##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031740_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 910608 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76023601813294 34.0 31.0 34.0 31.0 34.0 2 32.92846098430938 34.0 31.0 34.0 31.0 34.0 3 32.986201526891925 34.0 31.0 34.0 31.0 34.0 4 36.383418551121885 37.0 37.0 37.0 35.0 37.0 5 36.34708897791366 37.0 37.0 37.0 35.0 37.0 6 36.370286665612426 37.0 37.0 37.0 35.0 37.0 7 36.31521247342435 37.0 37.0 37.0 35.0 37.0 8 36.306729130427144 37.0 37.0 37.0 35.0 37.0 9 38.160776096849574 39.0 39.0 39.0 37.0 39.0 10-11 38.12519163020751 39.0 39.0 39.0 37.0 39.0 12-13 38.07422568218158 39.0 39.0 39.0 35.0 39.0 14-15 39.58520515962961 41.0 40.0 41.0 37.0 41.0 16-17 39.55705308980373 41.0 40.0 41.0 37.0 41.0 18-19 39.55270709240419 41.0 39.5 41.0 37.0 41.0 20-21 39.531829832375735 41.0 39.5 41.0 37.0 41.0 22-23 39.445502345685526 41.0 39.0 41.0 36.0 41.0 24-25 39.37737698329029 41.0 39.0 41.0 36.0 41.0 26-27 39.2501010313988 41.0 39.0 41.0 36.0 41.0 28-29 39.14580917365101 40.5 39.0 41.0 36.0 41.0 30-31 38.99304585507704 40.0 38.5 41.0 35.5 41.0 32-33 38.815676998225356 40.0 38.0 41.0 35.0 41.0 34-35 38.689512940804384 40.0 38.0 41.0 35.0 41.0 36-37 38.59696708133467 40.0 38.0 41.0 35.0 41.0 38-39 38.68859652012721 40.0 38.0 41.0 35.0 41.0 40-41 38.624495941173365 40.0 38.0 41.0 35.0 41.0 42-43 38.53758258218684 40.0 38.0 41.0 35.0 41.0 44-45 38.377695451829986 40.0 38.0 41.0 34.0 41.0 46-47 38.19735056138316 40.0 38.0 41.0 34.0 41.0 48-49 37.983800383919316 40.0 38.0 41.0 33.0 41.0 50-51 37.84012604765168 40.0 38.0 41.0 33.0 41.0 52-53 37.91364231370689 40.0 38.0 41.0 33.5 41.0 54-55 37.82697219879465 40.0 37.0 41.0 33.5 41.0 56-57 37.63317805246605 40.0 37.0 41.0 33.0 41.0 58-59 37.436490235095675 40.0 36.5 41.0 33.0 41.0 60-61 37.15412779154148 39.0 36.0 41.0 33.0 41.0 62-63 36.87113829441428 39.0 35.5 41.0 32.0 41.0 64-65 36.538143196633456 39.0 35.0 41.0 32.0 41.0 66-67 36.167876847117526 38.0 35.0 40.5 31.0 41.0 68-69 35.76866006009172 37.0 35.0 40.0 31.0 41.0 70-71 35.41779942631736 37.0 35.0 39.5 31.0 41.0 72-73 35.02491247605995 36.0 35.0 39.0 30.5 41.0 74-75 34.64037599054697 36.0 35.0 39.0 30.0 40.5 76-77 33.715074982868586 35.0 33.5 37.0 29.0 39.0 78-79 33.92504678193031 35.0 34.0 37.0 30.0 39.0 80-81 33.7079709380985 35.0 34.0 37.0 30.0 39.0 82-83 33.444347073603566 35.0 34.0 36.0 30.0 37.5 84-85 33.16144433169926 35.0 34.0 36.0 29.0 37.0 86-87 32.92111204821394 35.0 34.0 35.5 29.0 37.0 88-89 32.70776503171507 35.0 34.0 35.0 29.0 36.0 90-91 32.5169474680653 35.0 34.0 35.0 29.0 36.0 92-93 32.3564102226205 35.0 34.0 35.0 29.0 36.0 94-95 32.186027357545726 35.0 34.0 35.0 28.5 36.0 96-97 32.023266872243596 35.0 34.0 35.0 27.0 35.0 98-99 31.81868432959078 35.0 33.0 35.0 27.0 35.0 100-101 31.08617758684308 34.5 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18267006260985852 1101 2 0.19054030846933756 1101 3 0.2049190673656156 1101 4 0.039214373131997604 1101 5 0.051292508617621024 1101 6 0.05635289510669139 1101 7 0.06326257189272155 1101 8 0.07230920890988557 1101 9 0.0905029438878131 1101 10-11 0.1551772423819031 1101 12-13 0.17891314728276342 1101 14-15 0.22680458685157845 1101 16-17 0.1895329666927097 