##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031739_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601091 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.037185717304034 33.0 31.0 34.0 31.0 34.0 2 32.3265994666365 34.0 31.0 34.0 31.0 34.0 3 32.41960535093688 34.0 31.0 34.0 31.0 34.0 4 35.842438166600395 37.0 37.0 37.0 35.0 37.0 5 35.85568907203734 37.0 37.0 37.0 35.0 37.0 6 35.897155339208204 37.0 37.0 37.0 35.0 37.0 7 35.892054614026826 37.0 37.0 37.0 35.0 37.0 8 35.888898685889494 37.0 37.0 37.0 35.0 37.0 9 37.66685410362158 39.0 39.0 39.0 35.0 39.0 10-11 37.61347200340714 39.0 39.0 39.0 35.0 39.0 12-13 37.56782750032857 39.0 38.0 39.0 35.0 39.0 14-15 38.935886579569484 41.0 39.0 41.0 36.0 41.0 16-17 38.85412108981835 41.0 39.0 41.0 36.0 41.0 18-19 38.86477421887867 41.0 39.0 41.0 36.0 41.0 20-21 38.8837954652457 41.0 39.0 41.0 35.5 41.0 22-23 38.77448672497176 41.0 39.0 41.0 35.0 41.0 24-25 38.690051922254696 41.0 39.0 41.0 35.0 41.0 26-27 38.565428529124546 41.0 39.0 41.0 35.0 41.0 28-29 38.43693301014322 40.0 38.0 41.0 35.0 41.0 30-31 38.22472221344189 40.0 38.0 41.0 35.0 41.0 32-33 37.96360201699909 40.0 38.0 41.0 34.0 41.0 34-35 37.77020201600091 40.0 38.0 41.0 34.0 41.0 36-37 37.58747510776239 40.0 37.5 41.0 33.5 41.0 38-39 37.384550758537394 40.0 37.0 41.0 33.0 41.0 40-41 37.25040384900123 40.0 37.0 41.0 33.0 41.0 42-43 37.14207249817415 40.0 37.0 41.0 33.0 41.0 44-45 36.915694961328654 40.0 37.0 41.0 31.5 41.0 46-47 36.67619212398788 40.0 37.0 41.0 31.0 41.0 48-49 36.568850639919745 40.0 37.0 41.0 31.0 41.0 50-51 35.99181405144978 39.5 36.5 40.5 29.5 41.0 52-53 35.98785707987643 39.5 36.5 40.5 30.0 41.0 54-55 36.18982566699552 40.0 37.0 41.0 30.0 41.0 56-57 36.054801186509195 40.0 36.0 41.0 29.0 41.0 58-59 35.88117023878248 40.0 36.0 41.0 28.5 41.0 60-61 35.648188044738646 39.5 35.0 41.0 28.0 41.0 62-63 35.373990793407316 39.0 35.0 41.0 27.5 41.0 64-65 35.10027350268096 39.0 35.0 41.0 26.5 41.0 66-67 34.803167074536134 38.5 35.0 41.0 26.0 41.0 68-69 34.467192155597075 37.5 35.0 40.0 26.0 41.0 70-71 34.08539805121022 37.0 35.0 40.0 25.0 41.0 72-73 33.69605683665202 36.5 35.0 39.0 24.0 41.0 74-75 33.277475457127125 36.0 34.0 39.0 22.5 41.0 76-77 32.8764113919523 35.5 34.0 38.5 21.5 40.0 78-79 32.468978074867195 35.0 34.0 37.0 20.0 39.0 80-81 32.09602206654234 35.0 34.0 37.0 18.5 39.0 82-83 31.751296392725894 35.0 34.0 36.0 16.0 38.5 84-85 31.410457817535114 35.0 33.5 36.0 9.0 37.0 86-87 31.12470907067316 35.0 33.0 36.0 4.5 37.0 88-89 30.838701627540587 35.0 33.0 35.0 2.0 36.5 90-91 30.588415897093785 35.0 33.0 35.0 2.0 36.0 92-93 30.361708959209174 35.0 33.0 35.0 2.0 36.0 94-95 30.108863716142814 35.0 32.5 35.0 2.0 36.0 96-97 29.8547990237751 35.0 32.0 35.0 2.0 35.0 98-99 29.62199733484614 35.0 32.0 35.0 2.0 35.0 100-101 29.079182686149018 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.023384348206420214 1101 2 0.1722638686541913 1101 3 0.3324748785579459 1101 4 0.23268688567240048 1101 5 0.21768421979006547 1101 6 0.2049185501882178 1101 7 0.35362551943258325 1101 8 0.31256752272478394 1101 9 0.41135854545227346 1101 10-11 0.2986894896073693 1101 12-13 0.35920299940223543 1101 14-15 0.26000417235442086 1101 16-17 0.3045542978719098 1101 18-19 0.560764321187726 1101 20-21 0.3392683647952879 1101 22-23 0.4089750535922363 1101 24-25 0.4106561573654517 1101 26-27 0.0619089553158787 1101 28-29 0.2270594917170996 1101 30-31 0.22391679470141668 1101 32-33 0.3178534268043691 