##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031740_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 910608 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01126060829687 33.0 31.0 34.0 30.0 34.0 2 32.3188452111117 34.0 31.0 34.0 31.0 34.0 3 32.42869159945882 34.0 31.0 34.0 31.0 34.0 4 35.846663987138264 37.0 37.0 37.0 35.0 37.0 5 35.84626425421257 37.0 37.0 37.0 35.0 37.0 6 35.867004243318746 37.0 37.0 37.0 35.0 37.0 7 35.85183306098782 37.0 37.0 37.0 35.0 37.0 8 35.8360853407833 37.0 37.0 37.0 35.0 37.0 9 37.62581264385992 39.0 39.0 39.0 35.0 39.0 10-11 37.55596590409924 39.0 38.0 39.0 35.0 39.0 12-13 37.50596140161299 39.0 38.0 39.0 35.0 39.0 14-15 38.92757641048618 41.0 39.0 41.0 36.0 41.0 16-17 38.85811897106109 41.0 39.0 41.0 36.0 41.0 18-19 38.85614226978019 41.0 39.0 41.0 36.0 41.0 20-21 38.852095522991235 41.0 39.0 41.0 36.0 41.0 22-23 38.747619173123894 41.0 39.0 41.0 35.0 41.0 24-25 38.68007913394128 41.0 39.0 41.0 35.0 41.0 26-27 38.56691573102806 40.5 39.0 41.0 35.0 41.0 28-29 38.4531071547801 40.0 38.0 41.0 34.0 41.0 30-31 38.31547987718096 40.0 38.0 41.0 34.0 41.0 32-33 38.137408742290866 40.0 38.0 41.0 34.0 41.0 34-35 37.97908869678281 40.0 38.0 41.0 33.5 41.0 36-37 37.819773711630035 40.0 38.0 41.0 33.0 41.0 38-39 37.63946835520883 40.0 38.0 41.0 33.0 41.0 40-41 37.556612724685046 40.0 38.0 41.0 33.0 41.0 42-43 37.54537847240525 40.0 38.0 41.0 33.0 41.0 44-45 37.434706811273344 40.0 38.0 41.0 32.5 41.0 46-47 37.395352336021645 40.0 37.5 41.0 32.5 41.0 48-49 37.46753268146118 40.0 38.0 41.0 33.0 41.0 50-51 36.9191979424736 39.5 37.0 40.5 32.0 41.0 52-53 36.93453494807865 39.5 37.0 40.5 32.0 41.0 54-55 37.15086348900954 40.0 37.0 41.0 32.0 41.0 56-57 37.01310113682287 40.0 36.5 41.0 32.0 41.0 58-59 36.81156326322633 40.0 36.0 41.0 31.5 41.0 60-61 36.565949892818864 39.0 35.5 41.0 31.0 41.0 62-63 36.276936398538126 39.0 35.0 41.0 31.0 41.0 64-65 35.98982657740763 39.0 35.0 41.0 30.5 41.0 66-67 35.69122882733295 38.0 35.0 40.5 30.0 41.0 68-69 35.345470279198075 37.0 35.0 40.0 30.0 41.0 70-71 34.9735176936728 37.0 35.0 39.5 29.0 41.0 72-73 34.59721636532954 36.0 35.0 39.0 29.0 41.0 74-75 34.19407637534482 36.0 34.0 39.0 29.0 40.5 76-77 33.82289580148648 35.0 34.0 37.5 29.0 39.5 78-79 33.439889063131446 35.0 34.0 37.0 28.0 39.0 80-81 33.10240246077346 35.0 34.0 36.5 27.5 39.0 82-83 32.79388167026866 35.0 34.0 36.0 27.0 37.5 84-85 32.496509474988144 35.0 34.0 36.0 26.5 37.0 86-87 32.24363337462442 35.0 34.0 35.0 26.0 37.0 88-89 31.99287893363555 35.0 33.5 35.0 25.5 36.0 90-91 31.77943253298895 35.0 33.0 35.0 25.0 36.0 92-93 31.57886653752218 35.0 33.0 35.0 25.0 36.0 94-95 31.348112469910212 35.0 33.0 35.0 24.0 35.5 96-97 31.107086693725513 35.0 33.0 35.0 23.0 35.0 98-99 30.865655144694536 35.0 33.0 35.0 19.5 35.0 100-101 30.27441335898652 34.5 32.