##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031741_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976727 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79626343901622 34.0 31.0 34.0 31.0 34.0 2 32.95350594383078 34.0 31.0 34.0 31.0 34.0 3 33.02002197133897 34.0 31.0 34.0 31.0 34.0 4 36.41010435874098 37.0 37.0 37.0 35.0 37.0 5 36.380207570795115 37.0 37.0 37.0 35.0 37.0 6 36.403505790256645 37.0 37.0 37.0 35.0 37.0 7 36.35570635397609 37.0 37.0 37.0 35.0 37.0 8 36.348261080117574 37.0 37.0 37.0 35.0 37.0 9 38.20734964836643 39.0 39.0 39.0 37.0 39.0 10-11 38.178914374231496 39.0 39.0 39.0 37.0 39.0 12-13 38.13542627571471 39.0 39.0 39.0 37.0 39.0 14-15 39.64979979052488 41.0 40.0 41.0 37.0 41.0 16-17 39.626316258278926 41.0 40.0 41.0 37.0 41.0 18-19 39.64472826081392 41.0 40.0 41.0 37.0 41.0 20-21 39.65133553183233 41.0 40.0 41.0 37.0 41.0 22-23 39.5707372684486 41.0 40.0 41.0 37.0 41.0 24-25 39.51523813716627 41.0 39.0 41.0 37.0 41.0 26-27 39.396730611521946 41.0 39.0 41.0 36.0 41.0 28-29 39.31452749847194 41.0 39.0 41.0 36.0 41.0 30-31 39.15920569411924 40.5 39.0 41.0 36.0 41.0 32-33 38.989126439629494 40.0 39.0 41.0 35.0 41.0 34-35 38.870336337584604 40.0 38.0 41.0 35.0 41.0 36-37 38.76904037668663 40.0 38.0 41.0 35.0 41.0 38-39 38.82468437956563 40.5 38.5 41.0 35.0 41.0 40-41 38.77618157376626 40.5 39.0 41.0 35.0 41.0 42-43 38.70746278130942 40.0 39.0 41.0 35.0 41.0 44-45 38.57243631024841 40.0 38.0 41.0 35.0 41.0 46-47 38.4238415647361 40.0 38.0 41.0 35.0 41.0 48-49 38.261417468750224 40.0 38.0 41.0 34.0 41.0 50-51 38.150642912502676 40.0 38.0 41.0 34.0 41.0 52-53 38.2302025028488 40.0 38.0 41.0 34.0 41.0 54-55 38.212989402361146 40.0 38.0 41.0 34.0 41.0 56-57 38.09343654880023 40.0 38.0 41.0 34.0 41.0 58-59 37.977368804179676 40.0 38.0 41.0 34.0 41.0 60-61 37.806324592235086 40.0 38.0 41.0 34.0 41.0 62-63 37.62103637966392 40.0 37.0 41.0 33.0 41.0 64-65 37.37722464926228 40.0 37.0 41.0 33.0 41.0 66-67 37.081226381578475 39.5 36.5 41.0 32.5 41.0 68-69 36.74788861165914 39.0 36.0 41.0 32.0 41.0 70-71 36.45945642948337 39.0 36.0 41.0 32.0 41.0 72-73 36.124536846017364 38.5 35.0 40.5 31.0 41.0 74-75 35.7618177853177 37.5 35.0 40.0 31.0 41.0 76-77 34.75700323631885 36.5 34.5 39.0 29.5 40.0 78-79 34.93561250994392 36.5 35.0 39.0 31.0 40.0 80-81 34.633585433800846 36.0 35.0 39.0 31.0 39.5 82-83 34.25340038721158 36.0 35.0 37.0 30.5 39.0 84-85 33.8524306177673 35.5 35.0 37.0 30.0 39.0 86-87 33.49673552589414 35.0 34.0 36.5 30.0 37.5 88-89 33.18868476042948 35.0 34.0 36.0 30.0 37.0 90-91 32.91045450775907 35.0 34.0 36.0 29.0 37.0 92-93 32.697992376580146 35.0 34.0 35.5 29.0 36.0 94-95 32.48417060243036 35.0 34.0 35.0 29.0 36.0 96-97 32.30448426223499 35.0 34.0 35.0 29.0 36.0 98-99 32.10234999134866 35.0 34.0 35.0 29.0 36.0 100-101 31.449971179254796 34.5 33.0 35.0 26.