##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031742_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655932
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.746748138526556	34.0	31.0	34.0	31.0	34.0
2	32.90786240037077	34.0	31.0	34.0	31.0	34.0
3	32.96343828323668	34.0	31.0	34.0	31.0	34.0
4	36.36524365330553	37.0	37.0	37.0	35.0	37.0
5	36.34093015739436	37.0	37.0	37.0	35.0	37.0
6	36.372431898428495	37.0	37.0	37.0	35.0	37.0
7	36.32596061786892	37.0	37.0	37.0	35.0	37.0
8	36.324474183299486	37.0	37.0	37.0	35.0	37.0
9	38.164017001762375	39.0	39.0	39.0	37.0	39.0
10-11	38.13581438319826	39.0	39.0	39.0	37.0	39.0
12-13	38.08549819188575	39.0	39.0	39.0	37.0	39.0
14-15	39.55418549483788	41.0	39.0	41.0	37.0	41.0
16-17	39.52545385802186	41.0	39.0	41.0	37.0	41.0
18-19	39.54937249592946	41.0	39.5	41.0	37.0	41.0
20-21	39.561661879585074	41.0	40.0	41.0	37.0	41.0
22-23	39.470644060664824	41.0	39.0	41.0	36.0	41.0
24-25	39.39594195739802	41.0	39.0	41.0	36.0	41.0
26-27	39.269300018904396	41.0	39.0	41.0	36.0	41.0
28-29	39.16134980455291	41.0	39.0	41.0	35.5	41.0
30-31	38.9705617350579	40.0	38.5	41.0	35.0	41.0
32-33	38.739969234615785	40.0	38.0	41.0	35.0	41.0
34-35	38.59296390479501	40.0	38.0	41.0	35.0	41.0
36-37	38.479681582847	40.0	38.0	41.0	35.0	41.0
38-39	38.519979967435646	40.0	38.0	41.0	35.0	41.0
40-41	38.43816279736314	40.0	38.0	41.0	35.0	41.0
42-43	38.314083624522056	40.0	38.0	41.0	35.0	41.0
44-45	38.09263841373801	40.0	38.0	41.0	34.0	41.0
46-47	37.827065915369275	40.0	38.0	41.0	33.0	41.0
48-49	37.53561573455785	40.0	38.0	41.0	33.0	41.0
50-51	37.34386338827805	40.0	38.0	41.0	33.0	41.0
52-53	37.39701828848112	40.0	38.0	41.0	33.0	41.0
54-55	37.34192111377399	40.0	38.0	41.0	33.0	41.0
56-57	37.173630803193014	40.0	37.0	41.0	33.0	41.0
58-59	37.00691611325564	40.0	37.0	41.0	32.0	41.0
60-61	36.763656141185365	40.0	36.5	41.0	31.5	41.0
62-63	36.50374962648567	39.5	36.0	41.0	31.0	41.0
64-65	36.195578200179284	39.0	35.5	41.0	31.0	41.0
66-67	35.84214141099992	39.0	35.0	41.0	30.5	41.0
68-69	35.44672389820896	38.5	35.0	40.5	29.5	41.0
70-71	35.10957766963649	37.5	35.0	40.0	29.0	41.0
72-73	34.729382009110694	37.0	35.0	39.5	29.0	41.0
74-75	34.34089966642884	36.5	35.0	39.0	29.0	41.0
76-77	33.38305037717324	35.5	33.5	38.5	27.0	39.0
78-79	33.54720306373221	36.0	34.0	37.5	28.0	39.0
80-81	33.2737151107127	35.0	34.0	37.0	28.0	39.0
82-83	32.95040796911875	35.0	34.0	37.0	27.5	39.0
84-85	32.61278227011337	35.0	34.0	36.0	26.5	37.5
86-87	32.32007891061878	35.0	34.0	36.0	26.0	37.0
88-89	32.05539827299171	35.0	34.0	36.0	26.0	37.0
90-91	31.82436517809773	35.0	34.0	35.0	25.5	36.0
92-93	31.627851667550907	35.0	34.0	35.0	25.0	36.0
94-95	31.431431154448937	35.0	34.0	35.0	24.0	36.0
96-97	31.25678728892629	35.0	33.0	35.0	23.5	36.0
98-99	31.05842450132026	35.0	33.0	35.0	20.0	35.0
100-101	30.406846288944585	34.5	32.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22110934558042317
1101	2	0.15033645754787983
1101	3	0.1960219828565215
1101	4	0.17804467990374917
1101	5	0.08961982726042095
1101	6	0.09289806064427353
