-59 1.8713478986734131 1107 60-61 1.9185074232316168 1107 62-63 1.7871987585123108 1107 64-65 2.1308395108249485 1107 66-67 2.1749214150130953 1107 68-69 2.182782455131907 1107 70-71 2.2905868984391518 1107 72-73 2.000715631743965 1107 74-75 2.0573930824099094 1107 76-77 1.8884642914580354 1107 78-79 1.9556149571560084 1107 80-81 1.919143794441183 1107 82-83 1.832316994001829 1107 84-85 1.6708023137238293 1107 86-87 1.5573553191659464 1107 88-89 1.8191124507673493 1107 90-91 2.1277635165825863 1107 92-93 1.9613097998110618 1107 94-95 1.9658135069449898 1107 96-97 2.0766446822976548 1107 98-99 1.8232055618000444 1107 100-101 2.07998871208277 1108 1 -0.1961719344788122 1108 2 -0.18914077733528956 1108 3 -0.2475972365071435 1108 4 -0.2504386945945569 1108 5 -0.24366737796155746 1108 6 -0.2810439601085335 1108 7 -0.229239157531417 1108 8 -0.15396981079312866 1108 9 -0.15939561586767326 1108 10-11 -0.2739797262974406 1108 12-13 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98-99 -0.44264702987452154 2316 100-101 -0.5032911596092937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4702.0 3 515.0 4 377.0 5 338.0 6 395.0 7 455.0 8 635.0 9 653.0 10 568.0 11 746.0 12 938.0 13 1222.0 14 1446.0 15 1914.0 16 2658.0 17 3857.0 18 3589.0 19 1800.0 20 1472.0 21 1282.0 22 1240.0 23 1271.0 24 1347.0 25 1529.0 26 1724.0 27 1976.0 28 2374.0 29 2973.0 30 3621.0 31 4287.0 32 5584.0 33 7373.0 34 10591.0 35 17916.0 36 25217.0 37 53683.0 38 108924.0 39 44550.0 40 27.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.92717715409917 24.450831514555347 16.758783929175248 19.86320740217024 2 14.942207193359975 24.54288349216108 41.599446664617275 18.915462649861666 3 16.248874188878062 31.17935823386747 34.34915053132137 18.22261704593309 4 11.835678254481069 22.266248197889418 41.62821651763071 24.2698570299988 5 11.01606612098616 39.926784962332064 36.25158678686801 12.805562129813767 6 23.111436175116623 41.35879485934495 21.87390521379446 13.655863751743974 7 20.717431457697543 37.466308934394235 26.855121502967272 14.961138104940947 8 21.772372064151536 35.66531557970235 24.626863378372978 17.935448977773135 9 24.782206543483404 18.912615273044622 25.005915377831318 31.29926280564066 10-11 20.69713528984316 29.844174638704235 31.289511323186687 18.169178748265917 12-13 22.553026717721465 29.704642257884096 30.440808599824216 17.301522424570223 14-15 20.920100201324747 30.65130861085924 29.569072830380055 18.859518357435952 16-17 17.63552219214802 33.39158608881163 30.541197301621704 18.43169441741865 18-19 17.27336442134191 31.384389803051217 34.00110047861598 17.3411452969909 20-21 19.74746263885559 30.063503187454277 33.513914759419436 16.675119414270696 22-23 19.52101053408017 30.315561395016484 32.90075221476923 17.262675856134123 24-25 18.588264216462054 30.890862437195583 33.23110489682406 17.28976844951831 26-27 18.115927554113917 30.573921755124054 33.812997174260104 17.49715351650192 28-29 17.839932634270472 30.4259837486324 34.24788252793588 17.486201089161245 30-31 18.64796886499509 30.36400483680396 33.23965994871267 17.74836634948828 32-33 17.872403191533497 30.84181074814818 33.65045855631201 17.63532750400632 34-35 18.14226947019674 31.198569037926948 33.15876579521199 17.500395696664313 36-37 18.492718077332682 30.28917154971178 32.96405064536107 18.25405972759447 38-39 17.87291973012383 31.21845311354805 33.368519875786895 17.54010728054123 40-41 17.780676764976135 30.589488047055784 33.64663906994754 17.983196118020533 42-43 18.003893447975454 30.509789701568902 33.114896663834415 18.37142018662123 44-45 18.559834766880225 30.595481599527908 32.76478100310888 18.07990263048299 46-47 18.5158605513159 30.706555981399465 32.15399840795163 18.623585059333 48-49 18.14983647272906 30.99640977720946 32.10575173363498 18.748002016426497 50-51 18.13837054020786 30.99806217930297 31.6315036474365 19.23206363305267 52-53 18.404244367654673 31.247820121412516 31.923102334064186 18.424833176868628 54-55 18.958868499649707 31.05726330797311 31.072012389228256 18.911855803148928 56-57 18.619442318744372 31.28482434546365 31.129468964266728 18.96626437152525 58-59 18.04592261621243 30.920480491185153 31.75725300153342 