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12831341665729923 1101 2 0.1625782387789556 1101 3 0.1316618548998889 1101 4 0.06991297026136323 1101 5 0.0684720446071907 1101 6 0.1464085202294214 1101 7 0.15533744415098028 1101 8 0.08819490477640812 1101 9 0.00807865729375834 1101 10-11 0.16144320238097265 1101 12-13 0.22848105553740083 1101 14-15 0.2312365494931896 1101 16-17 0.1978307969266524 1101 18-19 0.21684352817054986 1101 20-21 0.1937949493075024 1101 22-23 0.00439723640941736 1101 24-25 0.05949651855821969 1101 26-27 0.007209309499735639 1101 28-29 0.19397377307500818 1101 30-31 0.05991714420644456 1101 32-33 0.24767798576817057 1101 34-35 0.01989491339570293 1101 36-37 0.11113626707791013 1101 38-39 0.15252870455462642 1101 40-41 0.02900220984692936 1101 42-43 -0.020676713822865622 1101 44-45 0.0553171090411837 1101 46-47 0.0684737541580418 1101 48-49 -0.04164785930804982 1101 50-51 0.1436666601357217 1101 52-53 0.052011713503887336 1101 54-55 0.07286652485031908 1101 56-57 -0.20756433318459955 1101 58-59 -0.14246067652149463 1101 60-61 0.10910233946705716 1101 62-63 0.14713295709433538 1101 64-65 0.04954095168287864 1101 66-67 -0.13544230738151697 1101 68-69 -0.032377725889780606 1101 70-71 0.03157105332618215 1101 72-73 -0.09915984548860735 1101 74-75 0.021070601106018216 1101 76-77 0.17339298511916468 1101 78-79 0.1960608713386307 1101 80-81 0.07728228671435033 1101 82-83 0.07899845916218595 1101 84-85 0.055159519127109036 1101 86-87 0.13140370531475298 1101 88-89 0.13734528077885244 1101 90-91 0.16049533251010217 1101 92-93 0.06076037708253068 1101 94-95 0.16607113475771484 1101 96-97 0.09249067911215292 1101 98-99 -0.3693004602929726 1101 100-101 -0.3632030764903469 1102 1 0.17948377798497006 1102 2 0.10695948710271352 1102 3 0.12459622033376405 1102 4 0.02513312396666123 1102 5 0.10285227562754784 1102 6 0.06700798938200592 1102 7 0.07150239527417313 1102 8 -0.006391191460494383 1102 9 -0.00891493369478269 1102 10-11 0.15150137363938398 1102 12-13 0.18429771896719416 1102 14-15 0.10537530967778252 1102 16-17 0.07374058985917031 1102 18-19 0.10084319523488716 1102 20-21 0.09764821716348848 1102 22-23 -0.026635141583533084 1102 24-25 -0.00971248180245965 1102 26-27 0.044290018652688445 1102 28-29 0.07323835519451194 1102 30-31 -0.1501421368374949 1102 32-33 0.14394771914219007 1102 34-35 -0.060268595006306214 1102 36-37 0.03229525310386805 1102 38-39 -0.13045273428232207 1102 40-41 0.09631763864031484 1102 42-43 0.11754245032366128 1102 44-45 0.061920332968163905 1102 46-47 -0.00767834194937933 1102 48-49 0.12769616495926073 1102 50-51 0.007218528737112706 1102 52-53 0.079640749192734 1102 54-55 0.09059766080829235 1102 56-57 0.16370979309049716 1102 58-59 -0.02600024499988507 1102 60-61 -0.03498481569020129 1102 62-63 -0.006944579921629668 1102 64-65 0.04752021718981325 1102 66-67 0.2266807123449226 1102 68-69 0.027784210316049496 1102 70-71 -0.03612355219130592 1102 72-73 -0.08372226630078927 1102 74-75 -0.0612708614063493 1102 76-77 -0.04031702792082115 1102 78-79 0.11867645087788503 1102 80-81 0.005948509466804808 1102 82-83 -0.06566439724081619 1102 84-85 0.08978251573301321 1102 86-87 0.13805317030563913 1102 88-89 0.112120780413548 1102 90-91 -0.15577273618561804 1102 92-93 -0.09131029775954858 1102 94-95 -0.020042211338157756 1102 96-97 -0.16208025005578008 1102 98-99 -0.041298721628969304 1102 100-101 -0.12350558553047364 1103 1 0.2138682127384115 1103 2 0.1702109569379857 1103 3 0.150857571464023 1103 4 0.17187241432607436 1103 5 0.07581995984984502 1103 6 0.11451059359465177 1103 7 0.0995160725401334 1103 8 0.14264922238862 1103 9 0.2070419746781269 1103 10-11 0.10086562206655714 