FastQCFastQC Report
Fri 10 Feb 2017
SRR2031736_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031736_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277621
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.21432096275137688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.20459547368534803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.10157733024519038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.046.953211
GTATCAA6800.033.5380061
GTGGTAT2400.029.6951081
ACTATAG650.00583436129.2329883
ATGCGGA504.9520717E-528.49702620-21
CCGTTCT856.3987356E-427.9382619
TATCAAC8650.026.9144122
TGGTATC2500.026.6068172
ATAAGAC908.9264044E-426.3908943
ATCAACG9100.024.534833
TCAACGC9250.024.1369694
CAACGCA9500.023.9974945
AACGCAG9600.023.747526
ACGCAGA10600.021.0591227
CGCAGAG10800.020.669148
TAGACTG1404.842387E-420.3550175
GCCTAGA1200.00474750819.796741
GTAGACT1250.0060172219.0014444
ATTATAC1250.0060172219.0014443
GAGTACT6750.018.6529312-13
Produced by FastQC (version 0.11.5)