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07598651728726225 1101 2 0.004134169817604061 1101 3 0.1851928209401521 1101 4 0.12993512081818892 1101 5 0.033016423646252235 1101 6 0.18349274042764563 1101 7 0.26726037394822555 1101 8 0.22406373025182802 1101 9 0.26524428312400516 1101 10-11 0.09200884239855611 1101 12-13 0.047620751707619036 1101 14-15 0.022576009179445578 1101 16-17 0.06324707958602005 1101 18-19 0.27761801571187306 1101 20-21 0.16122505496007733 1101 22-23 0.12397492365316509 1101 24-25 0.1659456991576107 1101 26-27 0.021480846377308183 1101 28-29 -0.006102673395311342 1101 30-31 -0.11964194894405722 1101 32-33 -0.030248752889448838 1101 34-35 0.0835649378130725 1101 36-37 -0.27084459103520686 1101 38-39 -0.08621657470619226 1101 40-41 -0.4040875271897164 1101 42-43 -0.28843957831603007 1101 44-45 -0.5360566034262106 1101 46-47 -0.6249750770435298 1101 48-49 -0.42164751795932887 1101 50-51 -0.1921404855161981 1101 52-53 -0.2452286719593033 1101 54-55 -0.2319777089875359 1101 56-57 -0.5081306129437806 1101 58-59 -0.5848288169842988 1101 60-61 -0.7673912258691473 1101 62-63 -0.4386207454140987 1101 64-65 -0.18980033552966802 1101 66-67 -0.6550797713552683 1101 68-69 -0.5438541930418879 1101 70-71 -0.6637265061481443 1101 72-73 -0.27678021332747704 1101 74-75 -0.13394656764940294 1101 76-77 -0.22868921003152565 1101 78-79 -0.4440208365608953 1101 80-81 -0.2826812508516312 1101 82-83 -0.3049247560810002 1101 84-85 -0.4527205275749111 1101 86-87 -0.6116097209797999 1101 88-89 -0.6928254046169293 1101 90-91 -1.0031005658842282 1101 92-93 -0.9368719379129224 1101 94-95 -0.8267569985816792 1101 96-97 -0.7123296326973367 1101 98-99 -0.7969155539730757 1101 100-101 -0.9791064865613244 1102 1 0.12774903202576127 1102 2 0.1669218039103626 1102 3 0.2934212121118378 1102 4 0.29073784379580303 1102 5 0.253111913002499 1102 6 0.27709687616351886 1102 7 0.10924542747851973 1102 8 0.07718386810969236 1102 9 0.23143848753958451 1102 10-11 0.21258551693365035 1102 12-13 0.376855199083451 1102 14-15 0.3666207417772043 1102 16-17 0.24698754222163188 1102 18-19 0.26016147903462894 1102 20-21 0.34151195027426695 1102 22-23 0.3547631202857602 1102 24-25 0.20379964530332018 1102 26-27 0.26486477269171615 1102 28-29 0.2638781717673808 1102 30-31 0.19915613842051272 1102 32-33 0.3311546059717969 1102 34-35 0.46264379664049216 1102 36-37 0.27635979962378343 1102 38-39 -0.035667208275576456 1102 40-41 0.15685903508665433 1102 42-43 0.238415467986691 1102 44-45 -0.07557680679504131 1102 46-47 0.1527887559177401 1102 48-49 0.38534079331746085 1102 50-51 0.40855058398595645 1102 52-53 0.40496056216763776 1102 54-55 0.5064795433258169 1102 56-57 0.45658336403480604 1102 58-59 0.19065281324188987 1102 60-61 -0.12072409635325698 1102 62-63 0.20975180549041283 1102 64-65 0.09773050625066304 1102 66-67 0.11288144900012753 1102 68-69 0.09552128093351797 1102 70-71 0.01687993315274383 1102 72-73 0.21909419787489526 1102 74-75 0.06892915021195023 1102 76-77 0.3603914645890285 1102 78-79 0.4520534899044648 1102 80-81 0.2478027114226009 1102 82-83 0.32760588987850525 1102 84-85 0.4609643219456885 1102 86-87 0.3816914265302458 1102 88-89 0.15144541202712958 1102 90-91 0.05995997123450181 1102 92-93 0.381205172768567 1102 94-95 0.21016857882273854 1102 96-97 0.06517607280950344 1102 98-99 -0.0753603093572579 1102 100-101 -0.3052122511704489 1103 1 0.15664003615375321 1103 2 0.35287094658433205 1103 3 0.38535724466471777 1103 4 0.29594035068123503 1103 5 0.3223031284363316 1103 6 0.26493861266578733 1103 7 0.40729788104683706 1103 8 0.41906102287322966 1103 9 0.2801843906267436 1103 10-11 0.3126139745318284 1103 12-13 0.465687881490112 1103 14-15 0.6458756433531647 1103 16-17 0.5827814765009336 1103 18-19 0.511862392948764 1103 20-21 0.4102395031170829 1103 22-23 