##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031736_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 277621 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60295150582989 33.0 31.0 34.0 30.0 34.0 2 31.914437308416872 34.0 31.0 34.0 30.0 34.0 3 32.012214493860334 34.0 31.0 34.0 30.0 34.0 4 35.39691161691659 37.0 35.0 37.0 35.0 37.0 5 35.3956184870741 37.0 37.0 37.0 35.0 37.0 6 35.4146300171817 37.0 37.0 37.0 35.0 37.0 7 35.386465721253074 37.0 37.0 37.0 35.0 37.0 8 35.38157776248915 37.0 37.0 37.0 35.0 37.0 9 37.125411982522934 39.0 38.0 39.0 35.0 39.0 10-11 37.052589681616304 39.0 38.0 39.0 35.0 39.0 12-13 36.99577841733875 39.0 38.0 39.0 35.0 39.0 14-15 38.37148126402542 41.0 39.0 41.0 34.5 41.0 16-17 38.30295979050577 41.0 39.0 41.0 34.0 41.0 18-19 38.31247095860904 41.0 39.0 41.0 34.0 41.0 20-21 38.30942363870169 41.0 39.0 41.0 34.0 41.0 22-23 38.20579855270314 41.0 39.0 41.0 34.0 41.0 24-25 38.135917311730736 41.0 39.0 41.0 34.0 41.0 26-27 38.01991023733795 40.5 38.0 41.0 34.0 41.0 28-29 37.89672431120124 40.0 38.0 41.0 34.0 41.0 30-31 37.74173603581862 40.0 38.0 41.0 33.0 41.0 32-33 37.52817690304408 40.0 38.0 41.0 33.0 41.0 34-35 37.36635016803484 40.0 38.0 41.0 33.0 41.0 36-37 37.201870535730365 40.0 38.0 41.0 32.0 41.0 38-39 37.01003526390295 40.0 37.5 41.0 31.0 41.0 40-41 36.90221380947407 40.0 37.0 41.0 31.0 41.0 42-43 36.87992082731493 40.0 37.0 41.0 31.0 41.0 44-45 36.7473822225264 40.0 37.0 41.0 30.5 41.0 46-47 36.67538658819038 40.0 37.0 41.0 30.5 41.0 48-49 36.70946542228434 40.0 37.0 41.0 30.5 41.0 50-51 36.161850148223664 39.5 36.5 40.5 29.5 41.0 52-53 36.1842818086528 39.5 37.0 40.5 30.0 41.0 54-55 36.40739173189348 40.0 37.0 41.0 30.0 41.0 56-57 36.274716249851416 40.0 36.0 41.0 30.0 41.0 58-59 36.083612190720444 40.0 36.0 41.0 29.0 41.0 60-61 35.854301367691924 39.0 35.0 41.0 28.5 41.0 62-63 35.57704388356788 39.0 35.0 41.0 28.0 41.0 64-65 35.31536519211443 39.0 35.0 41.0 28.0 41.0 66-67 35.04350535442204 38.0 35.0 41.0 28.0 41.0 68-69 34.72248677153385 37.5 35.0 40.0 27.5 41.0 70-71 34.365080451406776 37.0 35.0 40.0 26.0 41.0 72-73 33.99925077713861 36.5 34.5 39.0 26.0 41.0 74-75 33.59143220433613 36.0 34.0 39.0 26.0 41.0 76-77 33.21517644558589 35.5 34.0 38.5 25.5 40.0 78-79 32.83974195035678 35.0 34.0 37.0 25.0 39.0 80-81 32.49757042874998 35.0 34.0 37.0 24.5 39.0 82-83 32.181987313639816 35.0 34.0 36.0 24.0 38.0 84-85 31.866701366251114 35.0 34.0 36.0 23.0 37.0 86-87 31.58978787627737 35.0 33.0 36.0 21.5 37.0 88-89 31.331392437891946 35.0 33.0 35.0 20.0 36.5 90-91 31.101804258323398 35.0 33.0 35.0 19.5 36.0 92-93 30.904090468660513 35.0 33.0 35.0 18.0 36.0 94-95 30.664744381729047 35.0 33.0 35.0 11.0 36.0 96-97 30.42626998678054 35.0 32.0 35.0 2.0 35.0 98-99 30.195799309130074 35.0 32.0 35.0 2.0 35.0 100-101 29.6440308910349 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26570915752308366 1101 2 0.5174966776037415 1101 3 0.5900177088499845 1101 4 0.6466223598866989 1101 5 0.41550979890259043 1101 6 0.752386829907131 1101 7 0.5507473149401321 1101 8 0.4028881564274087 1101 9 0.6325218556792933 1101 10-11 0.6014600848951375 1101 12-13 0.5096611313346671 1101 14-15 0.5798110191432215 1101 16-17 0.7473004921020774 1101 18-19 0.7790238216048948 1101 20-21 0.7861241318879735 1101 22-23 