##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031746_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 407662 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7346061197757 34.0 31.0 34.0 31.0 34.0 2 32.88771089775353 34.0 31.0 34.0 31.0 34.0 3 32.93149471866399 34.0 31.0 34.0 31.0 34.0 4 36.348428354862605 37.0 37.0 37.0 35.0 37.0 5 36.32134219034396 37.0 37.0 37.0 35.0 37.0 6 36.35440389342151 37.0 37.0 37.0 35.0 37.0 7 36.30502229788403 37.0 37.0 37.0 35.0 37.0 8 36.30385711692529 37.0 37.0 37.0 35.0 37.0 9 38.147067914105314 39.0 39.0 39.0 37.0 39.0 10-11 38.12305537430518 39.0 39.0 39.0 37.0 39.0 12-13 38.05632116802646 39.0 39.0 39.0 35.0 39.0 14-15 39.481695620391406 41.0 39.0 41.0 36.0 41.0 16-17 39.45686500090761 41.0 39.0 41.0 36.5 41.0 18-19 39.49518964239983 41.0 39.0 41.0 36.0 41.0 20-21 39.50119952313436 41.0 39.0 41.0 36.0 41.0 22-23 39.40065667145822 41.0 39.0 41.0 36.0 41.0 24-25 39.32098773003125 41.0 39.0 41.0 36.0 41.0 26-27 39.16531219490656 41.0 39.0 41.0 35.0 41.0 28-29 39.028836388969296 40.5 39.0 41.0 35.0 41.0 30-31 38.75955080434281 40.0 38.0 41.0 35.0 41.0 32-33 38.44882525229234 40.0 38.0 41.0 35.0 41.0 34-35 38.24819703577964 40.0 38.0 41.0 34.5 41.0 36-37 38.07720734333836 40.0 37.5 41.0 34.0 41.0 38-39 38.07782672900589 40.0 38.0 41.0 34.0 41.0 40-41 37.93232138389156 40.0 38.0 41.0 34.0 41.0 42-43 37.768032095216135 40.0 37.5 41.0 33.5 41.0 44-45 37.479366484980204 40.0 37.0 41.0 33.0 41.0 46-47 37.09802851381782 40.0 37.0 41.0 32.5 41.0 48-49 36.710306577507836 40.0 36.5 41.0 31.0 41.0 50-51 36.474481310497424 40.0 36.0 41.0 31.0 41.0 52-53 36.48451535831154 40.0 36.0 41.0 31.0 41.0 54-55 36.38879880881711 40.0 36.0 41.0 31.0 41.0 56-57 36.19539103472975 40.0 35.5 41.0 30.5 41.0 58-59 35.98554807659286 39.5 35.0 41.0 30.0 41.0 60-61 35.72332962110768 39.0 35.0 41.0 29.0 41.0 62-63 35.439723349245206 39.0 35.0 41.0 28.5 41.0 64-65 35.113518061531366 38.5 35.0 41.0 28.0 41.0 66-67 34.757037692990764 37.5 35.0 40.0 27.5 41.0 68-69 34.360405188612134 37.0 35.0 40.0 26.0 41.0 70-71 33.99711403074115 36.5 35.0 39.5 26.0 41.0 72-73 33.59801870176764 36.0 34.0 39.0 25.5 41.0 74-75 33.20827916263964 35.5 34.0 39.0 25.0 40.5 76-77 32.28266922107039 35.0 33.0 37.0 22.5 39.0 78-79 32.436525847393185 35.0 34.0 37.0 22.5 39.0 80-81 32.1848038325868 35.0 34.0 36.5 21.5 39.0 82-83 31.898172996256704 35.0 34.0 36.0 20.5 38.0 84-85 31.58820297207 35.0 34.0 36.0 19.5 37.0 86-87 31.315989716971412 35.0 33.0 35.5 17.5 37.0 88-89 31.063551422501973 35.0 33.0 35.0 11.5 36.0 90-91 30.84283793927322 35.0 33.0 35.0 6.0 36.0 92-93 30.646203227183303 35.0 33.0 35.0 2.0 36.0 94-95 30.4440357452988 35.0 33.0 35.0 2.0 36.0 96-97 30.246677394508197 35.0 33.0 35.0 2.0 35.0 98-99 30.023369850513415 35.0 33.0 35.0 2.0 35.0 100-101 29.366946146562594 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0459888909859032 1101 2 -0.00445538455626604 1101 3 0.06403479817829805 1101 4 0.036805190036240276 1101 5 -0.0014375773392529823 1101 6 0.002335494836167129 1101 7 -0.05461679178488765 1101 8 -0.042006262636881786 1101 9 -0.2853794078284295 1101 10-11 -0.10171354382818976 1101 12-13 0.02508733013787179 1101 14-15 -0.13314775932638412 1101 16-17 -0.16379939833643675 