FastQCFastQC Report
Fri 10 Feb 2017
SRR2031739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601091
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.8271626093220494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.7210222744975386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36590.608726465709851No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31720.5277071192215488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23010.3828039348451399No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT21800.36267387134393964No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.17518146170879284No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT9750.16220505713777114RNA PCR Primer, Index 27 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.12477312087520857No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT7270.12094674516836885RNA PCR Primer, Index 27 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.058.7034841
GTATCAA34600.039.3991851
TATCAAC43950.031.1254082
ATCAACG44650.030.2119183
TCAACGC44900.030.04374
CGGAGAT953.48877E-529.9991591
CAACGCA45850.029.4212025
GTGGTAT8900.029.353111
TGGTATC8950.029.1891252
AACGCAG46550.028.9787796
ACGCAGA52200.025.6602027
CGCAGAG52850.025.3446068
GCAGAGT56700.023.288599
GAGTACT34200.022.70769712-13
CGTCAGA2005.3787335E-721.3744012
CAGAGTA56250.021.02782610-11
GTACTTT38050.020.09799214-15
AGAGTAC53950.019.76707610-11
GTACATG34100.019.3616281
ACATGGG33050.019.258163
Produced by FastQC (version 0.11.5)