1103 12-13 0.06340928520247502 1103 14-15 0.10142338020633446 1103 16-17 0.10495656589453262 1103 18-19 0.2124006026749896 1103 20-21 0.258559132492735 1103 22-23 0.06375016418339641 1103 24-25 -0.008058733207555235 1103 26-27 0.14585136919442476 1103 28-29 0.10944426749286862 1103 30-31 0.24344414352291466 1103 32-33 0.16038589189712837 1103 34-35 0.25206625083018963 1103 36-37 0.15274597316130212 1103 38-39 -0.00767293591017193 1103 40-41 0.04472333083121072 1103 42-43 0.08017199886795368 1103 44-45 0.1155244455643114 1103 46-47 -0.06706395374808238 1103 48-49 -0.03800238197835881 1103 50-51 -0.04743609675651328 1103 52-53 -0.11935420128308749 1103 54-55 -0.010723131107759798 1103 56-57 -0.13980971937734665 1103 58-59 0.12699136266738975 1103 60-61 -0.028571390311931566 1103 62-63 -0.11431612014421688 1103 64-65 0.06557535587517549 1103 66-67 -0.028071607905552298 1103 68-69 0.02401325155383205 1103 70-71 -0.013114524254142168 1103 72-73 0.0379157981659759 1103 74-75 0.04197513516844964 1103 76-77 -0.13026388432718505 1103 78-79 0.05206451681596036 1103 80-81 0.06996285176334283 1103 82-83 0.08711534227906981 1103 84-85 0.0487514535085154 1103 86-87 0.13131826443984096 1103 88-89 0.26957927485495503 1103 90-91 0.19025723727200727 1103 92-93 0.30628897129533783 1103 94-95 0.2568378242086169 1103 96-97 0.2816282958813545 1103 98-99 0.3547255137330012 1103 100-101 0.1450277277930887 1104 1 0.1557115887619247 1104 2 0.10593258306924014 1104 3 0.12422409190116923 1104 4 0.05654814943331132 1104 5 0.04196524643393218 1104 6 -0.008062530376307109 1104 7 0.07221682505126381 1104 8 0.11443834246994555 1104 9 0.05276890485260566 1104 10-11 0.04619115771966875 1104 12-13 0.10048786239288177 1104 14-15 0.19061970715640086 1104 16-17 0.010286300581867636 1104 18-19 0.09348229599079616 1104 20-21 0.1404557699503357 1104 22-23 0.04499816135457735 1104 24-25 0.08488909931728017 1104 26-27 0.099239326806277 1104 28-29 0.15704253041871397 1104 30-31 0.04013906158649405 1104 32-33 0.1886230157796831 1104 34-35 0.10736532151761935 1104 36-37 0.10546678158589629 1104 38-39 0.14264834545827654 1104 40-41 0.1503621917879272 1104 42-43 0.1883924371145227 1104 44-45 0.2528577273305572 1104 46-47 0.24095316284141433 1104 48-49 0.3993463699139639 1104 50-51 0.3496406019185798 1104 52-53 0.3460797357535057 1104 54-55 0.37015275428070993 1104 56-57 0.2079490461065845 1104 58-59 0.09325129393582898 1104 60-61 0.29515204803409034 1104 62-63 0.2336208280419143 1104 64-65 0.11838511752473124 1104 66-67 0.12792251344654204 1104 68-69 0.21758320414894428 1104 70-71 0.2666419870114325 1104 72-73 0.28732599960912353 1104 74-75 0.22613869860148839 1104 76-77 0.21167286495732185 1104 78-79 0.2858899474932173 1104 80-81 0.16376292248391877 1104 82-83 0.29038865520657 1104 84-85 0.18095611222648245 1104 86-87 0.20212033522671646 1104 88-89 0.29962308846203456 1104 90-91 0.26457047417488866 1104 92-93 0.14966913859370834 1104 94-95 0.22526357312846557 1104 96-97 0.25667857379112036 1104 98-99 0.2584322664430303 1104 100-101 0.33844712291115897 1105 1 0.06634944710037871 1105 2 0.06631406918064897 1105 3 -0.01574507986822482 1105 4 -0.06258283346814153 1105 5 -0.06085643714764899 1105 6 -0.0567944413028485 1105 7 0.018587886771292972 