##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031746_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 407662 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.023325696287614 33.0 31.0 34.0 30.0 34.0 2 32.331237642949304 34.0 31.0 34.0 31.0 34.0 3 32.42562465964451 34.0 31.0 34.0 31.0 34.0 4 35.877116336572946 37.0 35.0 37.0 35.0 37.0 5 35.88650892160663 37.0 37.0 37.0 35.0 37.0 6 35.919163424601756 37.0 37.0 37.0 35.0 37.0 7 35.900552418425065 37.0 37.0 37.0 35.0 37.0 8 35.8971083888123 37.0 37.0 37.0 35.0 37.0 9 37.666066496264065 39.0 38.0 39.0 35.0 39.0 10-11 37.60868440031202 39.0 38.0 39.0 35.0 39.0 12-13 37.552207465988985 39.0 38.0 39.0 35.0 39.0 14-15 38.893763706207594 41.0 39.0 41.0 36.0 41.0 16-17 38.81752775583694 41.0 39.0 41.0 36.0 41.0 18-19 38.83538936668122 41.0 39.0 41.0 35.0 41.0 20-21 38.8473993161001 41.0 39.0 41.0 35.0 41.0 22-23 38.72868945351786 41.0 39.0 41.0 35.0 41.0 24-25 38.64434139066187 41.0 39.0 41.0 35.0 41.0 26-27 38.51497441507916 40.0 38.0 41.0 35.0 41.0 28-29 38.36381855556809 40.0 38.0 41.0 34.5 41.0 30-31 38.12156762219682 40.0 38.0 41.0 34.0 41.0 32-33 37.82932551967071 40.0 37.5 41.0 34.0 41.0 34-35 37.612595728814554 40.0 37.0 41.0 33.0 41.0 36-37 37.39986066888746 40.0 37.0 41.0 33.0 41.0 38-39 37.188112946509605 40.0 36.5 41.0 33.0 41.0 40-41 37.03150526661793 40.0 36.0 41.0 32.0 41.0 42-43 36.920537111626786 40.0 36.0 41.0 31.5 41.0 44-45 36.68031727264253 40.0 36.0 41.0 31.0 41.0 46-47 36.44017960957852 40.0 36.0 41.0 30.0 41.0 48-49 36.33903209031011 40.0 36.0 41.0 30.0 41.0 50-51 35.75270076681172 39.5 35.5 40.5 29.0 41.0 52-53 35.73874803145743 39.5 35.0 40.5 28.5 41.0 54-55 35.91781058818335 40.0 35.0 41.0 28.5 41.0 56-57 35.760527103335605 40.0 35.0 41.0 28.0 41.0 58-59 35.55649042589204 39.0 35.0 41.0 27.5 41.0 60-61 35.30432564232133 39.0 35.0 41.0 27.0 41.0 62-63 35.00522001069514 39.0 35.0 41.0 26.0 41.0 64-65 34.7102194955625 38.0 35.0 41.0 26.0 41.0 66-67 34.39510059804446 37.5 35.0 40.0 25.5 41.0 68-69 34.04074208535502 37.0 35.0 40.0 24.5 41.0 70-71 33.657365660767994 36.5 34.0 39.0 23.5 41.0 72-73 33.26846750494282 36.0 34.0 39.0 22.0 41.0 74-75 32.85099543249064 35.5 34.0 39.0 20.0 40.5 76-77 32.44902517281473 35.0 34.0 37.5 19.0 39.5 78-79 32.050068439049994 35.0 33.5 37.0 17.0 39.0 80-81 31.682323836904104 35.0 33.0 36.5 11.5 39.0 82-83 31.33603450898048 35.0 33.0 36.0 7.0 38.0 84-85 30.99179099351914 35.0 33.0 36.0 2.0 37.0 86-87 30.69077569162689 35.0 33.0 35.0 2.0 37.0 88-89 30.39951479411866 35.0 32.5 35.0 2.0 36.0 90-91 30.12566415314648 35.0 32.0 35.0 2.0 36.0 92-93 29.866504604304545 35.0 32.0 35.0 2.0 36.0 94-95 29.581460867090875 35.0 31.5 35.0 2.0 35.5 96-97 29.28967134537926 35.0 31.0 35.0 2.0 35.0 98-99 28.999424768558267 35.0 31.0 35.0 2.0 35.0 100-101 28.40541183627613 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1192365090735592 1101 2 -0.11001920157567469 1101 3 -0.05383194540790015 1101 4 0.016095022862202768 1101 5 -0.16315913526556614 1101 6 -0.02175233610203975 1101 7 0.004839245766476097 1101 8 -0.04752090786187324 1101 9 -0.19953800400423916 1101 10-11 0.0529765802034774 1101 12-13 0.032797884212016015 1101 14-15 -0.027203183524079577 1101 16-17 -0.08891742766450506 1101 18-19 -0.18545851771418853 1101 20-21 -0.05211720682454768 1101 22-23 -0.29008995263249204 1101 24-25 -0.47576251870513175 1101 26-27 -0.2436737120252559 1101 28-29 -0.2839120816742806 1101 30-31 -0.5248195973401479 1101 32-33 -0.3143419753428205 1101 34-35 -0.3868309444570599 1101 36-37 -0.47897178226632775 1101 38-39 -0.2184265666674392 1101 40-41 -0.46289455102969157 1101 42-43 -0.3477303537862184 1101 44-45 -0.6259135760121737 1101 46-47 -0.668163959357976 1101 48-49 -0.6841786853380114 1101 50-51 -0.2485619900971514 1101 52-53 -0.5357826702105513 1101 54-55 -0.2338468638163036 1101 56-57 -0.7223251582899266 1101 58-59 -0.6028012634841176 1101 60-61 -0.8839520728916455 1101 62-63 -0.5329212999839044 1101 64-65 -0.35374139242265557 1101 66-67 -0.735209738834449 1101 68-69 -0.8169526855396612 1101 70-71 -0.6322519120644259 1101 72-73 -0.4316701560259233 1101 74-75 -0.47455623979190875 1101 76-77 -0.8039673624937684 1101 78-79 -0.732204934416707 1101 80-81 -0.7087782063872794 1101 82-83 -0.4943874604470686 1101 84-85 -0.5784365123072952 1101 86-87 -0.7064960210649645 1101 88-89 -0.8058210492419313 1101 90-91 -1.19602368658159 1101 92-93 -0.8465244290313869 1101 94-95 -0.8337044388775112 1101 96-97 -0.6975422333295036 1101 98-99 -0.792332553380156 1101 100-101 -1.1148712364471685 1102 1 -0.02652044865708092 1102 2 0.12505095661541077 1102 3 0.25832284947993855 1102 4 0.2073542953619807 1102 5 0.3241587662448424 1102 6 0.10081447058309578 1102 7 0.18111058368764787 1102 8 0.06920980980726199 1102 9 0.2795039121634275 1102 10-11 0.2717405448115713 1102 12-13 0.21300612444795775 1102 14-15 0.32664881397346335 1102 16-17 0.17884108516059172 1102 18-19 0.10945166192652778 1102 20-21 0.13341322484941287 1102 22-23 -0.1766303047640534 1102 24-25 -0.08082999569145954 1102 26-27 0.02103716472869621 1102 28-29 0.07833208670538028 1102 30-31 -0.04772602329421005 1102 32-33 -0.08326234356160711 1102 34-35 0.19739463423630355 1102 36-37 0.3255415790706948 1102 38-39 -0.21701893417123586 1102 40-41 0.06506594588692138 1102 42-43 0.17277192523782503 1102 44-45 0.03936929108944298 1102 46-47 0.2158337169312361 1102 48-49 0.436015255139246 1102 50-51 0.42727588638868497 1102 52-53 0.3782757800531016 1102 54-55 0.4982158763749567 1102 56-57 0.49381569433317907 1102 58-59 0.35385259298205796 1102 60-61 0.3713075571020994 1102 62-63 0.5564924099097439 1102 64-65 0.700289352058526 1102 66-67 0.43142266799367945 1102 68-69 0.2942055949143523 1102 70-71 0.3974200872205813 1102 72-73 0.07926469069853681 1102 74-75 0.20348022459275938 1102 76-77 0.2016050747213569 1102 78-79 0.23399847965068687 1102 80-81 0.2538904860785358 1102 82-83 0.4105727683490734 1102 84-85 0.3621569276784058 1102 86-87 0.5742171793997812 1102 88-89 0.767040689966219 1102 90-91 0.8087309831833593 1102 92-93 0.68624883041986 1102 94-95 -0.02542845353476153 1102 96-97 -0.030659580724265112 1102 98-99 0.47426410405125097 1102 100-101 0.42014082897422966 1103 1 0.039151193133967865 1103 2 0.2683345387049627 1103 3 0.37175568530083325 1103 4 0.2551154893918266 1103 5 0.40774085579707986 1103 6 0.27693387356816856 1103 7 0.2646926732398853 