0.9734749038509918 1101 24-25 0.6759016290231159 1101 26-27 0.674155876139821 1101 28-29 0.7982898050669291 1101 30-31 0.8796134084898029 1101 32-33 0.7524890030609157 1101 34-35 0.8011078841716426 1101 36-37 0.5970818386028114 1101 38-39 0.9501001696386808 1101 40-41 0.7300148979479175 1101 42-43 0.8366257341147261 1101 44-45 0.4677254287115886 1101 46-47 0.5441249251339926 1101 48-49 0.37933412002336553 1101 50-51 0.7133541756506645 1101 52-53 0.661501700719981 1101 54-55 1.0527883415963188 1101 56-57 0.951887595844731 1101 58-59 1.0297881902541235 1101 60-61 0.43200710832862654 1101 62-63 0.8780844646471024 1101 64-65 0.5751971391339481 1101 66-67 0.3539220755590904 1101 68-69 0.4177742109006175 1101 70-71 -0.046073269288960716 1101 72-73 0.209357210326921 1101 74-75 0.44408747681155347 1101 76-77 0.32304912270377883 1101 78-79 0.2182689587115192 1101 80-81 0.18314687129785057 1101 82-83 0.37145209339201557 1101 84-85 0.1976183253541528 1101 86-87 0.17873841784504663 1101 88-89 0.16668002070921517 1101 90-91 -0.17810369233548684 1101 92-93 -0.10975753749278638 1101 94-95 0.056140604986325116 1101 96-97 -0.08737240058836093 1101 98-99 0.18098218630752072 1101 100-101 0.33568668313404615 1102 1 -0.15636876455483772 1102 2 -0.074494664387597 1102 3 -0.0842247153924447 1102 4 0.060042273306613936 1102 5 0.1687565521493397 1102 6 0.19069851821882366 1102 7 -0.1981704339776158 1102 8 -0.33390838036912385 1102 9 0.09182921498665308 1102 10-11 0.061200344635395254 1102 12-13 0.2574966291701699 1102 14-15 0.4885772529094581 1102 16-17 0.5816078514094372 1102 18-19 0.4745865921676611 1102 20-21 0.4364488072126491 1102 22-23 0.4833233886994748 1102 24-25 0.25295790607939495 1102 26-27 0.33649353847747676 1102 28-29 0.1177703245474433 1102 30-31 0.08664804052443742 1102 32-33 0.5187227692946834 1102 34-35 0.47177887984263833 1102 36-37 0.48128097280194737 1102 38-39 -0.00368770914919736 1102 40-41 0.5072876252206484 1102 42-43 0.586192833681821 1102 44-45 0.5768163378024909 1102 46-47 0.7737786047876725 1102 48-49 0.7614769771662253 1102 50-51 0.6020987643952509 1102 52-53 0.5474324366507233 1102 54-55 0.641857605665578 1102 56-57 0.41043737939451574 1102 58-59 0.5711301815961178 1102 60-61 0.2102538615753744 1102 62-63 0.44442645598909536 1102 64-65 0.38937462831142966 1102 66-67 0.27232034395736804 1102 68-69 0.3110642541906543 1102 70-71 0.32232499910930557 1102 72-73 0.2826256085953176 1102 74-75 -0.0866484539243828 1102 76-77 0.4822482569029205 1102 78-79 0.1289832444258039 1102 80-81 0.2950516332026112 1102 82-83 0.5782702752102011 1102 84-85 0.439826117561946 1102 86-87 0.6066604957671231 1102 88-89 0.40022980425899846 1102 90-91 0.43596557173377803 1102 92-93 0.7183809906457412 1102 94-95 0.03871636256207722 1102 96-97 0.1460691578531943 1102 98-99 0.5730817534070844 1102 100-101 0.5373100597574272 1103 1 -0.14834737418050636 1103 2 -0.02776688236570024 1103 3 0.17386033681025737 1103 4 0.391083780818704 1103 5 0.26959688828379313 1103 6 0.3495984418246252 1103 7 0.2004799352609936 1103 8 0.3423075605526975 1103 9 0.19165096186722508 1103 10-11 0.2332782667133202 1103 12-13 0.24037159733925506 1103 14-15 0.5441540290744697 1103 16-17 0.3357458702533407 1103 18-19 0.22503222496624176 1103 20-21 0.16314857803006788 1103 22-23 