1101	7	0.09914226599492793
1101	8	0.21255722570295177
1101	9	0.2138985459622873
1101	10-11	0.17761737688872614
1101	12-13	0.23449138856130247
1101	14-15	0.285274428705506
1101	16-17	0.09550091993585141
1101	18-19	0.15891708376317837
1101	20-21	0.2605905531038033
1101	22-23	0.090160537946808
1101	24-25	0.12554365648274057
1101	26-27	0.21586341282777965
1101	28-29	0.17932036147553987
1101	30-31	0.0564642617272213
1101	32-33	0.04863063779089316
1101	34-35	-0.28212951968213673
1101	36-37	0.12917794198285293
1101	38-39	0.06267232100982056
1101	40-41	-0.061929639386050894
1101	42-43	0.1974331561864986
1101	44-45	0.2999554441371046
1101	46-47	0.14468418182924125
1101	48-49	0.013121606438993183
1101	50-51	0.12764804779872208
1101	52-53	0.010664089894426354
1101	54-55	-0.03291160354925182
1101	56-57	-0.23750757012546586
1101	58-59	0.018375894360907807
1101	60-61	0.05999132024442133
1101	62-63	0.1628836112327008
1101	64-65	0.25659349717349045
1101	66-67	-0.05171570927778646
1101	68-69	-0.01729200601988623
1101	70-71	-0.0070167956263702536
1101	72-73	0.026428121174490116
1101	74-75	0.08919674056777893
1101	76-77	0.20640585336811057
1101	78-79	0.15444787814364958
1101	80-81	0.2024710402149381
1101	82-83	0.17252991858961764
1101	84-85	0.22827111852984672
1101	86-87	0.4767523751054199
1101	88-89	0.37868903716686475
1101	90-91	0.343787621792071
1101	92-93	-0.10565936073842153
1101	94-95	0.1956792066670161
1101	96-97	0.26626575490407944
1101	98-99	-0.22700259515344712
1101	100-101	-0.35294787498078506
1102	1	0.21721829499676204
1102	2	0.16565077590007604
1102	3	0.24455659178808986
1102	4	0.05476263228300127
1102	5	0.229858287975091
1102	6	0.20901557751144395
1102	7	0.2503899010215207
1102	8	0.17918343496293687
1102	9	0.19910541420203742
1102	10-11	0.2929709627238779
1102	12-13	0.26850238345652855
1102	14-15	0.3496249088397292
1102	16-17	0.27855700104105807
1102	18-19	0.19732246697812172
1102	20-21	0.3627770379501243
1102	22-23	0.3175442526506913
1102	24-25	0.2614519847588994
1102	26-27	0.27097354026861353
1102	28-29	0.20808914739635753
1102	30-31	0.11088720821635434
1102	32-33	0.3603144988554021
1102	34-35	0.27059421572641895
1102	36-37	0.2316339445530886
1102	38-39	0.14696889759925824
1102	40-41	0.3592319641551711
1102	42-43	0.41951576069154584
1102	44-45	0.2612305664017711
1102	46-47	0.5207524358541562
1102	48-49	0.5458349895111354
1102	50-51	0.571818611465595
1102	52-53	0.6806476689470102
1102	54-55	0.5562559258121169
1102	56-57	0.5697676235346236
1102	58-59	0.5388538867216042
1102	60-61	0.4881978672047609
1102	62-63	0.5411169314768713
1102	64-65	0.49167720830760686
1102	66-67	0.32883082268951114
1102	68-69	0.381085532979867
1102	70-71	0.4778973513768108
1102	72-73	0.47718848243532364
1102	74-75	0.5724206009183277
1102	76-77	0.3192302563238769
1102	78-79	0.22179161141034598
1102	80-81	0.04559030626998606
1102	82-83	0.18663586939817378
1102	84-85	0.32575978450636
1102	86-87	0.3554087132341479
1102	88-89	0.21915774812249822
1102	90-91	0.19521731514872087