1105 8 0.023937357237834078 1105 9 -0.06396785807724115 1105 10-11 -0.007182202875291921 1105 12-13 -0.010865878310980293 1105 14-15 0.006805057920217905 1105 16-17 0.07389998365494677 1105 18-19 0.015978694066944854 1105 20-21 0.04017264814174837 1105 22-23 0.07431543787639328 1105 24-25 0.14239010573717792 1105 26-27 0.045806375559372725 1105 28-29 0.1317655000989859 1105 30-31 0.06835854587576051 1105 32-33 0.09128499641405341 1105 34-35 0.0658584460806182 1105 36-37 0.16612609255905397 1105 38-39 0.05014114161056682 1105 40-41 0.050778266962190344 1105 42-43 0.004097089952630029 1105 44-45 0.16313539328390192 1105 46-47 0.10746745400398794 1105 48-49 0.2572875526162548 1105 50-51 0.16797916157280213 1105 52-53 0.24942899524033635 1105 54-55 0.22873354331391482 1105 56-57 -0.010691704364838017 1105 58-59 0.1227910422249181 1105 60-61 0.14265956969860127 1105 62-63 0.040472958473934284 1105 64-65 0.08653854888689239 1105 66-67 -0.09411622842264222 1105 68-69 -0.11284696090391577 1105 70-71 -0.1606799296358119 1105 72-73 -0.25934672323342767 1105 74-75 -0.0992143429053911 1105 76-77 -0.264391556510148 1105 78-79 -0.11629398368884836 1105 80-81 -0.225132293313699 1105 82-83 -0.15859380130549283 1105 84-85 -0.10114868256979292 1105 86-87 -0.16767541450313672 1105 88-89 -0.2550519968188141 1105 90-91 -0.32863460241896547 1105 92-93 -0.18907723299771106 1105 94-95 -0.15601993006029602 1105 96-97 -0.0048714187931508945 1105 98-99 -0.08365515436761228 1105 100-101 0.004318364727378565 1106 1 0.1761314181107636 1106 2 0.04447703206868425 1106 3 0.011549003863805751 1106 4 0.06680707984624235 1106 5 0.08256046300710551 1106 6 0.1892430044554203 1106 7 0.10761891961472259 1106 8 0.12884642331918172 1106 9 0.10981176625137579 1106 10-11 0.1470356288716772 1106 12-13 0.14686438983787298 1106 14-15 0.2252133603184916 1106 16-17 0.19106843899212578 1106 18-19 0.1620400365307546 1106 20-21 0.1461848822434746 1106 22-23 0.013144297004672012 1106 24-25 0.13078692760178257 1106 26-27 0.15972538469322473 1106 28-29 0.14790905228736762 1106 30-31 0.15368242842028934 1106 32-33 0.18870958903211488 1106 34-35 0.1395311042034777 1106 36-37 0.03677727146319398 1106 38-39 0.007437475931801885 1106 40-41 0.15269139421910438 1106 42-43 -0.03596023685314975 1106 44-45 -0.007111564627550138 1106 46-47 -0.045224908153741694 1106 48-49 0.05381405899377256 1106 50-51 0.019659011466089282 1106 52-53 -0.03741112694895321 1106 54-55 -0.04173986433101362 1106 56-57 -0.0243817350133142 1106 58-59 0.012142341497650477 1106 60-61 0.03974758543755286 1106 62-63 0.015792339840068337 1106 64-65 0.11083669379448935 1106 66-67 0.0903758211813468 1106 68-69 0.13993909151205486 1106 70-71 0.0658677929702165 1106 72-73 0.05861538089045126 1106 74-75 -0.01578007022501282 1106 76-77 -0.030196888130667787 1106 78-79 0.010094301109305093 1106 80-81 -0.010948903167737 1106 82-83 -0.014466321864247789 1106 84-85 0.10996922807422749 1106 86-87 0.08636553104777533 1106 88-89 0.16929533231341765 1106 90-91 0.2792217240125652 1106 92-93 0.2138256477564653 1106 94-95 0.17056901120185586 1106 96-97 0.2589874060452573 1106 98-99 0.3028443449395155 1106 100-101 0.29135572956558775 1107 1 0.25285275051667355 1107 2 0.17876164058866806 1107 3 0.2502726158190711 