0.5302401939287904 1103 24-25 0.5452147729699917 1103 26-27 0.5185241908483107 1103 28-29 0.5410486360514568 1103 30-31 0.4288498430764349 1103 32-33 0.568101727174998 1103 34-35 0.4558311457661617 1103 36-37 0.31685136374496636 1103 38-39 0.42287955704409086 1103 40-41 0.6661838049726256 1103 42-43 0.5394355256869332 1103 44-45 0.5476740322134575 1103 46-47 0.6399452203719136 1103 48-49 0.4635926463323301 1103 50-51 0.44414466088034743 1103 52-53 0.4046841636565901 1103 54-55 0.022657851439824128 1103 56-57 0.16592906824588027 1103 58-59 0.17289216453949052 1103 60-61 0.1865255585574772 1103 62-63 0.26648464425031904 1103 64-65 0.4342685903673953 1103 66-67 0.2992018488457404 1103 68-69 0.36891538922940015 1103 70-71 0.5494647008144398 1103 72-73 0.41612381340293325 1103 74-75 0.3885758507660526 1103 76-77 0.4209644436089093 1103 78-79 0.361151907400334 1103 80-81 0.4411034002927323 1103 82-83 0.40971423809482843 1103 84-85 0.20004330977106832 1103 86-87 0.415322092699725 1103 88-89 0.4843510636407089 1103 90-91 0.5753622373751917 1103 92-93 -0.1648282632737974 1103 94-95 0.3695180446703503 1103 96-97 0.3708826389914073 1103 98-99 0.30149270551265417 1103 100-101 0.3741123224562237 1104 1 0.06540554006495114 1104 2 0.16751475804707638 1104 3 0.21703793831894558 1104 4 0.13215936558074048 1104 5 0.1781644703009846 1104 6 0.13805648177551433 1104 7 0.1806302449123507 1104 8 0.11490261183099193 1104 9 0.21753101778759998 1104 10-11 0.26539028800925024 1104 12-13 0.17417269417074976 1104 14-15 0.30686713627302 1104 16-17 0.31098738540731574 1104 18-19 0.2942759436852569 1104 20-21 0.2752039963580728 1104 22-23 0.23775023700951436 1104 24-25 0.228196522738223 1104 26-27 0.23426637905837566 1104 28-29 0.08593802583205701 1104 30-31 0.21697416520635926 1104 32-33 0.2898462845220493 1104 34-35 0.16191804037894286 1104 36-37 0.14999686268764378 1104 38-39 0.18416931279879378 1104 40-41 0.23785853034056004 1104 42-43 0.2754971535445847 1104 44-45 0.34659428742585874 1104 46-47 0.14237040472227847 1104 48-49 0.33206312906671087 1104 50-51 0.3120705753611688 1104 52-53 0.1145845508377974 1104 54-55 0.3717662440015843 1104 56-57 0.40924504204600964 1104 58-59 0.37463766105849317 1104 60-61 0.389787363343018 1104 62-63 0.4122039893748024 1104 64-65 0.4300763994307175 1104 66-67 0.4462049814386404 1104 68-69 0.44492318132702025 1104 70-71 0.4123190867474591 1104 72-73 0.48808710692748747 1104 74-75 0.4260223028167829 1104 76-77 0.33522031646320016 1104 78-79 -0.02232942125532844 1104 80-81 0.22583155545606104 1104 82-83 0.36768174880442217 1104 84-85 0.37082794249423756 1104 86-87 0.264516828489441 1104 88-89 0.24520077189148992 1104 90-91 0.277951473858689 1104 92-93 0.12933932741688636 1104 94-95 0.3115196321739049 1104 96-97 0.3574191546028693 1104 98-99 0.3044170143123921 1104 100-101 0.3192836153021581 1105 1 -0.08680071123944089 1105 2 0.24072047205367397 1105 3 0.16751329144792493 1105 4 0.20457467457137568 1105 5 0.2254200331093159 1105 6 0.3143390027234645 1105 7 0.09883220671535753 1105 8 0.15632338704957505 1105 9 0.22746880669617298 1105 10-11 0.19773517102487403 1105 12-13 0.10944829241409337 1105 14-15 0.2762433823820487 1105 16-17 0.307927896328664 1105 18-19 0.145366747012055 1105 20-21 0.19376119637050238 1105 22-23 0.08789077812611623 1105 24-25 0.22153136583394684 