0.16826100010767675 1103 24-25 0.22953035324007232 1103 26-27 0.4209091228930646 1103 28-29 0.23484442691566443 1103 30-31 0.34632463843632877 1103 32-33 0.25497181436725924 1103 34-35 0.36590925926714135 1103 36-37 0.38356006625750894 1103 38-39 0.2307151429007135 1103 40-41 0.3734068001633588 1103 42-43 0.2763507150161715 1103 44-45 0.15989502382235798 1103 46-47 0.26677580366722253 1103 48-49 0.37091166013032506 1103 50-51 0.41556960727785963 1103 52-53 0.34721598643427143 1103 54-55 0.33825434617996564 1103 56-57 0.06157055993946159 1103 58-59 0.2102312765795631 1103 60-61 0.35521184476865386 1103 62-63 0.41603328053709276 1103 64-65 0.4250252521973579 1103 66-67 0.3009045048944685 1103 68-69 0.24466496720313557 1103 70-71 0.3339114518722255 1103 72-73 0.20139311559812256 1103 74-75 0.18289572738449777 1103 76-77 0.05259457724923777 1103 78-79 0.057745658482332374 1103 80-81 0.29575191331466044 1103 82-83 0.14511519499630054 1103 84-85 0.0782905942617198 1103 86-87 0.4336276462636839 1103 88-89 0.1865807082569617 1103 90-91 0.17151433669430816 1103 92-93 -0.3510104022750653 1103 94-95 0.3531421328702038 1103 96-97 0.2367872632771828 1103 98-99 0.36618081121202906 1103 100-101 0.47619470452442414 1104 1 -0.3045387763612126 1104 2 -0.12919753727229022 1104 3 0.01770365926320494 1104 4 -0.06412144176619705 1104 5 0.05600566667118301 1104 6 -0.014142095712699643 1104 7 -0.17239318092763511 1104 8 -0.11942589315936658 1104 9 0.050703673861107745 1104 10-11 0.012203886548029175 1104 12-13 0.249330552822272 1104 14-15 0.12671184558950443 1104 16-17 0.25535834334174723 1104 18-19 0.13790811912555512 1104 20-21 0.17228115668135757 1104 22-23 -0.07590526143826537 1104 24-25 0.04408344720493318 1104 26-27 0.09151124804064636 1104 28-29 0.2863063339925418 1104 30-31 0.1963489456798868 1104 32-33 0.22149726752372345 1104 34-35 0.20338061144437347 1104 36-37 0.2768339047185151 1104 38-39 0.375100169638678 1104 40-41 0.2924115921628001 1104 42-43 0.25728689113951475 1104 44-45 0.4962378254057995 1104 46-47 0.2780092226546529 1104 48-49 0.5902845332465105 1104 50-51 0.5505442582952895 1104 52-53 0.3776174031993236 1104 54-55 0.6174577630839124 1104 56-57 0.7425900751835783 1104 58-59 0.3835071985185934 1104 60-61 0.4921310752707697 1104 62-63 0.4861423158867737 1104 64-65 0.4849078829355946 1104 66-67 0.31797166233595675 1104 68-69 0.6954601613138323 1104 70-71 0.3245878877358308 1104 72-73 0.2624563838806395 1104 74-75 0.27590565862973193 1104 76-77 0.37904085824096967 1104 78-79 0.4465747438354839 1104 80-81 0.46703116881851514 1104 82-83 0.5824025066151606 1104 84-85 0.6765439451888646 1104 86-87 0.6217136244566177 1104 88-89 0.6104816736017753 1104 90-91 0.8165244068380666 1104 92-93 0.6323912228377893 1104 94-95 1.0394050677962383 1104 96-97 0.9822556985851882 1104 98-99 0.7477177234976011 1104 100-101 0.5181246996629731 1105 1 -0.12804768804098998 1105 2 -0.12315684557989925 1105 3 -0.08385961864180302 1105 4 -0.01730608115017418 1105 5 -0.16974029237159982 1105 6 -0.04525829605124443 1105 7 -0.12139580298611463 1105 8 -0.08152221232030854 1105 9 -0.02541899864710473 1105 10-11 0.11982445145228127 1105 12-13 -0.027852559793480225 1105 14-15 0.057476225422881555 1105 16-17 0.013465150736621467 1105 18-19 0.17791940393421157 1105 20-21 