1107 4 0.13588912899354 1107 5 0.0774374617689162 1107 6 0.06356721914800545 1107 7 0.15309420033577226 1107 8 0.19524328568041938 1107 9 0.15296892881091395 1107 10-11 0.09203717827366376 1107 12-13 0.2064824522741162 1107 14-15 0.22029317779494306 1107 16-17 0.25436915730286813 1107 18-19 0.34711643601313824 1107 20-21 0.35035417003724945 1107 22-23 0.3414612474623766 1107 24-25 0.19803496358514394 1107 26-27 0.22900626267881563 1107 28-29 0.29607311068320286 1107 30-31 0.37076362107412564 1107 32-33 0.2688429306998543 1107 34-35 0.2969869196477575 1107 36-37 0.4662202980237993 1107 38-39 0.3212073372037949 1107 40-41 0.30415707529764546 1107 42-43 0.23565924065923838 1107 44-45 0.3382621072423575 1107 46-47 0.38224445957055053 1107 48-49 0.43370215626163855 1107 50-51 0.4624521643062778 1107 52-53 0.3983192797657438 1107 54-55 0.4051848054734748 1107 56-57 0.48031035957867374 1107 58-59 0.31589495320420724 1107 60-61 0.3235029368592066 1107 62-63 0.3469547371173789 1107 64-65 0.39240958285065375 1107 66-67 0.45298442894059576 1107 68-69 0.4676834947129933 1107 70-71 0.48017522358293974 1107 72-73 0.40691762200400916 1107 74-75 0.4550672332170578 1107 76-77 0.2946528597756455 1107 78-79 0.4035140640308583 1107 80-81 0.5184704501265074 1107 82-83 0.49263432808660923 1107 84-85 0.5404915161885171 1107 86-87 0.5242132321475168 1107 88-89 0.5645078796765759 1107 90-91 0.4362971712444548 1107 92-93 0.3676653052654757 1107 94-95 0.3912730115848859 1107 96-97 0.4973599088813927 1107 98-99 0.6826599594536233 1107 100-101 0.5790643834743996 1108 1 0.00435933081573836 1108 2 -0.05634681680671605 1108 3 9.51065386921357E-4 1108 4 0.04848793431727216 1108 5 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-0.2903400133785965 2316 94-95 -0.3910862436668907 2316 96-97 -0.5063266431634865 2316 98-99 -0.5330225337168741 2316 100-101 -0.3982800392835948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 1.0 5 3.0 6 11.0 7 30.0 8 132.0 9 272.0 10 505.0 11 698.0 12 1194.0 13 1600.0 14 2034.0 15 2384.0 16 2908.0 17 3933.0 18 3920.0 19 2608.0 20 2165.0 21 2379.0 22 2608.0 23 2886.0 24 3489.0 25 4327.0 26 4171.0 27 4034.0 28 5310.0 29 6849.0 30 9025.0 31 11657.0 32 15403.0 33 20616.0 34 28578.0 35 43405.0 36 70610.0 37 152137.0 38 356077.0 39 208646.0 40 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.358890126814565 24.389889242061802 16.91997493200511 21.331245699118522 2 13.391971349210168 24.15884889022214 42.96174877934162 19.487430981226076 3 15.511689694537301 29.629830605652625 35.042975688916414 19.815504010893658 4 11.706288049071462 21.37946924585396 42.19582503443297 24.718417670641603 5 11.012186619188371 39.38142387791061 37.914688546543715 11.691700956357304 6 24.51688138036524 41.4154620482489 21.203570700922572 12.864085870463294 7 20.771413097006636 36.08019436341987 27.433049357701794 15.715343181871699 8 20.3926992905899 37.77043124639741 25.081624650490873 16.75524481252182 9 22.724261743557822 18.719765093009613 25.553301997385148 33.00267116604742 10-11 20.420342634123966 29.046038452914686 33.06384486146078 17.469774051500572 12-13 20.94024225807211 27.927506867323217 32.187090149038575 18.945160725566097 14-15 19.192517458819097 30.26198722877529 29.521043239308426 