1102	92-93	0.3502022035353072
1102	94-95	0.2433106634906359
1102	96-97	0.08484462899825118
1102	98-99	0.05896998900390926
1102	100-101	0.11034952265602271
1103	1	0.26218232377374306
1103	2	0.15825852456352862
1103	3	0.2076811380226644
1103	4	0.09839011561743405
1103	5	0.01698455026800616
1103	6	0.15236255130855625
1103	7	0.19557173720395582
1103	8	0.2102407909130406
1103	9	0.3733546386756643
1103	10-11	0.15562524296939984
1103	12-13	0.1982017432347689
1103	14-15	0.31318011971689685
1103	16-17	0.3285685514337686
1103	18-19	0.30054502654514437
1103	20-21	0.2186462875046118
1103	22-23	0.2211545754016626
1103	24-25	-0.034734735589594834
1103	26-27	-0.009509596158032707
1103	28-29	0.06958013809003916
1103	30-31	0.29568689140558035
1103	32-33	0.009552172936324155
1103	34-35	-0.12760722312250294
1103	36-37	-0.11657409452024581
1103	38-39	-0.1474374122152753
1103	40-41	-0.09172836849614896
1103	42-43	0.012527773099968442
1103	44-45	0.3151032480729441
1103	46-47	0.20631114485026103
1103	48-49	0.3864280196741703
1103	50-51	0.2936125524595852
1103	52-53	0.31983815142341143
1103	54-55	0.5496507512361788
1103	56-57	0.2549517697955963
1103	58-59	0.5417711859097736
1103	60-61	0.2909699614559713
1103	62-63	0.39288249150591525
1103	64-65	0.4052687204190235
1103	66-67	0.2887757108157132
1103	68-69	0.030390859360963418
1103	70-71	0.13287557063626565
1103	72-73	0.3340345652021455
1103	74-75	0.39735954884255165
1103	76-77	0.25868309772047837
1103	78-79	0.20411126027840965
1103	80-81	0.04239909776890016
1103	82-83	0.1656191048281812
1103	84-85	0.1304244931064531
1103	86-87	0.173285421042209
1103	88-89	0.3174119887666791
1103	90-91	0.2977633517139644
1103	92-93	0.36119322723011393
1103	94-95	0.4214441200281591
1103	96-97	0.47816355184162873
1103	98-99	0.5519143491121525
1103	100-101	0.5681113865593979
1104	1	0.15911970344746607
1104	2	-0.04266102148977069
1104	3	0.08921675459982481
1104	4	0.08835870412691094
1104	5	-0.008334631006690074
1104	6	-0.015612927463358517
1104	7	-0.005854445793310958
1104	8	0.0753360386585058
1104	9	8.498256246980418E-4
1104	10-11	0.02594518419952152
1104	12-13	0.08180190535858856
1104	14-15	-0.11421247325097994
1104	16-17	-0.02465239293749022
1104	18-19	0.10049309625838987
1104	20-21	0.1193961101816825
1104	22-23	0.17491762130297417
1104	24-25	0.07649107249633147
1104	26-27	0.0350871208345751
1104	28-29	0.022469235263010034
1104	30-31	-0.08474045685665743
1104	32-33	-0.052865498560286994
1104	34-35	-0.2227156434285078
1104	36-37	-0.1978449932941828
1104	38-39	-0.21760662895456306
1104	40-41	-0.1483513799461349
1104	42-43	-0.45605440341233816
1104	44-45	-0.23606075030581763
1104	46-47	-0.3684241373465156
1104	48-49	-0.39846659984149113
1104	50-51	-0.3327427013655253
1104	52-53	-0.20460074816114115
1104	54-55	0.0038444385398577197
1104	56-57	-0.1403580386956449
1104	58-59	-0.3252936129553632
1104	60-61	-0.222373589055735
1104	62-63	-0.09166508170312682
1104	64-65	-0.13036052266537723
1104	66-67	-0.15135880129290769
1104	68-69	-0.22425753459992137
1104	70-71	-0.48475286182902977