1101 18-19 0.104558429152803 1101 20-21 0.1509672516371907 1101 22-23 0.07966925893455112 1101 24-25 0.04536059918214619 1101 26-27 0.1273180349389591 1101 28-29 0.20561299163760083 1101 30-31 0.3265255715765605 1101 32-33 0.37040815595431553 1101 34-35 0.033530297083082417 1101 36-37 0.23561446151057908 1101 38-39 0.24858753881114382 1101 40-41 -0.13384987296575446 1101 42-43 0.10731645433325099 1101 44-45 -0.04160944523500376 1101 46-47 0.29398523075143856 1101 48-49 0.23627419547844397 1101 50-51 0.2950561042917741 1101 52-53 0.08968063244541469 1101 54-55 0.019231766337775014 1101 56-57 -0.21096300975263205 1101 58-59 0.14871425499431012 1101 60-61 0.09963251311524601 1101 62-63 0.17949937887279788 1101 64-65 0.15950991373333778 1101 66-67 -0.2502751363903215 1101 68-69 -0.28485135863096644 1101 70-71 -0.11615052851971086 1101 72-73 0.08732191213092477 1101 74-75 -0.0932165475975637 1101 76-77 -0.09378272285269418 1101 78-79 -0.20595524609129967 1101 80-81 0.03062510810742225 1101 82-83 0.04273817643076683 1101 84-85 -0.1180264539194269 1101 86-87 0.10557031580614051 1101 88-89 0.05367568511315568 1101 90-91 -0.10049710357564123 1101 92-93 -0.26791517792523223 1101 94-95 -0.0908158122322078 1101 96-97 -0.02285088150365766 1101 98-99 -0.37966173022214633 1101 100-101 -0.6886016312776171 1102 1 0.10847533125823361 1102 2 0.06695422385337224 1102 3 0.1005803899038824 1102 4 0.04041422074778467 1102 5 0.15892097598140964 1102 6 0.01095984710210729 1102 7 0.08206315152236954 1102 8 -0.005835762589285309 1102 9 -0.04615685124799995 1102 10-11 -0.02103301934153734 1102 12-13 0.004317517291553941 1102 14-15 0.011745253140844625 1102 16-17 -0.024264212775293004 1102 18-19 -0.07507246638924414 1102 20-21 0.05139503113581867 1102 22-23 -0.012412814914817716 1102 24-25 -0.10897380582517258 1102 26-27 0.012565249832732661 1102 28-29 0.06979255577604704 1102 30-31 -0.08318856561906784 1102 32-33 -0.006218492055964475 1102 34-35 -0.08909181500475682 1102 36-37 -0.1051048773983041 1102 38-39 -0.4297135552932261 1102 40-41 -0.31531792390765645 1102 42-43 -0.2821231064584353 1102 44-45 -0.16917514312262227 1102 46-47 -0.35811681682233854 1102 48-49 -0.28183656078159913 1102 50-51 -0.3245396859164771 1102 52-53 -0.17092929602657847 1102 54-55 -0.07386145505725494 1102 56-57 0.037136638125915056 1102 58-59 -0.2734647679665656 1102 60-61 -0.14203442057981874 1102 62-63 -0.1970596219425289 1102 64-65 -0.0017280655381384236 1102 66-67 -0.04815777918820885 1102 68-69 -0.035573225408718656 1102 70-71 -0.1290951753123437 1102 72-73 -0.02998900505823343 1102 74-75 0.034638431057963714 1102 76-77 -0.0384882617338107 1102 78-79 -0.04805334164473862 1102 80-81 -0.20529982486845455 1102 82-83 -0.0553173227521242 1102 84-85 0.07734576586987174 1102 86-87 0.24275882717621045 1102 88-89 -0.14990587782187959 1102 90-91 -0.29366544896085145 1102 92-93 -0.11284466462544174 1102 94-95 -0.026677370467545813 1102 96-97 -0.25462174265244997 1102 98-99 -0.3021318141402709 1102 100-101 -0.546276383564269 1103 1 0.32582105509523274 1103 2 0.21271109783528175 1103 3 0.2975609406074682 1103 4 0.18183049326036382 1103 5 0.21894197572627405 1103 6 0.11917068192373392 1103 7 0.22975645057574923 1103 8 0.18954200384906983 