1103 8 0.5229411530908479 1103 9 0.38099644947686073 1103 10-11 0.34935248511008155 1103 12-13 0.5159911990748256 1103 14-15 0.6251562766600287 1103 16-17 0.6594381000859713 1103 18-19 0.3273621096877264 1103 20-21 0.2334132248494143 1103 22-23 0.34575775493744487 1103 24-25 0.8057371684876458 1103 26-27 0.5986491050272065 1103 28-29 0.4066902956606029 1103 30-31 0.3104829319296698 1103 32-33 0.22121526837869254 1103 34-35 0.001872246176596093 1103 36-37 -0.05057782391437371 1103 38-39 -0.18119803864885142 1103 40-41 0.2650659458869242 1103 42-43 -0.07498926879202372 1103 44-45 0.09011555974615959 1103 46-47 -0.172225984561301 1103 48-49 0.16586600140790608 1103 50-51 0.17652961773196552 1103 52-53 -0.26053019009615497 1103 54-55 -0.44208263108772883 1103 56-57 -0.32857236536830925 1103 58-59 0.07922572731041555 1103 60-61 -0.019737219017308405 1103 62-63 0.1758953949843729 1103 64-65 0.14058785952121156 1103 66-67 -0.011860914095869646 1103 68-69 -0.09385410657818483 1103 70-71 -0.5548187187495586 1103 72-73 -0.572974115271613 1103 74-75 -0.17114664107888444 1103 76-77 -0.08943970139804946 1103 78-79 -0.24361346064782552 1103 80-81 -0.17596026019012 1103 82-83 -0.2939048435912248 1103 84-85 -0.2676938185902493 1103 86-87 -0.09294699970469722 1103 88-89 -0.10907871301885663 1103 90-91 -0.021119763085298615 1103 92-93 -0.4988257964458107 1103 94-95 -0.029906065475060473 1103 96-97 -0.0381222672914312 1103 98-99 -0.06304932878457237 1103 100-101 0.30521545583990317 1104 1 -0.011603460563840429 1104 2 0.0064613205261920825 1104 3 0.18937265662398772 1104 4 0.14974906309633695 1104 5 -0.015594713547208983 1104 6 0.029700937126783344 1104 7 0.15686104486491104 1104 8 0.12468524163688244 1104 9 0.0042230132870244574 1104 10-11 0.15772494863515618 1104 12-13 0.20312016100673702 1104 14-15 0.3193957534334615 1104 16-17 0.3826562788550447 1104 18-19 0.2274291900164087 1104 20-21 0.07950998822355615 1104 22-23 0.20344264509340348 1104 24-25 0.23814954480828732 1104 26-27 0.29261606796532647 1104 28-29 0.046816909781014715 1104 30-31 0.24113444962210195 1104 32-33 0.22093017698173867 1104 34-35 0.35401881669478996 1104 36-37 0.23244246788505762 1104 38-39 0.08880447686347992 1104 40-41 0.17353483738448716 1104 42-43 0.10480278218902583 1104 44-45 0.2958131951645768 1104 46-47 0.2617879346069074 1104 48-49 0.4305104756658267 1104 50-51 0.2034749472640769 1104 52-53 0.42239367373078096 1104 54-55 0.6363845834016217 1104 56-57 0.441882942362696 1104 58-59 0.5040496414475939 1104 60-61 0.7319565592822102 1104 62-63 0.5288175818030894 1104 64-65 0.7591984582131488 1104 66-67 0.6540622789277748 1104 68-69 0.6261274463314308 1104 70-71 0.6042161630213556 1104 72-73 0.5784136090282388 1104 74-75 0.6353559582838528 1104 76-77 0.8942597787294062 1104 78-79 1.0330174298435324 1104 80-81 0.8470315224696101 1104 82-83 0.9116712087314305 1104 84-85 0.9613771188573459 1104 86-87 0.5894486065337716 1104 88-89 0.7475245068369212 1104 90-91 1.026004167821963 1104 92-93 0.9948770376980782 1104 94-95 1.0272337970102683 1104 96-97 1.121067737739594 1104 98-99 0.9164240906351822 1104 100-101 1.017260567360946 1105 1 0.04306960645818236 1105 2 0.2611253018702655 