1104	72-73	-0.45665471436011984
1104	74-75	-0.300127531915372
1104	76-77	-0.171211015158228
1104	78-79	-0.27726349906904346
1104	80-81	-0.20245853724984642
1104	82-83	-0.23242021019867565
1104	84-85	-0.14990406095170528
1104	86-87	-0.2796733387105519
1104	88-89	-0.14976427022612882
1104	90-91	-0.3118475511130683
1104	92-93	-0.3439314054581004
1104	94-95	-0.19787521323762292
1104	96-97	-0.3772612632358836
1104	98-99	-0.42426104859784886
1104	100-101	-0.4046378788170344
1105	1	0.17098541828443103
1105	2	0.17261005703618082
1105	3	0.0067596834055336785
1105	4	0.0824740813215854
1105	5	0.016602663996202693
1105	6	0.08223098532845796
1105	7	0.06379633560742093
1105	8	0.038219503833119006
1105	9	0.047396227322558104
1105	10-11	-0.011388406385087535
1105	12-13	0.0818742572783222
1105	14-15	0.08012478316422289
1105	16-17	0.12257170507523796
1105	18-19	0.1741714678578532
1105	20-21	0.14922921842015313
1105	22-23	0.22809628231011203
1105	24-25	0.1086635594729799
1105	26-27	0.07685829582975146
1105	28-29	0.2414905549620201
1105	30-31	0.25140656224424873
1105	32-33	0.08393992301023445
1105	34-35	0.016998325315462637
1105	36-37	0.14048464205214373
1105	38-39	0.037783589065888634
1105	40-41	0.042042696843367366
1105	42-43	-0.022051412866325393
1105	44-45	-0.0030634514441558736
1105	46-47	-0.008099518399959038
1105	48-49	0.26928132607014277
1105	50-51	0.19150725039986582
1105	52-53	0.2629494158365375
1105	54-55	0.12720446169247168
1105	56-57	-0.04713259011374049
1105	58-59	0.0035579209053366867
1105	60-61	0.12998026006638952
1105	62-63	0.03896630938312029
1105	64-65	0.0849708491270178
1105	66-67	0.04963483173814609
1105	68-69	0.030686513248816993
1105	70-71	-0.004504935917395869
1105	72-73	-0.04883106010582594
1105	74-75	0.033655991140769004
1105	76-77	0.026514722831350923
1105	78-79	0.15705301646249836
1105	80-81	-0.005576904990540754
1105	82-83	0.006883764333451836
1105	84-85	0.30503275170504907
1105	86-87	-0.004205462962914908
1105	88-89	0.15246175717113175
1105	90-91	-0.0859769163455617
1105	92-93	-0.015562217728987804
1105	94-95	-0.08706824815948266
1105	96-97	-0.1146840878548332
1105	98-99	-0.11319602833931341
1105	100-101	-0.14515977733140772
1106	1	0.0375177958287054
1106	2	0.026463793958662052
1106	3	-0.0012691726106979218
1106	4	0.04952671050126156
1106	5	0.05620275972706423
1106	6	0.025843799587796923
1106	7	0.12396147134010249
1106	8	0.05741696012741926
1106	9	0.06772194017556643
1106	10-11	0.07539475182155542
1106	12-13	0.015079189696898254
1106	14-15	0.14871053066268303
1106	16-17	0.1515958201346166
1106	18-19	0.13272370955547785
1106	20-21	-0.029320231127407226
1106	22-23	0.03919165987718287
1106	24-25	0.03583260421576284
1106	26-27	-0.09230326139222456
1106	28-29	-0.18702755412557082
1106	30-31	0.15103589007686224
1106	32-33	0.022115167465820207
1106	34-35	0.07608506431211026
1106	36-37	0.17962887654716297
1106	38-39	0.0701825018368396
1106	40-41	-0.1116651644836466
1106	42-43	0.039016897939866624
1106	44-45	0.19533733035786582
1106	46-47	0.2619498285788211
