##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031738_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 862001 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73643882083663 34.0 31.0 34.0 31.0 34.0 2 32.90193050820127 34.0 31.0 34.0 31.0 34.0 3 32.95763113963905 34.0 31.0 34.0 31.0 34.0 4 36.359482181575196 37.0 37.0 37.0 35.0 37.0 5 36.327555304460205 37.0 37.0 37.0 35.0 37.0 6 36.35688473679265 37.0 37.0 37.0 35.0 37.0 7 36.30532215159843 37.0 37.0 37.0 35.0 37.0 8 36.29439989048737 37.0 37.0 37.0 35.0 37.0 9 38.14114832813419 39.0 39.0 39.0 37.0 39.0 10-11 38.106764377303506 39.0 39.0 39.0 36.0 39.0 12-13 38.05581664058394 39.0 39.0 39.0 35.0 39.0 14-15 39.52521864823822 41.0 39.0 41.0 37.0 41.0 16-17 39.49265314077362 41.0 39.0 41.0 36.5 41.0 18-19 39.50581147817694 41.0 39.0 41.0 36.5 41.0 20-21 39.49568852008292 41.0 39.0 41.0 36.5 41.0 22-23 39.398245477673456 41.0 39.0 41.0 36.0 41.0 24-25 39.32708894769263 41.0 39.0 41.0 36.0 41.0 26-27 39.17507810315765 41.0 39.0 41.0 36.0 41.0 28-29 39.07086650711542 40.0 39.0 41.0 35.0 41.0 30-31 38.897375409077256 40.0 38.5 41.0 35.0 41.0 32-33 38.67654735899379 40.0 38.0 41.0 35.0 41.0 34-35 38.5256455618961 40.0 38.0 41.0 35.0 41.0 36-37 38.405456026153104 40.0 38.0 41.0 34.5 41.0 38-39 38.46978193760796 40.0 38.0 41.0 35.0 41.0 40-41 38.38540732551354 40.0 38.0 41.0 34.5 41.0 42-43 38.26361512341633 40.0 38.0 41.0 34.0 41.0 44-45 38.06092568338087 40.0 38.0 41.0 33.5 41.0 46-47 37.814819820394646 40.0 38.0 41.0 33.0 41.0 48-49 37.533056226152866 40.0 37.0 41.0 33.0 41.0 50-51 37.345378949676395 40.0 37.0 41.0 32.5 41.0 52-53 37.41365091223793 40.0 37.0 41.0 33.0 41.0 54-55 37.31276065805028 40.0 37.0 41.0 33.0 41.0 56-57 37.101007423425266 40.0 36.0 41.0 32.5 41.0 58-59 36.88276057684388 39.5 36.0 41.0 32.0 41.0 60-61 36.569251659800855 39.0 35.0 41.0 31.5 41.0 62-63 36.26775258961416 39.0 35.0 41.0 31.0 41.0 64-65 35.913424694402906 38.5 35.0 40.5 31.0 41.0 66-67 35.520977353854576 37.5 35.0 40.0 30.5 41.0 68-69 35.09153005622963 37.0 35.0 39.5 30.0 41.0 70-71 34.72101192458014 36.5 35.0 39.0 29.0 41.0 72-73 34.32929776183555 36.0 34.5 39.0 29.0 41.0 74-75 33.95492290612192 35.5 34.0 38.0 29.0 40.0 76-77 33.04940713525855 35.0 33.5 37.0 27.5 39.0 78-79 33.259716636059586 35.0 34.0 37.0 29.0 39.0 80-81 33.05659448190895 35.0 34.0 36.0 29.0 38.5 82-83 32.8071574162907 35.0 34.0 36.0 28.5 37.0 84-85 32.54111364139949 35.0 34.0 35.5 27.5 37.0 86-87 32.32098454642164 35.0 34.0 35.0 27.0 36.5 88-89 32.10903757652254 35.0 34.0 35.0 27.0 36.0 90-91 31.923550552725576 35.0 33.5 35.0 26.0 36.0 92-93 31.760162111180847 35.0 33.0 35.0 26.0 36.0 94-95 31.590869964187974 35.0 33.0 35.0 25.0 35.0 96-97 31.42361783803035 35.0 33.0 35.0 25.0 35.0 98-99 31.21759313504277 35.0 33.0 35.0 24.0 35.0 100-101 30.480322528628157 34.5 32.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2468632055800697 1101 2 0.1882147798137055 1101 3 0.17268071323030654 1101 4 0.1970643837000594 1101 5 0.14694805480770157 1101 6 0.11889636484217192 1101 7 0.1108510733113448 1101 8 0.07147919720576823 1101 9 0.15144860462836363 1101 10-11 0.0857641347831617 1101 12-13 0.1534334424382493 1101 14-15 0.18051925416937564 1101 16-17 0.13656744754844397 1101 18-19 0.17749671717603377 1101 20-21 0.1496523916701591 1101 22-23 0.006567891583678431 1101 24-25 