Basic Statistics
Measure | Value |
---|---|
Filename | SRR2031738_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 862001 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT | 2918 | 0.33851468849804117 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2856 | 0.331322121436054 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2696 | 0.31276065805028064 | No Hit |
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT | 1367 | 0.15858450280220093 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1314 | 0.1524360180556635 | No Hit |
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT | 1098 | 0.1273780424848695 | RNA PCR Primer, Index 27 (95% over 23bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 918 | 0.1064963961758745 | No Hit |
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT | 911 | 0.10568433215274692 | RNA PCR Primer, Index 27 (96% over 25bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 4055 | 0.0 | 26.360905 | 1 |
GTATCAA | 5290 | 0.0 | 23.7092 | 1 |
GAGTACT | 2990 | 0.0 | 21.764187 | 12-13 |
GTACATG | 3115 | 0.0 | 21.504519 | 1 |
ACATGGG | 3085 | 0.0 | 21.092754 | 3 |
CAACGCA | 5935 | 0.0 | 20.887558 | 5 |
AACGCAG | 6040 | 0.0 | 20.603085 | 6 |
TCAACGC | 5995 | 0.0 | 20.59928 | 4 |
TACATGG | 3205 | 0.0 | 20.451208 | 2 |
TATCAAC | 6065 | 0.0 | 20.439844 | 2 |
ATCAACG | 6070 | 0.0 | 20.344759 | 3 |
GTACTTT | 3155 | 0.0 | 20.24958 | 14-15 |
GTATAGT | 195 | 1.0306167E-5 | 19.490547 | 1 |
GTACTAA | 200 | 1.2822124E-5 | 19.003284 | 1 |
ACGCAGA | 6635 | 0.0 | 18.683897 | 7 |
AGTACTT | 3100 | 0.0 | 18.233849 | 12-13 |
CGCAGAG | 6790 | 0.0 | 18.117485 | 8 |
AGAGTAC | 4615 | 0.0 | 18.063356 | 10-11 |
CATGGGA | 1225 | 0.0 | 17.447947 | 4 |
CCAATAG | 255 | 5.50607E-6 | 16.763712 | 3 |