FastQCFastQC Report
Fri 10 Feb 2017
SRR2031736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277621
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT25120.9048306864394263No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT9500.3421931338047194RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.29716772146199316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.2852810126035134No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT7780.2802381664211281RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG7640.2751953202387427RNA PCR Primer, Index 27 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.12895278094956794No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT3360.12102830837724812RNA PCR Primer, Index 27 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5050.064.90851
GTATCAA8900.037.3638731
TCAACGC9800.033.441724
ATCAACG10000.032.772893
TATCAAC10150.032.7565083
CAACGCA10200.032.130285
AACGCAG10550.031.0643466
ACGCAGA11700.028.0110157
GTAACAC856.397258E-427.9393773
CGCAGAG12150.026.5826478
TATACAC10850.025.3900683
CTTATAC11350.024.2759361
ACACATC11500.023.9549796
TACACAT11750.023.4452975
CACATCT11800.023.3459537
TTATACA11600.023.3390142
ACATCTC11700.023.1395348
GCAGAGT14550.022.1978829
CATCTCC12250.022.1006159
CAGAGTA13650.021.92166110-11
Produced by FastQC (version 0.11.5)