FastQCFastQC Report
Fri 10 Feb 2017
SRR2031746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407662
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41481.0175096035441125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33040.8104753447709131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.5345114334914709No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.40793598618463334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16020.3929726096619258No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.18422124210743215No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT6780.16631425053107723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.15723810411566447No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.13050026737836737No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.11578219211013044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.062.6152531
GTATCAA22350.046.5448271
GTGGTAT4350.043.679291
TGGTATC4550.039.6713522
TATCAAC27850.037.3528482
ATCAACG28550.036.104263
TCAACGC28600.036.041144
CAACGCA29600.034.8192635
AACGCAG30700.033.8810846
ACGCAGA34400.030.2368987
CGCAGAG34250.030.230658
GCAGAGT35300.028.5241499
CGTCAGA1205.3694257E-627.709052
GAGTATA950.001225012225.0006471
GCGTCAG1000.001651573223.7506141
CAGAGTA34950.023.17582910-11
GAGTACT28150.022.69875112-13
CCGCCAT1100.002875831721.5888189
GTAGGAG1553.8976927E-521.4521683
AGAGTAC34050.020.85845810-11
Produced by FastQC (version 0.11.5)