1101 18-19 -0.0709714272349018 1101 20-21 0.009162630659915294 1101 22-23 -0.07345756800018677 1101 24-25 0.03182532109600089 1101 26-27 0.17050616217828463 1101 28-29 0.10117729564787226 1101 30-31 0.24563070824418531 1101 32-33 0.28735104725854654 1101 34-35 0.3530571741899635 1101 36-37 0.3979437548522071 1101 38-39 0.4989309560940214 1101 40-41 0.363835542937764 1101 42-43 0.22674430899127884 1101 44-45 0.3532271427620657 1101 46-47 0.5678534946343703 1101 48-49 0.4534363131789334 1101 50-51 0.08467393648680144 1101 52-53 0.23259438391348652 1101 54-55 0.1006379761046503 1101 56-57 -0.11903652571889012 1101 58-59 0.23090837241678486 1101 60-61 0.29330057718601665 1101 62-63 0.31009956485647905 1101 64-65 0.31823093431423644 1101 66-67 0.3234587885693898 1101 68-69 0.23749730226462162 1101 70-71 0.22773929809176252 1101 72-73 0.13218915060398473 1101 74-75 0.09771056981613668 1101 76-77 -0.15848558461927809 1101 78-79 -0.2918387535138578 1101 80-81 -0.3408614678200408 1101 82-83 -0.12345779322653527 1101 84-85 0.08681166857117617 1101 86-87 0.13246288786329785 1101 88-89 -0.03302019439126269 1101 90-91 -0.2298881725801536 1101 92-93 -0.4823353695347805 1101 94-95 -0.20239549154053904 1101 96-97 -0.1296852401876798 1101 98-99 -0.3772191237511642 1101 100-101 -0.3221278453887244 1102 1 0.18981765137557005 1102 2 0.16892025222986007 1102 3 0.18033652308668735 1102 4 0.08929424178349166 1102 5 0.1706600185138143 1102 6 0.037030284361591725 1102 7 0.22513117496816903 1102 8 0.16011188911930674 1102 9 -0.1237921346136659 1102 10-11 0.1175464436595206 1102 12-13 0.06055966904214927 1102 14-15 0.1955277255248049 1102 16-17 0.3053846216832241 1102 18-19 0.3038073733728339 1102 20-21 0.38665838541905373 1102 22-23 0.4359177112919781 1102 24-25 0.38440374633507446 1102 26-27 0.30523670110044065 1102 28-29 0.35893938341263976 1102 30-31 0.39480847211941494 1102 32-33 0.3716123753486329 1102 34-35 0.4422385130048738 1102 36-37 0.509875116010484 1102 38-39 0.3373704949268017 1102 40-41 0.1032000974109053 1102 42-43 0.4625339237915256 1102 44-45 0.4118472160483222 1102 46-47 0.28218453404807775 1102 48-49 0.49764044223426396 1102 50-51 0.49637739524093405 1102 52-53 0.3453479811965323 1102 54-55 0.464848361304405 1102 56-57 0.5001162135786288 1102 58-59 0.5630828294728261 1102 60-61 0.5155501079751872 1102 62-63 0.46453695872546064 1102 64-65 0.3318335797695866 1102 66-67 0.27689966800454613 1102 68-69 0.5670843967323975 1102 70-71 0.5047256239475146 1102 72-73 0.5493756730814141 1102 74-75 0.4355825869758476 1102 76-77 0.41733441716819186 1102 78-79 0.7659858956951879 1102 80-81 0.692483781978865 1102 82-83 0.5623318215737072 1102 84-85 0.7416423058041737 1102 86-87 0.9387190322908623 1102 88-89 0.4640349490531932 1102 90-91 0.29821532192337585 1102 92-93 0.49042805883236795 1102 94-95 0.33289810038009904 1102 96-97 0.34188505206364184 1102 98-99 0.5092631356107091 1102 100-101 0.5527582034092013 1103 1 0.12713108421139196 1103 2 0.10026353581194769 1103 3 0.1564559260717644 1103 4 -0.039063967171735214 1103 5 -0.09799669790409382 1103 6 0.13255267242129776 1103 7 0.15945953317712735 1103 8 0.15115666523870885 1103 9 0.162775029565438 1103 10-11 0.18620316007743298 1103 