1105 26-27 0.22197735725118406 1105 28-29 0.024690518050114463 1105 30-31 -0.01500526650131917 1105 32-33 0.035957536248282906 1105 34-35 0.04779776560219062 1105 36-37 0.20866667321246268 1105 38-39 0.19019948140373089 1105 40-41 0.10380051106001531 1105 42-43 -0.024410484520963394 1105 44-45 0.04818087007868854 1105 46-47 0.28430361105795043 1105 48-49 0.23707465210613066 1105 50-51 0.2002837715725505 1105 52-53 0.2703054401766636 1105 54-55 0.1173357574584557 1105 56-57 0.21526943803004173 1105 58-59 0.21604822317855366 1105 60-61 0.10203109227850149 1105 62-63 0.15508479363410999 1105 64-65 0.3268597174443926 1105 66-67 0.33260424254316234 1105 68-69 0.2354525306638422 1105 70-71 0.25344989877760327 1105 72-73 -0.01333828638781398 1105 74-75 0.2374751826885344 1105 76-77 0.24949689605210779 1105 78-79 0.23944783550641802 1105 80-81 0.29332459500739105 1105 82-83 0.19277577591797979 1105 84-85 0.28561517700918415 1105 86-87 0.34854842692817556 1105 88-89 0.36142139258144823 1105 90-91 0.1745149882302961 1105 92-93 0.22808000796448624 1105 94-95 0.4051360015505949 1105 96-97 0.30112277936474996 1105 98-99 0.32997862371910003 1105 100-101 0.4138968719040399 1106 1 0.09644500835436531 1106 2 0.0910382038078481 1106 3 0.13357405104849107 1106 4 0.23439712480043795 1106 5 0.19839277784721787 1106 6 0.20567263162121918 1106 7 0.2107202300227229 1106 8 0.2599102724339133 1106 9 0.18158876362821275 1106 10-11 0.21566326442322747 1106 12-13 0.1977587195046837 1106 14-15 0.076403831639972 1106 16-17 0.15278190861570096 1106 18-19 0.13805574990436753 1106 20-21 0.16379940862798037 1106 22-23 0.23590521855933133 1106 24-25 0.2637632994586241 1106 26-27 0.22335595416465281 1106 28-29 0.32208743995777667 1106 30-31 0.26573660019912637 1106 32-33 0.12069585617566503 1106 34-35 0.09745892210354867 1106 36-37 0.09630574682911686 1106 38-39 -0.054981762922487576 1106 40-41 -0.06811242410581997 1106 42-43 -0.06117447632960449 1106 44-45 -0.1371760239614659 1106 46-47 -0.0439354583363496 1106 48-49 0.04529978774909438 1106 50-51 0.07317629168148443 1106 52-53 0.09112584256650536 1106 54-55 0.006260536312929332 1106 56-57 0.09585085547256966 1106 58-59 0.16741943098146095 1106 60-61 0.01030267375927707 1106 62-63 0.006487669053704792 1106 64-65 0.23128051673504046 1106 66-67 0.27590654161213024 1106 68-69 0.24102814237664205 1106 70-71 0.13078221874719986 1106 72-73 0.05093641963114948 1106 74-75 0.25417879490802164 1106 76-77 0.33788965894610357 1106 78-79 0.2387849267302613 1106 80-81 0.42158477614616174 1106 82-83 0.32794371984518733 1106 84-85 0.12104912891663133 1106 86-87 0.16034989208323935 1106 88-89 0.1633510060254011 1106 90-91 0.15760448091507584 1106 92-93 0.1443001612357513 1106 94-95 0.34195488389484296 1106 96-97 0.37945579602191515 1106 98-99 0.41053470706826545 1106 100-101 0.18375352315912963 1107 1 -0.16058211023976554 1107 2 0.15451132826466818 1107 3 0.19289468681085964 1107 4 0.2880951818658559 1107 5 0.26225920293596516 1107 6 0.3213531006359105 1107 7 0.23482204627620717 1107 8 0.288146900948945 1107 9 0.2737227509895632 1107 10-11 0.1993538090046627 1107 12-13 0.2711453767439167 1107 14-15 0.3562666919814177 1107 16-17 0.3610368027780595 1107 18-19 0.41823531300186545 1107 20-21 0.3801508455746756 1107 22-23 0.3013964024384208 1107 24-25 0.3351959743448347 