1103 9 0.14843424250506843 1103 10-11 0.2030664775804638 1103 12-13 0.1695792944849117 1103 14-15 0.32286039847085846 1103 16-17 0.3727151930823851 1103 18-19 0.2789433259422509 1103 20-21 0.34378213297476634 1103 22-23 0.2395399650981389 1103 24-25 0.053609849700507084 1103 26-27 0.2573349395467801 1103 28-29 0.37230827287480395 1103 30-31 0.3453550595518422 1103 32-33 0.08890021210931565 1103 34-35 0.17786989480624982 1103 36-37 0.19170705385860742 1103 38-39 0.03892747990915524 1103 40-41 0.014091858964143 1103 42-43 0.10390911931825997 1103 44-45 0.13005486623240614 1103 46-47 0.05099304913256475 1103 48-49 0.20125161197891828 1103 50-51 0.24523703642240946 1103 52-53 0.09305990317006518 1103 54-55 0.23412498996724906 1103 56-57 0.3321503435668802 1103 58-59 0.22692447964070794 1103 60-61 0.037904281099507386 1103 62-63 0.05797770003393765 1103 64-65 0.3709446262889742 1103 66-67 0.4634525937698726 1103 68-69 0.24710231217820677 1103 70-71 0.23029157980185033 1103 72-73 0.2697431010002944 1103 74-75 0.4595872400911247 1103 76-77 0.3937303187881085 1103 78-79 0.46697030760063996 1103 80-81 0.25898588941726075 1103 82-83 0.4282655869882248 1103 84-85 0.3762748443016264 1103 86-87 0.3729866842209333 1103 88-89 0.28835258258934715 1103 90-91 0.5505267477694673 1103 92-93 0.5105321074324678 1103 94-95 0.3979337090240165 1103 96-97 0.27490628644626725 1103 98-99 0.3371893903684544 1103 100-101 0.32780937520688624 1104 1 0.16576646193846045 1104 2 0.01815556853614453 1104 3 0.13481001506865908 1104 4 0.09747012995229909 1104 5 0.12842549174369822 1104 6 0.05211380445840774 1104 7 0.1687814689047471 1104 8 0.13723770001784885 1104 9 -0.019253394962440495 1104 10-11 0.21799855424113446 1104 12-13 0.08068363951851154 1104 14-15 0.2860281951734578 1104 16-17 0.19009850854115484 1104 18-19 0.21208640121536604 1104 20-21 0.2516493643564388 1104 22-23 0.21529872514879145 1104 24-25 0.20432649849261963 1104 26-27 0.2634289536888943 1104 28-29 0.3125754218517187 1104 30-31 0.20097857884122305 1104 32-33 0.253786996445136 1104 34-35 0.16752991455915378 1104 36-37 0.10459506820672715 1104 38-39 0.02569727371545838 1104 40-41 -0.01010090759407234 1104 42-43 -0.040217420469311094 1104 44-45 -0.050187379539814 1104 46-47 0.052516173969713975 1104 48-49 -0.0468405056417609 1104 50-51 0.11165648879427437 1104 52-53 0.15020927392486527 1104 54-55 0.14422805651515347 1104 56-57 0.055400605626147126 1104 58-59 -0.04823775474026348 1104 60-61 0.09605871732367177 1104 62-63 0.11953133281163986 1104 64-65 0.09737368245144751 1104 66-67 0.13551343999409227 1104 68-69 0.06013741297257269 1104 70-71 0.18703513738520172 1104 72-73 0.09163519923162511 1104 74-75 -0.024527120756914655 1104 76-77 0.038120432639985324 1104 78-79 0.09685778874146678 1104 80-81 0.030862536755158487 1104 82-83 0.08095676907246485 1104 84-85 0.1425119155393375 1104 86-87 0.06758008335689425 1104 88-89 0.1850421529333275 1104 90-91 0.25892370242866747 1104 92-93 0.07279163396862032 1104 94-95 0.1444413526718833 1104 96-97 0.20319520603496244 1104 98-99 0.252733595071593 1104 100-101 0.12249502526535849 1105 1 0.0835066140692291 1105 2 0.0029646946057440005 1105 3 -0.08873694964286472 1105 4 -0.028896982094273937 1105 5 -0.023185735559906107 