1105 3 0.35629927463180167 1105 4 0.3844472671606667 1105 5 0.2662354448464441 1105 6 0.18368046406581584 1105 7 0.3468425706530951 1105 8 0.2836846056394222 1105 9 0.18516428952192143 1105 10-11 0.37394515518290206 1105 12-13 0.31495728508681964 1105 14-15 0.506477433678171 1105 16-17 0.4578932636213935 1105 18-19 0.5164597481798978 1105 20-21 0.5358873769997103 1105 22-23 0.34692442830912995 1105 24-25 0.33337123535009994 1105 26-27 0.518027952032071 1105 28-29 0.14874685920407416 1105 30-31 0.36891315279300585 1105 32-33 0.4976042131427434 1105 34-35 0.5147217453116468 1105 36-37 0.5518721901661721 1105 38-39 0.37038099341455677 1105 40-41 0.4658665252005889 1105 42-43 0.2280802890789957 1105 44-45 0.2546285741887715 1105 46-47 0.5829174358031821 1105 48-49 0.5929207545961361 1105 50-51 0.4697950962691948 1105 52-53 0.7115340171694058 1105 54-55 0.6352034452690347 1105 56-57 0.40588472921269414 1105 58-59 0.6107420003924489 1105 60-61 0.5781587459020372 1105 62-63 0.6699976599399164 1105 64-65 0.617177954866591 1105 66-67 0.5569969529033685 1105 68-69 0.34964269512757795 1105 70-71 0.6191886031315974 1105 72-73 0.4498323376390303 1105 74-75 0.6676593290703678 1105 76-77 0.8929877838173823 1105 78-79 0.6777447080772774 1105 80-81 0.972565306048768 1105 82-83 0.9745668142918653 1105 84-85 0.8478591443578125 1105 86-87 0.8593803125212318 1105 88-89 0.8063656286152927 1105 90-91 0.8604025445141694 1105 92-93 0.9492290774327792 1105 94-95 1.1840336698107734 1105 96-97 0.9925129662443943 1105 98-99 1.2223994381623662 1105 100-101 1.1411248576180704 1106 1 0.019022108825780037 1106 2 0.1033486573011686 1106 3 0.14505136904427474 1106 4 -0.07150814917211079 1106 5 -0.06841888603430135 1106 6 0.07056033585134713 1106 7 0.15573744935929312 1106 8 0.3032154572457486 1106 9 0.31463904729856296 1106 10-11 0.3605263455376715 1106 12-13 0.26201782271338914 1106 14-15 0.37808084301742895 1106 16-17 0.4912654498237643 1106 18-19 0.3932354457103173 1106 20-21 0.38300983638632147 1106 22-23 0.33105424545781403 1106 24-25 0.3036597179027325 1106 26-27 0.3925781086577018 1106 28-29 0.46112756875459837 1106 30-31 0.7163925729139393 1106 32-33 0.4405779146070046 1106 34-35 0.45846764754811886 1106 36-37 0.4789502115838147 1106 38-39 0.47610329253307526 1106 40-41 0.1700881322523884 1106 42-43 0.48674933548388566 1106 44-45 0.6735766327594703 1106 46-47 0.6710575975282538 1106 48-49 0.9632238069746677 1106 50-51 1.1246942002249085 1106 52-53 1.0319062762209086 1106 54-55 0.8517656948501511 1106 56-57 0.8416172272093405 1106 58-59 0.8654298418727393 1106 60-61 0.9550510020395748 1106 62-63 1.0787796224954675 1106 64-65 1.2884499006668406 1106 66-67 1.2177276296717778 1106 68-69 1.0940442395291186 1106 70-71 1.157867848414611 1106 72-73 1.1733953885605786 1106 74-75 0.844686356097391 1106 76-77 0.9087450505180428 1106 78-79 1.1594522916716556 1106 80-81 1.2433418777687315 1106 82-83 1.0686405376108716 1106 84-85 1.037933450469854 1106 86-87 0.533777485975012 1106 88-89 0.5934021260291473 1106 90-91 0.6724663603515744 1106 92-93 0.6433282979470896 1106 94-95 0.622534434726635 1106 96-97 0.8067950380736342 1106 98-99 0.5188990374921545 1106 100-101 