12-13 0.017276086952598746 1103 14-15 -0.1865618267139908 1103 16-17 -0.07222731861529041 1103 18-19 0.19932976143253 1103 20-21 0.22397181825486712 1103 22-23 0.1627833829337746 1103 24-25 0.051567925439549356 1103 26-27 0.1604605816974498 1103 28-29 0.3440140102783147 1103 30-31 0.22316668107463755 1103 32-33 -0.1254025500245035 1103 34-35 0.04373105031830704 1103 36-37 0.06211392198063237 1103 38-39 -0.09248025134185411 1103 40-41 0.09573741084373921 1103 42-43 -0.06433174785026807 1103 44-45 -0.17471994813078595 1103 46-47 -0.04169606296684947 1103 48-49 -0.24116552791499402 1103 50-51 -0.3245181271471296 1103 52-53 -0.5382341083557165 1103 54-55 -0.34112178794932646 1103 56-57 -0.48645095060047794 1103 58-59 -0.6264694093331471 1103 60-61 -0.959076757696451 1103 62-63 -0.5682988621700602 1103 64-65 -0.6532410470960812 1103 66-67 -1.0499660036372447 1103 68-69 -0.9194827674467021 1103 70-71 -1.0131848238136811 1103 72-73 -0.8088332821424586 1103 74-75 -0.8300890548151969 1103 76-77 -0.8438596126825537 1103 78-79 -0.9205812684839145 1103 80-81 -0.8881132329465053 1103 82-83 -0.6899069843964369 1103 84-85 -0.7404472464346306 1103 86-87 -0.5538182811419787 1103 88-89 -0.5673083345288994 1103 90-91 -0.6182025885243831 1103 92-93 -0.9677808963915098 1103 94-95 -1.0178481682766183 1103 96-97 -1.0670701718169617 1103 98-99 -0.7474532822997375 1103 100-101 -0.8591820950982587 1104 1 0.19491575635628777 1104 2 0.17750653430264407 1104 3 0.1711800582200098 1104 4 0.06164876132064023 1104 5 0.03327950534929869 1104 6 0.001494480236154061 1104 7 0.10758128397370825 1104 8 0.09734985658534612 1104 9 0.264896444960371 1104 10-11 0.19588788253256695 1104 12-13 0.20747816644279027 1104 14-15 0.2259486545873628 1104 16-17 0.08307202735150554 1104 18-19 0.17374700107701102 1104 20-21 0.2511393513757838 1104 22-23 0.2554632420650833 1104 24-25 0.17087867506221954 1104 26-27 0.14371984716785846 1104 28-29 0.17619579796907203 1104 30-31 0.3690580109421546 1104 32-33 0.47697460912358025 1104 34-35 0.43245736257723877 1104 36-37 0.6084328889435753 1104 38-39 0.6697157909187581 1104 40-41 0.7630399431261452 1104 42-43 0.7106472895470191 1104 44-45 0.34909776107599555 1104 46-47 0.5569539454181935 1104 48-49 0.7744599961500711 1104 50-51 0.5643213831664724 1104 52-53 0.5176899231720427 1104 54-55 0.5015664562230668 1104 56-57 0.5246592288394041 1104 58-59 0.5697447446162087 1104 60-61 1.010938335377503 1104 62-63 0.7306152750092068 1104 64-65 0.5703418809602638 1104 66-67 0.36519834316805344 1104 68-69 0.47220765683637467 1104 70-71 0.5518663249369453 1104 72-73 0.617202270431747 1104 74-75 0.7497239587685627 1104 76-77 0.7089116433966041 1104 78-79 0.8041378326396824 1104 80-81 0.8901904732416597 1104 82-83 0.8449957491269515 1104 84-85 0.8876594112879879 1104 86-87 0.9277094733440236 1104 88-89 0.8428124100627521 1104 90-91 0.8027600141923692 1104 92-93 0.7641996503131665 1104 94-95 0.8316319591760077 1104 96-97 0.5163064842286538 1104 98-99 0.6505175377572812 1104 100-101 0.35318164798408347 1105 1 0.14506567004234228 1105 2 0.08177377437653632 1105 3 0.0709347449155473 1105 4 0.02764561161895074 1105 5 0.028615524005225268 1105 6 0.10487435243095433 1105 7 0.0868280812722304 1105 8 