FastQCFastQC Report
Fri 10 Feb 2017
SRR2031748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325769
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29790.9144516513234838No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT26950.827273313298687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.7603547298852869No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18930.5810865981723246No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.536269565244084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.4024323984172834No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT11550.35454570569943733TruSeq Adapter, Index 3 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.2090438316721358No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT6710.20597417188253025RNA PCR Primer, Index 3 (95% over 23bp)
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC6470.19860698838747703No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG5390.16545466265973743TruSeq Adapter, Index 3 (96% over 28bp)
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC5260.16146410493325025No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT4700.14427401011145935No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA4600.14120435032185383No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC4340.1332232348688795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.13076750703719509No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG4150.127390881268629No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT3720.11419134417332527No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG3650.1120425823206014No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3540.10866595655203534No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.10221967099386375No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG3310.10160573903594265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG603.255991E-1063.377371
GGTATCA13550.051.917991
CGTCAGA851.2732926E-1050.3213582
GTATCAA24050.041.702571
CCAGATC905.6845056E-736.9417578
ATCAACG30900.032.6066473
TCAACGC31300.032.189954
TATCAAC31200.032.1407972
CAACGCA31600.031.8647695
CGGAGAT600.003933422331.6886841
AACGCAG31850.031.6146556
TAATGCG404.493441E-429.68533970-71
GTGGTAT7050.027.643321
ACGCAGA36300.027.4773437
CGCAGAG36450.027.1036538
CTTAGAC908.9055253E-426.4031813
CGGTGAA458.9471677E-426.3869774-75
GCAGAGT38500.025.4137389
CGTAGCT1501.0568801E-625.3314918
TCGTAGC1501.0568801E-625.3314917
Produced by FastQC (version 0.11.5)