FastQCFastQC Report
Fri 10 Feb 2017
SRR2031746_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031746_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407662
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31270.7670570227296143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24880.610309521122891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22630.5551167388670026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.3848776682643955No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.33360970608003687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.15453979031648768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.10915905823942385No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.10449833440448214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.054.6002961
CGGTATC652.5871886E-643.841332
GTATCAA23350.041.0875551
TATCAAC27450.034.6045722
TCAACGC27750.034.059324
ATCAACG27950.033.81563
GCGGTAT851.6308502E-533.5257261
CAACGCA28450.033.2213025
AACGCAG29450.032.415796
GTGGTAT4800.031.6631851
ACGCAGA32350.029.2162637
CGCAGAG32450.028.9834258
TGGTATC5400.028.1450542
GCAGAGT33550.027.183669
AGATCGT700.00838981227.1398726
ACGTCAA350.00839465927.13987252-53
GAGTACT22250.024.02011712-13
GCGTCAG1000.001652874223.7473871
CAGAGTA32350.023.12768710-11
AGTACTT22450.021.6900312-13
Produced by FastQC (version 0.11.5)