1101 34-35 0.1538928516891076 1101 36-37 0.18631862508043184 1101 38-39 0.18474351570878156 1101 40-41 0.15857448519014383 1101 42-43 0.2869571656923924 1101 44-45 0.10943379718789714 1101 46-47 -0.16944940653896623 1101 48-49 0.05938959822776013 1101 50-51 -0.019397867063041474 1101 52-53 -0.2239117391241905 1101 54-55 0.018604141206267855 1101 56-57 0.014526855485975432 1101 58-59 -0.0701364132515323 1101 60-61 -0.08131455645924746 1101 62-63 0.19241124365885298 1101 64-65 0.1465595498607044 1101 66-67 -0.31490933998091464 1101 68-69 -0.30379418295787985 1101 70-71 -0.4268190631496154 1101 72-73 0.12359081429284657 1101 74-75 0.1335986131117224 1101 76-77 0.06724633936616442 1101 78-79 -0.001752228275883283 1101 80-81 -0.08544513336646986 1101 82-83 -0.07583616793106174 1101 84-85 -0.25746643520773205 1101 86-87 -0.2590894021916732 1101 88-89 -0.4854819304175102 1101 90-91 -0.8515850504921829 1101 92-93 -0.7223150309112434 1101 94-95 -0.44678146646964834 1101 96-97 -0.6741186593176103 1101 98-99 -0.7107013594746654 1101 100-101 -0.611841126498458 1102 1 0.09658159579550585 1102 2 0.12679537045758593 1102 3 0.291956115608059 1102 4 0.3131104909214173 1102 5 0.3543327246374801 1102 6 0.1818965257848717 1102 7 0.20654036352232197 1102 8 0.1252705641900036 1102 9 0.2567272815431352 1102 10-11 0.20016801517460436 1102 12-13 0.18947951949535025 1102 14-15 0.3802494848144491 1102 16-17 0.21602821903426417 1102 18-19 0.2449980714755 1102 20-21 0.15993958470677683 1102 22-23 0.20403427135500607 1102 24-25 0.028452182229244727 1102 26-27 0.12734905968239474 1102 28-29 0.18050384236811112 1102 30-31 -0.015032633584972643 1102 32-33 0.2727366539805942 1102 34-35 0.5409954020178134 1102 36-37 0.14189251298874694 1102 38-39 0.0615565001305427 1102 40-41 0.25081606296622283 1102 42-43 0.1785681958000822 1102 44-45 0.1487311138430485 1102 46-47 0.1768210255076994 1102 48-49 0.23816840773329062 1102 50-51 0.08258330603700159 1102 52-53 0.1883999279261772 1102 54-55 0.4775993577411981 1102 56-57 0.32420505874487304 1102 58-59 0.3592946869454323 1102 60-61 -0.018963240366844047 1102 62-63 -0.05077807412182267 1102 64-65 0.21943065210408008 1102 66-67 0.09398099844284502 1102 68-69 -0.013550405299476154 1102 70-71 -0.1708525218250827 1102 72-73 -0.1931922414971936 1102 74-75 -0.41042461355289817 1102 76-77 0.09197583057942182 1102 78-79 -0.21959173918576624 1102 80-81 -0.23912354126558455 1102 82-83 0.03540497498455153 1102 84-85 0.06534071696784949 1102 86-87 -0.10576911467216732 1102 88-89 -0.1848270817233697 1102 90-91 -0.35939974877576475 1102 92-93 -5.017531049489321E-5 1102 94-95 -0.4603196958201714 1102 96-97 -0.7429097472361654 1102 98-99 -0.5884799899200139 1102 100-101 -0.8602807448444558 1103 1 -0.12484958346700026 1103 2 0.05960591631606604 1103 3 0.10930675608097573 1103 4 -0.03872625645493599 1103 5 -0.028583854639009587 1103 6 0.007240454691988418 1103 7 0.02162274333169023 1103 8 0.08435642407053479 1103 9 4.915067223834058E-4 1103 10-11 0.04076055943287571 1103 12-13 0.1365133025968106 1103 14-15 0.19990528402677654 1103 16-17 0.21884218281977752 1103 18-19 0.2435085544130544 1103 20-21 0.009010918397436285 1103 22-23 0.10341710248611236 1103 24-25 0.13631496799513343 1103 26-27 0.32726477670910725 1103 28-29 0.25195405326974907 1103 30-31 0.24172942431781763 1103 32-33 0.19004806597285295 1103 34-35 0.1897296038153229 1103 36-37 0.1679639165915603 1103 38-39 0.4346562492236288 1103 40-41 0.6936823054992161 