1105 6 -0.08278790556951776 1105 7 0.010886965771987889 1105 8 0.050017838649566215 1105 9 -0.12264991629227495 1105 10-11 -0.033809830268701546 1105 12-13 0.01594369609019708 1105 14-15 -0.02725638171563105 1105 16-17 0.044339830737776253 1105 18-19 -0.04444887171859335 1105 20-21 -0.06557391617961628 1105 22-23 0.01997987665344425 1105 24-25 0.10128743561101317 1105 26-27 -0.11855647266356328 1105 28-29 0.0784534596934634 1105 30-31 0.1513917652835559 1105 32-33 -0.06151355728242436 1105 34-35 -0.037799990725460475 1105 36-37 0.13440399445483564 1105 38-39 -2.197264667103127E-5 1105 40-41 -0.021053345638300414 1105 42-43 -0.07999299873050347 1105 44-45 -0.08258288400026714 1105 46-47 -0.060285278954559374 1105 48-49 -0.15106961652492146 1105 50-51 -0.1373366329058996 1105 52-53 -0.1538879547730616 1105 54-55 -0.08433220742684 1105 56-57 -0.25624099454437044 1105 58-59 -0.07383488796791227 1105 60-61 0.0665584042700047 1105 62-63 -0.04091644495133551 1105 64-65 -0.02839396511672021 1105 66-67 0.12569554398120175 1105 68-69 0.17003035305720715 1105 70-71 0.1380144847238043 1105 72-73 -0.048226524295358786 1105 74-75 -0.02731352046206581 1105 76-77 -0.24538630134431827 1105 78-79 0.09738176510035856 1105 80-81 0.11118633638304942 1105 82-83 0.03190059259011946 1105 84-85 0.12364432614141663 1105 86-87 0.2831963550430814 1105 88-89 0.20311045636004366 1105 90-91 0.10601141518304757 1105 92-93 0.18167880587283491 1105 94-95 -0.09207402692234723 1105 96-97 0.00989899509451675 1105 98-99 0.03864706792472816 1105 100-101 -0.06507963026458796 1106 1 0.07161225723852027 1106 2 0.022691508632384227 1106 3 -0.02478947566309131 1106 4 0.06820015304199956 1106 5 0.05828026397563235 1106 6 0.06157842542040726 1106 7 0.16469869123486802 1106 8 0.060295035854366574 1106 9 0.16107503990085803 1106 10-11 0.07149977346384873 1106 12-13 3.3578340836726284E-4 1106 14-15 0.09465338668852752 1106 16-17 0.17786528094572418 1106 18-19 0.17806469257723023 1106 20-21 0.19598159715244634 1106 22-23 0.034335862888589475 1106 24-25 0.1979412241135563 1106 26-27 0.029897092054490315 1106 28-29 0.1024828472417596 1106 30-31 0.15031717749036488 1106 32-33 0.18045236836201184 1106 34-35 0.22526712351019285 1106 36-37 0.19071535046218457 1106 38-39 0.07750668654643533 1106 40-41 0.28747791029937275 1106 42-43 0.1980381199808363 1106 44-45 0.37205642184557774 1106 46-47 0.23841634876094275 1106 48-49 0.36493308577836103 1106 50-51 0.4424974287826231 1106 52-53 0.49877318061655984 1106 54-55 0.5116607834739995 1106 56-57 0.5422157369353613 1106 58-59 0.4779226647586725 1106 60-61 0.4233088845621822 1106 62-63 0.5309057097133021 1106 64-65 0.515662023229595 1106 66-67 0.5202656033052051 1106 68-69 0.513060310622599 1106 70-71 0.3832717313300904 1106 72-73 0.46977334903417756 1106 74-75 0.5570680109838761 1106 76-77 0.4834776756860961 1106 78-79 0.4023345803514857 1106 80-81 0.6110744441373939 1106 82-83 0.5667814167926295 1106 84-85 0.5597622985694812 1106 86-87 0.5265429887754252 1106 88-89 0.47182707341411145 1106 90-91 0.500057903244354 1106 92-93 0.5374687017919371 1106 94-95 0.5782386380131541 1106 96-97 0.5264294910093952 1106 98-99 0.7687798607974052 1106 100-101 0.8049036914572838 1107 1 0.21529072292414497 