0.2116535221763982 1105 22-23 0.2973065942489441 1105 24-25 0.06973149393731859 1105 26-27 0.4220565698236385 1105 28-29 0.06415325888218604 1105 30-31 0.03785546763547387 1105 32-33 0.270926388968185 1105 34-35 0.3968818892829944 1105 36-37 0.4502216706583013 1105 38-39 0.5061699031180567 1105 40-41 0.14036539082853494 1105 42-43 0.20732671987594387 1105 44-45 0.4212851220303193 1105 46-47 0.6928835961818223 1105 48-49 0.7117455840613403 1105 50-51 0.777319126362606 1105 52-53 0.707084030037251 1105 54-55 0.7817678961782804 1105 56-57 0.6307211117264373 1105 58-59 0.7305731482680002 1105 60-61 0.6568975793034326 1105 62-63 0.5783126501162528 1105 64-65 0.6166995122628265 1105 66-67 0.44441130520495165 1105 68-69 0.346087463912653 1105 70-71 0.2673257814594834 1105 72-73 0.13517867801586192 1105 74-75 0.3145146400097971 1105 76-77 0.4552050190163044 1105 78-79 0.3454595392153479 1105 80-81 0.39459265443038305 1105 82-83 0.6823228491422952 1105 84-85 0.6255299719405052 1105 86-87 0.5181506120765178 1105 88-89 0.6272678264777412 1105 90-91 0.5430171546889788 1105 92-93 0.6066353066109009 1105 94-95 0.9202424669797509 1105 96-97 0.848671776118465 1105 98-99 0.6032804703527894 1105 100-101 0.7433810971537618 1106 1 0.17854338212201526 1106 2 0.16803143696203549 1106 3 0.054812717762636964 1106 4 0.31489330462822807 1106 5 0.165955431701164 1106 6 0.185452783561324 1106 7 0.3357740529080502 1106 8 0.34762968940423633 1106 9 0.3810067041641432 1106 10-11 0.2538665020074333 1106 12-13 0.26964330602272213 1106 14-15 0.21684310470471502 1106 16-17 0.18112402151384543 1106 18-19 0.06676891964410459 1106 20-21 0.07841468447264077 1106 22-23 0.32610413736257726 1106 24-25 -0.0855956971800893 1106 26-27 0.013906321772608976 1106 28-29 -0.15269058708993555 1106 30-31 -0.21151849881857743 1106 32-33 -0.09278728927420588 1106 34-35 -0.2303092281278225 1106 36-37 -0.14935309900860005 1106 38-39 0.00522494682228114 1106 40-41 0.39623593181601535 1106 42-43 0.09749917439992117 1106 44-45 0.06647765687557694 1106 46-47 -0.045269014259943674 1106 48-49 -0.1575197124186971 1106 50-51 -0.07630714342241873 1106 52-53 -0.07281202477020798 1106 54-55 -0.18905657818978483 1106 56-57 -0.1994938658308527 1106 58-59 -0.09579113238402925 1106 60-61 -0.37293941573555145 1106 62-63 -0.06884066904273567 1106 64-65 -0.054526568530938846 1106 66-67 -0.19839521499348933 1106 68-69 -0.24791206360919205 1106 70-71 0.01738484122797246 1106 72-73 -0.07927915330944302 1106 74-75 0.09774166575985532 1106 76-77 0.06533967528845608 1106 78-79 -0.08721232471094709 1106 80-81 0.10373510659196938 1106 82-83 0.07844852832963767 1106 84-85 0.2338928351580769 1106 86-87 0.184888150465369 1106 88-89 0.10562832730457927 1106 90-91 0.42305495294080586 1106 92-93 0.37419968175854734 1106 94-95 0.02499087236600417 1106 96-97 0.20723544254889248 1106 98-99 0.4516149848814912 1106 100-101 0.5605084300146181 1107 1 -0.0692386815231174 1107 2 -0.12333789765706271 1107 3 0.005069869803783433 1107 4 0.06018420787477652 1107 5 -0.0051310356726688156 1107 6 0.13427952737360016 1107 7 0.14165640584922556 1107 8 0.12874508638270044 1107 9 0.0577826604482965 1107 10-11 0.05499957818873469 1107 12-13 0.0692736499635842 1107 14-15 0.2602134036439665 1107 16-17 0.08500541013486895 1107 18-19 