##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031739_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601091 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62794818089108 34.0 31.0 34.0 31.0 34.0 2 32.79166881553708 34.0 31.0 34.0 31.0 34.0 3 32.82905749711774 34.0 31.0 34.0 31.0 34.0 4 36.280104010873565 37.0 37.0 37.0 35.0 37.0 5 36.24879760302517 37.0 37.0 37.0 35.0 37.0 6 36.29174451123041 37.0 37.0 37.0 35.0 37.0 7 36.24207649091402 37.0 37.0 37.0 35.0 37.0 8 36.232693552224205 37.0 37.0 37.0 35.0 37.0 9 38.05389200636842 39.0 39.0 39.0 35.0 39.0 10-11 38.02084043846938 39.0 38.5 39.0 35.0 39.0 12-13 37.96482812086689 39.0 38.0 39.0 35.0 39.0 14-15 39.35267704889942 41.0 39.0 41.0 36.0 41.0 16-17 39.31117750889632 41.0 39.0 41.0 36.0 41.0 18-19 39.345188166184485 41.0 39.0 41.0 36.0 41.0 20-21 39.35958615251268 41.0 39.0 41.0 36.0 41.0 22-23 39.25283942032071 41.0 39.0 41.0 35.5 41.0 24-25 39.16342866554315 41.0 39.0 41.0 35.0 41.0 26-27 39.0020238200206 40.5 39.0 41.0 35.0 41.0 28-29 38.87588817666543 40.0 38.0 41.0 35.0 41.0 30-31 38.62253552290751 40.0 38.0 41.0 35.0 41.0 32-33 38.32892606943042 40.0 38.0 41.0 34.5 41.0 34-35 38.13937823058406 40.0 37.5 41.0 34.0 41.0 36-37 37.986325697772884 40.0 37.5 41.0 34.0 41.0 38-39 38.00380142108267 40.0 38.0 41.0 34.0 41.0 40-41 37.87860823070051 40.0 38.0 41.0 33.5 41.0 42-43 37.70680895238824 40.0 37.0 41.0 33.0 41.0 44-45 37.414020506046505 40.0 37.0 41.0 33.0 41.0 46-47 37.043813665484926 40.0 37.0 41.0 32.5 41.0 48-49 36.64798424864122 40.0 37.0 41.0 31.0 41.0 50-51 36.40678116957332 40.0 36.0 41.0 30.0 41.0 52-53 36.44175008442981 40.0 36.5 41.0 30.0 41.0 54-55 36.34945540691842 40.0 36.0 41.0 30.0 41.0 56-57 36.14273795481882 40.0 36.0 41.0 30.0 41.0 58-59 35.94677927302189 40.0 35.5 41.0 29.0 41.0 60-61 35.66639743399918 39.0 35.0 41.0 28.0 41.0 62-63 35.37856248055619 39.0 35.0 41.0 28.0 41.0 64-65 35.04041068656826 39.0 35.0 41.0 27.0 41.0 66-67 34.664651442127735 38.0 35.0 40.0 26.0 41.0 68-69 34.25297417529126 37.0 35.0 40.0 25.5 41.0 70-71 33.881594467393455 37.0 34.5 39.5 24.5 41.0 72-73 33.48912727024693 36.0 34.0 39.0 23.5 41.0 74-75 33.098277964567764 36.0 34.0 39.0 22.0 40.5 76-77 32.17016475042881 35.0 32.5 37.0 20.5 39.0 78-79 32.34523557997042 35.0 34.0 37.0 20.5 39.0 80-81 32.103828704805096 35.0 34.0 37.0 20.0 39.0 82-83 31.804785797824287 35.0 34.0 36.0 19.0 38.0 84-85 31.477089159544896 35.0 33.0 36.0 17.5 37.0 86-87 31.196271446419928 35.0 33.0 35.5 12.5 37.0 88-89 30.94401097338007 35.0 33.0 35.0 7.5 36.5 90-91 30.71655040584537 35.0 33.0 35.0 3.5 36.0 92-93 30.51302298653615 35.0 33.0 35.0 2.0 36.0 94-95 30.30003526920217 35.0 33.0 35.0 2.0 36.0 96-97 30.10992927859509 35.0 32.0 35.0 2.0 35.0 98-99 29.879222114455217 35.0 32.0 35.0 2.0 35.0 100-101 29.174694846537378 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2497694910891326 1101 2 0.23862238280035086 1101 3 0.27536471385054284 1101 4 0.136809847119153 1101 5 0.15965693714018414 1101 6 0.14563736355482604 1101 7 0.1361514189566364 1101 8 0.25816084059079714 1101 9 0.12266794436135342 1101 10-11 0.2003586627431062 1101 12-13 0.2375420120140106 1101 14-15 0.312963777437524 1101 16-17 0.3767675049032704 1101 18-19 0.3617955346089019 1101 20-21 0.19848223218235006 1101 22-23 -0.02438656644142867 1101 24-25 0.1861585235488903 1101 26-27 