FastQCFastQC Report
Fri 10 Feb 2017
SRR2031742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655932
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.5566125756938219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31840.4854161711884768No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT25250.384948439777294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17030.2596305714616759No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16320.2488062786996213No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.2201447711043218No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT10800.16465121384533762TruSeq Adapter, Index 3 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.14163053487251728No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT6740.10275455382570144RNA PCR Primer, Index 3 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.057.781031
GCGTCAG1700.036.335281
GTATCAA27050.036.00981
GTGGTAT6550.035.5458451
CGTCAGA2300.035.112022
TGGTATC7000.033.2531472
ATCAACG31950.030.6288783
TCAACGC31950.030.6288784
TATCAAC32300.030.4440572
CGGAGAT1102.7242113E-630.2370511
CAACGCA32850.030.0743665
AACGCAG33800.029.0885566
ACGCAGA38050.025.465027
CGCAGAG38350.025.018118
GTACATG21750.022.9384541
GCAGAGT42000.022.730839
GAGTACT27550.021.89695512-13
CAGAGTA40200.021.68261510-11
TACATGG22450.021.5833362
ACATGGG21400.021.3104293
Produced by FastQC (version 0.11.5)