0.04461221316566366 1101 26-27 0.11039550462640335 1101 28-29 -0.0012900318182929027 1101 30-31 -0.06142555935932137 1101 32-33 -0.0031984532016622325 1101 34-35 -0.2782989636364803 1101 36-37 -0.12308420292181665 1101 38-39 -0.05655562751488219 1101 40-41 -0.43781862105434044 1101 42-43 -0.4205004756315276 1101 44-45 -0.2915773685750409 1101 46-47 -0.29666819834778124 1101 48-49 -0.32498413296269035 1101 50-51 -0.19539298140372807 1101 52-53 -0.24158482049468688 1101 54-55 -0.1948439369742445 1101 56-57 -0.4609715721786003 1101 58-59 -0.47126973215623025 1101 60-61 -0.415045865912802 1101 62-63 -0.39936837998679664 1101 64-65 -0.43349139280204696 1101 66-67 -0.8528756222171126 1101 68-69 -0.6766372186892085 1101 70-71 -0.5775902973826277 1101 72-73 -0.7035003882112605 1101 74-75 -0.675363845471594 1101 76-77 -0.7025142767547052 1101 78-79 -0.5934016764263532 1101 80-81 -0.48924019320514134 1101 82-83 -0.4838978122152966 1101 84-85 -0.6253633545455486 1101 86-87 -0.6389869941196338 1101 88-89 -0.5291278755468802 1101 90-91 -0.5991044441649329 1101 92-93 -0.6841359266978344 1101 94-95 -0.6835565744959453 1101 96-97 -0.648451518908697 1101 98-99 -0.9569297844823872 1101 100-101 -0.8894040536046681 1102 1 0.162865116992279 1102 2 0.1312134618641565 1102 3 0.1378841476064352 1102 4 0.016414734767906225 1102 5 0.1491966617119047 1102 6 0.16450628260520261 1102 7 0.1403854748774691 1102 8 0.07136551442312111 1102 9 0.17668618237582479 1102 10-11 0.29967466836004775 1102 12-13 0.19948460354410003 1102 14-15 0.16833303791774767 1102 16-17 0.2236580931895702 1102 18-19 0.3466133441500645 1102 20-21 0.2092876818888172 1102 22-23 0.19942013479546716 1102 24-25 0.16907751872185628 1102 26-27 0.25095944830139416 1102 28-29 0.25175146432420803 1102 30-31 0.1609870975807297 1102 32-33 0.20615224469500504 1102 34-35 0.2936786158065985 1102 36-37 0.29819528716272714 1102 38-39 0.2725984184360897 1102 40-41 0.2574036191515958 1102 42-43 0.3715399954391003 1102 44-45 0.4011688991614051 1102 46-47 0.44651565176049957 1102 48-49 0.5193465644715545 1102 50-51 0.5523458873177063 1102 52-53 0.6765866979219268 1102 54-55 0.8740295244545351 1102 56-57 0.7220355493733379 1102 58-59 0.48493734777625264 1102 60-61 0.6430681945279062 1102 62-63 0.6319348486042315 1102 64-65 0.46675581907954466 1102 66-67 0.42676764506460074 1102 68-69 0.7144537121222925 1102 70-71 0.4855893907797082 1102 72-73 0.6578702022455474 1102 74-75 0.5070218744294479 1102 76-77 0.4973940062884523 1102 78-79 0.36493358373380147 1102 80-81 0.2751252641850144 1102 82-83 0.44347719452859735 1102 84-85 0.5799130680306916 1102 86-87 0.6215045779398309 1102 88-89 0.4762691663885832 1102 90-91 0.48524143666468333 1102 92-93 0.574237099268089 1102 94-95 0.7300348765588005 1102 96-97 0.39101952859143907 1102 98-99 0.37886025305219917 1102 100-101 0.43044052854366655 1103 1 0.2617547111323759 1103 2 0.18856315216160624 1103 3 0.1859828876386871 1103 4 0.1503109315183977 1103 5 0.1940159836493507 1103 6 0.10795182383686353 1103 7 0.221593934973626 1103 8 0.10913354027908895 1103 9 0.21808564395301033 1103 10-11 0.2460822646450751 1103 12-13 0.08658361351731969 1103 14-15 0.21683942530493994 1103 16-17 0.2608789413879009 1103 18-19 0.2728838906711104 1103 20-21 0.3458340424278177 1103 22-23 0.16815805128886296 1103 24-25 0.12293949862949916 1103 