1106	48-49	0.23759184734745986
1106	50-51	0.14862637833731895
1106	52-53	0.310179489002266
1106	54-55	0.3860609907650243
1106	56-57	0.4774810674888883
1106	58-59	0.2818327140583037
1106	60-61	0.38490213684710284
1106	62-63	0.4010583135770389
1106	64-65	0.42521000532786246
1106	66-67	0.42773326357550445
1106	68-69	0.35563864781904897
1106	70-71	0.38591457038488386
1106	72-73	0.3412422765704832
1106	74-75	0.34012580173043006
1106	76-77	0.2650483881836223
1106	78-79	0.21604430951289544
1106	80-81	0.1775126375527094
1106	82-83	-0.023147519143861928
1106	84-85	0.05621514178550768
1106	86-87	0.15988748714307377
1106	88-89	0.21140461496521823
1106	90-91	0.22355998562148116
1106	92-93	0.21470326445823673
1106	94-95	0.3448631219314606
1106	96-97	0.268005721367242
1106	98-99	0.28318956188702415
1106	100-101	0.3466788819806972
1107	1	0.2440519264815535
1107	2	0.17922350570920287
1107	3	0.24601397801185243
1107	4	0.20675422933540233
1107	5	0.2386387117331097
1107	6	0.20137414704090872
1107	7	0.2910949608432887
1107	8	0.18044387710240528
1107	9	0.234453594931054
1107	10-11	0.1700893425305381
1107	12-13	0.3307531260998573
1107	14-15	0.34861885280913185
1107	16-17	0.40699900921423193
1107	18-19	0.3959701175993118
1107	20-21	0.313514757665871
1107	22-23	0.4238546693515488
1107	24-25	0.3244675269295456
1107	26-27	0.2920695340976991
1107	28-29	0.40890974774757893
1107	30-31	0.34506849524594685
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2316	94-95	-0.7529517278202249
2316	96-97	-0.7069933280951197
2316	98-99	-0.6160896589980105
2316	100-101	-0.2905968590312895
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	3.0
5	8.0
6	10.0
7	51.0
8	104.0
9	196.0
10	448.0
11	724.0
12	1034.0
13	1497.0
14	1951.0
15	2524.0
16	3452.0
17	5126.0
18	5221.0
19	3119.0
20	2315.0
21	2309.0
22	2591.0
23	2650.0
24	2964.0
25	3365.0
26	3363.0
27	3370.0
28	4183.0
29	5443.0
30	6737.0
31	8700.0
32	11519.0
33	15193.0
34	21356.0
35	34411.0
36	56418.0
37	120614.0
38	229901.0
39	93005.0
40	54.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.21238247143175	21.85642530169351	15.552502447399963	22.37868977947478
2	15.209960788618332	23.123585981473692	40.66427617496935	21.002177054938624
3	17.6691740786252	28.05272578141913	32.39394318226863	21.884156957687043
4	12.433630882491972	20.949768375210812	39.03602001823721	27.580580724060006
5	12.412262246696304	38.15486971210431	35.93314550898569	13.499722532213704
6	26.35974460767275	39.09597336309252	20.16047395156815	14.383808077666588
7	23.020374063165082	34.84599013312356	24.846173078916717	17.287462724794644
8	22.37244104571815	35.46419445918175	23.41309769915174	18.750266795948363
9	24.249922248037905	17.755346590805143	23.454260502613074	34.540470658543875
10-11	22.231267875328538	27.926217961617972	30.47404304104694	19.36847112200655
12-13	23.54453170796036	26.696461444525838	29.66338710673294	20.095619740780858
14-15	21.07159218850168	28.47214758270884	28.253298177169683	22.2029620516198
16-17	19.707130034417176	30.940469338984407	29.22403180253765	20.128368824060768
