FastQCFastQC Report
Fri 10 Feb 2017
SRR2031739_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031739_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601091
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46190.7684360604301179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.6622957256056072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33840.562976321388941No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.4332122756787242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17130.2849818080789764No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.1460677335045775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.061.2110141
GTGGTAT7150.042.5287441
GTATCAA34200.041.2609251
TGGTATC7600.037.5099332
ATCAACG40950.034.2276233
TCAACGC41100.034.1027074
CAACGCA41800.033.6452755
TATCAAC42050.033.4452442
AACGCAG43000.032.6954356
GGTATAC650.005834319629.2385143
CATTGCG650.005843830329.2287679
ACGCAGA48350.028.9794037
CGCAGAG49050.028.2753358
TTCGAGT350.00839451627.14134-35
GCAGAGT52550.025.5786519
GAGTACT30200.025.09147612-13
GTACATG34750.024.0639271
TACATGG35000.023.892042
ACATGGG33950.023.651333
CAGAGTA50650.023.63509610-11
Produced by FastQC (version 0.11.5)