0.688495004044821 1107 1 -0.10507055526688802 1107 2 -0.004373350420841859 1107 3 -0.001975657982754342 1107 4 0.08223702457306814 1107 5 0.10833787072245826 1107 6 0.13812790341891912 1107 7 -0.024262550640706593 1107 8 0.022983797014084928 1107 9 0.11950391216343093 1107 10-11 0.016047581058906246 1107 12-13 0.0454155061110697 1107 14-15 -0.06392687899029426 1107 16-17 0.021612940171259254 1107 18-19 0.3080230904979544 1107 20-21 0.09343454681103935 1107 22-23 0.14603494043850418 1107 24-25 0.2437755480185615 1107 26-27 0.23018428626387788 1107 28-29 0.12931289693992198 1107 30-31 -0.011329434808068584 1107 32-33 0.04019181422090412 1107 34-35 0.194452203532677 1107 36-37 0.42586140849500964 1107 38-39 0.3788446052743879 1107 40-41 0.2738719160361782 1107 42-43 0.38196169069624375 1107 44-45 0.5009897601725939 1107 46-47 0.3224861689568215 1107 48-49 0.12364851739937421 1107 50-51 0.012840918371622934 1107 52-53 0.3752267395413753 1107 54-55 0.40717110025556025 1107 56-57 0.23343189902401207 1107 58-59 0.021954938397833246 1107 60-61 0.2880239750125426 1107 62-63 0.22495722867306966 1107 64-65 0.12370086591780449 1107 66-67 0.10093226287641244 1107 68-69 -0.14750016201528382 1107 70-71 0.19215356270032657 1107 72-73 0.37436063952582543 1107 74-75 0.1276593290703687 1107 76-77 0.5570848188578097 1107 78-79 0.4373673495867152 1107 80-81 0.296662341089192 1107 82-83 0.20871775768809187 1107 84-85 0.3346515971880031 1107 86-87 0.2867118489093734 1107 88-89 0.42286158548860797 1107 90-91 0.2867906846758963 1107 92-93 0.3139460585648486 1107 94-95 0.4687506509428516 1107 96-97 0.6995749608535569 1107 98-99 0.7332619718550859 1107 100-101 0.6470278225776411 1108 1 -0.35611437429075465 1108 2 -0.20191521005746438 1108 3 -0.03559161808249911 1108 4 -0.20381926327873146 1108 5 -0.09370701705809381 1108 6 0.07304062162340585 1108 7 0.12805665135430644 1108 8 -0.00507462835107475 1108 9 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-0.25975727911312063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1996.0 3 319.0 4 268.0 5 244.0 6 314.0 7 352.0 8 503.0 9 592.0 10 726.0 11 898.0 12 1172.0 13 1671.0 14 1838.0 15 2378.0 16 2946.0 17 4130.0 18 3737.0 19 2223.0 20 2048.0 21 1902.0 22 1927.0 23 2109.0 24 2344.0 25 2525.0 26 2862.0 27 3352.0 28 3954.0 29 4739.0 30 5644.0 31 6985.0 32 8832.0 33 11447.0 34 16132.0 35 28282.0 36 44313.0 37 81437.0 38 113272.0 39 37199.0 40 50.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.233337673600445 20.626177706914046 14.842239479561027 22.29824513992448 2 16.199582830707392 22.881661994599895 39.22079262165595 21.697962553036763 3 18.50239528313475 27.319493919665888 31.46689595872743 22.711214838471932 4 13.569449085084221 20.31830421195385 37.36340140332947 28.748845299632464 5 13.715317439846714 36.72721913113969 34.499920164093496 15.057543264920103 6 27.66706780951445 37.36105312277427 19.60851732691505 15.363361740796227 7 23.72637825251875 34.386681927367576 24.28342658407549 17.60351323603818 8 23.43302569341775 33.796348597446865 22.862033566969394 19.908592142165986 9 25.205424281567563 17.678767012273884 22.96994641532787 34.145862290830685 10-11 23.11924127559007 27.411313342131916 