18-19	19.287242103209028	29.2143533389945	31.87324581396519	19.625158743831285
20-21	20.503421086332118	28.077910515114375	31.630946500551886	19.787721898001625
22-23	20.227096711244457	28.49754242817853	31.39159242116561	19.883768439411405
24-25	20.157577309843095	28.681707859961094	31.768689437319725	19.392025392876093
26-27	19.842497957092853	28.828316522545155	31.888713395372665	19.440472124989327
28-29	19.723932787612576	28.667339542698407	31.85056663378519	19.758161035903832
30-31	20.0720501515401	28.601135483556224	31.824716586475425	19.50209777842825
32-33	20.017593327341846	28.8073202877439	31.51400948728562	19.661076897628636
34-35	20.376548367619524	28.8039832157255	31.40830553234571	19.411162884309267
36-37	19.883351654131783	28.71926184207116	31.42210052401041	19.975285979786644
38-39	19.67170377417171	28.61638096631968	31.730270820755813	19.981644438752795
40-41	19.96342608685047	28.42306824487904	31.673481397461934	19.94002427080856
42-43	20.072355061195367	28.401190977113483	31.409429635998855	20.1170243256923
44-45	20.053450662568682	28.762585146021237	31.16687400523225	20.017090186177835
46-47	20.22877371434844	28.57925821579066	30.672539226627148	20.51942884323375
48-49	20.18555277071404	28.580325399584105	30.817142630638543	20.416979199063316
50-51	20.07532793033424	28.999728630406814	30.437835019483728	20.487108419775222
52-53	20.301647121957764	28.842852612770837	30.581599921943127	20.273900343328272
54-55	20.276644530225692	29.078547776293885	30.114707012312252	20.53010068116817
56-57	20.45311099321271	29.256005195660528	29.677390339242482	20.613493471884283
58-59	20.014696678992667	29.27825432724961	30.30633580844312	20.400713185314604
60-61	20.408541298544485	29.554563901945208	29.83394291063378	20.202951888876523
62-63	19.98543913214178	29.811737889131567	29.861747990257175	20.341074988469472
64-65	20.155949371720244	29.663997633850574	29.64372070899106	20.53633228543812
66-67	20.182160942882604	29.669151026027457	29.618914977584353	20.529773053505586
68-69	20.096079668980995	29.481685098961147	29.77684574502145	20.64538948703641
70-71	20.11004206235107	29.187216338303877	29.876465664833653	20.826275934511404
72-73	20.24441617071168	29.3013533529657	29.64279806278344	20.811432413539173
74-75	20.031300694012465	29.39810579726054	29.48104577554978	21.08954773317721
76-77	20.05814684746972	29.314076065040922	29.620665761584203	21.007111325905154
78-79	20.123320992086814	29.350899218743447	29.41462548242535	21.11115430674439
80-81	20.094986163732973	29.334944388116817	29.41765705878317	21.15241238936704
82-83	20.137101594232952	29.286025761438346	29.381624849054074	21.19524779527463
84-85	20.188101920810585	29.066000326258802	29.49310968851432	21.252788064416293
86-87	20.12724025402314	28.993352131228516	29.577108340975215	21.302299273773126
88-89	20.194609180857714	29.029012242159986	29.25197810742	21.524400469562302
90-91	20.263990779532023	28.72652957928566	29.415244263124833	21.59423537805748