0.16892228458134895 1105 9 0.05659970671875669 1105 10-11 0.15900760148226567 1105 12-13 0.2209893266838563 1105 14-15 0.1146490997855878 1105 16-17 0.23926240259001474 1105 18-19 0.3235925445519783 1105 20-21 0.239782253947034 1105 22-23 0.3017623425829612 1105 24-25 0.17794278966290733 1105 26-27 0.34701567684167856 1105 28-29 0.5323392305381986 1105 30-31 0.5611519568520862 1105 32-33 0.5458932620203996 1105 34-35 0.49834745615972054 1105 36-37 0.4380764275122786 1105 38-39 0.6969311820571846 1105 40-41 0.5199639868585422 1105 42-43 0.5181520595279423 1105 44-45 0.5340783782963854 1105 46-47 0.565517196879469 1105 48-49 0.30764164913774295 1105 50-51 0.30317628488973014 1105 52-53 0.6425157810114683 1105 54-55 0.7202814385059924 1105 56-57 0.40494785054206517 1105 58-59 0.38940539454502954 1105 60-61 0.3857074077297824 1105 62-63 0.20969928724458953 1105 64-65 0.40072363086184026 1105 66-67 0.5563420785931115 1105 68-69 0.6572602023404741 1105 70-71 0.6866220716282427 1105 72-73 0.5365744500858796 1105 74-75 0.4169117900172452 1105 76-77 0.19134978507261025 1105 78-79 0.312572370330102 1105 80-81 0.0737325247877294 1105 82-83 0.35191222146106327 1105 84-85 0.6019754111668512 1105 86-87 0.5193763923906296 1105 88-89 0.661045851414702 1105 90-91 0.6456989281509955 1105 92-93 0.36915437335141377 1105 94-95 0.24899014688611132 1105 96-97 0.10781818961040557 1105 98-99 0.32075285681600363 1105 100-101 0.29434809760399716 1106 1 0.08808308098428341 1106 2 0.06286942528350892 1106 3 0.03592345330048374 1106 4 0.08550239023044526 1106 5 -9.431791216485408E-5 1106 6 0.0656305263946706 1106 7 -0.07958847004998404 1106 8 -0.04323623512433983 1106 9 0.08960036236091895 1106 10-11 0.10308496725775029 1106 12-13 0.10561816036929628 1106 14-15 0.07449101717467244 1106 16-17 0.16284730825039162 1106 18-19 0.19614299257412426 1106 20-21 0.17969993443075083 1106 22-23 0.1078015354146089 1106 24-25 0.27803020861825445 1106 26-27 0.22550697137070586 1106 28-29 0.15823345360223584 1106 30-31 0.05937079563695846 1106 32-33 0.004085146627375025 1106 34-35 0.12745432583262328 1106 36-37 -0.06721241928600108 1106 38-39 0.026176465076062527 1106 40-41 0.3144748049542727 1106 42-43 0.09599499680484058 1106 44-45 -0.26580506309649365 1106 46-47 -0.20827532879985 1106 48-49 -0.07872099382867503 1106 50-51 -0.05007278628387013 1106 52-53 0.3145089331933022 1106 54-55 0.0949008018045987 1106 56-57 0.19307708489770903 1106 58-59 0.05394608078383101 1106 60-61 0.15635285101673446 1106 62-63 0.2304102947480473 1106 64-65 0.331873918615905 1106 66-67 0.2166939398883656 1106 68-69 -8.260510287954048E-4 1106 70-71 -0.02913883752889035 1106 72-73 -0.25086635999643647 1106 74-75 -0.10300151951870617 1106 76-77 -0.18716336419525703 1106 78-79 -0.24760829551094332 1106 80-81 -0.2566892716717959 1106 82-83 -0.24745034865621562 1106 84-85 -0.09627620972628037 1106 86-87 -0.14740440457884318 1106 88-89 -0.010087681039586016 1106 90-91 0.0523500536700503 1106 92-93 0.09476448481058242 1106 94-95 -0.16552868461385017 1106 96-97 -0.10634003869876452 1106 98-99 -0.19214872401010297 1106 100-101 0.09577958299774636 1107 1 0.10290933581053707 1107 2 0.1278115102255981 1107 3 0.10796978534681756 1107 4 0.0663518110798691 1107 