29.003652435468524 20.46579294680949 12-13 24.640299033470576 26.414459506088857 28.73960683091077 20.2056346295298 14-15 22.160197692441848 27.68357418502224 28.05191391732191 22.10431420521399 16-17 20.368380000122833 29.82649658213621 28.801690199667117 21.003433218073837 18-19 20.056771981025236 28.083214802477237 31.906909673346156 19.953103543151375 20-21 21.079554805540614 27.519079938000946 31.675521919514228 19.725843336944212 22-23 20.978065974013216 27.376145211603177 31.505415960504017 20.140372853879597 24-25 20.515553416250505 27.596707707760558 31.730491768655444 20.157247107333493 26-27 20.293556757048815 27.890593437521872 31.811969443376796 20.003880362052513 28-29 20.32803071051216 27.983308649980227 31.743528908995795 19.945131730511818 30-31 20.73158602398099 27.861173018007236 31.390050282725213 20.01719067528656 32-33 20.434673166299323 28.146192619151407 31.200569741099333 20.218564473449945 34-35 20.78390821819057 28.115056387307053 31.05016830840535 20.05086708609703 36-37 20.675413290889733 27.97175830235417 31.081832885685966 20.27099552107013 38-39 20.511394195701666 28.20146323133938 31.13664439156051 20.150498181398444 40-41 20.594414911579463 28.103376079238952 30.997474049760115 20.304734959421474 42-43 20.721953040390375 28.153295301337668 30.690126006566782 20.434625651705176 44-45 20.794058805251968 28.309223122713377 30.54932962602257 20.347388446012083 46-47 20.70925400115933 28.538901388247545 30.226069186406374 20.52577542418675 48-49 20.765599839834724 28.712941820946526 29.961223790438968 20.560234548779786 50-51 20.688410113318586 29.013709498153524 29.60676841227481 20.691111976253083 52-53 20.783449861124414 29.17946753307351 29.331481658844936 20.70560094695714 54-55 20.81298237600957 29.0905117268171 29.299485056319664 20.797020840853673 56-57 20.720839100037946 29.202592161866438 29.26780146407778 20.80876727401784 58-59 20.646601529259645 29.096946024035432 29.35517896414577 20.901273482559148 60-61 20.692077127760815 29.014389929137057 29.528026532774877 20.76550641032725 62-63 20.571997632537382 29.07570723033889 29.370780501342136 20.981514635781593 64-65 20.566073654669278 29.184910434104435 29.267690569328366 20.981325341897925 66-67 20.580588669457693 29.075977830639943 29.194733487498723 21.148700012403644 68-69 20.54441218516499 29.144744275615427 29.123988622463266 21.18685491675632 70-71 20.587494888492387 29.074255316274915 29.099797504479092 21.238452290753603 72-73 20.517893061603413 29.066309075592688 29.050099590096494 21.3656982727074 74-75 20.565196358894898 29.148239731459903 28.902560904039156 21.384003005606044 76-77 20.539612478216178 29.08046781643498 28.79542057670178 21.584499128647064 78-79 20.386345158588437 29.135298177700598 28.935481414707592 21.542875249003373 80-81 20.454749211238305 29.14973392725339 28.731926641784906 21.663590219723403 82-83 20.42677422405524 29.29117011647001 28.59601007613599 21.68604558333876 84-85 20.585540777855556 29.104373134878237 28.553095334585958 21.75699075268025 86-87 20.32053313289266 29.050387739712065 28.699248101831724 21.929831025563555 88-89 20.611061990372335 28.857451616072304 28.58274388446802 21.94874250908734 