1103 42-43 0.5457526007312126 1103 44-45 0.3826785088389002 1103 46-47 0.5692398303207611 1103 48-49 0.4957363972930793 1103 50-51 0.26201088094009606 1103 52-53 0.30816331415969955 1103 54-55 0.3668857101139338 1103 56-57 0.39704768650931754 1103 58-59 0.5757485099744954 1103 60-61 0.6907926108911795 1103 62-63 0.6867173621687925 1103 64-65 0.4973605535279546 1103 66-67 0.23995561256665354 1103 68-69 0.3075249988315534 1103 70-71 0.3319517319404568 1103 72-73 0.49056794431374584 1103 74-75 0.30126935768991103 1103 76-77 0.1872408364442748 1103 78-79 0.3181297463696069 1103 80-81 0.46628027904467473 1103 82-83 0.3309488526227611 1103 84-85 0.30592393960695574 1103 86-87 0.43861538658236654 1103 88-89 0.25600394062521303 1103 90-91 0.15926679360264728 1103 92-93 -0.2948386133955232 1103 94-95 0.0856611327164103 1103 96-97 0.15739747779548807 1103 98-99 -0.02427345391137692 1103 100-101 -0.24041443239931581 1104 1 -0.12636193433240095 1104 2 0.09024621635503394 1104 3 0.004722152027298421 1104 4 -0.0169491190526756 1104 5 0.0419853684845819 1104 6 0.08959385855402502 1104 7 0.16471687734099305 1104 8 0.15051015471476603 1104 9 0.03575466649885328 1104 10-11 0.07099327555067703 1104 12-13 -0.04536744692858008 1104 14-15 -0.001668204426799491 1104 16-17 0.08202269601799372 1104 18-19 0.20592681417272019 1104 20-21 0.3349278928376407 1104 22-23 0.21412082830368462 1104 24-25 0.34899869359819036 1104 26-27 0.3154341171024626 1104 28-29 0.11735661389784724 1104 30-31 0.06975939961251498 1104 32-33 0.12255181109704694 1104 34-35 0.02377680489364309 1104 36-37 -0.002713638047922018 1104 38-39 0.07015466357916722 1104 40-41 0.23771745782477183 1104 42-43 0.1825935393183329 1104 44-45 0.030663649902258783 1104 46-47 -0.33608271514808763 1104 48-49 -0.4479884192047692 1104 50-51 -0.7897433388048469 1104 52-53 -0.6151840939283417 1104 54-55 -0.6165999904834933 1104 56-57 -0.5477801092839911 1104 58-59 -0.585302556000471 1104 60-61 -0.5315050898349654 1104 62-63 -0.6629717657088747 1104 64-65 -0.5179309941111043 1104 66-67 -0.8102254762544661 1104 68-69 -0.6676677990890525 1104 70-71 -0.597319944194787 1104 72-73 -0.8255813172085382 1104 74-75 -0.7423957513387833 1104 76-77 -0.7417787812134051 1104 78-79 -0.9253426541209215 1104 80-81 -0.900001804781752 1104 82-83 -0.6086035916294108 1104 84-85 -0.6816769284752908 1104 86-87 -0.9287857653785032 1104 88-89 -0.6986791628760791 1104 90-91 -0.46631910828403633 1104 92-93 -0.7544466892776391 1104 94-95 -0.699088078036656 1104 96-97 -0.7911897433759911 1104 98-99 -0.8392947090553342 1104 100-101 -0.7077612398373674 1105 1 0.21099505072017166 1105 2 0.3888712984790601 1105 3 0.33651002184072354 1105 4 0.2986215961156802 1105 5 0.3241345840086609 1105 6 0.3401427704319673 1105 7 0.45001148557960846 1105 8 0.3953231502247547 1105 9 0.443862563000458 1105 10-11 0.5159948584593437 1105 12-13 0.5217700074138776 1105 14-15 0.6900577787637374 1105 16-17 0.5527507999657288 1105 18-19 0.676164727048068 1105 20-21 0.6499216122645279 1105 22-23 0.5326803473717803 1105 24-25 0.7733322550065367 1105 26-27 0.786410720691066 1105 28-29 0.6770424831189601 1105 30-31 0.7758047490949167 1105 32-33 0.7989797479032461 1105 34-35 0.6801154179933135 1105 36-37 0.8763163963675709 1105 38-39 0.9792327299115513 1105 40-41 0.8970584912428663 1105 42-43 0.8407805812535045 1105 44-45 0.9314476152076239 1105 46-47 0.966548998171838 1105 48-49 0.9963170242990032 1105 50-51 0.790481195939158 1105 52-53 