26-27 0.19486826661653112 1103 28-29 0.243938679335848 1103 30-31 0.3396159798062044 1103 32-33 0.28232940622915237 1103 34-35 0.21789907003700648 1103 36-37 0.17430149799393035 1103 38-39 0.26939378128783176 1103 40-41 0.3263078776529156 1103 42-43 0.4490905728939154 1103 44-45 0.443380007597419 1103 46-47 0.27379811216307814 1103 48-49 0.35795560238731383 1103 50-51 0.37256975593670205 1103 52-53 0.3364938965911932 1103 54-55 0.42633879656233375 1103 56-57 0.39280148865851316 1103 58-59 0.4553131493915288 1103 60-61 0.4631151423709312 1103 62-63 0.4308434115697821 1103 64-65 0.39641157137586447 1103 66-67 0.3742942196595038 1103 68-69 0.20367522422951367 1103 70-71 0.2511611549534365 1103 72-73 0.2347030650060944 1103 74-75 0.34995999957991586 1103 76-77 0.42457195575841666 1103 78-79 0.44189503324389534 1103 80-81 0.35563242491662805 1103 82-83 0.46795336311767954 1103 84-85 0.38842277496233635 1103 86-87 0.45947432995892257 1103 88-89 0.2822162579569394 1103 90-91 0.15625907767236313 1103 92-93 0.15699498409813373 1103 94-95 0.243241921288746 1103 96-97 0.15364474107876447 1103 98-99 0.4048530157157124 1103 100-101 0.27365680233677736 1104 1 0.06638022217646267 1104 2 0.12543753389337553 1104 3 0.16295563887176456 1104 4 0.07885987760887048 1104 5 0.11702559231583365 1104 6 0.15281993814471662 1104 7 0.17246452581322558 1104 8 0.0586811235853304 1104 9 -0.004788378059068066 1104 10-11 0.17988432888271433 1104 12-13 0.11993464459848724 1104 14-15 0.23269995091075657 1104 16-17 0.11760327850059582 1104 18-19 0.05733915745168616 1104 20-21 0.15106830987941322 1104 22-23 0.15449389113059198 1104 24-25 0.1330522915070489 1104 26-27 0.22394849102627035 1104 28-29 0.21296214412827652 1104 30-31 0.3105772615151494 1104 32-33 0.3570412845885471 1104 34-35 0.2590584396974194 1104 36-37 0.364115896466501 1104 38-39 0.43382891760084163 1104 40-41 0.4187013625758098 1104 42-43 0.23441833287787261 1104 44-45 0.2868855106169903 1104 46-47 0.45694559032047977 1104 48-49 0.3577515306370671 1104 50-51 0.5206030576792671 1104 52-53 0.6050246491663032 1104 54-55 0.5631628521251955 1104 56-57 0.4256051577994029 1104 58-59 0.49434446575807556 1104 60-61 0.6403713458062512 1104 62-63 0.6249095930760404 1104 64-65 0.6430348756779765 1104 66-67 0.4888978016375489 1104 68-69 0.3183531713368808 1104 70-71 0.3096925647087687 1104 72-73 0.4095017603304356 1104 74-75 0.47097541218533223 1104 76-77 0.5649992612503709 1104 78-79 0.6202494194029953 1104 80-81 0.5947301510730867 1104 82-83 0.477598347449117 1104 84-85 0.5308389153083652 1104 86-87 0.5111779831891354 1104 88-89 0.36743610203228627 1104 90-91 0.508419471150372 1104 92-93 0.3115066992001374 1104 94-95 0.45818049223307966 1104 96-97 0.5059045762994749 1104 98-99 0.3089686932685112 1104 100-101 0.36676021483150123 1105 1 0.16521758655786556 1105 2 0.12121810350814144 1105 3 0.11957602683929736 1105 4 0.1235364935290093 1105 5 0.13592050499312336 1105 6 0.10945533001326879 1105 7 0.09323059165701153 1105 8 0.1064152011121493 1105 9 0.09964544140066067 1105 10-11 0.0580718242981888 1105 12-13 0.11145160567503609 1105 14-15 0.10779914336745833 1105 16-17 0.23043594832808623 1105 18-19 0.11264020239003258 1105 20-21 0.03658687804047389 1105 22-23 0.10689318161278294 1105 24-25 0.11119410837942922 1105 26-27 0.13614732939091567 1105 28-29 0.07824628467148642 