1107 2 0.2292421000563749 1107 3 0.1989426085356527 1107 4 0.1686435369004755 1107 5 0.17438054827159277 1107 6 0.10444819430104957 1107 7 0.18606360646163722 1107 8 0.25304475229492596 1107 9 0.2075537471643969 1107 10-11 0.16904127625524978 1107 12-13 0.16000626523847217 1107 14-15 0.29263680030232564 1107 16-17 0.31152194569744296 1107 18-19 0.2832055884777702 1107 20-21 0.2654079969049832 1107 22-23 0.3544597452224636 1107 24-25 0.20463322700070563 1107 26-27 0.28418657310387374 1107 28-29 0.32776235583692426 1107 30-31 0.3312560906843487 1107 32-33 0.3496644413656824 1107 34-35 0.2859495811235746 1107 36-37 0.39862997329129257 1107 38-39 0.47168672101548026 1107 40-41 0.3541887680482816 1107 42-43 0.4265133803399621 1107 44-45 0.42434331035147466 1107 46-47 0.4593187306602715 1107 48-49 0.40160431885686165 1107 50-51 0.3678034520271325 1107 52-53 0.30046054344007445 1107 54-55 0.3475402596623738 1107 56-57 0.36043203755255604 1107 58-59 0.376451101720086 1107 60-61 0.26582365174883904 1107 62-63 0.42727762186143536 1107 64-65 0.3866085962111754 1107 66-67 0.2966535715465497 1107 68-69 0.2685687290237624 1107 70-71 0.32224973890149755 1107 72-73 0.25958067591560763 1107 74-75 0.2770048177362767 1107 76-77 0.1922127796087949 1107 78-79 0.1120919713099866 1107 80-81 0.22920899125245597 1107 82-83 0.3489422134105311 1107 84-85 0.40446967995324457 1107 86-87 0.49870481651858967 1107 88-89 0.4802226425216034 1107 90-91 0.44072695622946867 1107 92-93 0.4984339823316546 1107 94-95 0.5019729317660122 1107 96-97 0.33022108586555277 1107 98-99 0.5266429273638984 1107 100-101 0.8064872052375343 1108 1 -0.1378093010918917 1108 2 -0.010823357616750684 1108 3 -0.13645815215846824 1108 4 -0.047269044712173525 1108 5 -0.0051058435696518245 1108 6 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2316 60-61 -0.23042867904379705 2316 62-63 -0.15297729324864662 2316 64-65 -0.2601710253008207 2316 66-67 -0.06504046489560267 2316 68-69 0.05673341383516828 2316 70-71 0.016131817150700556 2316 72-73 -0.07256545251515689 2316 74-75 0.028426805697932878 2316 76-77 -0.27189608067450166 2316 78-79 -0.11145557685972562 2316 80-81 0.01223264266401003 2316 82-83 -0.012537144762781338 2316 84-85 0.015325448869468516 2316 86-87 0.02263122071891388 2316 88-89 0.06442457094582466 2316 90-91 0.01337101557062681 2316 92-93 0.0013096488565622622 2316 94-95 0.10410254019284793 2316 96-97 0.06064856141267683 2316 98-99 -0.029917640531675005 2316 100-101 0.30010001560097876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 7.0 6 19.0 7 41.0 8 99.0 9 205.0 10 415.0 11 655.0 12 907.0 13 1327.0 14 1558.0 15 1878.0 16 2340.0 17 3357.0 18 3392.0 19 2234.0 20 1852.0 21 2142.0 22 2644.0 23 2890.0 24 3459.0 25 4166.0 26 4316.0 27 4627.0 28 5900.0 29 7927.0 30 10057.0 31 13193.0 32 17436.0 33 23701.0 34 33627.0 35 53468.0 36 98276.0 37 207716.0 38 305253.0 39 89410.0 40 112.