21.02445207309719 16-17 18.286310747851335 32.700085951685836 30.79205067207666 18.22155262838617 18-19 18.17249668791452 31.006136828547614 32.69396944485967 18.127397038678197 20-21 18.784368610676268 30.37481302349582 32.287169290907286 18.553649074920628 22-23 18.876205940861674 30.55695194255918 31.790101021063204 18.77674109551594 24-25 18.71654003626397 30.872802738124367 31.757901644983704 18.652755580627954 26-27 18.619199097389945 30.86350992041756 32.29457072271258 18.222720259479914 28-29 18.536870806528736 30.360859945098994 32.63589329832993 18.466375950042337 30-31 18.69174092413887 30.294437894782106 32.4510315334247 18.56278964765433 32-33 18.839569233257926 30.686320130518002 31.916299751157823 18.557810885066246 34-35 18.835237366771416 30.56759549093754 32.098092280344986 18.499074861946056 36-37 18.480636933936328 30.751864225605342 32.04077286396042 18.726725976497907 38-39 18.52692717617103 30.408855289144256 32.32689379939328 18.737323735291437 40-41 18.476350095779065 30.243507141708992 32.44903642471233 18.83110633779961 42-43 18.978588694691556 30.34998520569207 31.818819383512487 18.852606716103885 44-45 18.814059609286936 30.651092884705754 31.572384095043958 18.962463410963352 46-47 19.05092211027237 30.502381934767854 31.470922785998546 18.975773168961236 48-49 18.868015320555283 30.426618696933737 31.51730217348348 19.188063809027497 50-51 18.592708095506726 30.48236610639411 31.717716414105478 19.207209383993685 52-53 18.728774775346643 30.799752643266746 31.467748920629816 19.003723660756794 54-55 18.82209665546258 30.572462929764406 31.167306729516024 19.438133685256986 56-57 18.93758440178269 30.98291538986841 30.928294190700168 19.15120601764874 58-59 18.523679375628056 30.927010710788238 31.594137759278322 18.95517215430538 60-61 18.802615918194967 30.96765936752115 31.287968568027953 18.941756146255937 62-63 18.723422773093326 31.119660876376535 31.02003307274468 19.136883277785458 64-65 18.616389807089906 31.0952663488272 31.213573072959566 19.07477077112333 66-67 18.608776495125507 31.263825741771363 31.073125855269794 19.054271907833336 68-69 18.492967300185576 31.21935112305625 30.982939783707685 19.30474179305049 70-71 18.76858890680591 30.96721185594717 31.08034503058691 19.183854206660012 72-73 18.727192490262652 30.693123036659937 31.128385545721503 19.451298927355907 74-75 18.747353887219663 30.89866213561715 30.859651969936575 19.494332007226607 76-77 18.87220535439585 30.655469911022852 31.00562448583845 19.466700248742846 78-79 18.638741257738904 31.04233450494051 31.15516099275023 19.163763244570355 80-81 18.717225554737002 30.710131965537524 31.082985676130757 19.489656803594713 82-83 18.828132279504295 30.838885595246506 30.69410185444012 19.63888027080908 84-85 18.80103244883061 30.67357969176017 30.883571019763345 19.64181683964587 86-87 18.736066140240858 30.71262014154626 30.839627833164823 19.711685885048055 88-89 18.824262956711905 30.61869607928958 30.76075265927358 19.79628830472493 90-91 18.689510989252884 30.534939650485754 30.92276552199335 19.852783838268014 92-93 18.96952782097761 30.14864952028561 30.905053305580783 19.976769353155998 94-95 18.901596863811484 30.21402090860599 31.019875564000994 19.864506663581533 96-97 18.92561530786766 