1105 30-31 0.12106632200421075 1105 32-33 -0.04121309488344593 1105 34-35 0.0426263340166102 1105 36-37 0.2636097648722924 1105 38-39 0.21214628011509262 1105 40-41 0.14868365797261873 1105 42-43 0.1421768500923477 1105 44-45 0.23554132779612047 1105 46-47 0.34564165129634006 1105 48-49 0.23600320940688135 1105 50-51 0.17492484801142183 1105 52-53 0.19408780558696748 1105 54-55 0.17826027745578443 1105 56-57 0.1948318233991202 1105 58-59 0.1841588174895321 1105 60-61 0.023580146761169374 1105 62-63 -0.15270341964060208 1105 64-65 -0.12722955974189887 1105 66-67 0.11727284581569819 1105 68-69 -0.06685364910309488 1105 70-71 -0.10339567156600538 1105 72-73 -0.16841099698647355 1105 74-75 0.057383711676529 1105 76-77 0.08116513104318557 1105 78-79 0.08065662527651796 1105 80-81 -0.07331187318654742 1105 82-83 0.0584343648136354 1105 84-85 0.25076776347555096 1105 86-87 0.026097695647706587 1105 88-89 -0.10829842227736464 1105 90-91 -0.18005119789024704 1105 92-93 -0.10110891419268242 1105 94-95 -0.06747867915147765 1105 96-97 -0.12076919221367532 1105 98-99 -0.022299103025190448 1105 100-101 0.21680622679461337 1106 1 -0.0678130479975465 1106 2 -0.064950639204433 1106 3 0.05607142585058256 1106 4 0.0028004007471693626 1106 5 0.07260887243117509 1106 6 0.07774636564865034 1106 7 0.047058807238720135 1106 8 0.05393923631090303 1106 9 0.12410010117933723 1106 10-11 -0.010642924213257743 1106 12-13 -0.10404752024340524 1106 14-15 0.018413060969940886 1106 16-17 0.11126649751949458 1106 18-19 0.09216347225638089 1106 20-21 -0.034898929785875055 1106 22-23 -0.018895249766501365 1106 24-25 0.03146217096007575 1106 26-27 -0.015142370183404807 1106 28-29 -0.005618120666873949 1106 30-31 -0.05441638524469994 1106 32-33 0.01862837664798178 1106 34-35 -0.1208694362135958 1106 36-37 -0.029505187073667116 1106 38-39 -0.13064659444398075 1106 40-41 -0.07800927202399777 1106 42-43 -0.24654087471179054 1106 44-45 0.020451850953158157 1106 46-47 -0.004235453981017656 1106 48-49 -0.15405957074484178 1106 50-51 -0.12333075011711259 1106 52-53 0.04226253686208281 1106 54-55 -0.09212037729590605 1106 56-57 -0.017765417152368457 1106 58-59 -0.09719650770315269 1106 60-61 -0.012411735482302788 1106 62-63 8.741005188781514E-4 1106 64-65 -0.011966901912536798 1106 66-67 0.08895145853624342 1106 68-69 0.09482445666649397 1106 70-71 0.11984672130790841 1106 72-73 0.12155431775648395 1106 74-75 0.10301829161860354 1106 76-77 -0.023714026567866142 1106 78-79 -0.0662700254620745 1106 80-81 0.0849385900194406 1106 82-83 0.08551290459865868 1106 84-85 0.10917963332325797 1106 86-87 -0.0011754478897358922 1106 88-89 -0.007325239982712617 1106 90-91 0.00546504988775709 1106 92-93 -0.02687575927337349 1106 94-95 -0.012355122121949336 1106 96-97 0.14214783626879424 1106 98-99 0.11305353042147104 1106 100-101 -0.11550502800711371 1107 1 0.21616986230578306 1107 2 0.21687701660574987 1107 3 0.19070125872747212 1107 4 0.20743705426880865 1107 5 0.11242308776274257 1107 6 0.13848299528071095 1107 7 0.05869371924548261 1107 8 0.18064396471421418 1107 9 0.12122989077802515 1107 10-11 0.24736008102513551 1107 12-13 0.23273306619355338 1107 14-15 0.3107166385192883 1107 16-17 0.30670480184489435 1107 18-19 0.3223749468659278 1107 20-21 0.338037998292684 1107 22-23 0.40747987184524703 1107 24-25 0.31476474791737274 1107 