19.276343891068997 60-61 18.757365628654004 31.316002562893058 31.292186710139564 18.634445098313375 62-63 18.57636514772743 31.129317007438228 31.319382877477427 18.974934967356916 64-65 18.67017892216379 30.97972256460224 31.130335621217387 19.219762892016586 66-67 18.618152537372147 30.898714103068446 30.91884465971676 19.564288699842646 68-69 18.461401333007537 31.058276433859067 31.093929851900004 19.386392381233396 70-71 18.70899240005286 30.96432972645722 31.389349010593214 18.937328862896706 72-73 18.870200433935473 30.465374284713302 31.308966975420567 19.355458305930657 74-75 18.43639190526232 30.863338311117666 30.72168654707693 19.97858323654308 76-77 18.125642558462253 31.310750108725887 31.16783127586562 19.39577605694624 78-79 18.602220931340458 30.982852787228875 30.751723480831945 19.66320280059872 80-81 18.708036544416526 30.840536947818936 30.704686383439757 19.746740124324784 82-83 18.583317324317054 31.60364237138356 30.526606985053984 19.2864333192454 84-85 18.641663798190404 30.700328235641226 30.49195392407553 20.16605404209284 86-87 17.996575974033956 31.03768887468188 31.24623480089012 19.71950035039404 88-89 19.025374439320622 30.92583742718275 30.14891521313884 19.89987292035779 90-91 18.52282139822076 30.99195207821406 30.83458962053211 19.65063690303307 92-93 19.020612297907725 31.01635181846546 30.404945353049577 19.55809053057724 94-95 18.851627734018457 30.427700827619695 30.687234742202456 20.03343669615939 96-97 19.251356616539965 30.04882590446224 30.634951850099252 20.06486562889854 98-99 18.879369683858595 30.7523415605487 30.136836865135948 20.231451890456757 100-101 18.981134917694874 30.713362343924604 30.22609508321203 20.07940765516849 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3069.0 1 1755.0 2 433.0 3 395.5 4 301.0 5 266.5 6 371.5 7 618.0 8 912.0 9 951.0 10 680.5 11 473.5 12 454.5 13 466.0 14 495.5 15 516.5 16 541.5 17 575.0 18 611.0 19 662.0 20 745.5 21 824.5 22 889.0 23 998.5 24 1122.0 25 1314.0 26 1734.5 27 2436.5 28 3312.5 29 4199.5 30 5678.5 31 8034.5 32 11604.5 33 15664.5 34 19623.0 35 22214.5 36 23754.5 37 24935.5 38 22570.0 39 18924.5 40 16091.5 41 13337.5 42 11213.5 43 9453.0 44 8184.0 45 7391.5 46 6415.5 47 5514.5 48 5254.0 49 4977.5 50 4557.5 51 4177.5 52 3784.5 53 3328.5 54 2855.5 55 2529.0 56 2251.0 57 1910.0 58 1666.5 59 1482.5 60 1229.5 61 1025.5 62 811.5 63 596.0 64 464.5 65 350.0 66 257.5 67 182.5 68 136.0 69 128.5 70 128.0 71 102.0 72 73.0 73 60.5 74 57.5 75 49.0 76 33.5 77 20.5 78 16.0 79 15.5 80 15.0 81 12.0 82 13.5 83 9.5 84 3.5 85 3.0 86 1.0 87 2.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14089738434289328 2 0.1439670441324988 3 0.13752075857432722 4 0.13997648640601162 5 0.13076750703719509 6 0.11142865036268029 7 0.11848886787877301 8 0.10805202459411424 9 0.10621022872035094 10-11 0.09469900450933023 12-13 0.11265651427852252 14-15 0.13015357507927394 16-17 0.14197176526925523 18-19 0.13997648640601162 20-21 0.13230233693199783 22-23 0.12171201065785879 24-25 0.10866595655203534 26-27 0.1128099972680028 28-29 0.11695403798397024 30-31 0.10667067768879174 32-33 0.10605674573087064 34-35 0.12079111272097712 36-37 0.12539560240538541 38-39 0.11066123541527892 40-41 0.11296348025748307 42-43 0.10759157562567341 44-45 0.1260095343633065 46-47 0.12340032354214182 48-49 0.13537199672160333 50-51 0.12386077251058265 52-53 0.10728460964671285 54-55 0.09976394316217935 56-57 0.11848886787877301 58-59 0.10805202459411424 60-61 0.10912640552047617 62-63 0.10912640552047617 64-65 0.13260930291095838 66-67 0.12063762973149686 68-69 0.1272373982791487 70-71 0.11419134417332527 72-73 0.11511224211020693 74-75 0.09915001120425822 76-77 0.12570256838434596 78-79 0.12585605137382624 80-81 0.12631650034226707 82-83 0.1324558199214781 84-85 0.12063762973149686 86-87 0.1292326771423923 88-89 0.11741448695241107 90-91 0.12447470446850376 92-93 0.13920907145861025 94-95 0.11603314004708858 96-97 0.10037787512010045 98-99 0.10590326274139038 100-101 0.1490319827853479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 325769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.91593865915873 #Duplication Level Percentage of deduplicated