1.1144244680757254 1105 54-55 0.9612910305096136 1105 56-57 1.1988472740147884 1105 58-59 0.9917748839766602 1105 60-61 1.071176323413134 1105 62-63 1.0608174793628322 1105 64-65 1.1307943388954413 1105 66-67 1.0801300143961612 1105 68-69 1.1598836624632796 1105 70-71 1.1190806916919698 1105 72-73 0.9160624568542275 1105 74-75 1.1790936806182657 1105 76-77 1.1452219642855184 1105 78-79 1.3534518162514502 1105 80-81 1.0669460409306097 1105 82-83 1.2002133798748247 1105 84-85 1.1471153292290523 1105 86-87 0.9673243642157594 1105 88-89 0.9434983747988532 1105 90-91 1.001923999255034 1105 92-93 0.7552805398151996 1105 94-95 0.8049457859965976 1105 96-97 0.8384266480688147 1105 98-99 0.8779603571034365 1105 100-101 0.8462414766381592 1106 1 -0.24182579721740893 1106 2 -0.14307982926604268 1106 3 -0.06003248395147409 1106 4 0.015338758831767052 1106 5 0.014673010512161966 1106 6 0.09391017176664462 1106 7 0.100697637780641 1106 8 0.2014678263569536 1106 9 0.15783709714964544 1106 10-11 -0.06806621205689822 1106 12-13 -0.06665253560677797 1106 14-15 -0.06992756748074669 1106 16-17 -0.07766267356839762 1106 18-19 0.018889335456158562 1106 20-21 0.05195927637668518 1106 22-23 0.13313778217246863 1106 24-25 0.07885810700361162 1106 26-27 -0.04112051755127055 1106 28-29 0.1347941217652746 1106 30-31 0.21626891670219806 1106 32-33 0.03438539950030872 1106 34-35 -0.045522713454836605 1106 36-37 -0.09157900750180659 1106 38-39 0.02553067640554474 1106 40-41 -0.14635325948486866 1106 42-43 -0.18682887635952028 1106 44-45 -0.20308475176993568 1106 46-47 -0.39469082835937996 1106 48-49 -0.5009601495064189 1106 50-51 -0.22921226333726707 1106 52-53 -0.1940222338391564 1106 54-55 -0.21829173350550946 1106 56-57 -0.17177887699810412 1106 58-59 -0.023472942712984946 1106 60-61 -0.20368060444682357 1106 62-63 -0.37103760687649867 1106 64-65 -0.2886446598966188 1106 66-67 -0.08498216604305497 1106 68-69 0.01703429910956089 1106 70-71 0.06674302163909829 1106 72-73 -0.3949238993112445 1106 74-75 -0.17091820080512576 1106 76-77 -0.2803953746716843 1106 78-79 -0.3670525501716426 1106 80-81 -0.46237137129537587 1106 82-83 -0.43773489632866003 1106 84-85 -0.27837824490113405 1106 86-87 -0.4287923905714628 1106 88-89 -0.25070349058462327 1106 90-91 -0.1393906802435687 1106 92-93 -0.2703140264138355 1106 94-95 -0.12181513996233306 1106 96-97 0.005395300913423995 1106 98-99 -0.08662309999472129 1106 100-101 -0.10157927228756236 1107 1 0.27687031825290376 1107 2 0.3812417273135793 1107 3 0.4345496557919617 1107 4 0.4076042962585973 1107 5 0.23816147593610992 1107 6 0.39547671526867134 1107 7 0.3924732843950025 1107 8 0.31994113837865257 1107 9 0.3679365975717275 1107 10-11 0.33872998506187457 1107 12-13 0.3716405962347409 1107 14-15 0.5393879692541432 1107 16-17 0.5825793657058966 1107 18-19 0.5454043297130866 1107 20-21 0.2823636438778294 1107 22-23 0.4897097919702844 1107 24-25 0.6742864846900503 1107 26-27 0.5263155821428924 1107 28-29 0.3384793738376217 1107 30-31 0.44929572230964965 1107 32-33 0.48895287019680467 1107 34-35 0.37125466299995935 1107 36-37 0.496604798423931 1107 38-39 0.5489423369139743 1107 40-41 0.38876942079909327 1107 42-43 0.5280588223991387 1107 44-45 0.6060584928571799 1107 46-47 0.7048753276583852 1107 48-49 0.5988931600243106 1107 50-51 0.5703684233401916 1107 52-53 0.3441968608315449 1107 54-55 0.3657542940889016 1107 56-57 0.4192764640572406 1107 58-59 0.30743861400502226 1107 60-61 0.528931039455145 1107 