90-91 20.443623510744256 28.908970106196012 28.687784694853786 21.959621688205942 92-93 20.454369585537606 28.70345235887238 28.834142572705645 22.008035482884374 94-95 20.409636867563684 28.646459486375935 28.77810128805117 22.165802358009213 96-97 20.402044108943837 28.59591522006586 28.680759001912975 22.32128166907733 98-99 20.404825490376645 28.578690487153512 28.535469809988644 22.4810142124812 100-101 20.311304217507576 28.4422873526366 28.589942498025327 22.656465931830493 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2956.0 1 1813.5 2 684.0 3 628.5 4 512.0 5 532.0 6 692.5 7 1162.0 8 1728.0 9 1849.0 10 1451.0 11 1015.0 12 908.0 13 881.5 14 891.5 15 914.5 16 944.5 17 1002.0 18 1031.5 19 1102.0 20 1205.5 21 1315.5 22 1428.5 23 1625.0 24 1907.0 25 2282.5 26 2768.0 27 3386.0 28 4066.5 29 5013.5 30 6173.5 31 7524.5 32 8873.5 33 9693.0 34 10493.5 35 11206.5 36 11976.5 37 12662.5 38 12762.0 39 12755.5 40 12936.5 41 13034.0 42 13060.0 43 13275.0 44 13672.0 45 13926.0 46 13916.5 47 14055.0 48 14294.5 49 14014.5 50 13248.5 51 12744.5 52 12166.0 53 11482.0 54 10687.5 55 9621.5 56 8537.0 57 7521.0 58 6728.5 59 5991.0 60 5058.0 61 4164.0 62 3409.0 63 2501.0 64 1760.5 65 1352.5 66 1046.5 67 808.5 68 607.0 69 429.5 70 314.0 71 232.0 72 165.5 73 115.0 74 96.5 75 93.0 76 68.5 77 45.0 78 35.5 79 25.0 80 17.5 81 10.0 82 8.5 83 10.5 84 10.5 85 6.0 86 3.5 87 4.0 88 2.0 89 1.5 90 2.5 91 3.0 92 2.0 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15233207902625212 2 0.15527569407989952 3 0.1501243677360166 4 0.15453979031648768 5 0.14153882382954508 6 0.12068821719954274 7 0.12657544730683754 8 0.11676339712801292 9 0.11185737203860062 10-11 0.09799785116101083 12-13 0.11860315653654252 14-15 0.13859520877589768 16-17 0.14951111459984007 18-19 0.14595424641001614 20-21 0.137123401249074 22-23 0.13050026737836737 24-25 0.1149236377194833 26-27 0.11860315653654252 28-29 0.12387713350766076 30-31 0.11394243270160084 32-33 0.11271592642924777 34-35 0.12816990546089652 36-37 0.1308682192600733 38-39 0.11798990340036598 40-41 0.1198296628088956 42-43 0.11308387831095368 44-45 0.13209472553242638 46-47 0.13000966486942614 48-49 0.14288798072913345 50-51 0.13135882176901453 52-53 0.11357448081989491 54-55 0.10621544318577644 56-57 0.12547159166171976 58-59 0.11565954148289515 60-61 0.11467833646501269 62-63 0.1165180958735423 64-65 0.13724605187630928 66-67 0.12841520671536716 68-69 0.13344388243201477 70-71 0.12081086782677808 72-73 0.12093351845401337 74-75 0.1037624306410703 76-77 0.13160412302348515 78-79 0.13221737615966167 80-81 0.13160412302348515 82-83 0.1377366543852505 84-85 0.12682074856130815 86-87 0.137123401249074 88-89 0.12314122974424892 90-91 0.13184942427795576 92-93 0.1453409932738396 94-95 0.12179207284466052 96-97 0.1086684557304826 98-99 0.1102629138845416 100-101 0.1551530434526642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 407662.