1107 26-27 0.15180985550069437 1107 28-29 0.2584147191128423 1107 30-31 0.2381554727373114 1107 32-33 0.25637647249395457 1107 34-35 0.3577510251571496 1107 36-37 0.39650976232545787 1107 38-39 0.3375970813334064 1107 40-41 0.3029748238208825 1107 42-43 0.4764306616202205 1107 44-45 0.44326721259339763 1107 46-47 0.46237576472549335 1107 48-49 0.2863923386056584 1107 50-51 0.31287535041143855 1107 52-53 0.3830440301429192 1107 54-55 0.37611853563533515 1107 56-57 0.46418352593966716 1107 58-59 0.47976420980509005 1107 60-61 0.49504791217016475 1107 62-63 0.6437429609358105 1107 64-65 0.4624153437269598 1107 66-67 0.31085947810954906 1107 68-69 0.3231999867651041 1107 70-71 0.4430275676357951 1107 72-73 0.48462401814042977 1107 74-75 0.38291920176057914 1107 76-77 0.378495371380005 1107 78-79 0.459219619675892 1107 80-81 0.5907106765394587 1107 82-83 0.6094666918427691 1107 84-85 0.6474459353744209 1107 86-87 0.7394835995677411 1107 88-89 0.6282117304645425 1107 90-91 0.6280185513556624 1107 92-93 0.9147076030140049 1107 94-95 0.7898565440065042 1107 96-97 0.8822486724194576 1107 98-99 0.8030111753169749 1107 100-101 0.7574414162756362 1108 1 -0.1311959714047113 1108 2 0.0806843442883931 1108 3 -0.03362684105612601 1108 4 -0.03205818568567764 1108 5 -0.08304677817513806 1108 6 0.008879280912594822 1108 7 -0.03424417655914169 1108 8 -0.05019722098551682 1108 9 0.06250446752209626 1108 10-11 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48-49 -0.2872750204836123 2316 50-51 -0.42478167140804857 2316 52-53 -0.316025083992308 2316 54-55 -0.23338782701964078 2316 56-57 -0.22557059588309158 2316 58-59 -0.24105487979548457 2316 60-61 -0.3745329949579741 2316 62-63 -0.2052462111012261 2316 64-65 -0.28404757622462284 2316 66-67 -0.10040070377405641 2316 68-69 -0.20525612890763512 2316 70-71 -0.10170524992447838 2316 72-73 -0.002637382297919544 2316 74-75 -0.01242616446010203 2316 76-77 -0.033329107667398716 2316 78-79 -0.07597023769595523 2316 80-81 -0.1556694389370037 2316 82-83 0.11757641901736093 2316 84-85 0.08609906436129933 2316 86-87 -0.024179138827371105 2316 88-89 0.07910867671728283 2316 90-91 0.045021888069324945 2316 92-93 0.023049591629916932 2316 94-95 0.13726323051898248 2316 96-97 0.03464775318755997 2316 98-99 0.11483238307646815 2316 100-101 0.025240289316950992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6112.0 3 776.0 4 646.0 5 611.0 6 718.0 7 788.0 8 929.0 9 1061.0 10 1234.0 11 1318.0 12 1719.0 13 1998.0 14 2156.0 15 2384.0 16 2758.0 17 3420.0 18 3424.0 19 2372.0 20 2281.0 21 2476.0 22 2690.0 23 3052.0 24 3556.0 25 4002.0 26 4767.0 27 5739.0 28 7056.0 29 8744.0 30 10941.0 31 13926.0 32 18330.0 33 24779.0 34 35974.0 35 57193.0 36 102824.0 37 202516.0 38 280357.0 39 84868.0 40 113.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.37897855558403 19.63661398401594 13.886686293895947 24.097721166504087 2 16.29705557584103 21.30550215343736 38.56049641240415 23.83694585831746 3 18.298861648064385 25.371358313070424 30.38028278989153 25.949497248973657 4 13.710045498887549 18.127847804736415 36.89776112919066 31.26434556718537 5 14.649563156708254 36.55847761899787 34.49319030312361 14.298768921170268 6 31.160516576766362 36.04327968634212 18.40530122283475 14.390902514056773 7 26.67327856162697 32.247281939410996 23.119568276978292 