0.3567459849903898 1101 28-29 0.3259329093098984 1101 30-31 0.2711276849668067 1101 32-33 0.3700093512401139 1101 34-35 0.10480741981961472 1101 36-37 0.330952650365397 1101 38-39 0.4059110505146606 1101 40-41 0.10419377118524409 1101 42-43 0.4997208951380614 1101 44-45 0.5685212716437889 1101 46-47 0.7243106218119166 1101 48-49 0.735659349393103 1101 50-51 0.716345561869467 1101 52-53 0.6761886515890794 1101 54-55 0.6186423702403943 1101 56-57 0.4978376388259065 1101 58-59 0.4983660979610036 1101 60-61 0.4515136304837739 1101 62-63 0.5549095441474563 1101 64-65 0.6823043817927044 1101 66-67 0.8107808184642948 1101 68-69 0.8198694518598728 1101 70-71 0.8353118641020032 1101 72-73 0.5906512607067569 1101 74-75 0.4325067050109581 1101 76-77 0.5562257977010319 1101 78-79 0.6145154363767773 1101 80-81 0.6473445693218807 1101 82-83 0.502649946002844 1101 84-85 0.5272730555355665 1101 86-87 0.4798573228734426 1101 88-89 0.6453368891721851 1101 90-91 0.3197020134393149 1101 92-93 0.2730803710663956 1101 94-95 0.5434490075231722 1101 96-97 0.5409066693262901 1101 98-99 0.5873668158804861 1101 100-101 0.4361029197242523 1102 1 0.15810346392314045 1102 2 0.07676476123127429 1102 3 0.14608325681219725 1102 4 -0.0128333115025967 1102 5 0.028316287919096794 1102 6 0.030872571904147605 1102 7 0.16660444123683504 1102 8 -0.014100178005882924 1102 9 0.03307389939165972 1102 10-11 0.029835806988877778 1102 12-13 0.17904049000240008 1102 14-15 0.28630938918387727 1102 16-17 0.2419032069471143 1102 18-19 0.24557268141267485 1102 20-21 0.2585633208682978 1102 22-23 0.21369314092306269 1102 24-25 0.3087571985034785 1102 26-27 0.2968695365213634 1102 28-29 0.3397205439247841 1102 30-31 0.293228828394021 1102 32-33 0.5207140809871262 1102 34-35 0.15547505852730836 1102 36-37 0.2549032297530047 1102 38-39 0.001273391598743956 1102 40-41 -0.019389734813280768 1102 42-43 0.10610822790863494 1102 44-45 0.15569800405112488 1102 46-47 0.34181247636387724 1102 48-49 0.18432023497364014 1102 50-51 0.24543005269902807 1102 52-53 0.16336026799098846 1102 54-55 0.18099368633279767 1102 56-57 0.18589560316734577 1102 58-59 0.15226193609058924 1102 60-61 0.3614911200599096 1102 62-63 0.17716928374277785 1102 64-65 0.13732829911806022 1102 66-67 0.017569757660574226 1102 68-69 0.15180227870867213 1102 70-71 0.07252824555136783 1102 72-73 0.15362289245668137 1102 74-75 0.16923706594513988 1102 76-77 0.30700598852804006 1102 78-79 0.11221323393644411 1102 80-81 -0.1984078671756997 1102 82-83 0.04240130813928644 1102 84-85 0.05548782544321895 1102 86-87 0.3168805751180912 1102 88-89 0.16444516942696197 1102 90-91 0.19309366923446802 1102 92-93 0.23010592551348452 1102 94-95 0.3328230642596708 1102 96-97 0.2774451800571107 1102 98-99 0.39708978418683216 1102 100-101 0.29746556776865773 1103 1 0.12985507384445327 1103 2 0.00702739785125317 1103 3 0.08046682590481424 1103 4 0.1046423940274579 1103 5 0.060169037408350334 1103 6 0.0591675700786638 1103 7 0.06369764966047597 1103 8 0.13215214268888786 1103 9 0.10995783746503918 1103 10-11 0.0708839453838479 1103 12-13 0.06065592660373653 1103 14-15 0.03059407143317827 1103 16-17 0.08373280774877401 1103 18-19 0.14086759413901717 1103 20-21 0.22673193342296827 1103 22-23 0.10072482666020477 1103 24-25 0.12436974540288048 1103 26-27 0.20185310716757954 1103 28-29 0.2090081391800851 1103 30-31 