92-93	20.218559240896923	28.89354384295933	29.371108590524624	21.516788325619117
94-95	20.272147112810472	28.76159418964161	29.39885536915412	21.567403328393798
96-97	20.27649207539806	28.630101900806793	29.476149966764847	21.6172560570303
98-99	20.214305914565774	28.69735635504622	29.180867209457084	21.90747052093092
100-101	19.928528849921257	28.960756423332402	29.14637102491276	21.964343701833585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2345.0
1	1386.5
2	421.0
3	375.0
4	278.0
5	266.0
6	417.5
7	775.0
8	1187.5
9	1287.0
10	978.5
11	675.5
12	624.5
13	656.0
14	725.0
15	780.0
16	806.5
17	859.0
18	937.0
19	1054.5
20	1141.5
21	1230.0
22	1350.0
23	1545.5
24	1832.0
25	2217.0
26	2811.5
27	3700.0
28	5205.5
29	7088.5
30	9680.5
31	13328.5
32	17720.5
33	22628.5
34	27194.0
35	30394.5
36	32766.0
37	33786.0
38	32320.5
39	30736.0
40	28572.0
41	25705.5
42	23891.5
43	22859.5
44	21993.5
45	21124.0
46	20700.0
47	20129.0
48	19798.0
49	19404.5
50	18222.5
51	16807.5
52	15286.0
53	13810.5
54	12339.0
55	10908.5
56	9597.5
57	7963.5
58	6678.0
59	5812.5
60	4763.5
61	3796.5
62	2914.0
63	2167.5
64	1580.0
65	1228.0
66	998.5
67	705.0
68	509.5
69	366.5
70	250.5
71	193.0
72	163.0
73	126.0
74	78.5
75	51.5
76	33.0
77	20.5
78	14.0
79	9.0
80	9.0
81	4.5
82	1.0
83	2.0
84	2.0
85	0.5
86	1.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02042894690303263
2	0.0
3	0.0015245482763457188
4	0.019819127592494344
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.622741381728594E-5
14-15	2.2868224145185782E-4
16-17	0.001448320862528433
18-19	1.5245482763457188E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.098193105382875E-4
28-29	0.006250647933017447
30-31	0.0
32-33	2.2868224145185782E-4
34-35	0.01219638621076575
36-37	0.004421190001402585
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.2868224145185782E-4
60-61	0.0019819127592494343
62-63	0.009833336382429886
64-65	0.0021343675868840065
66-67	0.004344962587585299
68-69	0.0027441868974222936
70-71	1.5245482763457188E-4
72-73	0.006479330174469305
74-75	0.005488373794844587
76-77	9.909563796247172E-4
78-79	2.2868224145185782E-4
80-81	0.0071653768988248785
82-83	0.010366928279150887
84-85	0.0016770031039802908
86-87	0.0011434112072592892
88-89	3.0490965526914376E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	7.622741381728594E-5
100-101	4.5736448290371564E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	655932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.84929898945759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.64912812687869	30.60365991298734
2	19.269330820757745	18.825866051799302
3	8.066720836425223	11.821609740090695
4	3.7777270105242753	7.381572649506005
5	2.0159695352211506	4.923934928982792
6	1.144428108249631	3.3542706499095334
7	0.7347811207816716	2.5125479862610836
8	0.4977266794773826	1.945087950465647
9	0.32764038289478015	1.4404502722542734
>10	1.452224407133145	11.726299370110057
>50	0.04455544346568223	1.4798677881507902
>100	0.016316055014566257	1.2378455604705323
>500	0.0012550811549588987	0.41430120727315134