Percentage of total 1 70.40009494898324 28.10085871582566 2 13.953419897606423 11.139277054366858 3 5.572050211650024 6.6724084336192435 4 2.7978742191710326 4.467191028338903 5 1.5860017615595954 3.1653374513865256 6 1.064962555582891 2.550538802576856 7 0.6969818816032441 1.947448022283409 8 0.5263158187349561 1.6806711948775537 9 0.4190251849938746 1.5053205222772326 >10 2.605927580983015 20.164151525724574 >50 0.24693394789804668 6.794585824883871 >100 0.12501031112338612 8.620829052398102 >500 0.0015433371743627919 0.3573017614971345 >1k 0.0038583429359069794 2.8340806099440816 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2381 0.7308859959050739 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2348 0.7207561185993756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2066 0.6341917125325001 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.4171667654073899 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.32139337997169776 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 615 0.18878407706073935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.16729645853350072 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 469 0.1439670441324988 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 448 0.13752075857432722 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 447 0.13721379259536667 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 415 0.127390881268629 No Hit TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT 384 0.11787493592085188 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 374 0.11480527613124637 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 373 0.11449831015228583 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 363 0.11142865036268029 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 337 0.10344753490970596 No Hit AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA 331 0.10160573903594265 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.069659789605518E-4 0.0 16-17 0.0 0.0 0.0 6.139319579211036E-4 0.0 18-19 0.0 0.0 0.0 9.208979368816554E-4 0.0 20-21 0.0 0.0 0.0 0.0012278639158422072 0.0 22-23 0.0 0.0 0.0 0.0021487618527238628 0.0 24-25 0.0 0.0 0.0 0.004911455663368829 0.0 26-27 0.0 0.0 0.0 0.01089729225309959 0.0 28-29 0.0 0.0 0.0 0.02363638037996249 0.0 30-31 0.0 0.0 0.0 0.05233769941277408 0.0 32-33 0.0 0.0 0.0 0.1019127050149032 0.0 34-35 0.0 0.0 0.0 0.1846400363447719 0.0 36-37 0.0 0.0 0.0 0.2951477887705706 0.0 38-39 0.0 0.0 0.0 0.4139436226283041 0.0 40-41 0.0 0.0 0.0 0.5196934023802142 0.0 42-43 0.0 0.0 0.0 0.6348056444904211 0.0 44-45 0.0 0.0 0.0 0.7792331375913608 0.0 46-47 0.0 0.0 0.0 0.9371671337665646 0.0 48-49 0.0 0.0 0.0 1.080366762951662 0.0 50-51 0.0 0.0 0.0 1.2178875215259892 0.0 52-53 0.0 0.0 0.0 1.3638498445217317 0.0 54-55 0.0 0.0 0.0 1.527616194297186 0.0 56-57 0.0 0.0 0.0 1.7148654414631226 0.0 58-59 0.0 0.0 0.0 1.8938266071971244 0.0 60-61 0.0 0.0 0.0 2.0513001544038874 0.0 62-63 0.0 0.0 0.0 2.227805592306205 0.0 64-65 0.0 0.0 0.0 2.462174117242586 0.0 66-67 0.0 0.0 0.0 2.7382900153176024 0.0 68-69 0.0 0.0 0.0 3.0374283618146602 0.0 70-71 0.0 0.0 0.0 3.3540637691124693 0.0 72-73 0.0 0.0 0.0 3.690344999063754 0.0 74-75 0.0 0.0 0.0 4.038444419205019 0.0 76-77 0.0 0.0 0.0 4.44471389235931 0.0 78-79 0.0 0.0 0.0 4.897028262357683 0.0 80-81 0.0 0.0 0.0 5.350723979261378 0.0 82-83 0.0 0.0 0.0 5.807489355954679 0.0 84-85 0.0 0.0 0.0 6.289579425912226 0.0 86-87 0.0 0.0 0.0 6.835825385472528 0.0 88-89 0.0 0.0 0.0 7.438246119182611 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1490 0.0 53.558086 1 GCGTCAG 45 1.5133728E-5 52.7788 1 CGTCAGA 60 8.276701E-5 39.5841 2 GTATCAA 2495 0.0 38.45766 1 CGGAGAT 50 0.0016139603 38.000736 5 GGGATCC 75 6.913375E-6 37.9949 7 TCAACGC 2985 0.0 31.985544 4 ATCAACG 3005 0.0 31.930735 3 TATCAAC 3010 0.0 31.877693 2 GGAGTCT 90 2.4099001E-5 31.662415 6 CAACGCA 3075 0.0 31.203857 5 AACGCAG 3115 0.0 30.645966 6 GTGGTAT 695 0.0 28.705593 1 ACGCAGA 3455 0.0 27.630156 7 CGCAGAG 3445 0.0 27.58097 8 GAGTACT 1820 0.0 26.75806 12-13 GCAGAGT 3595 0.0 25.637259 9 GTATTAT 95 0.0012245268 25.000486 1 TCGGAGA 95 0.0012245268 25.000486 4 GTACTTT 1950 0.0 24.976109 14-15 >>END_MODULE