30.101005757497106 30.83309401352374 20.140284921111487 98-99 18.900368270765526 30.12714914198133 30.805332503350474 20.167150083902666 100-101 18.741335779621142 30.286735782487884 30.76589148556697 20.20603695232401 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 728.0 1 430.0 2 145.5 3 158.5 4 122.5 5 108.5 6 189.5 7 379.0 8 557.5 9 651.5 10 569.5 11 424.5 12 421.0 13 463.0 14 496.0 15 563.0 16 639.5 17 693.0 18 737.5 19 784.0 20 880.0 21 1025.0 22 1227.0 23 1469.0 24 1787.0 25 2313.5 26 3418.0 27 5746.5 28 10248.5 29 17405.5 30 26952.0 31 36530.0 32 45970.5 33 54292.5 34 60463.5 35 65275.5 36 69135.0 37 69552.0 38 64901.0 39 58917.0 40 52341.0 41 45181.5 42 38961.5 43 33779.0 44 28728.5 45 23874.5 46 19996.5 47 17112.0 48 14965.0 49 13200.5 50 11486.5 51 10323.0 52 9334.5 53 8296.5 54 7258.5 55 6233.5 56 5439.5 57 4655.5 58 3956.0 59 3401.0 60 2828.0 61 2279.5 62 1770.0 63 1322.0 64 965.5 65 726.0 66 536.5 67 398.5 68 285.0 69 195.5 70 133.5 71 92.5 72 77.5 73 55.0 74 39.0 75 35.0 76 23.0 77 13.0 78 7.5 79 4.0 80 3.5 81 2.5 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018736043950868565 2 0.0 3 0.0012285930459585944 4 0.01965748873533751 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0015869326843631845 18-19 1.0238275382988286E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.0953101531953143E-4 28-29 0.006142965229792971 30-31 1.535741307448243E-4 32-33 3.5833963840459004E-4 34-35 0.011876399444266413 36-37 0.004914372183834378 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.535741307448243E-4 60-61 0.0014845499305333016 62-63 0.009419213352349224 64-65 0.0029179084841516618 66-67 0.006296539360537796 68-69 0.0040441187762803735 70-71 2.0476550765976571E-4 72-73 0.007678706537241215 74-75 0.0059893910990481474 76-77 0.0015869326843631845 78-79 6.142965229792972E-4 80-81 0.007217984145006742 82-83 0.010033509875328521 84-85 0.0020476550765976573 86-87 0.0014845499305333016 88-89 3.5833963840459004E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 5.119137691494143E-5 98-99 0.0 100-101 9.214447844689457E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 976727.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.408561737879175 #Duplication Level Percentage of deduplicated Percentage of total 1 56.645490283225854 18.3579886901636 2 17.030820952578964 11.038888245768431 3 7.725375959135553 7.5110497115991635 4 4.410017750245547 5.7168933009590335 5 2.82041524144246 4.570280073937169 6 1.9560044811722805 3.8034775192584114 7 1.4861915699805086 3.371573187502023 8 1.1390717822751726 2.95325425437928 9 0.9292026411371664 2.7102709046064826 >10 5.596993860657165 31.90352293335095 >50 0.2103730567880159 4.514048510875517 >100 0.04717374752745925 2.2767220355450513 >500 0.0015937076855135868 0.3222945892489416 >1k 0.0012749661484108695 0.9497360428060156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 3917 0.4010332467516512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1926 0.19718918387635437 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1801 0.18439133964761903 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1550 0.15869326843631845 TruSeq Adapter, Index 3 (95% over 21bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0238275382988286E-4 