FastQCFastQC Report
Fri 10 Feb 2017
SRR2031740_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031740_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910608
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.13079173475304412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.11541739145713634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.048.4661641
GTATCAA17750.039.8705861
TCAACGC19800.034.5431564
CAACGCA20250.034.0100825
ATCAACG20650.033.351293
TATCAAC21150.033.236562
AACGCAG21450.032.550286
ACGCAGA25250.027.0887957
CGCAGAG25450.026.502648
ACGTATA908.949582E-426.3871352
GTACATG22200.023.106571
ACATGGG22000.022.0212633
GTCTATA3059.094947E-1221.8018261
TACATGG23550.021.5803072
GCAGAGT29950.021.5690339
GTGGTAT4850.021.5449581
CATGGGG15900.020.3131144
GAGTACT15050.019.88567712-13
CAGAGTA28850.018.02937710-11
GTACTTT16350.018.01499414-15
Produced by FastQC (version 0.11.5)