62-63 0.4577773832288159 1107 64-65 0.4816532878206843 1107 66-67 0.5265025906029948 1107 68-69 0.5878435876607782 1107 70-71 0.5270743206800717 1107 72-73 0.36661067673946235 1107 74-75 0.6002861833024795 1107 76-77 0.41772671054717136 1107 78-79 0.5328000674229116 1107 80-81 0.5124386922243644 1107 82-83 0.6048952914628174 1107 84-85 0.6056913085232551 1107 86-87 0.5552688596188204 1107 88-89 0.5608234741681493 1107 90-91 0.26496644185548845 1107 92-93 0.4984651213975724 1107 94-95 0.5669554278404938 1107 96-97 0.5405871748149735 1107 98-99 0.6589692665653892 1107 100-101 0.512856592148303 1108 1 -0.12606779574048943 1108 2 -0.018136114275321802 1108 3 -0.007836017749490054 1108 4 0.004721154681988082 1108 5 0.05504512196548461 1108 6 0.13204889405190556 1108 7 -0.016825461587167467 1108 8 0.05861197001431151 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2316 6 -0.7799678604177416 2316 7 -0.5948465626588231 2316 8 -0.4462440310713305 2316 9 -0.6484411552026401 2316 10-11 -1.0161555620848546 2316 12-13 -0.5784313015397018 2316 14-15 -0.9063467186208101 2316 16-17 -0.580532874189899 2316 18-19 -0.39080390304895474 2316 20-21 -0.30963399376672385 2316 22-23 -0.4233300832824085 2316 24-25 -0.5608239064058296 2316 26-27 -0.3782027727986801 2316 28-29 -0.5561040159090069 2316 30-31 -0.3634747697232399 2316 32-33 -0.471217396805379 2316 34-35 -1.0215231121840986 2316 36-37 -0.6081511926682168 2316 38-39 -0.2907851518461584 2316 40-41 -0.5976248591260145 2316 42-43 -0.29979386186224843 2316 44-45 -0.15376909254477056 2316 46-47 -0.07665114612927226 2316 48-49 -0.004368851339272339 2316 50-51 -0.1387700639119558 2316 52-53 -0.2507642256652147 2316 54-55 -0.15268234949888893 2316 56-57 -0.20936452794530425 2316 58-59 -0.21712101212654744 2316 60-61 -0.2181930495673754 2316 62-63 -0.04687613019132897 2316 64-65 0.06535000059841423 2316 66-67 0.11143533817177342 2316 68-69 -0.13919931290086396 2316 70-71 -0.1550067930818173 2316 72-73 -0.08749496630209563 2316 74-75 -0.08189647439407821 2316 76-77 0.02330088897503657 2316 78-79 0.03428470977816289 2316 80-81 -0.0843630239848494 2316 82-83 -0.02174866982443291 2316 84-85 -0.004059010114605854 2316 86-87 0.1347637544099669 2316 88-89 0.1779153025659035 2316 90-91 -0.008508881801212453 2316 92-93 0.0802686817380085 2316 94-95 0.0708339392764401 2316 96-97 0.15993864755217757 2316 98-99 0.036326369738691966 2316 100-101 0.033178217971741475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5167.0 3 627.0 4 433.0 5 382.0 6 465.0 7 561.0 8 679.0 9 835.0 10 906.0 11 1114.0 12 1566.0 13 2005.0 14 2466.0 15 3301.0 16 4185.0 17 6289.0 18 5847.0 19 3059.0 20 2541.0 21 2254.0 22 2227.0 23 2337.0 24 2535.0 25 2873.0 26 3199.0 27 3842.0 28 4364.0 29 5294.0 30 6598.0 31 8444.0 32 10934.0 33 14753.0 34 21786.0 35 38235.0 36 57892.0 37 118693.0 38 188465.0 39 63866.0 40 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.593006014361166 20.6584142745281 15.243823201106244 21.5047565100045 2 15.9391338249456 22.682363604253233 40.28360451981166 21.0948980509895 3 18.206838643466902 28.255552815892415 31.5800785024557 21.95753003818498 4 12.877552210457917 20.205426388877317 38.42872970518897 28.488291695475787 5 12.902000569735073 38.01465651805243 34.85929042995007 14.224052482262413 6 27.19118977070019 37.70763483251803 20.265892507074533 14.83528288970724 7 24.047419558485732 35.104931680722515 24.133536994684704 16.71411176610704 8 23.717451191360514 34.41904311415942 