0.050335297014726166 1107 20-21 0.010751553646542789 1107 22-23 0.019152996250390686 1107 24-25 0.1841728659828803 1107 26-27 0.07225572710851935 1107 28-29 0.15976521490298978 1107 30-31 0.10476243978637001 1107 32-33 0.3240240254156035 1107 34-35 0.35054901680949513 1107 36-37 0.4102338505253087 1107 38-39 0.08076335890842756 1107 40-41 0.02442622432646857 1107 42-43 0.1475795344127775 1107 44-45 -0.0036605623465320036 1107 46-47 0.11852879994770404 1107 48-49 0.2802655656269408 1107 50-51 0.3171917315373989 1107 52-53 0.4631410449822795 1107 54-55 0.3776020524756021 1107 56-57 0.7058741830280368 1107 58-59 0.43522783257900954 1107 60-61 0.513825290146805 1107 62-63 0.6945151650942023 1107 64-65 0.8819408053038416 1107 66-67 0.27609048092421773 1107 68-69 0.5095774895891338 1107 70-71 0.5628686085053758 1107 72-73 0.34039297783809985 1107 74-75 0.31614410870424337 1107 76-77 0.11406998708530125 1107 78-79 0.0552659780855862 1107 80-81 0.3364255598224375 1107 82-83 0.2833745971178061 1107 84-85 0.348437997485302 1107 86-87 0.5771735892310375 1107 88-89 0.3756218985035531 1107 90-91 0.44052521973604897 1107 92-93 0.3824928350854542 1107 94-95 0.7777874008134447 1107 96-97 0.7023816977722923 1107 98-99 0.8692459717024512 1107 100-101 0.6543528530297245 1108 1 0.1754540888507492 1108 2 0.17920367564167705 1108 3 0.22817990636469432 1108 4 0.42490882162639565 1108 5 0.10092510302293078 1108 6 0.1652383668593913 1108 7 0.44021755692835995 1108 8 0.2437645462246678 1108 9 0.22728967778524378 1108 10-11 0.1505444537636791 1108 12-13 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0.55857819330474 2316 96-97 0.6109436615878003 2316 98-99 0.8746821392043636 2316 100-101 0.8102392031528893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4785.0 3 539.0 4 405.0 5 327.0 6 347.0 7 372.0 8 381.0 9 457.0 10 419.0 11 530.0 12 594.0 13 777.0 14 878.0 15 938.0 16 1103.0 17 1371.0 18 1409.0 19 917.0 20 801.0 21 793.0 22 889.0 23 941.0 24 1100.0 25 1259.0 26 1549.0 27 1731.0 28 2261.0 29 2601.0 30 3202.0 31 4217.0 32 5394.0 33 7277.0 34 10492.0 35 16158.0 36 28856.0 37 56643.0 38 85560.0 39 29274.0 40 74.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.404899941194934 20.73329557302471 14.453780299941915 23.408024185838443 2 15.982494624276622 22.059226761721966 39.14356428499271 22.814714329008705 3 17.83558747876029 26.376928710320968 31.430086618348945 24.3573971925698 4 13.350962891343718 19.27297915479137 37.89188487153031 29.484173082334607 5 14.077774972042857 37.189856065798494 34.688503300746724 14.043865661411925 6 29.47604946999015 37.114755517404305 19.220656498075805 14.188538514529736 7 25.267007888500537 33.27634279448418 23.9955417527837 17.461107564231586 8 24.506621656712543 34.28222106812083 22.12736951441184 19.083787760754785 9 25.202779951743963 17.104204911440434 22.272794486260732 35.420220650554874 10-11 23.583597625070542 26.60779226399003 29.61503857547505 20.193571535464375 12-13 24.480808488844325 25.28439215459969 28.904486074342312 21.330313282213677 14-15 22.35727632803538 27.00567070927154 26.846587834669243 23.79046512802384 16-17 21.46861674110525 29.179143246772927 27.920060897736537 21.432179114385285 18-19 21.337816290160823 28.395406893267626 29.443394252483785 20.823382564087765 20-21 22.286451193997546 