0.23035062311642918 1103 32-33 0.1060107803571384 1103 34-35 0.1749553005786879 1103 36-37 0.22076279956028344 1103 38-39 0.05202183993755227 1103 40-41 0.15365641496961047 1103 42-43 0.30870585244935 1103 44-45 0.4550851075910103 1103 46-47 0.3810720290815155 1103 48-49 0.33322533312252745 1103 50-51 0.20368473487065586 1103 52-53 0.10330573651886965 1103 54-55 0.2611557271459475 1103 56-57 0.313192022888785 1103 58-59 0.5177762175879508 1103 60-61 0.12350047275113951 1103 62-63 0.2566593911744377 1103 64-65 0.5656547577572582 1103 66-67 0.7487148408948627 1103 68-69 0.7380525020391318 1103 70-71 0.5750606187579308 1103 72-73 0.5235299869856718 1103 74-75 0.40070063783424104 1103 76-77 0.6271744767944512 1103 78-79 0.7519365372075555 1103 80-81 0.6438703365482823 1103 82-83 0.5531638942671684 1103 84-85 0.5247187141819296 1103 86-87 0.22454022726633127 1103 88-89 0.4329815975378608 1103 90-91 0.3731912355150655 1103 92-93 0.3194691812443402 1103 94-95 0.4427599102898263 1103 96-97 0.18071784518570766 1103 98-99 0.3147565829097694 1103 100-101 -0.05980684878342757 1104 1 0.10946157659564193 1104 2 0.1311152500627557 1104 3 0.09649488484001978 1104 4 0.0224427279602466 1104 5 0.138260686585852 1104 6 0.07261068888222155 1104 7 0.10271487186879114 1104 8 0.04517945438690418 1104 9 0.07541331249694849 1104 10-11 0.17051497467625865 1104 12-13 0.14437688178022512 1104 14-15 0.24702470887297778 1104 16-17 0.15684764273091645 1104 18-19 0.10886689338069289 1104 20-21 0.3127375037365496 1104 22-23 0.16788142826838026 1104 24-25 0.08119445213909415 1104 26-27 0.1779075517987323 1104 28-29 0.07657554247722942 1104 30-31 0.05800697981671732 1104 32-33 0.22357728982989045 1104 34-35 0.26813828073220947 1104 36-37 0.06607397558290984 1104 38-39 -0.22373013452052248 1104 40-41 -0.13501489720034954 1104 42-43 -0.05777466039133117 1104 44-45 -0.07144717555825508 1104 46-47 -0.007659348087344142 1104 48-49 0.15842367275434555 1104 50-51 0.039812759125474884 1104 52-53 0.13835080314454018 1104 54-55 -0.07461655500223685 1104 56-57 -0.1854511516894135 1104 58-59 -0.3162906476269072 1104 60-61 -0.09714007755865595 1104 62-63 -0.058394041647510164 1104 64-65 -0.18141617972150925 1104 66-67 -0.09668392991783037 1104 68-69 -0.27640778891858986 1104 70-71 -0.2360331720228075 1104 72-73 -0.12180228329251719 1104 74-75 -0.2731535686820976 1104 76-77 -0.24235040191358337 1104 78-79 -0.2578919557687698 1104 80-81 -0.3554575075115345 1104 82-83 -0.2985595779188053 1104 84-85 -0.3970394741432983 1104 86-87 -0.21510875001980168 1104 88-89 -0.2822584658164118 1104 90-91 -0.20731016421392567 1104 92-93 -0.26919998574708615 1104 94-95 -0.16955985002222462 1104 96-97 -0.11212047628359656 1104 98-99 0.017738738599678783 1104 100-101 -0.04259894165535982 1105 1 0.11214060697047046 1105 2 0.22153167418696285 1105 3 0.10958366729390434 1105 4 0.043391588095801126 1105 5 0.1423081670377826 1105 6 0.056488849167898536 1105 7 0.14003407135953694 1105 8 0.06068515115607198 1105 9 0.17979337397508033 1105 10-11 0.1743531026910219 1105 12-13 0.1751908372477331 1105 14-15 0.17748736073860272 1105 16-17 0.24797722678336243 1105 18-19 0.2414567702542314 1105 20-21 0.08636624527309777 1105 22-23 0.20121782529592025 1105 24-25 0.21374177770426428 1105 26-27 0.10257301341234637 1105 28-29 0.22030364982907003 1105 30-31 0.11799751640574385 1105 32-33 