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.21465460474224 #Duplication Level Percentage of deduplicated Percentage of total 1 73.94389619566681 49.70113442920658 2 17.46159969294707 23.47350784415421 3 5.243191101610576 10.572578367642384 4 1.775826532098614 4.774462679717821 5 0.6753534921153356 2.26968258543194 6 0.3100594515334843 1.2504323365055379 7 0.17772548357680745 0.836202989515313 8 0.1010581333433533 0.5434070022138782 9 0.0685166648893857 0.4144791568688036 >10 0.23032695070029255 2.4551378101762844 >50 0.006223150767580764 0.2945232083257849 >100 0.004026744603342095 0.6003647676146934 >500 3.6606769121291766E-4 0.1550122145890397 >1k 0.0018303384560645884 2.6590746080377015 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3127 0.7670570227296143 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2488 0.610309521122891 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2263 0.5551167388670026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1569 0.3848776682643955 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1360 0.33360970608003687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 630 0.15453979031648768 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 445 0.10915905823942385 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.10449833440448214 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.226506272353077E-4 0.0 22-23 0.0 0.0 0.0 9.812050178824615E-4 0.0 24-25 0.0 0.0 0.0 0.001594458154059 0.0 26-27 0.0 0.0 0.0 0.005028675716647615 0.0 28-29 0.0 0.0 0.0 0.013246267741413228 0.0 30-31 0.0 0.0 0.0 0.026247234228355842 0.0 32-33 0.0 0.0 0.0 0.06206121738106568 0.0 34-35 0.0 0.0 0.0 0.1198296628088956 0.0 36-37 0.0 0.0 0.0 0.19256148475943308 0.0 38-39 0.0 0.0 0.0 0.28221909326844297 0.0 40-41 0.0 0.0 0.0 0.37248995491362946 0.0 42-43 0.0 0.0 0.0 0.4617796115409334 0.0 44-45 0.0 0.0 0.0 0.5595321614474736 0.0 46-47 0.0 0.0 0.0 0.6678326652962503 0.0 48-49 0.0 0.0 0.0 0.7657078658300258 0.0 50-51 0.0 0.0 0.0 0.86701728392639 0.0 52-53 0.0 0.0 0.0 0.9672228463776363 0.0 54-55 0.0 0.0 0.0 1.0872978104410027 0.0 56-57 0.0 0.0 0.0 1.2304310924246065 0.0 58-59 0.0 0.0 0.0 1.3560253347135618 0.0 60-61 0.0 0.0 0.0 1.4786759619488694 0.0 62-63 0.0 0.0 0.0 1.609666831836178 0.0 64-65 0.0 0.0 0.0 1.766291682815666 0.0 66-67 0.0 0.0 0.0 1.959589071338511 0.0 68-69 0.0 0.0 0.0 2.198389842565655 0.0 70-71 0.0 0.0 0.0 2.4311807330582687 0.0 72-73 0.0 0.0 0.0 2.672066564948413 0.0 74-75 0.0 0.0 0.0 2.9225191457629114 0.0 76-77 0.0 0.0 0.0 3.2043702871496484 0.0 78-79 0.0 0.0 0.0 3.5041284201127407 0.0 80-81 0.0 0.0 0.0 3.807320770638421 0.0 82-83 0.0 0.0 0.0 4.128420112740457 0.0 84-85 0.0 0.0 0.0 4.489258258066732 0.0 86-87 0.0 0.0 0.0 4.898666051778189 0.0 88-89 0.0 0.0 0.0 5.392702778282008 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1270 0.0 54.600296 1 CGGTATC 65 2.5871886E-6 43.84133 2 GTATCAA 2335 0.0 41.087555 1 TATCAAC 2745 0.0 34.604572 2 TCAACGC 2775 0.0 34.05932 4 ATCAACG 2795 0.0 33.8156 3 GCGGTAT 85 1.6308502E-5 33.525726 1 CAACGCA 2845 0.0 33.221302 5 AACGCAG 2945 0.0 32.41579 6 GTGGTAT 480 0.0 31.663185 1 ACGCAGA 3235 0.0 29.216263 7 CGCAGAG 3245 0.0 28.983425 8 TGGTATC 540 0.0 28.145054 2 GCAGAGT 3355 0.0 27.18366 9 AGATCGT 70 0.008389812 27.139872 6 ACGTCAA 35 0.008394659 27.139872 52-53 GAGTACT 2225 0.0 24.020117 12-13 GCGTCAG 100 0.0016528742 23.747387 1 CAGAGTA 3235 0.0 23.127687 10-11 AGTACTT 2245 0.0 21.69003 12-13 >>END_MODULE