26-27 0.2598932044991855 1107 28-29 0.3740303910822931 1107 30-31 0.4563538282726398 1107 32-33 0.4345232487003088 1107 34-35 0.46433755477415417 1107 36-37 0.4139916570797979 1107 38-39 0.34470127574910947 1107 40-41 0.5688492498003299 1107 42-43 0.501181826263533 1107 44-45 0.47611939126247194 1107 46-47 0.43074943327060566 1107 48-49 0.6240297139183966 1107 50-51 0.6546615913721467 1107 52-53 0.9139677672925473 1107 54-55 0.7508013861004272 1107 56-57 0.8000774933586925 1107 58-59 0.5914947248254379 1107 60-61 0.7704468071155688 1107 62-63 0.5843923760383092 1107 64-65 0.6106991046172752 1107 66-67 0.7401507840887334 1107 68-69 0.5309189970346679 1107 70-71 0.6524291489727148 1107 72-73 0.6168456493055956 1107 74-75 0.5662638452160635 1107 76-77 0.5597367349553863 1107 78-79 0.6831967870809876 1107 80-81 0.7372666110976596 1107 82-83 0.7512320671481234 1107 84-85 0.8362560349311252 1107 86-87 0.8074942147826434 1107 88-89 0.8457842123229753 1107 90-91 0.8634017529196534 1107 92-93 0.7612038656950304 1107 94-95 0.9102547958572025 1107 96-97 0.9901469984027891 1107 98-99 1.0637406151671165 1107 100-101 1.3773982123184787 1108 1 0.034267883669997445 1108 2 -0.05749143667583212 1108 3 -0.11854522673974088 1108 4 -0.04664608925761371 1108 5 -0.012578937204295926 1108 6 -0.02932197285440452 1108 7 -0.037278378328771566 1108 8 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-0.9044515177133547 2316 56-57 -0.8494549670480751 2316 58-59 -0.8125155140824134 2316 60-61 -0.8107727946736887 2316 62-63 -0.6740877366733784 2316 64-65 -0.5844713578540066 2316 66-67 -0.6512107767634845 2316 68-69 -0.6325478171863494 2316 70-71 -0.5743217414681823 2316 72-73 -0.7880724673305295 2316 74-75 -0.6411065874963597 2316 76-77 -1.0528587026393552 2316 78-79 -0.8107131086700079 2316 80-81 -0.6279594632436911 2316 82-83 -0.48889752338855885 2316 84-85 -0.4830030624454409 2316 86-87 -0.5102304413630279 2316 88-89 -0.6395246753466282 2316 90-91 -0.5934037264304877 2316 92-93 -0.44334528757239866 2316 94-95 -0.3080090071391446 2316 96-97 -0.4386700404046273 2316 98-99 -0.48421067329853784 2316 100-101 -0.248973485145509 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 2.0 5 14.0 6 22.0 7 43.0 8 149.0 9 295.0 10 447.0 11 866.0 12 1266.0 13 1751.0 14 2159.0 15 2612.0 16 3317.0 17 4867.0 18 5186.0 19 3101.0 20 2385.0 21 2653.0 22 3025.0 23 3330.0 24 3826.0 25 4525.0 26 4593.0 27 4784.0 28 6176.0 29 7884.0 30 10271.0 31 13146.0 32 17195.0 33 23185.0 34 33310.0 35 54197.0 36 97208.0 37 198051.0 38 274109.0 39 71971.0 40 76.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.513126062413335 19.447574071603032 14.471099456392464 23.568200409591164 2 16.58223134311909 21.543594496990142 38.16573298638865 23.708441173502116 3 18.557618351003665 25.568712434352562 30.98152996957045 24.892139245073324 4 13.85370845889024 18.660241556600106 36.09958843248123 31.386461552028422 5 14.295343044845657 36.53580448282543 34.36886964168255 14.79998283064637 6 30.594512071331703 35.44369438086499 18.543365958972206 15.418427588831104 7 26.179668005025512 32.66156303762989 22.860762342503083 18.298006614841515 8 25.09092216830375 32.690449315024 22.004266816395806 20.214361700276452 9 25.569575905364378 16.008798133644856 21.608907646278833 36.81271831471194 10-11 24.23349856902718 25.732104719136057 28.729607042219207 