>1k	0.002196392021178073	2.332685931738842
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3651	0.5566125756938219	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3184	0.4854161711884768	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	2525	0.384948439777294	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1703	0.2596305714616759	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1632	0.2488062786996213	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1444	0.2201447711043218	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1080	0.16465121384533762	TruSeq Adapter, Index 3 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	929	0.14163053487251728	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	674	0.10275455382570144	RNA PCR Primer, Index 3 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	2.2868224145185782E-4	0.0
16-17	0.0	0.0	0.0	3.0490965526914376E-4	0.0
18-19	0.0	0.0	0.0	3.0490965526914376E-4	0.0
20-21	0.0	0.0	0.0	8.385015519901454E-4	0.0
22-23	0.0	0.0	0.0	0.0015245482763457188	0.0
24-25	0.0	0.0	0.0	0.0028966417250568654	0.0
26-27	0.0	0.0	0.0	0.005031009311940872	0.0
28-29	0.0	0.0	0.0	0.008994834830439741	0.0
30-31	0.0	0.0	0.0	0.017151168108889336	0.0
32-33	0.0	0.0	0.0	0.040400529323161544	0.0
34-35	0.0	0.0	0.0	0.07813309916271809	0.0
36-37	0.0	0.0	0.0	0.1258514602123391	0.0
38-39	0.0	0.0	0.0	0.1783721483324491	0.0
40-41	0.0	0.0	0.0	0.2282248769689541	0.0
42-43	0.0	0.0	0.0	0.2882158516431581	0.0
44-45	0.0	0.0	0.0	0.3642908106328095	0.0
46-47	0.0	0.0	0.0	0.44402468548569063	0.0
48-49	0.0	0.0	0.0	0.5223864668898606	0.0
50-51	0.0	0.0	0.0	0.5979278339827909	0.0
52-53	0.0	0.0	0.0	0.6785764378014794	0.0
54-55	0.0	0.0	0.0	0.7771384838672302	0.0
56-57	0.0	0.0	0.0	0.8876682339022948	0.0
58-59	0.0	0.0	0.0	0.9844770494502479	0.0
60-61	0.0	0.0	0.0	1.0798375441356725	0.0
62-63	0.0	0.0	0.0	1.1954745308964954	0.0
64-65	0.0	0.0	0.0	1.3349706981821288	0.0
66-67	0.0	0.0	0.0	1.5035857375459651	0.0
68-69	0.0	0.0	0.0	1.7030728795058026	0.0
70-71	0.0	0.0	0.0	1.9000445168096691	0.0
72-73	0.0	0.0	0.0	2.1048675777367167	0.0
74-75	0.0	0.0	0.0	2.3225730715988853	0.0
76-77	0.0	0.0	0.0	2.565814749089845	0.0
78-79	0.0	0.0	0.0	2.833068061933249	0.0
80-81	0.0	0.0	0.0	3.0975771878792315	0.0
82-83	0.0	0.0	0.0	3.384497173487496	0.0
84-85	0.0	0.0	0.0	3.706405542037894	0.0
86-87	0.0	0.0	0.0	4.078852685949153	0.0
88-89	0.0	0.0	0.0	4.504582792118695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1620	0.0	57.78103	1
GCGTCAG	170	0.0	36.33528	1
GTATCAA	2705	0.0	36.0098	1
GTGGTAT	655	0.0	35.545845	1
CGTCAGA	230	0.0	35.11202	2
TGGTATC	700	0.0	33.253147	2
ATCAACG	3195	0.0	30.628878	3
TCAACGC	3195	0.0	30.628878	4
TATCAAC	3230	0.0	30.444057	2
CGGAGAT	110	2.7242113E-6	30.237051	1
CAACGCA	3285	0.0	30.074366	5
AACGCAG	3380	0.0	29.088556	6
ACGCAGA	3805	0.0	25.46502	7
CGCAGAG	3835	0.0	25.01811	8
GTACATG	2175	0.0	22.938454	1
GCAGAGT	4200	0.0	22.73083	9
GAGTACT	2755	0.0	21.896955	12-13
CAGAGTA	4020	0.0	21.682615	10-11
TACATGG	2245	0.0	21.583336	2
ACATGGG	2140	0.0	21.310429	3
>>END_MODULE