5 0.03697131915347285 1107 6 0.05937570013984583 1107 7 0.07238064191913196 1107 8 0.007574575686476237 1107 9 0.1177856905462491 1107 10-11 0.08181083598361738 1107 12-13 0.23689229164342862 1107 14-15 0.08313966582331744 1107 16-17 0.07999016539324089 1107 18-19 0.10564106207218771 1107 20-21 0.20024047497129516 1107 22-23 0.27154670915978585 1107 24-25 0.1655977761858125 1107 26-27 0.3352367011004418 1107 28-29 0.21861955398833288 1107 30-31 0.10446732073349096 1107 32-33 0.0206102431524684 1107 34-35 -0.1197657513874546 1107 36-37 0.0406640285904416 1107 38-39 0.24474789364748517 1107 40-41 0.21667557715504415 1107 42-43 0.2048366956465415 1107 44-45 -0.044762592054020445 1107 46-47 -0.01267687320139288 1107 48-49 0.1846766895690095 1107 50-51 0.39247547626438717 1107 52-53 0.2536209023052649 1107 54-55 0.17825987516367547 1107 56-57 0.2571311389517632 1107 58-59 0.21220862904637983 1107 60-61 0.314036248700134 1107 62-63 0.12376936810712635 1107 64-65 0.15654573328771448 1107 66-67 0.3518676850621105 1107 68-69 0.204888234685491 1107 70-71 0.3467298883398371 1107 72-73 0.4711027519726798 1107 74-75 0.1976934611762715 1107 76-77 0.17550072846884035 1107 78-79 0.2337044458017985 1107 80-81 0.3012257862432648 1107 82-83 0.31749173628586647 1107 84-85 0.29221799876792787 1107 86-87 0.2596998425254 1107 88-89 0.5747385737866679 1107 90-91 0.10478248610248286 1107 92-93 0.17055599060208948 1107 94-95 0.3681006590154894 1107 96-97 0.2720769497182225 1107 98-99 0.21290919104781025 1107 100-101 0.4689842162023794 1108 1 0.02164107788392755 1108 2 -0.08571182429524526 1108 3 -0.06715668419361975 1108 4 0.08198415878204202 1108 5 -0.08689758002714854 1108 6 -0.05570801913339807 1108 7 -0.13455915145457453 1108 8 0.04413317917774151 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-0.762152958641849 2316 84-85 -1.0106195410507475 2316 86-87 -1.0015743549752116 2316 88-89 -0.959512835181723 2316 90-91 -0.9069842377397173 2316 92-93 -0.8647343794052595 2316 94-95 -0.5823012032731611 2316 96-97 -0.6356270444008665 2316 98-99 -0.6689555479867408 2316 100-101 -0.6992809148929418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 1.0 5 10.0 6 21.0 7 40.0 8 98.0 9 211.0 10 380.0 11 645.0 12 1018.0 13 1478.0 14 1854.0 15 2262.0 16 2918.0 17 4260.0 18 4449.0 19 2685.0 20 1782.0 21 1761.0 22 1819.0 23 1867.0 24 2103.0 25 2183.0 26 2364.0 27 2684.0 28 3230.0 29 4174.0 30 4996.0 31 6103.0 32 7917.0 33 10428.0 34 14656.0 35 25599.0 36 40635.0 37 80883.0 38 128094.0 39 42003.0 40 49.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.84051759036854 20.632463240435644 15.312784452660944 22.21423471653487 2 16.015228301877535 22.90966536002865 39.43021424611565 21.644892091978157 3 18.12385933237828 27.641933394823088 32.06576132817866 22.168445944619975 4 13.307539818840224 20.50240924837339 37.44492095113789 28.745129981648493 5 13.188867247867107 36.94236892327467 34.8092292143982 15.059534614460018 6 27.017234866139106 37.857097301195594 19.63538421535488 15.490283617310418 7 23.312450019869402 34.62697038232653 24.454082058175647 17.606497539628418 8 23.247199886180216 33.817476242573505 22.94130922185536 19.994014649390916 9 24.96210095618429 17.35530905505051 23.12479456019938 