0.11918531398541887 1105 34-35 0.10165203161302117 1105 36-37 0.1081282846688012 1105 38-39 -0.16227583292365466 1105 40-41 -0.15446595128135954 1105 42-43 -0.13204882017318909 1105 44-45 0.0027214569788611698 1105 46-47 -0.16173763568652788 1105 48-49 -0.2683523337543079 1105 50-51 0.031491425261101824 1105 52-53 0.10736834449590305 1105 54-55 -0.2979275233678891 1105 56-57 -0.156107769439096 1105 58-59 -0.10530424021684581 1105 60-61 -0.0645389671397183 1105 62-63 -0.16848959486558357 1105 64-65 -0.15451864667616633 1105 66-67 -0.016200683218968948 1105 68-69 -0.059246335638221126 1105 70-71 0.12602399651100882 1105 72-73 -0.06534690940268462 1105 74-75 0.10109710277755113 1105 76-77 0.1545120348126261 1105 78-79 0.02509126539931117 1105 80-81 0.10285242260219718 1105 82-83 0.11644333259068773 1105 84-85 0.09689100723114308 1105 86-87 0.15585525838153202 1105 88-89 -0.014192031309683273 1105 90-91 -0.09706142628573389 1105 92-93 0.007757970156145433 1105 94-95 -0.04226846206662671 1105 96-97 0.2283648809601715 1105 98-99 0.31814416746580676 1105 100-101 0.1436957925351301 1106 1 0.1038434129666328 1106 2 0.03580522415824561 1106 3 0.006730145441984803 1106 4 -0.030085114413161307 1106 5 0.09019822426664348 1106 6 0.04320145733835545 1106 7 0.10763739023713725 1106 8 0.027377560906757026 1106 9 0.02033477083442392 1106 10-11 0.048588236059998735 1106 12-13 -0.04250159230533512 1106 14-15 0.028925012969331476 1106 16-17 -0.04501867412555782 1106 18-19 0.020996959168861906 1106 20-21 -0.06820612249655511 1106 22-23 0.02337271945223307 1106 24-25 -0.1239629293196387 1106 26-27 -0.09889632261410952 1106 28-29 -0.05088488855783169 1106 30-31 -0.22803903877356646 1106 32-33 -0.0023566967474693 1106 34-35 -0.06370272788716846 1106 36-37 -0.05381551619854008 1106 38-39 -0.43046431190344236 1106 40-41 -0.20629866083996262 1106 42-43 -0.2599473924221485 1106 44-45 -0.12193942739509112 1106 46-47 -0.34529077535265884 1106 48-49 -0.26860976459451535 1106 50-51 -0.18985949257885437 1106 52-53 -0.03413940813286587 1106 54-55 -0.05927923330273188 1106 56-57 -0.05743254814759524 1106 58-59 -0.3157391003130883 1106 60-61 -0.2531070575969565 1106 62-63 -0.20650519913497334 1106 64-65 0.029866192627579835 1106 66-67 0.00810177821591651 1106 68-69 -0.22477931648931815 1106 70-71 -0.19826460326324025 1106 72-73 0.10457724751130826 1106 74-75 0.31260624157237515 1106 76-77 0.10257536279931401 1106 78-79 0.023665494591966763 1106 80-81 0.08554514919084255 1106 82-83 0.07992775802485141 1106 84-85 0.03483532296016989 1106 86-87 -0.19820125758908347 1106 88-89 -0.13035769795654772 1106 90-91 -0.0821254869602015 1106 92-93 -0.11364542397047117 1106 94-95 -0.2219551885364588 1106 96-97 -0.5332492104079769 1106 98-99 -0.6847321271142839 1106 100-101 -0.7243312971102078 1107 1 0.2866252731678429 1107 2 0.17724389785498573 1107 3 0.22924990021980562 1107 4 0.15021269491690248 1107 5 -0.04470165256871894 1107 6 -0.026476515565427405 1107 7 0.09974239847117872 1107 8 0.12756299980187435 1107 9 0.24278533199466068 1107 10-11 0.2839953021122241 1107 12-13 0.2439565247979445 1107 14-15 0.390800713554718 1107 16-17 0.2855953500368429 1107 18-19 0.33481769234442993 1107 20-21 0.2380759660435885 1107 22-23 0.1791379316690609 1107 24-25 0.28207905736751115 1107 26-27 0.2950437003581712 1107 28-29 0.374818464564882 1107 30-31 0.6465959648936845 