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.637706056026154 19.886054093002002 14.342531896945626 24.13370795402622 2 16.185449721504753 21.483558238012403 38.50097956530142 23.830012475181416 3 18.01375587117406 25.537204876382752 30.859670566323267 25.589368686119922 4 13.657961679898422 18.524421266294393 36.6225632554073 31.195053798399886 5 14.279470419763499 36.691529176110905 34.821459947639376 14.207540456486214 6 31.033002126052047 36.16396956758561 18.328742993692128 14.474285312670215 7 26.34854954052677 32.2772257656423 23.13212710628503 18.242097587545903 8 24.87458928540052 33.99805404740569 21.660473002653173 19.466883664540617 9 25.393363554899583 15.991293729025003 21.3891158434804 37.226226872595014 10-11 23.926815929576723 25.98609939732574 29.266709714827893 20.82037495826964 12-13 24.705965684465763 24.17664900813522 28.72437975506475 22.393005552334262 14-15 22.59411721748088 26.120228660788168 26.051153818910805 25.23450030282015 16-17 22.143872962184066 28.37748944371537 27.209681581822874 22.268956012277684 18-19 22.133960021194643 27.366236276332007 28.641703478172538 21.85810022430081 20-21 22.86939056103175 26.845470279198075 28.234871646196822 22.05026751357335 22-23 22.653820304675556 26.802696659814103 27.968181698381738 22.5753013371286 24-25 22.550318029272752 26.896040886967825 28.217740235095672 22.33590084866375 26-27 22.57534559479203 27.000155940452707 28.150601139463475 22.273897325291784 28-29 22.38111632948572 26.710738228867914 28.403040092162605 22.505105349483753 30-31 22.651623969919 26.806430428900253 28.210547238767948 22.331398362412806 32-33 22.404548611778402 26.768723077725586 28.199031964924515 22.6276963455715 34-35 22.544001414591044 26.800514656338393 28.05711740831105 22.59836652075951 36-37 22.68682844114882 26.830341061842716 28.04524209065111 22.43758840635735 38-39 22.518471175302654 26.68552220055172 28.225811765326025 22.5701948588196 40-41 22.685282800063256 26.68552220055172 28.136036582151707 22.49315841723332 42-43 22.76270360023193 26.655761864600354 27.992615922548453 22.58891861261926 44-45 22.782415704672044 26.73466519072971 27.873794212218648 22.609124892379597 46-47 22.924134204838964 26.692111204821394 27.524137718974572 22.859616871365066 48-49 22.73601813293975 26.74927081686082 27.67469646653664 22.840014583662786 50-51 22.648933459842215 26.872430288334826 27.68298763024265 22.795648621580305 52-53 22.80997970586685 26.988836030432417 27.58980812819567 22.61137613550507 54-55 22.726518985117636 27.023153761003638 27.447375819232867 22.802951434645863 56-57 22.85456530142498 27.1343432080544 27.266727285505947 22.744364205014673 58-59 22.664674587761013 27.190943618346676 27.54191849058841 22.602463303303896 60-61 22.84185609024464 27.318396888176533 27.316365241713765 22.523381779865066 62-63 22.54146680321905 27.474979887811052 27.311391598733685 22.67216171023621 64-65 22.675043680614813 27.431975198415966 27.21200719975137 22.680973921217852 66-67 22.629786551749888 27.49019297760089 27.063592994293696 22.81642747635552 68-69 22.58607766963636 27.299682897030763 27.333177756118985 22.781061677213888 70-71 22.649672141769173 27.04899039871207 27.398866909654767 22.902470549863992 72-73 22.741023150281066 27.172133780608544 27.104702045630635 22.982141023479752 74-75 22.667940618470652 27.211775268312373 27.16965798237883 22.950626130838145 76-77 22.742542497032154 27.16795538731354 27.165649197830426 22.92385291782388 78-79 22.710772753354217 27.13617008425694 27.15170916119184 23.001348001197 80-81 22.69088357182511 