0.0 10-11 0.0 0.0 0.0 1.0238275382988286E-4 0.0 12-13 0.0 0.0 0.0 1.0238275382988286E-4 0.0 14-15 0.0 0.0 0.0 1.0238275382988286E-4 0.0 16-17 0.0 0.0 0.0 1.0238275382988286E-4 0.0 18-19 0.0 0.0 0.0 1.0238275382988286E-4 0.0 20-21 0.0 0.0 0.0 4.6072239223447287E-4 0.0 22-23 0.0 1.0238275382988286E-4 0.0 8.190620306390629E-4 0.0 24-25 0.0 1.0238275382988286E-4 0.0 0.00179169819202295 0.0 26-27 0.0 1.0238275382988286E-4 0.0 0.0026619515995769546 0.0 28-29 0.0 1.0238275382988286E-4 0.0 0.004146501530110256 0.0 30-31 0.0 1.0238275382988286E-4 0.0 0.008037046175645806 0.0 32-33 0.0 1.0238275382988286E-4 0.0 0.021909909319594932 0.0 34-35 0.0 1.0238275382988286E-4 0.0 0.052931883730049444 0.0 36-37 0.0 1.0238275382988286E-4 0.0 0.09593264033860024 0.0 38-39 0.0 1.0238275382988286E-4 0.0 0.1341725988940615 0.0 40-41 0.0 1.0238275382988286E-4 0.0 0.16954584034228604 0.0 42-43 0.0 1.0238275382988286E-4 0.0 0.22211938443393087 0.0 44-45 0.0 1.0238275382988286E-4 0.0 0.29348016385335923 0.0 46-47 0.0 1.0238275382988286E-4 0.0 0.36653025871098066 0.0 48-49 0.0 1.0238275382988286E-4 0.0 0.43374453660029877 0.0 50-51 0.0 1.0238275382988286E-4 0.0 0.49676112158259167 0.0 52-53 0.0 1.0238275382988286E-4 0.0 0.5728826990551096 0.0 54-55 0.0 1.0238275382988286E-4 0.0 0.6689177221475398 0.0 56-57 0.0 1.0238275382988286E-4 0.0 0.774423149969234 0.0 58-59 0.0 1.0238275382988286E-4 0.0 0.8660045232700642 0.0 60-61 0.0 1.0238275382988286E-4 0.0 0.9462726022726924 1.0238275382988286E-4 62-63 0.0 1.0238275382988286E-4 0.0 1.0519316042251314 1.0238275382988286E-4 64-65 0.0 1.0238275382988286E-4 0.0 1.1940388665410089 1.0238275382988286E-4 66-67 0.0 1.0238275382988286E-4 0.0 1.3548821728077547 1.0238275382988286E-4 68-69 0.0 1.0238275382988286E-4 0.0 1.528728088810896 1.0238275382988286E-4 70-71 0.0 1.0238275382988286E-4 0.0 1.6943321931307316 1.0238275382988286E-4 72-73 0.0 1.0238275382988286E-4 0.0 1.8867093875770813 1.0238275382988286E-4 74-75 0.0 1.0238275382988286E-4 0.0 2.113435995933357 1.0238275382988286E-4 76-77 0.0 1.0238275382988286E-4 0.0 2.3637618290474207 1.0238275382988286E-4 78-79 0.0 1.0238275382988286E-4 0.0 2.616749613761061 1.0238275382988286E-4 80-81 0.0 1.0238275382988286E-4 0.0 2.885862682202908 1.0238275382988286E-4 82-83 0.0 1.0238275382988286E-4 0.0 3.1712546085037068 1.0238275382988286E-4 84-85 0.0 1.0238275382988286E-4 0.0 3.490842374583686 1.0238275382988286E-4 86-87 0.0 1.0238275382988286E-4 0.0 3.864181086424354 1.0238275382988286E-4 88-89 0.0 1.0238275382988286E-4 0.0 4.259480898961532 1.0238275382988286E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1310 0.0 54.029133 1 AATACCG 65 2.5900026E-6 43.843624 5 CGGAGAT 80 2.2613312E-7 41.564358 1 AGCGTAA 50 0.001616155 37.997807 8 GTATCAA 2355 0.0 37.315895 1 ATCAACG 2585 0.0 33.993977 3 TCAACGC 2595 0.0 33.67994 4 CAACGCA 2730 0.0 32.012806 5 AACGCAG 2750 0.0 31.952703 6 ATACCGT 90 2.4135465E-5 31.66484 6 TATCAAC 2830 0.0 30.883192 2 ACGCAGA 2985 0.0 29.278046 7 CGCAGAG 3000 0.0 29.131653 8 GTGGTAT 660 0.0 27.349707 1 CGTATAC 90 8.946921E-4 26.388716 3 GCAGAGT 3445 0.0 24.955019 9 TTTACGC 50 0.0016546139 23.74863 38-39 GAGTACT 2445 0.0 22.243092 12-13 CAGAGTA 3505 0.0 21.614304 10-11 TTAGGCG 165 6.324277E-5 20.150354 9 >>END_MODULE