FastQCFastQC Report
Fri 10 Feb 2017
SRR2031738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862001
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT29180.33851468849804117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.331322121436054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.31276065805028064No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13670.15858450280220093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.1524360180556635No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10980.1273780424848695RNA PCR Primer, Index 27 (95% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.1064963961758745No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT9110.10568433215274692RNA PCR Primer, Index 27 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.026.3609051
GTATCAA52900.023.70921
GAGTACT29900.021.76418712-13
GTACATG31150.021.5045191
ACATGGG30850.021.0927543
CAACGCA59350.020.8875585
AACGCAG60400.020.6030856
TCAACGC59950.020.599284
TACATGG32050.020.4512082
TATCAAC60650.020.4398442
ATCAACG60700.020.3447593
GTACTTT31550.020.2495814-15
GTATAGT1951.0306167E-519.4905471
GTACTAA2001.2822124E-519.0032841
ACGCAGA66350.018.6838977
AGTACTT31000.018.23384912-13
CGCAGAG67900.018.1174858
AGAGTAC46150.018.06335610-11
CATGGGA12250.017.4479474
CCAATAG2555.50607E-616.7637123
Produced by FastQC (version 0.11.5)