1107 32-33 0.7904222573218078 1107 34-35 0.8373919151429661 1107 36-37 0.7926992012839165 1107 38-39 0.5943173822330934 1107 40-41 0.7307420665233408 1107 42-43 0.6899183719597417 1107 44-45 0.6473828903143257 1107 46-47 0.6822863409341338 1107 48-49 0.8167301836486516 1107 50-51 0.879991464368878 1107 52-53 0.9119414653673701 1107 54-55 1.1140231012899164 1107 56-57 1.0351381331327687 1107 58-59 0.9838934815775602 1107 60-61 0.9413200884430992 1107 62-63 1.016879475862602 1107 64-65 1.0575574783833872 1107 66-67 1.0711198654275478 1107 68-69 1.129837973611842 1107 70-71 1.2024494748701926 1107 72-73 1.0552525772189014 1107 74-75 1.1399577866658532 1107 76-77 1.1177702812200394 1107 78-79 1.2255274948410602 1107 80-81 1.1297425724205183 1107 82-83 1.074537575215313 1107 84-85 1.0979433618746555 1107 86-87 1.0325075618294122 1107 88-89 1.122295877277672 1107 90-91 0.775415233483745 1107 92-93 0.8775877446820495 1107 94-95 0.7462295860999291 1107 96-97 0.9088045945490535 1107 98-99 1.065264413204833 1107 100-101 1.0981974528378942 1108 1 0.06546153944129429 1108 2 -0.09218238413411228 1108 3 -0.12963267223060626 1108 4 -0.021935045070030412 1108 5 -0.024992424316778283 1108 6 -0.007986381497403272 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-0.5457651424271859 2316 84-85 -0.5757481219740193 2316 86-87 -0.5373768414890883 2316 88-89 -0.681786188896293 2316 90-91 -0.7602675385956452 2316 92-93 -0.5980895650339519 2316 94-95 -0.45366023289508917 2316 96-97 -0.44543667383973684 2316 98-99 -0.5358511694706003 2316 100-101 -0.3381251627471151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 16.0 6 25.0 7 60.0 8 175.0 9 294.0 10 526.0 11 993.0 12 1496.0 13 2065.0 14 2525.0 15 3313.0 16 4458.0 17 6799.0 18 6810.0 19 3916.0 20 2739.0 21 2642.0 22 2968.0 23 3132.0 24 3610.0 25 3907.0 26 3813.0 27 4000.0 28 4902.0 29 6160.0 30 7764.0 31 9815.0 32 12320.0 33 15983.0 34 22805.0 35 38325.0 36 56492.0 37 116573.0 38 190734.0 39 58900.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72110607114451 21.114557368538406 15.78154795382163 21.382788606495456 2 15.715923212957772 22.835311125936006 40.34963092110846 21.099134739997773 3 18.00226259399747 28.195082185399613 32.307513143009245 21.495142077593666 4 12.695758374431776 20.600303496196315 38.220886661475284 28.483051467896626 5 12.369674475245844 38.29935899888702 35.18003097700681 14.150935548860321 6 26.745035277520373 38.164770392502966 20.041890495781836 15.048303834194824 7 23.46400129098589 35.236761155964736 24.058420438835384 17.24081711421399 8 23.579790747158082 34.45701233257527 22.76543817824589 19.19775874202076 9 25.430924768462678 16.998757259716083 22.568629375585395 35.001688596235844 10-11 22.795984963994606 27.79706217283421 29.399150377522187 20.007802485648998 12-13 24.482233139408176 26.6512890727028 29.135605091408788 19.730872696480233 14-15 22.072069074514634 27.701342561014158 28.521352875609306 21.705235488861902 16-17 19.730617548290283 30.416825755704124 29.24019075711409 20.612365938891507 18-19 19.438254306131938 28.288918224460375 32.60507361204345 19.66775385736424 20-21 21.12059571678831 27.192138960656543 32.49948843020441 19.18777689235074 22-23 20.534411594916577 27.543416886960543 32.188387448822226 19.733784069300654 24-25 20.09878703890093 27.75636301325423 32.72798960556721 19.416860342277626 26-27 