21.304789669617552 12-13 25.351014673996897 24.093533534183837 28.43088349085442 22.124568300964846 14-15 22.99385150812065 25.848723897911835 26.45145011600928 24.705974477958236 16-17 22.038403064542045 28.247244597810763 27.35993577640537 22.354416561241827 18-19 21.948665893271464 26.971345707656614 29.228654292343386 21.851334106728537 20-21 22.9483492478547 25.997881672991095 28.957565014425736 22.096204064728465 22-23 22.650263746793797 26.184018348006553 28.80669511984325 22.359022785356398 24-25 22.700089675069982 26.258960256426615 28.904026793472397 22.136923275031002 26-27 22.2911319348374 26.395088152807865 29.364992357866466 21.948787554488266 28-29 22.064388885666222 26.409942572074947 29.208190730320784 22.317477811938048 30-31 22.468245396467058 26.17259144710969 28.940975706524704 22.41818744989855 32-33 22.226740139211138 26.187470997679814 29.04350348027842 22.542285382830627 34-35 22.477028827740718 26.306379369656764 28.816254406711717 22.4003373958908 36-37 22.41066073295149 26.233428889969108 29.154828433107067 22.201081943972333 38-39 22.4213196968449 26.20780022296958 29.03273894113812 22.3381411390474 40-41 22.48129642541018 26.357103994078894 28.815685828670734 22.345913751840197 42-43 22.68790871472307 26.16893715900562 28.44265841918977 22.700495707081547 44-45 22.595449425232687 26.342776864528002 28.477519167611177 22.58425454262814 46-47 22.941504708231196 26.16290468340524 28.125837441023847 22.76975316733971 48-49 22.759254339612134 26.367602821806475 28.136916314482235 22.73622652409916 50-51 22.533326527463426 26.629725487557437 28.102983639230118 22.73396434574902 52-53 22.63483453035437 26.90095487128205 27.803332014696036 22.660878583667536 54-55 22.608906486187372 26.63187165676142 27.812090705231203 22.94713115182001 56-57 22.857920118422136 26.85130295672511 27.397764039716893 22.893012885135864 58-59 22.75977918703846 26.92139701263288 27.765019927575114 22.553803872753544 60-61 22.79778229455249 27.199027346595944 27.664123660629347 22.339066698222215 62-63 22.48265011860514 27.37043497833549 27.564600498785524 22.582314404273845 64-65 22.47467745733945 27.41018052643974 27.41725726496817 22.69788475125264 66-67 22.493536506772674 27.326146969133593 27.228056172208227 22.952260351885506 68-69 22.569855928944204 27.295075643540006 27.390554549140607 22.744513878375187 70-71 22.709312402189788 27.047706441175823 27.400722272944 22.842258883690388 72-73 22.62738155180535 27.01406597690321 27.475642099547766 22.882910371743677 74-75 22.53121278408811 27.336789464311302 27.193567118144802 22.938430633455788 76-77 22.65009098180886 27.114493225234064 27.093553197820615 23.141862595136462 78-79 22.668118334518567 27.05403847113157 27.20142877527416 23.076414419075704 80-81 22.675349445898537 27.13815178853186 27.082405190176534 23.10409357539307 82-83 22.71523294060319 27.032596021649468 27.24288357900721 23.00928745874013 84-85 22.725464014041943 26.902489474923698 27.141413637529016 23.230632873505346 86-87 22.582899887875534 27.089920573186298 27.1457217816711 23.18145775726706 88-89 22.638638743151805 26.942247512318772 26.92786231978631 23.491251424743112 90-91 22.81412666574633 26.754435319680603 26.9705603589787 23.460877655594366 92-93 22.81644684866955 26.86000364268719 27.11499174594925 23.20855776269401 94-95 22.761226495096874 