17.959871221983743 8 25.11843443366407 33.835362669378554 21.5708435258225 19.47535937113487 9 25.749478616401294 16.183505039033687 21.378101778915763 36.68891456564926 10-11 24.2911744305047 25.897017617721712 29.139208252309256 20.672599699464335 12-13 25.074954895129114 24.244871348044686 28.401665884225885 22.27850787260032 14-15 22.874569278113693 26.10937518041247 26.01876344674279 24.99729209473105 16-17 22.43309229880949 28.200709356355336 27.002007093563552 22.364191251271617 18-19 22.326943559903505 27.58768777669207 28.2976911144556 21.787677548948825 20-21 23.237391045691943 26.931487100876584 27.97554607049721 21.855575782934263 22-23 23.103025318286 26.808486307255606 27.594078343686757 22.494410030771633 24-25 22.902635681413905 26.890568759248634 27.80234917996381 22.404446379373656 26-27 22.715466273947797 27.110410403311946 27.77067724764762 22.403446075092642 28-29 22.699492743544397 26.980908751561984 27.860072644270122 22.459525860623497 30-31 23.02183108462809 26.80405344121988 27.676307377713773 22.49780809643826 32-33 22.861746090902795 26.970049768636855 27.55134348934542 22.61686065111493 34-35 23.085135860242957 27.047964515362892 27.36117097543168 22.505728648962467 36-37 22.97003977177139 26.89262281472424 27.4823270169852 22.655010396519174 38-39 22.940148819863758 26.9791524655986 27.519251151111092 22.56144756342655 40-41 23.08211594766973 26.921677216059702 27.44216524780354 22.554041588467022 42-43 23.04055866560539 26.945589030734524 27.262268083852607 22.751584219807476 44-45 23.092972815464588 26.999145071239482 27.122408754910342 22.785473358385584 46-47 23.069864715011974 26.865563326426233 27.129953746795454 22.934618211766335 48-49 23.002201630658313 26.965081829838805 27.196187066024724 22.83652947347816 50-51 23.026989998328656 27.007316526068557 27.104683280407453 22.861010195195327 52-53 23.121351378093426 26.99881265185413 26.955991160852694 22.92384480919975 54-55 23.072847187067318 27.056678588644495 26.996711424926445 22.873762799361742 56-57 23.08954638838601 27.160621425535975 26.84099156724734 22.908840618830673 58-59 23.07557841351791 26.998274475485072 27.06918644185191 22.856960669145103 60-61 23.112708411092683 26.96009907755193 27.202735736575967 22.72445677477942 62-63 23.05998372875393 27.08706216055762 27.001803029969878 22.851151080718573 64-65 22.950878259832074 27.05371276254737 27.03364422160728 22.961764756013277 66-67 23.04789487833721 27.1626970055324 26.867467031278437 22.921941084851955 68-69 22.960707136294836 27.066535666629466 27.038715569193144 22.934041627882557 70-71 23.011631787064466 26.89823959205461 27.022094293393263 23.068034327487663 72-73 23.012167943346004 26.905975782857126 27.05099717323734 23.030859100559525 74-75 22.952074829969362 26.98784969368652 26.91386836600201 23.146207110342107 76-77 22.909654498252205 26.961528692116275 27.023380553334995 23.105436256296517 78-79 22.932335787612487 27.01284977876301 26.96100423121245 23.093810202412055 80-81 22.915072264886216 26.961334107442887 26.94555502775359 23.17803859991731 82-83 22.94037921753737 27.01472718825344 26.960953844546655 23.08393974966254 84-85 23.042033549431313 27.02927218669458 26.855268597767477 23.07342566610663 86-87 22.855165049899572 27.088780059744234 26.913220211870442 23.142834678485755 88-89 23.058024523040928 