FastQCFastQC Report
Fri 10 Feb 2017
SRR2031742_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031742_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655932
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29050.4428812742784313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24130.36787349908222194No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21030.32061250251550466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12520.19087344419848398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.18401297695492827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.1208966783142155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.055.399221
CGGAGAT1550.042.9105031
GCGTCAG2700.042.229381
GTATCAA26000.041.295461
CGTCAGA3200.035.631042
ATCAACG30700.034.9706883
TCAACGC30900.034.59064
CAACGCA31250.034.203195
TATCAAC31400.034.1936952
AACGCAG32400.033.130756
GTGGTAT6250.031.9254111
TGGTATC6450.030.198922
ACGCAGA35950.029.8591427
CGCAGAG37000.029.144618
GTAATAC1606.227492E-826.7212413
GCAGAGT39200.026.5394639
CAGAGTA38750.023.23119510-11
GAGTACT24600.022.78754612-13
GTACTTT26450.020.74548314-15
GTACATG21000.020.5868241
Produced by FastQC (version 0.11.5)