27.057811857358505 27.11459082080746 23.136713750008923 82-83 22.67876382319056 27.109708001058742 27.16088771544079 23.050640460309914 84-85 22.81831729433457 27.09086276744934 26.991422073308275 23.099397864907814 86-87 22.63924884691412 27.030035141664836 27.19816604436635 23.13254996705469 88-89 22.70929022657951 26.960573024052625 27.056058730023636 23.27407801934423 90-91 22.694891764623197 26.962205471509144 27.109469716936374 23.23343304693128 92-93 22.724816825681305 26.847282255372235 27.1504862685151 23.27741465043136 94-95 22.656840264965826 26.836739848540752 27.105681039481315 23.400738847012107 96-97 22.604976680390113 26.910236798091823 27.07864095048687 23.406145571031193 98-99 22.620051657793475 26.82636216681602 27.112928944177956 23.44065723121255 100-101 22.552893347946313 27.02079713948733 26.91334122551356 23.5129682870528 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 386.0 1 233.0 2 87.0 3 86.0 4 68.5 5 53.0 6 91.5 7 189.0 8 288.0 9 357.0 10 322.0 11 250.0 12 255.0 13 273.0 14 302.5 15 346.5 16 396.5 17 421.0 18 468.5 19 527.5 20 564.5 21 646.5 22 743.5 23 856.0 24 1055.5 25 1342.5 26 1676.0 27 2219.0 28 3115.5 29 4436.5 30 6351.5 31 8943.0 32 12611.5 33 17047.5 34 21014.0 35 24746.5 36 28458.5 37 31098.0 38 32065.5 39 32071.0 40 32583.5 41 32834.0 42 32515.5 43 32978.0 44 33726.0 45 34219.5 46 34683.0 47 34932.0 48 35358.0 49 35444.0 50 34226.0 51 32508.0 52 31376.0 53 30208.0 54 28511.5 55 26818.0 56 24991.0 57 22454.0 58 19847.5 59 17419.0 60 14615.0 61 12055.0 62 9683.0 63 7359.5 64 5564.5 65 4288.0 66 3275.0 67 2524.0 68 1921.0 69 1388.5 70 976.0 71 769.0 72 616.0 73 455.5 74 343.5 75 254.0 76 208.5 77 141.0 78 81.5 79 60.0 80 37.5 81 27.0 82 17.0 83 12.5 84 12.0 85 9.0 86 4.5 87 2.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019327745857712648 2 0.0 3 0.0016472510674186915 4 0.018888478906401 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6472510674186917E-4 16-17 0.001427617591762866 18-19 5.490836891395639E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.392669513116511E-4 28-29 0.004777028095514206 30-31 0.0 32-33 5.490836891395639E-5 34-35 0.010707131938221497 36-37 0.004392669513116511 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.6472510674186917E-4 60-61 0.0018119761741605608 62-63 0.009938414773426106 64-65 0.002306151494386168 66-67 0.00395340256180486 68-69 0.002251243125472212 70-71 1.0981673782791277E-4 72-73 0.006314462425104985 74-75 0.006094828949449159 76-77 0.0016472510674186915 78-79 5.490836891395639E-5 80-81 0.00653409590076081 82-83 0.009993323142340063 84-85 0.0016472510674186915 86-87 8.785339026233022E-4 88-89 5.490836891395639E-5 90-91 0.0 92-93 0.0 94-95 0.0 96-97 1.0981673782791277E-4 98-99 0.0 100-101 2.1963347565582555E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 910608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.48147743371503 #Duplication Level Percentage of deduplicated Percentage of total 1 66.33791796025716 40.12215288112932 2 20.321897211067018 24.581967351628524 3 7.2511202381812385 13.156753951641386 4 2.918522164695503 7.060661297753128 5 1.3188211011929496 3.9882124335454288 6 0.673302134614206 2.4433384716444753 7 0.3642717225983978 1.5422184379052848 8 