19.818679248265024 27.829050415953034 32.84552466812347 19.506745667658475 28-29 19.67941743114687 27.69206837231434 32.91232851186733 19.716185684671466 30-31 20.08524506709056 27.59443045597959 32.8239258481044 19.496398628825443 32-33 19.74705929815776 27.638812522927118 32.69305153392298 19.921076644992137 34-35 20.058900360223955 27.721021937888406 32.37281931399383 19.847258387893813 36-37 19.912189416645244 27.94008649625291 32.280205268468634 19.86751881863321 38-39 19.7994979129616 27.91482487676575 32.299185980159415 19.98649123011324 40-41 20.079987888689065 27.6458140281588 32.3752144018127 19.89898368133943 42-43 20.26972621449997 27.56013648515782 32.04132153035065 20.128815769991565 44-45 20.22572289387131 28.072787647793763 31.600206957016493 20.101282501318433 46-47 20.515030170140626 27.806022715362566 31.104192210497246 20.574754903999562 48-49 20.437088560633914 28.089091335588122 30.878269679632535 20.59555042414543 50-51 20.48350416159949 28.3247461698811 30.67439040012244 20.517359268396966 52-53 20.660765175322872 28.53236864301745 30.4491333259024 20.357732855757284 54-55 20.623998695704977 28.631022590589446 30.118318191421935 20.626660522283647 56-57 20.73113721549649 28.825335930832434 29.735431074496212 20.70809577917487 58-59 20.207573254543004 29.025485410646347 30.377198717660782 20.38974261714987 60-61 20.60203901682655 29.146827179348968 29.89581981586294 20.355313987961548 62-63 20.44541721572229 29.29952148970438 29.828029123241357 20.427032171331977 64-65 20.51475505837806 29.059346312150424 29.749601551868132 20.67629707760339 66-67 20.36784860143496 29.326317978579596 29.651408963294166 20.654424456691274 68-69 20.505813789053207 29.22300360523133 29.591678853246954 20.679503752468513 70-71 20.397594039499875 28.859880500523634 29.72838532633605 21.014140133640442 72-73 20.315931506302327 28.868825784296764 29.68498356204496 21.13025914735595 74-75 20.347105760528137 29.030656045360164 29.33661755310653 21.285620641005167 76-77 20.34128398241175 29.117775748108 29.396023510896164 21.14491675858409 78-79 20.383354530988367 28.852406925103374 29.355244693585053 21.408993850323206 80-81 20.234492374089708 29.066140026587117 29.23118645421376 21.46818114510942 82-83 20.5085586125198 28.883403878662033 29.208095409229458 21.399942099588706 84-85 20.52299238287115 28.7891202411661 29.14406496977521 21.543822406187534 86-87 20.190873571422035 28.90446089820215 29.10950899092215 21.795156539453668 88-89 20.450000665459967 28.571606819633732 29.125685423764907 21.852707091141397 90-91 20.371457899053556 28.617713457696087 29.120715498984346 21.89011314426601 92-93 20.39924071396843 28.658805405504324 29.032376129404696 21.90957775112254 94-95 20.29559584156143 28.42797513188519 29.078874912450857 22.197554114102523 96-97 20.202781444724213 28.391648179433883 29.165824447400183 22.239745928441724 98-99 20.15744717309623 28.39780365851731 28.95495769771773 22.489791470668727 100-101 20.233827162547726 28.492143374065233 28.88135621417936 22.392673249207682 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5077.0 1 2944.5 2 839.0 3 786.5 4 557.5 5 481.5 6 725.5 7 1231.0 8 1790.0 9 1900.5 10 1393.0 11 927.0 12 858.5 13 900.5 14 936.0 15 941.5 16 1005.5 17 1091.5 18 1132.5 19 1217.0 20 1371.0 21 1525.0 22 1695.0 23 1968.0 24 2302.5 25 2681.0 26 3249.5 27 4041.5 28 