FastQCFastQC Report
Fri 10 Feb 2017
SRR2031741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976727
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT39170.4010332467516512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.19718918387635437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.18439133964761903No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT15500.15869326843631845TruSeq Adapter, Index 3 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.054.0291331
AATACCG652.5900026E-643.8436245
CGGAGAT802.2613312E-741.5643581
AGCGTAA500.00161615537.9978078
GTATCAA23550.037.3158951
ATCAACG25850.033.9939773
TCAACGC25950.033.679944
CAACGCA27300.032.0128065
AACGCAG27500.031.9527036
ATACCGT902.4135465E-531.664846
TATCAAC28300.030.8831922
ACGCAGA29850.029.2780467
CGCAGAG30000.029.1316538
GTGGTAT6600.027.3497071
CGTATAC908.946921E-426.3887163
GCAGAGT34450.024.9550199
TTTACGC500.001654613923.7486338-39
GAGTACT24450.022.24309212-13
CAGAGTA35050.021.61430410-11
TTAGGCG1656.324277E-520.1503549
Produced by FastQC (version 0.11.5)