27.0476915092929 26.810586336408974 23.083697631257202 90-91 22.941257647040068 27.006978619901357 26.962169865315328 23.08959386774325 92-93 22.90522115869461 26.93836568181456 26.98944946636966 23.166963693121176 94-95 22.939761685518338 26.940916150135376 26.91408859141573 23.205233572930553 96-97 22.842704324320167 26.88648288553309 27.012462774921424 23.258350015225318 98-99 22.799977792961137 26.91113831412296 26.964512162476375 23.324371730439527 100-101 22.943268476199222 26.971519577300352 26.947872505990134 23.137339440510292 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1197.0 1 675.5 2 138.5 3 105.5 4 81.5 5 79.0 6 116.5 7 192.0 8 293.5 9 324.0 10 258.0 11 224.5 12 234.0 13 263.5 14 292.5 15 312.0 16 331.5 17 370.5 18 406.5 19 434.0 20 502.5 21 593.5 22 676.0 23 787.0 24 945.0 25 1197.5 26 1585.5 27 2166.0 28 2958.0 29 4128.5 30 6089.0 31 8897.0 32 12566.5 33 16805.0 34 21007.0 35 24986.0 36 28515.0 37 30633.5 38 31412.5 39 31467.5 40 31256.5 41 31267.5 42 31242.5 43 31504.5 44 32071.0 45 32719.0 46 33498.0 47 33938.5 48 34193.0 49 34487.5 50 34044.0 51 32781.5 52 31741.0 53 30977.5 54 29698.5 55 28146.0 56 26364.5 57 23659.0 58 21101.0 59 18688.0 60 15900.5 61 13230.0 62 10480.5 63 8028.5 64 6086.5 65 4679.0 66 3640.5 67 2768.0 68 2106.5 69 1568.0 70 1153.5 71 884.5 72 678.5 73 542.0 74 441.0 75 342.5 76 256.0 77 200.0 78 157.5 79 110.5 80 74.5 81 50.5 82 38.5 83 32.5 84 27.5 85 20.5 86 16.5 87 15.0 88 10.5 89 8.0 90 6.5 91 4.0 92 4.0 93 4.5 94 3.5 95 3.5 96 3.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14572681109764027 2 0.14979003039727304 3 0.14396974329239365 4 0.1488016797568218 5 0.1365022051200956 6 0.11882171032980163 7 0.12475181417250891 8 0.1134406901762339 9 0.11080508846836398 10-11 0.0987252473072936 12-13 0.11651555883541546 14-15 0.1354589461107304 16-17 0.14638571152460772 18-19 0.14457373535044718 20-21 0.1344705954702792 22-23 0.12810122467626026 24-25 0.11135417215750357 26-27 0.11547229982605028 28-29 0.1216220371444134 30-31 0.11096981357510587 32-33 0.10970692109008487 34-35 0.12563034807513224 36-37 0.12837576652083002 38-39 0.11552720819496426 40-41 0.11827262664066206 42-43 0.11113453868184774 44-45 0.12908957531671147 46-47 0.12711287403580904 48-49 0.1407850578953842 50-51 0.12738741588037883 52-53 0.1117934391088152 54-55 0.1036120921406357 56-57 0.12283002126052044 58-59 0.11305633159383621 60-61 0.11173853073990125 62-63 0.11289160648709434 64-65 0.1344705954702792 66-67 0.12601470665752992 68-69 0.1310662765976139 70-71 0.11799808479609228 72-73 0.1193707940189412 74-75 0.10092158206385185 76-77 0.12925430042345334 78-79 0.12914448368562542 80-81 0.12914448368562542 82-83 0.13567857958638624 84-85 0.12563034807513224 86-87 0.1352393126350746 88-89 0.12035914465939242 90-91 0.1304073761706464 92-93 0.14468355208827507 94-95 0.12030423629047844 96-97 0.10311791682041009 98-99 0.10833421186723596 100-101 0.1542376082793035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 910608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.387104255294545 #Duplication Level Percentage of deduplicated Percentage of total 1 68.09780286175133 42.484247266925756 2 19.369073320864114 24.167607931943873 3 6.8415649081565615 12.804762695835842 4 2.7189177695492086 6.785016254017576 5 1.2367154607457302 3.857754819183925 