22.64850784302622 19.214997851453848 9 25.925093183839476 17.41430251188323 22.602285710478892 34.058318593798404 10-11 22.982501132397218 27.999686925474943 29.153316591617596 19.864495350510243 12-13 24.763884412665337 26.892484857872617 28.748847632681514 19.59478309678053 14-15 22.39478653287611 27.941091952826252 28.234449429985265 21.429672084312372 16-17 20.000333200053312 30.425496468079434 28.99398573903772 20.580184592829536 18-19 19.82630657406127 28.794191782465063 31.843739223294175 19.53576242017949 20-21 21.79483121659981 27.62220637818676 31.538191159028212 19.044771246185217 22-23 21.298429371698706 28.007539149105583 31.049933953322572 19.644097525873143 24-25 20.327874859263307 28.301816086287417 31.71447272872627 19.655836325723 26-27 20.12655033740372 28.359983910551996 31.78498767891391 19.728478073130372 28-29 20.08771301553883 28.341081371894187 31.82253521595957 19.74867039660741 30-31 20.577723149896908 28.14851455466619 31.553180575124994 19.720581720311912 32-33 20.328332456181258 28.255927438427253 31.247376871165233 20.168363234226252 34-35 20.660091514655836 28.363879861611984 31.2506558772431 19.72537274648908 36-37 20.47600526044447 28.254233716650607 31.1377335280042 20.13202749490072 38-39 20.252646972117063 28.48095362614777 31.265500005829416 20.00089939590575 40-41 20.502690336856325 28.290967780082767 31.138785738258246 20.067556144802666 42-43 20.482162486259618 28.377885480163883 30.67177975417208 20.468172279404417 44-45 20.743545387385034 28.602602084032608 30.399790780516934 20.25406174806543 46-47 20.71570099669434 28.520932913234205 30.118209098427627 20.645156991643827 48-49 20.5867041165745 28.824184510764766 29.91811164503885 20.67099972762189 50-51 20.802371350567103 28.869644014238766 29.6800275846905 20.64795705050364 52-53 20.950164259334052 29.037469448596614 29.4080418372063 20.604324454863036 54-55 21.017869335687045 28.958732326344368 29.311955634752778 20.711442703215816 56-57 20.889029917498263 29.14878129627501 29.143201227282038 20.818987558944684 58-59 20.60027364656955 29.13211732992233 29.5953844834234 20.672224540084724 60-61 20.971086424276745 29.04506920271148 29.395746240069286 20.58809813294249 62-63 20.780934980101048 29.060298113700924 29.390240144834724 20.768526761363304 64-65 20.836741391287255 28.834169313985004 29.364077208962957 20.965012085764783 66-67 20.72601950221707 29.168457312665115 29.065515989219477 21.04000719589834 68-69 20.892027968533316 29.02639020185152 29.23194809413352 20.84963373548164 70-71 20.768891883271277 28.709316628454978 29.430772164791335 21.091019323482406 72-73 20.644702970420717 28.93493090505097 29.24848780625931 21.171878318269 74-75 20.758732669969607 29.0664890295183 28.85565593904825 21.31912236146384 76-77 20.696872527381004 29.010619247907382 28.99720984466747 21.29529838004414 78-79 20.738933077916126 29.044517552992126 28.87794111522925 21.33860825386249 80-81 20.638788919640827 29.174603359688568 28.806135358685776 21.38047236198483 82-83 20.730681388464557 29.19435471914361 28.778554996010254 21.29640889638158 84-85 20.8703676558773 28.86595018855856 28.82247511426782 21.441207041296323 86-87 20.474965287986855 29.107865862952725 28.837582761185516 21.579586087874908 88-89 20.901628080706967 28.846068962530573 28.715565997273345 21.536736959489115 90-91 20.638576092750533 28.98862273454158 28.86435567697228 21.508445495735607 92-93 20.773709020302324 28.882809955493656 28.784601121363295 