27.464468653055334 29.234723324435468 21.01435682851165 22-23 22.129660356668783 27.43748917873838 28.94146418883823 21.491386275754603 24-25 21.786620453606908 27.76155792351402 28.97807704861568 21.47374457426339 26-27 21.797882086462234 27.507628384081023 29.15485439344428 21.539635136012464 28-29 21.686101663125925 27.641939195452466 29.221926859970644 21.45003228145097 30-31 22.171690304664185 27.546907825334284 28.828499039753314 21.45290283024822 32-33 21.931194414189576 27.687413893837864 28.7756875870078 21.605704104964765 34-35 22.044413824447624 27.71364211035102 28.666990100402728 21.574953964798627 36-37 22.026237857686496 27.646059451796862 28.674616658190043 21.6530860323266 38-39 22.05954220773951 27.79051126086729 28.60994245587713 21.540004075516066 40-41 22.11984974148394 27.763020030260897 28.663633363449957 21.453496864805206 42-43 22.189882639775522 27.668556548585116 28.360671701542923 21.780889110096442 44-45 22.237512512286838 27.843017016710103 28.105436870440343 21.814033600562716 46-47 22.132627315044427 27.88856405474768 28.105707963085536 21.873100667122355 48-49 22.153981017521453 27.942338286382185 28.179348708347312 21.72433198774905 50-51 22.240177176928043 28.0399080930756 27.838636257065254 21.8812784729311 52-53 22.2282733162981 27.97649210061727 27.847195047399904 21.948039535684725 54-55 22.264936151491 27.95666739993581 27.847037652769412 21.931358795803778 56-57 22.14910105449581 28.04179749424238 27.85639956500019 21.952701886261615 58-59 22.23851232307057 27.812972811160463 27.967338047243693 21.98117681852527 60-61 22.237810914769724 27.983005519960034 27.91177441198269 21.86740915328754 62-63 22.04369716379584 28.185319830347094 27.8136631178049 21.957319888052165 64-65 22.091018292396985 28.019363752123773 27.700482290174914 22.189135665304327 66-67 21.923823986120304 28.107827347834636 27.772377312756657 22.195971353288403 68-69 22.12598950658589 28.058790533923233 27.78265755000965 22.032562409481226 70-71 22.131231797156072 27.833151492745912 27.80970758230165 22.225909127796363 72-73 21.999437326869806 27.771886542012926 28.025089450600184 22.20358668051708 74-75 22.110809187999127 27.911459394546995 27.708788246515915 22.268943170937966 76-77 22.029123517041622 27.961317736344522 27.80206541068343 22.207493335930426 78-79 21.90852360855607 28.007971720232298 27.64906395411752 22.43444071709411 80-81 21.98603688988044 28.197835375256332 27.562984592243637 22.25314314261959 82-83 22.100816691677245 28.103175862865058 27.52258166916772 22.27342577628997 84-85 22.12106565478037 28.08886227717701 27.487393503055134 22.30267856498748 86-87 21.905503971603576 28.004855384570988 27.680020777871565 22.40961986595387 88-89 22.064658312954023 27.95770482105886 27.756075919233012 22.2215609467541 90-91 22.04503440253217 27.934495414498663 27.702740479561378 22.31772970340779 92-93 22.019899205258355 27.75499013337085 27.7539078705758 22.471202790794994 94-95 21.987221219340213 27.75462432801395 28.006016089794105 22.252138362851728 96-97 22.025499964838826 27.70375899990804 27.845665889514574 22.425075145738557 98-99 21.84787881082855 27.789588709110287 27.736574757785746 22.625957722275416 100-101 21.84011097742028 27.86792388161206 27.837076776820297 22.454888364147365 