0.2122527761395971 1.0269889192264328 9 0.16697716255247672 0.9089122939977048 >10 0.4267212216788462 3.767016676568249 >50 0.0047363890174997635 0.19863709786369219 >100 0.0021852115712610812 0.24979149061558764 >500 5.463027573554705E-4 0.23789662481499252 >1k 7.284036764739606E-4 0.7154520716658294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2456 0.2697099081053538 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1518 0.1667018080227716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.16011280375309683 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1064 0.11684500904889919 RNA PCR Primer, Index 27 (95% over 23bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.6472510674186915E-4 0.0 22-23 0.0 0.0 0.0 3.2945021348373835E-4 0.0 24-25 0.0 0.0 0.0 4.392669513116511E-4 0.0 26-27 0.0 0.0 0.0 0.0010432590093651713 0.0 28-29 0.0 0.0 0.0 0.0018119761741605608 0.0 30-31 0.0 0.0 0.0 0.0036239523483211217 0.0 32-33 0.0 0.0 0.0 0.007302813065556199 0.0 34-35 0.0 0.0 0.0 0.01504489308242405 0.0 36-37 0.0 0.0 0.0 0.02344587352625938 0.0 38-39 0.0 0.0 0.0 0.032780296241631965 0.0 40-41 0.0 0.0 0.0 0.04024783441393003 1.0981673782791277E-4 42-43 0.0 0.0 0.0 0.053370934584365606 1.0981673782791277E-4 44-45 0.0 0.0 0.0 0.07072197916117583 1.0981673782791277E-4 46-47 0.0 0.0 0.0 0.08840247395146979 1.0981673782791277E-4 48-49 0.0 0.0 0.0 0.11563702493279215 1.0981673782791277E-4 50-51 0.0 0.0 0.0 0.1396319821481911 1.0981673782791277E-4 52-53 0.0 0.0 0.0 0.1658232741201483 1.0981673782791277E-4 54-55 0.0 0.0 0.0 0.20211770597227346 1.0981673782791277E-4 56-57 0.0 0.0 0.0 0.23852195456222655 1.0981673782791277E-4 58-59 0.0 0.0 0.0 0.2760243705304588 1.0981673782791277E-4 60-61 0.0 0.0 0.0 0.31797436438072146 1.0981673782791277E-4 62-63 0.0 0.0 0.0 0.35190773636954653 1.0981673782791277E-4 64-65 0.0 0.0 0.0 0.39616388171419537 1.0981673782791277E-4 66-67 0.0 0.0 0.0 0.467105494351027 1.0981673782791277E-4 68-69 0.0 0.0 0.0 0.5533116335459385 1.0981673782791277E-4 70-71 0.0 0.0 0.0 0.6397923145854199 1.0981673782791277E-4 72-73 0.0 0.0 0.0 0.7308303902447595 1.0981673782791277E-4 74-75 0.0 0.0 0.0 0.8307087132992461 1.0981673782791277E-4 76-77 0.0 0.0 0.0 0.9470046386590059 1.0981673782791277E-4 78-79 0.0 0.0 0.0 1.071481910986945 1.0981673782791277E-4 80-81 0.0 0.0 0.0 1.208752833271836 1.0981673782791277E-4 82-83 0.0 0.0 0.0 1.3545345527383903 1.0981673782791277E-4 84-85 0.0 0.0 0.0 1.5147571732293148 1.0981673782791277E-4 86-87 0.0 0.0 0.0 1.7166003373570187 1.0981673782791277E-4 88-89 0.0 0.0 0.0 1.950290355454817 1.0981673782791277E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1185 0.0 53.317944 1 GTATCAA 1980 0.0 37.66817 1 TCAACGC 2335 0.0 31.12411 4 ATCAACG 2400 0.0 30.281162 3 CAACGCA 2455 0.0 29.792976 5 TATCAAC 2485 0.0 29.245388 2 AACGCAG 2520 0.0 29.024508 6 ACGCAGA 2885 0.0 24.85855 7 CGCAGAG 2925 0.0 24.518604 8 GTGGTAT 495 0.0 22.073069 1 GCAGAGT 3355 0.0 20.243618 9 GTACATG 2210 0.0 20.205782 1 GAGTACT 1770 0.0 20.125956 12-13 CAGAGTA 3020 0.0 19.974012 10-11 TACATGG 2180 0.0 19.174255 2 AGAGTAC 2830 0.0 18.377913 10-11 GTACTTT 1960 0.0 17.811472 14-15 ACATGGG 2230 0.0 17.679321 3 ATAGGGG 195 2.2747097E-4 17.051233 3 CATGGGG 1625 0.0 16.953796 4 >>END_MODULE