4985.0 29 6575.0 30 8542.0 31 10399.5 32 12612.0 33 14941.0 34 17413.5 35 19674.5 36 22443.5 37 24591.5 38 24494.0 39 24231.5 40 24084.5 41 23438.0 42 22799.0 43 22458.0 44 22229.5 45 22241.5 46 22198.5 47 21667.0 48 21003.0 49 20325.0 50 19197.0 51 17466.0 52 15952.0 53 14659.0 54 13040.0 55 11194.0 56 9468.0 57 8251.0 58 7283.0 59 6380.0 60 5370.0 61 4158.5 62 3081.5 63 2326.0 64 1743.5 65 1259.5 66 863.5 67 574.0 68 419.0 69 323.0 70 272.0 71 220.0 72 211.0 73 215.0 74 178.0 75 156.0 76 133.0 77 101.5 78 75.0 79 42.0 80 29.5 81 24.0 82 20.5 83 9.5 84 4.5 85 2.5 86 4.5 87 3.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019464606856532538 2 0.0 3 0.0018300057728363925 4 0.015804595310859752 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.318208058347238E-5 12-13 0.0 14-15 1.6636416116694476E-4 16-17 0.0020795520145868093 18-19 8.318208058347238E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.495462417504171E-4 28-29 0.004824560673841398 30-31 8.318208058347238E-5 32-33 2.495462417504171E-4 34-35 0.012643676248687803 36-37 0.004075921948590146 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.327283223338895E-4 60-61 0.002828190739838061 62-63 0.00981548550884974 64-65 0.001497277450502503 66-67 0.0047413785932579255 68-69 0.0033272832233388954 70-71 8.318208058347238E-5 72-73 0.007153658930178625 74-75 0.005822745640843067 76-77 0.001330913289335558 78-79 4.1591040291736193E-4 80-81 0.007985479736013348 82-83 0.00981548550884974 84-85 0.001913187853419865 86-87 0.0020795520145868093 88-89 4.990924835008342E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 8.318208058347238E-5 98-99 8.318208058347238E-5 100-101 9.981849670016685E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 601091.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.3630598025938 #Duplication Level Percentage of deduplicated Percentage of total 1 64.31870147017187 32.392866085674434 2 18.277278497032583 18.409993399494272 3 7.756359610586706 11.719020087552044 4 3.7140111149312696 7.481958555551264 5 1.9716981599476429 4.965037617105365 6 1.1954478436643639 3.6123846744810097 7 0.7137858094934368 2.5163906186832548 8 0.5059200892945136 2.0383746969978547 9 0.34745963742949815 1.5749217448964434 >10 1.1538895386693804 9.047842849198972 >50 0.03151519900695347 1.1008496875666836 >100 0.009952164122709783 0.9182376723589231 >500 0.001658694020450724 0.6014657242523848 >1k 0.002322171628631014 3.620656586187064 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4972 0.8271626093220494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4334 0.7210222744975386 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3659 0.608726465709851 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3172 0.5277071192215488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2301 0.3828039348451399 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2180 0.36267387134393964 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 0.17518146170879284 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 975 0.16220505713777114 RNA PCR Primer, Index 27 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.12477312087520857 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 727 0.12094674516836885 RNA PCR Primer, Index 27 (96% over 25bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.6636416116694476E-4 0.0 8 