6 0.6251388813107419 2.3400362737422875 7 0.3451459873997235 1.5072861089442189 8 0.22428607150507324 1.119404682079796 9 0.1418662056908822 0.7965559588266116 >10 0.3934756224919041 3.5530549080575615 >50 0.004598687765247772 0.17797745555918237 >100 0.00106066720388344 0.15903322305526477 >500 0.0 0.0 >1k 3.535555654858969E-4 0.24726242182813268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1191 0.13079173475304412 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1051 0.11541739145713634 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.6472510674186915E-4 0.0 22-23 0.0 0.0 0.0 3.2945021348373835E-4 0.0 24-25 0.0 0.0 0.0 4.392669513116511E-4 0.0 26-27 0.0 0.0 0.0 0.0010432590093651713 0.0 28-29 0.0 0.0 0.0 0.0018119761741605608 0.0 30-31 0.0 0.0 0.0 0.0037337690861490346 0.0 32-33 0.0 0.0 0.0 0.007357721434470156 0.0 34-35 0.0 0.0 0.0 0.014825259606768226 0.0 36-37 0.0 0.0 0.0 0.023336056788431467 0.0 38-39 0.0 0.0 0.0 0.032670479503804056 0.0 40-41 0.0 0.0 0.0 0.04019292604501608 0.0 42-43 0.0 0.0 0.0 0.053206209477623743 0.0 44-45 0.0 5.490836891395639E-5 0.0 0.07039252894769209 0.0 46-47 0.0 1.0981673782791277E-4 0.0 0.08829265721364188 0.0 48-49 0.0 1.0981673782791277E-4 0.0 0.1151977579814805 0.0 50-51 0.0 1.0981673782791277E-4 0.0 0.1395221654103632 0.0 52-53 0.0 1.0981673782791277E-4 0.0 0.16576836575123433 0.0 54-55 0.0 1.0981673782791277E-4 0.0 0.20178825575878973 0.0 56-57 0.0 1.0981673782791277E-4 0.0 0.2384670461933126 0.0 58-59 0.0 1.0981673782791277E-4 0.0 0.2760792788993727 0.0 60-61 0.0 1.0981673782791277E-4 0.0 0.318468539700947 0.0 62-63 0.0 1.0981673782791277E-4 0.0 0.3522920949519442 0.0 64-65 0.0 2.1963347565582555E-4 0.0 0.39638351518985115 0.0 66-67 0.0 2.1963347565582555E-4 0.0 0.4673800361955968 0.0 68-69 0.0 2.1963347565582555E-4 0.0 0.5544647092931316 0.0 70-71 0.0 2.1963347565582555E-4 0.0 0.6411101154393548 0.0 72-73 0.0 2.1963347565582555E-4 0.0 0.7329169082634899 5.490836891395639E-5 74-75 0.0 2.1963347565582555E-4 0.0 0.8330697731625463 1.0981673782791277E-4 76-77 0.0 2.1963347565582555E-4 0.0 0.9483773478818547 1.0981673782791277E-4 78-79 0.0 2.1963347565582555E-4 0.0 1.0720859030449985 1.0981673782791277E-4 80-81 5.490836891395639E-5 2.1963347565582555E-4 0.0 1.2094117336988035 1.0981673782791277E-4 82-83 1.0981673782791277E-4 2.1963347565582555E-4 0.0 1.354754186214046 1.0981673782791277E-4 84-85 1.0981673782791277E-4 2.1963347565582555E-4 0.0 1.514647356491487 1.0981673782791277E-4 86-87 1.0981673782791277E-4 2.1963347565582555E-4 0.0 1.717533779628556 1.0981673782791277E-4 88-89 1.0981673782791277E-4 2.1963347565582555E-4 0.0 1.9514983395709242 1.0981673782791277E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 980 0.0 48.466164 1 GTATCAA 1775 0.0 39.870586 1 TCAACGC 1980 0.0 34.543156 4 CAACGCA 2025 0.0 34.010082 5 ATCAACG 2065 0.0 33.35129 3 TATCAAC 2115 0.0 33.23656 2 AACGCAG 2145 0.0 32.55028 6 ACGCAGA 2525 0.0 27.088795 7 CGCAGAG 2545 0.0 26.50264 8 ACGTATA 90 8.949582E-4 26.387135 2 GTACATG 2220 0.0 23.10657 1 ACATGGG 2200 0.0 22.021263 3 GTCTATA 305 9.094947E-12 21.801826 1 TACATGG 2355 0.0 21.580307 2 GCAGAGT 2995 0.0 21.569033 9 GTGGTAT 485 0.0 21.544958 1 CATGGGG 1590 0.0 20.313114 4 GAGTACT 1505 0.0 19.885677 12-13 CAGAGTA 2885 0.0 18.029377 10-11 GTACTTT 1635 0.0 18.014994 14-15 >>END_MODULE