FastQCFastQC Report
Fri 10 Feb 2017
SRR2031740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910608
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT24560.2697099081053538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.1667018080227716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.16011280375309683No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10640.11684500904889919RNA PCR Primer, Index 27 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.053.3179441
GTATCAA19800.037.668171
TCAACGC23350.031.124114
ATCAACG24000.030.2811623
CAACGCA24550.029.7929765
TATCAAC24850.029.2453882
AACGCAG25200.029.0245086
ACGCAGA28850.024.858557
CGCAGAG29250.024.5186048
GTGGTAT4950.022.0730691
GCAGAGT33550.020.2436189
GTACATG22100.020.2057821
GAGTACT17700.020.12595612-13
CAGAGTA30200.019.97401210-11
TACATGG21800.019.1742552
AGAGTAC28300.018.37791310-11
GTACTTT19600.017.81147214-15
ACATGGG22300.017.6793213
ATAGGGG1952.2747097E-417.0512333
CATGGGG16250.016.9537964
Produced by FastQC (version 0.11.5)