21.558879902840726 94-95 20.749993545738228 28.86087693013657 28.650926040822583 21.738203483302616 96-97 20.723522603315764 28.822495899011603 28.770785974203328 21.683195523469312 98-99 20.6436403727908 28.71441799734863 28.727075297613098 21.91486633224747 100-101 20.77665891385044 28.71668677067436 28.690529346297645 21.816124969177558 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5980.0 1 3426.5 2 832.0 3 768.0 4 607.0 5 495.0 6 661.0 7 1087.0 8 1578.0 9 1655.0 10 1237.5 11 874.0 12 810.0 13 826.5 14 856.5 15 894.0 16 936.5 17 992.5 18 1087.0 19 1188.0 20 1266.5 21 1417.0 22 1556.0 23 1748.5 24 2080.5 25 2480.0 26 3020.5 27 3818.0 28 4851.0 29 6388.5 30 8332.0 31 10294.0 32 12452.0 33 14723.0 34 17236.5 35 19620.5 36 22200.5 37 24214.0 38 24346.0 39 23868.0 40 23593.0 41 23063.0 42 22327.5 43 21915.0 44 21662.0 45 21600.0 46 21490.0 47 21134.5 48 20718.0 49 20184.0 50 19084.5 51 17657.0 52 16449.0 53 15236.5 54 13769.0 55 11989.0 56 10321.5 57 8865.0 58 7737.5 59 6941.5 60 6019.0 61 4804.0 62 3579.0 63 2623.5 64 1889.5 65 1461.0 66 1118.0 67 751.0 68 563.0 69 472.5 70 378.5 71 302.0 72 266.5 73 249.0 74 232.0 75 214.0 76 185.5 77 144.0 78 108.5 79 78.0 80 54.0 81 37.5 82 31.0 83 19.0 84 12.0 85 13.0 86 7.0 87 5.0 88 5.5 89 3.0 90 3.5 91 1.5 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 1.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14323954276473944 2 0.1478977392774139 3 0.14224135779773778 4 0.1457350051822436 5 0.13508769886755914 6 0.11612218449452746 7 0.12444039255287467 8 0.11395945039935718 9 0.11063216717601829 10-11 0.09898667589433215 12-13 0.11587263825277705 14-15 0.13300814685297233 16-17 0.14157590115307 18-19 0.13941316705789972 20-21 0.13225950812772108 22-23 0.12610403416454413 24-25 0.1129612654323555 26-27 0.11603900241394399 28-29 0.12044765268486803 30-31 0.11063216717601829 32-33 0.11029943885368439 34-35 0.12477312087520857 36-37 0.12668630872862843 38-39 0.114458542882858 40-41 0.11670445905861176 42-43 0.11163035214301995 44-45 0.12718540121212926 46-47 0.12585448792279372 48-49 0.13683452255981207 50-51 0.12610403416454413 52-53 0.11121444174010259 54-55 0.10331214408467271 56-57 0.12310947926353914 58-59 0.11337717583527288 60-61 0.1132939937546894 62-63 0.11354353999643982 64-65 0.1327586006112219 66-67 0.12460675671404162 68-69 0.12868267866263178 70-71 0.11770264402561344 72-73 0.12003174228195067 74-75 0.10248032327883798 76-77 0.12743494745387968 78-79 0.12743494745387968 80-81 0.1276844936956301 82-83 0.1334240572558897 84-85 0.12427402839170776 86-87 0.1321763260471376 88-89 0.12003174228195067 90-91 0.12826676825971442 92-93 0.13966271329965013 94-95 0.11853446483144817 96-97 0.10414396489050744 98-99 0.10763761227501327 100-101 0.14623409766574444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 601091.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.56088176581834 #Duplication Level Percentage of deduplicated Percentage of total 1 65.68455447955 34.52438101840081 2 17.89762792356145 18.814302103578445 3 7.363025245490314 11.610212981008559 4 3.533434004976522 7.428816278515718 5 1.8861488173802172 4.956882249152985 6 1.1082982975673286 3.495188146781646 7 0.6913292410682965 2.543581215073056 8 0.44292446775698063 1.8624400464770139 9 0.3098891936332412 1.4659244340355417 >10 1.0422982062268082 8.572326058296607 >50 0.02963560778434833 1.0208623398142498 >100 0.008603879562342421 0.7389732274563448 >500 6.373244120247785E-4 