FastQCFastQC Report
Fri 10 Feb 2017
SRR2031741_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031741_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976727
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.1333023454865075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12520.12818320779501335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.049.070461
CACCGTA500.001616068737.9982159
GTATCAA21400.034.182391
TCAACGC22950.031.870514
CGGGCTA300.003949554631.67005286-87
ATCAACG23150.031.5984083
CAACGCA24250.030.1619875
AACGCAG24500.029.851156
TATCAAC25150.028.8967462
ACGCAGA27600.026.5023787
CGCAGAG27750.026.3591238
GCGTCAG1151.2815211E-424.7827151
TGTATCG1351.3429455E-524.6284719
GTGGTAT7700.023.441661
CGGAGAT1252.2554962E-422.80011
GTAGACG1050.002195929222.6191463
GCAGAGT31900.021.7387929
ACGCATA600.004765962319.79073732-33
CAGAGTA31150.019.59478810-11
GAGTACT19350.019.27208512-13
Produced by FastQC (version 0.11.5)