3.327283223338895E-4 0.0 0.0 1.6636416116694476E-4 0.0 9 3.327283223338895E-4 0.0 0.0 1.6636416116694476E-4 0.0 10-11 6.65456644667779E-4 0.0 0.0 1.6636416116694476E-4 0.0 12-13 6.65456644667779E-4 0.0 0.0 1.6636416116694476E-4 0.0 14-15 9.981849670016685E-4 0.0 0.0 1.6636416116694476E-4 0.0 16-17 0.001330913289335558 0.0 0.0 1.6636416116694476E-4 0.0 18-19 0.001330913289335558 0.0 0.0 1.6636416116694476E-4 0.0 20-21 0.0017468236922529201 0.0 0.0 1.6636416116694476E-4 0.0 22-23 0.0022459161757537546 0.0 0.0 9.981849670016687E-4 0.0 24-25 0.002329098256337227 0.0 0.0 0.0018300057728363925 0.0 26-27 0.002329098256337227 0.0 0.0 0.0041591040291736195 0.0 28-29 0.002661826578671116 0.0 0.0 0.00798547973601335 0.0 30-31 0.002661826578671116 0.0 0.0 0.013808225376856416 0.0 32-33 0.0029113728204215334 0.0 0.0 0.028947364043048388 0.0 34-35 0.0037431936262562575 0.0 0.0 0.05539926566859261 0.0 36-37 0.003992739868006675 0.0 0.0 0.08725800253206253 0.0 38-39 0.0041591040291736195 0.0 0.0 0.11529036368869272 0.0 40-41 0.0041591040291736195 0.0 0.0 0.13849816417148153 0.0 42-43 0.0041591040291736195 0.0 0.0 0.16877644150386545 0.0 44-45 0.0041591040291736195 0.0 0.0 0.2129461262936893 0.0 46-47 0.0041591040291736195 0.0 0.0 0.2615244613544372 0.0 48-49 0.004242286109757092 0.0 0.0 0.316258270378362 0.0 50-51 0.004325468190340564 0.0 0.0 0.3712416256440373 0.0 52-53 0.004325468190340564 0.0 0.0 0.4283045329242993 0.0 54-55 0.004325468190340564 0.0 0.0 0.5018374921600889 0.0 56-57 0.004325468190340564 0.0 0.0 0.5790304629415512 0.0 58-59 0.004325468190340564 0.0 0.0 0.6557243412395128 0.0 60-61 0.004325468190340564 0.0 0.0 0.7324182195374744 0.0 62-63 0.004325468190340564 0.0 0.0 0.8091952799160194 0.0 64-65 0.004325468190340564 0.0 0.0 0.8995310194296704 1.6636416116694476E-4 66-67 0.004325468190340564 0.0 0.0 1.0334541691690609 1.6636416116694476E-4 68-69 0.004325468190340564 0.0 0.0 1.1929973997281609 1.6636416116694476E-4 70-71 0.004325468190340564 0.0 0.0 1.3541210898183471 1.6636416116694476E-4 72-73 0.004325468190340564 0.0 0.0 1.5218993463552106 1.6636416116694476E-4 74-75 0.004325468190340564 0.0 0.0 1.7060644727670184 1.6636416116694476E-4 76-77 0.004325468190340564 0.0 0.0 1.9044537349586004 1.6636416116694476E-4 78-79 0.004325468190340564 0.0 0.0 2.12555170514947 1.6636416116694476E-4 80-81 0.004325468190340564 0.0 0.0 2.358128802460859 1.6636416116694476E-4 82-83 0.004325468190340564 0.0 0.0 2.6194037175735456 1.6636416116694476E-4 84-85 0.004325468190340564 0.0 0.0 2.9015573349126837 1.6636416116694476E-4 86-87 0.004325468190340564 0.0 0.0 3.2348679318106575 1.6636416116694476E-4 88-89 0.004325468190340564 0.0 0.0 3.595628615301177 1.6636416116694476E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1950 0.0 58.703484 1 GTATCAA 3460 0.0 39.399185 1 TATCAAC 4395 0.0 31.125408 2 ATCAACG 4465 0.0 30.211918 3 TCAACGC 4490 0.0 30.0437 4 CGGAGAT 95 3.48877E-5 29.999159 1 CAACGCA 4585 0.0 29.421202 5 GTGGTAT 890 0.0 29.35311 1 TGGTATC 895 0.0 29.189125 2 AACGCAG 4655 0.0 28.978779 6 ACGCAGA 5220 0.0 25.660202 7 CGCAGAG 5285 0.0 25.344606 8 GCAGAGT 5670 0.0 23.28859 9 GAGTACT 3420 0.0 22.707697 12-13 CGTCAGA 200 5.3787335E-7 21.374401 2 CAGAGTA 5625 0.0 21.027826 10-11 GTACTTT 3805 0.0 20.097992 14-15 AGAGTAC 5395 0.0 19.767076 10-11 GTACATG 3410 0.0 19.361628 1 ACATGGG 3305 0.0 19.25816 3 >>END_MODULE