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 571.0 1 332.0 2 83.0 3 68.5 4 58.0 5 58.5 6 71.0 7 113.0 8 168.0 9 209.5 10 180.5 11 126.5 12 139.0 13 150.0 14 157.0 15 178.5 16 181.0 17 188.5 18 230.0 19 238.0 20 241.0 21 278.5 22 339.0 23 417.0 24 516.5 25 651.5 26 872.0 27 1174.0 28 1484.0 29 2009.5 30 2843.0 31 3916.0 32 5183.0 33 6446.5 34 7692.5 35 8922.5 36 10062.5 37 10895.5 38 11099.5 39 10902.5 40 10691.0 41 10421.0 42 10190.0 43 10220.0 44 10242.5 45 10163.5 46 10055.0 47 9969.5 48 9831.5 49 9586.0 50 9310.5 51 8823.5 52 8335.5 53 7958.5 54 7502.5 55 6915.5 56 6144.0 57 5370.0 58 4848.0 59 4341.0 60 3586.5 61 2927.0 62 2458.0 63 1917.0 64 1408.0 65 1075.5 66 833.0 67 675.5 68 539.0 69 405.5 70 347.0 71 289.5 72 229.5 73 178.0 74 139.0 75 114.0 76 92.5 77 74.0 78 57.5 79 47.5 80 37.5 81 26.0 82 20.0 83 15.5 84 12.0 85 8.0 86 5.0 87 6.0 88 5.5 89 3.5 90 2.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1563282316539455 2 0.16173128113507262 3 0.15416701186149462 4 0.15668843495268728 5 0.14804355578288386 6 0.1303935941445352 7 0.13759766011937136 8 0.12571095126089166 9 0.12607115455963344 10-11 0.11076251436310655 12-13 0.13075379744327698 14-15 0.14660274258791664 16-17 0.15668843495268728 18-19 0.15236599536778558 20-21 0.14444152279546577 22-23 0.14011908321056404 24-25 0.1276920694039716 26-27 0.13219461063824423 28-29 0.1339956271319533 30-31 0.12553084961152075 32-33 0.12499054466340803 34-35 0.13957877826245133 36-37 0.13975887991182223 38-39 0.1280522727027134 40-41 0.1316543056901315 42-43 0.1278721710533425 44-45 0.1417399980549022 46-47 0.1388583716649677 48-49 0.15056497887407655 50-51 0.13831806671685498 52-53 0.12697166280648797 54-55 0.1167058687923464 56-57 0.13669715187251685 58-59 0.12841247600145522 60-61 0.1276920694039716 62-63 0.12571095126089166 64-65 0.14480172609420758 66-67 0.13741755847000045 68-69 0.14390121784735305 70-71 0.13093389909264788 72-73 0.13435583043069507 74-75 0.1168859704417173 76-77 0.13975887991182223 78-79 0.14083948980804767 80-81 0.14065938815867676 82-83 0.145882335990433 84-85 0.1379578634181132 86-87 0.14516192939294936 88-89 0.13651705022314595 90-91 0.13993898156119314 92-93 0.15272619866652737 94-95 0.13129410239138972 96-97 0.11760637703920093 98-99 0.1208482067278772 100-101 0.16119097618695993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 277621.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.99357440313538 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92952736436074 56.153611794685055 2 16.03104025533632 23.403258592751 3 4.469361951546767 9.787041124343139 4 1.4008723770101863 4.090187283223607 5 0.5320094540851316 1.9416635834967246 6 0.2515293658310491 1.1016016487617286 7 0.1301549208304041 0.6650331028297809 8 0.08684627715004399 0.5071376154229642 9 0.04045898177948522 0.2657921127116358 >10 0.12472588205518428 1.3436730473656402 >50 4.961671449590927E-4 0.02897361072957024 >100 0.001984668579836371 0.2908226176980613 >500 9.923342899181855E-4 0.42120386598112736 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.21432096275137688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 568 0.20459547368534803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 282 0.10157733024519038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 