FastQCFastQC Report
Fri 10 Feb 2017
SRR2031738_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031738_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862001
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.2516238380233898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.23294636549145536No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12570.14582349672448175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9600.11136878031464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.10846855166061291No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9150.10614836873739125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA650.00584560829.2283766
GGTATCA34350.025.1697181
GTATCAA44600.024.6042881
CTATAAC2303.619789E-922.7194883
GTACATG30100.022.568531
GAGTACT23100.021.7985512-13
TACATGG30200.021.3927022
CAACGCA50750.021.3418715
TCAACGC51550.021.1949734
AACGCAG51850.020.7938616
TATCAAC52150.020.7689362
ACATGGG30250.020.5721453
ATCAACG53100.020.4868243
GTACTTT24450.020.3046914-15
AGAGTAC39150.019.23062310-11
ACGCAGA56000.018.9984467
CATGGGA11350.018.8343354
CGCAGAG58250.018.1841168
AGTACTT24550.017.89880212-13
GTATAAT3852.4920155E-1017.2743231
Produced by FastQC (version 0.11.5)