26.711859963039487 27.131697062996444 23.395216478867194 96-97 22.52752607015537 26.69405255910376 27.216789771705603 23.56163159903527 98-99 22.5799041996471 26.71006182127399 26.892892235623854 23.817141743455053 100-101 22.565167179140936 26.815542326546165 26.767514177874684 23.851776316438215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1274.0 1 774.5 2 291.0 3 275.0 4 206.0 5 205.5 6 297.5 7 539.5 8 845.5 9 954.5 10 786.5 11 563.5 12 493.0 13 537.5 14 596.0 15 622.0 16 667.5 17 751.5 18 861.5 19 911.0 20 975.5 21 1104.5 22 1259.0 23 1439.0 24 1691.5 25 2076.5 26 2613.0 27 3379.0 28 4400.0 29 5668.5 30 7286.5 31 9288.5 32 11862.0 33 14331.5 34 16252.0 35 18383.0 36 20540.0 37 22479.5 38 24421.0 39 25816.5 40 26758.5 41 27951.5 42 28977.5 43 30309.0 44 32105.5 45 33498.0 46 34898.0 47 37041.0 48 39687.5 49 38865.0 50 35265.0 51 32755.0 52 30845.0 53 29374.5 54 27730.0 55 25648.5 56 23300.0 57 20566.5 58 18526.0 59 16963.5 60 14572.5 61 11850.5 62 9463.5 63 7336.0 64 5494.0 65 3955.0 66 2767.0 67 2022.0 68 1558.5 69 1201.5 70 905.5 71 688.0 72 530.5 73 427.0 74 308.0 75 216.0 76 164.5 77 121.0 78 85.5 79 58.5 80 40.5 81 26.5 82 17.0 83 15.0 84 10.5 85 3.5 86 3.5 87 3.5 88 3.0 89 2.5 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019257518262739835 2 0.0 3 0.0011600914616108334 4 0.020185591432028503 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1600914616108334E-4 16-17 0.0014501143270135416 18-19 1.1600914616108334E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.0603201156379166E-4 28-29 0.005916466454215251 30-31 0.0 32-33 1.1600914616108334E-4 34-35 0.012238964919994292 36-37 0.003944310969476833 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.22041157724875E-4 60-61 0.0033642652386714172 62-63 0.01247098321231646 64-65 0.0025522012155438337 66-67 0.005162407004168209 68-69 0.003538278957913042 70-71 0.0 72-73 0.007308576208148251 74-75 0.006322498465779042 76-77 0.0017981417654967919 78-79 4.6403658464433335E-4 80-81 0.007540594500470417 82-83 0.009976786569853166 84-85 0.0013921097539330001 86-87 0.0014501143270135416 88-89 4.0603201156379166E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.540594500470417E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 862001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.07324759614397 #Duplication Level Percentage of deduplicated Percentage of total 1 64.74796694441584 35.65880814876759 2 19.995888989664916 22.024770904656503 3 7.597181403301656 12.552043573705578 4 3.3037906431733064 7.27801920389229 5 1.5752051955543283 4.3375832874747955 6 0.8418597563299345 2.7818370480952748 7 0.5067142585147433 1.9534479873775272 8 0.3418672959546523 1.5062193788107836 9 0.21331138931442412 1.0572975862911662 >10 0.8504247718040139 7.225740560234831 >50 0.01500939201210917 0.5575458776348782 >100 0.008032127053460684 0.9991409747796421 >500 0.0014796023343456252 0.6416480277661567 >1k 0.0012682305722962503 1.4258974405129994 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2918 0.33851468849804117 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2856 0.331322121436054 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2696 0.31276065805028064 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1367 0.15858450280220093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1314 0.1524360180556635 