3.602032987418099E-4 0.0 0.0 14-15 0.0 0.0 3.602032987418099E-4 0.0 0.0 16-17 0.0 0.0 3.602032987418099E-4 0.0 0.0 18-19 0.0 0.0 3.602032987418099E-4 0.0 0.0 20-21 0.0 0.0 3.602032987418099E-4 0.0 0.0 22-23 0.0 0.0 3.602032987418099E-4 0.0 0.0 24-25 0.0 0.0 3.602032987418099E-4 5.403049481127149E-4 0.0 26-27 0.0 0.0 3.602032987418099E-4 0.0012607115455963345 0.0 28-29 0.0 0.0 3.602032987418099E-4 0.0023413214418217644 0.0 30-31 0.0 0.0 3.602032987418099E-4 0.005403049481127149 0.0 32-33 0.0 0.0 3.602032987418099E-4 0.011346403910367012 0.0 34-35 0.0 0.0 3.602032987418099E-4 0.019270876482686827 0.0 36-37 0.0 0.0 3.602032987418099E-4 0.031157585341166555 0.0 38-39 0.0 0.0 3.602032987418099E-4 0.04484531069335533 0.0 40-41 7.204065974836198E-4 0.0 3.602032987418099E-4 0.05403049481127148 0.0 42-43 7.204065974836198E-4 0.0 3.602032987418099E-4 0.06915903335842749 0.0 44-45 7.204065974836198E-4 0.0 3.602032987418099E-4 0.0875294015942598 0.0 46-47 7.204065974836198E-4 0.0 3.602032987418099E-4 0.11346403910367012 0.0 48-49 7.204065974836198E-4 0.0 3.602032987418099E-4 0.1457022343410621 0.0 50-51 7.204065974836198E-4 0.0 3.602032987418099E-4 0.17307768504543963 0.0 52-53 7.204065974836198E-4 0.0 3.602032987418099E-4 0.19883222090547903 0.0 54-55 7.204065974836198E-4 0.0 3.602032987418099E-4 0.23773417716959452 0.0 56-57 7.204065974836198E-4 0.0 3.602032987418099E-4 0.27789684497930633 0.0 58-59 7.204065974836198E-4 0.0 3.602032987418099E-4 0.32328246062077437 0.0 60-61 7.204065974836198E-4 0.0 3.602032987418099E-4 0.3733507191458859 0.0 62-63 7.204065974836198E-4 0.0 3.602032987418099E-4 0.41693531829364494 0.0 64-65 7.204065974836198E-4 0.0 3.602032987418099E-4 0.4733071345467382 0.0 66-67 7.204065974836198E-4 0.0 3.602032987418099E-4 0.5675002971677214 0.0 68-69 7.204065974836198E-4 0.0 3.602032987418099E-4 0.6728597620497008 0.0 70-71 7.204065974836198E-4 0.0 3.602032987418099E-4 0.7859635978546291 0.0 72-73 7.204065974836198E-4 0.0 3.602032987418099E-4 0.9109541425180372 0.0 74-75 7.204065974836198E-4 0.0 3.602032987418099E-4 1.034503873986478 0.0 76-77 7.204065974836198E-4 0.0 3.602032987418099E-4 1.1787652951325729 0.0 78-79 7.204065974836198E-4 0.0 3.602032987418099E-4 1.339596068020791 0.0 80-81 7.204065974836198E-4 0.0 3.602032987418099E-4 1.5067303986369907 0.0 82-83 7.204065974836198E-4 0.0 3.602032987418099E-4 1.691874894190281 0.0 84-85 7.204065974836198E-4 0.0 3.602032987418099E-4 1.89268823323884 0.0 86-87 7.204065974836198E-4 0.0 3.602032987418099E-4 2.1585182677102956 0.0 88-89 7.204065974836198E-4 0.0 3.602032987418099E-4 2.466492088134543 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 425 0.0 46.95321 1 GTATCAA 680 0.0 33.538006 1 GTGGTAT 240 0.0 29.695108 1 ACTATAG 65 0.005834361 29.232988 3 ATGCGGA 50 4.9520717E-5 28.497026 20-21 CCGTTCT 85 6.3987356E-4 27.938261 9 TATCAAC 865 0.0 26.914412 2 TGGTATC 250 0.0 26.606817 2 ATAAGAC 90 8.9264044E-4 26.390894 3 ATCAACG 910 0.0 24.53483 3 TCAACGC 925 0.0 24.136969 4 CAACGCA 950 0.0 23.997494 5 AACGCAG 960 0.0 23.74752 6 ACGCAGA 1060 0.0 21.059122 7 CGCAGAG 1080 0.0 20.66914 8 TAGACTG 140 4.842387E-4 20.355017 5 GCCTAGA 120 0.004747508 19.79674 1 GTAGACT 125 0.00601722 19.001444 4 ATTATAC 125 0.00601722 19.001444 3 GAGTACT 675 0.0 18.65293 12-13 >>END_MODULE