34.557795428565825 10-11 22.906504538692026 27.247115560336212 29.330093717459217 20.51628618351255 12-13 24.470885194106884 26.19191879547272 29.27241194911471 20.06478406130569 14-15 21.857472437303986 27.31236577405519 28.600934844227382 22.22922694441344 16-17 19.732591198887288 29.657331112490283 29.592815458245127 21.017262230377305 18-19 19.430704150379665 27.432931488502106 33.22204819439658 19.914316166721655 20-21 20.53993259121527 26.57691911436435 33.26947324008615 19.61367505433423 22-23 20.2808944664943 26.860119412650675 32.97339462594012 19.885591494914905 24-25 20.032772247597276 26.98080762985022 33.331043854958274 19.65537626759423 26-27 19.79573714434297 27.149273538552865 33.37012861176321 19.684860705340956 28-29 19.800537476802763 27.117556798738118 33.37544508647832 19.706460637980793 30-31 20.029092728780213 27.237270091399246 33.115914654787545 19.617722525032992 32-33 20.006770314623388 27.384696145336086 32.787210973796924 19.821322566243605 34-35 20.10723235762764 27.39294857090988 32.73494030584896 19.76487876561352 36-37 20.05875246079592 27.389072532917936 32.712855013890845 19.839319992395296 38-39 19.87577944473608 27.48048628520686 32.70822396985738 19.935510300199674 40-41 20.130524797503817 27.426029406714385 32.49640633662201 19.947039459159793 42-43 20.218710598486002 27.433879046857445 32.19726145679509 20.150148897861463 44-45 20.105749370802283 27.668387046131354 32.01868214354048 20.207181439525883 46-47 20.312047725812068 27.714381031344594 31.584376272500258 20.389194970343077 48-49 20.362579784233017 27.832984187881138 31.323866340252465 20.48056968763338 50-51 20.38674195779837 28.243986439746653 30.89826866374595 20.47100293870903 52-53 20.4215747359332 28.41729177603014 30.711226457212103 20.449907030824555 54-55 20.434453051792907 28.29120693123225 30.61237005166044 20.6619699653144 56-57 20.34737110645584 28.600654463746928 30.298139144683585 20.75383528511365 58-59 20.253397439042338 28.488078300544572 30.66489231222097 20.59363194819212 60-61 20.393252087564882 28.479070383562448 30.503100683916674 20.624576844955993 62-63 20.204649382764508 28.734446450650598 30.331629148430906 20.729275018153988 64-65 20.253254606246 28.53811943000542 30.322725455227857 20.885900508520727 66-67 20.28206364217315 28.64314827966446 30.09872718106852 20.97606089709387 68-69 20.22014225595524 28.6171440626721 30.074047180699896 21.088666500672765 70-71 20.287026997856067 28.494193719306683 30.066574760463325 21.15220452237393 72-73 20.343396703165954 28.516424680503444 29.822745186546246 21.31743342978435 74-75 20.318786183887745 28.635683446178 29.61816799136493 21.427362378569327 76-77 20.329075265388223 28.780410025572937 29.45769426540662 21.432820443632217 78-79 20.246577123422252 28.788835835613614 29.450904733461464 21.51368230750267 80-81 20.35755245227012 28.73568716920265 29.359288084242547 21.54747229428468 82-83 20.190741376772483 28.74000294391836 29.379446543349196 21.689809135959962 84-85 20.394010607034396 28.693328165552163 29.231897638683783 21.680763588729658 86-87 20.340236595671612 28.57423204528311 29.25568645247542 21.829844906569853 88-89 20.38693962255357 28.51931938463501 29.06720175243861 22.026539240372813 