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1098 0.1273780424848695 RNA PCR Primer, Index 27 (95% over 23bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 918 0.1064963961758745 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 911 0.10568433215274692 RNA PCR Primer, Index 27 (96% over 25bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 1.1600914616108334E-4 12-13 0.0 0.0 0.0 0.0 1.1600914616108334E-4 14-15 0.0 0.0 0.0 0.0 1.1600914616108334E-4 16-17 0.0 0.0 0.0 5.800457308054167E-5 1.1600914616108334E-4 18-19 0.0 0.0 0.0 1.1600914616108334E-4 1.1600914616108334E-4 20-21 0.0 0.0 0.0 1.1600914616108334E-4 1.1600914616108334E-4 22-23 0.0 0.0 0.0 2.3201829232216668E-4 1.1600914616108334E-4 24-25 0.0 0.0 0.0 6.380503038859583E-4 1.1600914616108334E-4 26-27 0.0 0.0 0.0 9.280731692886667E-4 1.1600914616108334E-4 28-29 0.0 0.0 0.0 0.0019141509116578752 1.1600914616108334E-4 30-31 0.0 0.0 0.0 0.0048723841387655 1.1600914616108334E-4 32-33 0.0 0.0 0.0 0.009976786569853166 1.1600914616108334E-4 34-35 0.0 0.0 0.0 0.017517381070323586 3.4802743848325003E-4 36-37 0.0 0.0 0.0 0.026508089897807542 3.4802743848325003E-4 38-39 0.0 0.0 0.0 0.033120611228989294 4.6403658464433335E-4 40-41 0.0 0.0 0.0 0.0410672377410235 4.6403658464433335E-4 42-43 0.0 0.0 0.0 0.05516234899959513 4.6403658464433335E-4 44-45 0.0 0.0 0.0 0.07117161116982462 5.22041157724875E-4 46-47 0.0 0.0 0.0 0.08961706540943687 5.800457308054167E-4 48-49 0.0 0.0 0.0 0.11310891750705626 5.800457308054167E-4 50-51 0.0 0.0 0.0 0.1358467101546286 5.800457308054167E-4 52-53 0.0 0.0 0.0 0.16096269029850313 5.800457308054167E-4 54-55 0.0 0.0 0.0 0.1947213518313784 5.800457308054167E-4 56-57 0.0 0.0 0.0 0.23027815512975042 6.960548769665001E-4 58-59 0.0 0.0 0.0 0.26415282580878674 8.120640231275833E-4 60-61 0.0 0.0 0.0 0.30098572971493076 8.120640231275833E-4 62-63 0.0 0.0 0.0 0.3309740939975708 8.120640231275833E-4 64-65 0.0 0.0 0.0 0.36977915338845313 8.120640231275833E-4 66-67 0.0 0.0 0.0 0.43857257706197555 8.120640231275833E-4 68-69 0.0 0.0 0.0 0.5236072811980497 8.120640231275833E-4 70-71 0.0 0.0 0.0 0.6045816652184859 8.120640231275833E-4 72-73 0.0 0.0 0.0 0.6928646254470703 8.120640231275833E-4 74-75 0.0 0.0 0.0 0.7936185688879711 8.120640231275833E-4 76-77 0.0 0.0 0.0 0.9034792303025171 8.120640231275833E-4 78-79 0.0 0.0 0.0 1.032191377968239 8.120640231275833E-4 80-81 0.0 0.0 0.0 1.1622376308148135 8.120640231275833E-4 82-83 0.0 0.0 0.0 1.3020286519389188 8.120640231275833E-4 84-85 0.0 0.0 0.0 1.4673996897915433 8.120640231275833E-4 86-87 0.0 0.0 0.0 1.6839307611012053 8.120640231275833E-4 88-89 0.0 0.0 0.0 1.9304501966935073 8.120640231275833E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4055 0.0 26.360905 1 GTATCAA 5290 0.0 23.7092 1 GAGTACT 2990 0.0 21.764187 12-13 GTACATG 3115 0.0 21.504519 1 ACATGGG 3085 0.0 21.092754 3 CAACGCA 5935 0.0 20.887558 5 AACGCAG 6040 0.0 20.603085 6 TCAACGC 5995 0.0 20.59928 4 TACATGG 3205 0.0 20.451208 2 TATCAAC 6065 0.0 20.439844 2 ATCAACG 6070 0.0 20.344759 3 GTACTTT 3155 0.0 20.24958 14-15 GTATAGT 195 1.0306167E-5 19.490547 1 GTACTAA 200 1.2822124E-5 19.003284 1 ACGCAGA 6635 0.0 18.683897 7 AGTACTT 3100 0.0 18.233849 12-13 CGCAGAG 6790 0.0 18.117485 8 AGAGTAC 4615 0.0 18.063356 10-11 CATGGGA 1225 0.0 17.447947 4 CCAATAG 255 5.50607E-6 16.763712 3 >>END_MODULE