0.2358282647910047 >1k 0.0015933110300619462 2.7302816366180167 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4619 0.7684360604301179 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3981 0.6622957256056072 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3384 0.562976321388941 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2604 0.4332122756787242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1713 0.2849818080789764 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 0.1460677335045775 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.327283223338895E-4 0.0 0.0 0.0 0.0 12-13 3.327283223338895E-4 0.0 0.0 0.0 0.0 14-15 3.327283223338895E-4 0.0 0.0 0.0 0.0 16-17 3.327283223338895E-4 0.0 0.0 0.0 0.0 18-19 3.327283223338895E-4 0.0 0.0 0.0 0.0 20-21 6.65456644667779E-4 0.0 0.0 0.0 0.0 22-23 0.001081367047585141 0.0 0.0 6.654566446677792E-4 0.0 24-25 0.0011645491281686135 0.0 0.0 0.001497277450502503 0.0 26-27 0.0011645491281686135 0.0 0.0 0.00382637570683973 0.0 28-29 0.0016636416116694477 0.0 0.0 0.00765275141367946 0.0 30-31 0.0016636416116694477 0.0 0.0 0.013475497054522525 0.0 32-33 0.0019963699340033374 0.0 0.0 0.0286146357207145 0.0 34-35 0.002828190739838061 0.0 0.0 0.05556562982975955 0.0 36-37 0.0029113728204215334 0.0 0.0 0.08700845629031212 0.0 38-39 0.002994554901005006 0.0 0.0 0.1150408174469423 0.0 40-41 0.002994554901005006 0.0 0.0 0.1383318000103146 0.0 42-43 0.002994554901005006 0.0 0.0 0.16761189237569685 0.0 44-45 0.002994554901005006 0.0 0.0 0.21144884884318682 0.0 46-47 0.002994554901005006 0.0 0.0 0.26019354806510164 0.0 48-49 0.002994554901005006 0.0 0.0 0.31376280796085787 0.0 50-51 0.002994554901005006 0.0 0.0 0.36783116034011487 0.0 52-53 0.002994554901005006 0.0 0.0 0.4243949751368761 0.0 54-55 0.002994554901005006 0.0 0.0 0.4974288418891648 0.0 56-57 0.002994554901005006 0.0 0.0 0.5752872693152951 0.0 58-59 0.002994554901005006 0.0 0.0 0.6515652372103392 0.0 60-61 0.002994554901005006 0.0 0.0 0.7271777484607156 0.0 62-63 0.002994554901005006 0.0 0.0 0.8032893521945929 0.0 64-65 0.002994554901005006 0.0 0.0 0.8939578200305778 0.0 66-67 0.002994554901005006 0.0 0.0 1.0281305160117187 0.0 68-69 0.002994554901005006 0.0 0.0 1.1862596512008996 0.0 70-71 0.002994554901005006 0.0 0.0 1.3467178846464178 0.0 72-73 0.002994554901005006 0.0 0.0 1.5124997712492785 0.0 74-75 0.002994554901005006 0.0 0.0 1.6956667126940845 0.0 76-77 0.002994554901005006 0.0 0.0 1.8937232465633325 0.0 78-79 0.002994554901005006 0.0 0.0 2.1133239393036996 0.0 80-81 0.002994554901005006 0.0 0.0 2.345734672453921 0.0 82-83 0.002994554901005006 0.0 0.0 2.608590047097694 0.0 84-85 0.002994554901005006 0.0 0.0 2.8872500170523265 0.0 86-87 0.002994554901005006 0.0 0.0 3.219811975225049 0.0 88-89 0.002994554901005006 0.0 0.0 3.5799072020709013 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1925 0.0 61.211014 1 GTGGTAT 715 0.0 42.528744 1 GTATCAA 3420 0.0 41.260925 1 TGGTATC 760 0.0 37.509933 2 ATCAACG 4095 0.0 34.227623 3 TCAACGC 4110 0.0 34.102707 4 CAACGCA 4180 0.0 33.645275 5 TATCAAC 4205 0.0 33.445244 2 AACGCAG 4300 0.0 32.695435 6 GGTATAC 65 0.0058343196 29.238514 3 CATTGCG 65 0.0058438303 29.228767 9 ACGCAGA 4835 0.0 28.979403 7 CGCAGAG 4905 0.0 28.275335 8 TTCGAGT 35 0.008394516 27.141 34-35 GCAGAGT 5255 0.0 25.578651 9 GAGTACT 3020 0.0 25.091476 12-13 GTACATG 3475 0.0 24.063927 1 TACATGG 3500 0.0 23.89204 2 ACATGGG 3395 0.0 23.65133 3 CAGAGTA 5065 0.0 23.635096 10-11 >>END_MODULE