90-91 20.310085315776305 28.42219780111955 29.154912648223284 22.112804234880855 92-93 20.45027498270626 28.296235606948894 29.223596999475056 22.029892410869788 94-95 20.38208123396343 28.25612885184295 29.18214108746952 22.1796488267241 96-97 20.36540074865943 28.171990521559525 29.158224215158633 22.30438451462241 98-99 20.26323277617242 28.128449548890995 28.98565478263856 22.62266289229803 100-101 20.150665139246282 28.093210256611528 29.0356646118447 22.720459992297485 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2270.0 1 1461.0 2 672.0 3 637.0 4 509.0 5 492.0 6 726.0 7 1401.5 8 2114.5 9 2179.0 10 1572.5 11 991.0 12 886.5 13 905.0 14 962.5 15 980.0 16 947.5 17 1006.0 18 1084.5 19 1136.0 20 1202.0 21 1322.0 22 1510.5 23 1751.0 24 1971.5 25 2265.5 26 2726.0 27 3431.0 28 4348.0 29 5284.0 30 6410.0 31 7651.5 32 8903.0 33 10108.0 34 10950.5 35 11499.0 36 12179.0 37 12715.0 38 12854.0 39 13200.5 40 13442.5 41 13416.0 42 13531.5 43 13889.5 44 14238.5 45 14405.5 46 14702.5 47 14601.0 48 14259.5 49 14160.0 50 13666.0 51 12890.5 52 11819.5 53 10644.0 54 9709.0 55 8797.0 56 7765.5 57 6728.5 58 5971.0 59 5322.0 60 4522.0 61 3639.0 62 2862.5 63 2151.0 64 1623.5 65 1223.0 66 916.0 67 731.5 68 567.0 69 406.5 70 275.0 71 203.0 72 147.5 73 108.0 74 81.0 75 56.5 76 44.0 77 29.0 78 13.0 79 5.5 80 4.5 81 4.0 82 1.5 83 1.0 84 1.5 85 1.5 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019869401612119843 2 0.0 3 0.0014718075268236922 4 0.016189882795060614 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.226506272353077E-4 12-13 0.0 14-15 1.226506272353077E-4 16-17 0.002207711290235538 18-19 1.226506272353077E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.453012544706154E-4 28-29 0.0040474706987651534 30-31 0.0 32-33 0.0 34-35 0.010425303315001152 36-37 0.004783374462176999 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.906025089412308E-4 60-61 0.0024530125447061535 62-63 0.009321447669883385 64-65 0.002207711290235538 66-67 0.005764579480059461 68-69 0.0040474706987651534 70-71 0.0 72-73 0.006991085752412539 74-75 0.005396627598353538 76-77 0.001103855645117769 78-79 1.226506272353077E-4 80-81 0.007236387006883152 82-83 0.010302652687765847 84-85 0.001962410035764923 86-87 0.001103855645117769 88-89 3.6795188170592305E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.359037634118461E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 407662.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.4461967941342 #Duplication Level Percentage of deduplicated Percentage of total 1 73.00522668648199 47.77914432723877 2 17.98694579617225 23.543543886034275 3 5.463086023721582 10.726146090353 4 1.8543267036663418 4.854345214750622 5 0.7317145427273746 2.3943966980232836 6 0.3232329212772183 1.269261922575103 7 0.1756705319647576 0.8047877744127037 8 0.10965726737395608 0.5741320880370336 9 0.08575348122078998 0.505101528698212 >10 0.2455975204507657 2.5346787440828256 >50 0.008642721480384752 0.37602314796565506 >100 0.006763868975057442 0.8270541825585688 >500 0.0015030819944572096 0.6399033550453156 >1k 0.001878852493071512 3.171481040224592 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4148 1.0175096035441125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3304 0.8104753447709131 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2179 0.5345114334914709 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1663 0.40793598618463334 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1602 0.3929726096619258 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.18422124210743215 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 678 0.16631425053107723 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.15723810411566447 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.13050026737836737 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 472 0.11578219211013044 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.226506272353077E-4 0.0 22-23 0.0 0.0 0.0 0.001103855645117769 0.0 24-25 0.0 0.0 0.0 0.0018397594085296154 0.0 26-27 0.0 0.0 0.0 0.005396627598353538 0.0 28-29 0.0 0.0 0.0 0.013736870250354461 0.0 30-31 0.0 0.0 0.0 0.027351089873473615 0.0 32-33 0.0 0.0 0.0 0.0639009767895953 0.0 34-35 0.0 0.0 0.0 0.122773277862543 0.0 36-37 0.0 0.0 0.0 0.19440124416796267 0.0 38-39 4.906025089412308E-4 0.0 0.0 0.2839362020497373 0.0 40-41 4.906025089412308E-4 0.0 0.0 0.3740844130676884 0.0 42-43 4.906025089412308E-4 0.0 0.0 0.4649685278490514 0.0 44-45 4.906025089412308E-4 0.0 0.0 0.5638249334007094 0.0 46-47 4.906025089412308E-4 0.0 0.0 0.6728613410128979 0.0 48-49 4.906025089412308E-4 0.0 0.0 0.7701232884104969 0.0 50-51 4.906025089412308E-4 0.0 0.0 0.8714327065068611 0.0 52-53 4.906025089412308E-4 0.0 0.0 0.9720062208398135 0.0 54-55 4.906025089412308E-4 0.0 0.0 1.091467931767003 0.0 56-57 4.906025089412308E-4 0.0 0.0 1.2359503706501955 0.0 58-59 4.906025089412308E-4 0.0 0.0 1.3631390710932094 0.0 60-61 4.906025089412308E-4 0.0 0.0 1.487629457737047 0.0 62-63 4.906025089412308E-4 0.0 0.0 1.6193562313877674 0.0 64-65 4.906025089412308E-4 0.0 0.0 1.7762263836217258 0.0 66-67 4.906025089412308E-4 0.0 0.0 1.9713635315531004 0.0 68-69 4.906025089412308E-4 0.0 0.0 2.206239482708715 0.0 70-71 4.906025089412308E-4 0.0 0.0 2.4414833857460345 0.0 72-73 4.906025089412308E-4 0.0 0.0 2.6849448808081204 0.0 74-75 4.906025089412308E-4 0.0 0.0 2.937850474167325 0.0 76-77 4.906025089412308E-4 0.0 0.0 3.221418724335356 0.0 78-79 4.906025089412308E-4 0.0 0.0 3.5241204723520956 0.0 80-81 4.906025089412308E-4 0.0 0.0 3.830379088558659 0.0 82-83 4.906025089412308E-4 0.0 0.0 4.155771202613931 0.0 84-85 4.906025089412308E-4 0.0 0.0 4.521392722402382 0.0 86-87 4.906025089412308E-4 0.0 0.0 4.934480034930898 0.0 88-89 4.906025089412308E-4 0.0 0.0 5.435017244678189 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1100 0.0 62.615253 1 GTATCAA 2235 0.0 46.544827 1 GTGGTAT 435 0.0 43.67929 1 TGGTATC 455 0.0 39.671352 2 TATCAAC 2785 0.0 37.352848 2 ATCAACG 2855 0.0 36.10426 3 TCAACGC 2860 0.0 36.04114 4 CAACGCA 2960 0.0 34.819263 5 AACGCAG 3070 0.0 33.881084 6 ACGCAGA 3440 0.0 30.236898 7 CGCAGAG 3425 0.0 30.23065 8 GCAGAGT 3530 0.0 28.524149 9 CGTCAGA 120 5.3694257E-6 27.70905 2 GAGTATA 95 0.0012250122 25.000647 1 GCGTCAG 100 0.0016515732 23.750614 1 CAGAGTA 3495 0.0 23.175829 10-11 GAGTACT 2815 0.0 22.698751 12-13 CCGCCAT 110 0.0028758317 21.588818 9 GTAGGAG 155 3.8976927E-5 21.452168 3 AGAGTAC 3405 0.0 20.858458 10-11 >>END_MODULE