##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031736_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 277621 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75990288919066 34.0 31.0 34.0 31.0 34.0 2 32.92485798984947 34.0 31.0 34.0 31.0 34.0 3 32.97800238454584 34.0 31.0 34.0 31.0 34.0 4 36.3776983729617 37.0 37.0 37.0 35.0 37.0 5 36.34541695332846 37.0 37.0 37.0 35.0 37.0 6 36.368268250600636 37.0 37.0 37.0 35.0 37.0 7 36.31347052276305 37.0 37.0 37.0 35.0 37.0 8 36.30754517849874 37.0 37.0 37.0 35.0 37.0 9 38.158291339632086 39.0 39.0 39.0 37.0 39.0 10-11 38.12667269406853 39.0 39.0 39.0 37.0 39.0 12-13 38.07799842230955 39.0 39.0 39.0 35.0 39.0 14-15 39.566068489055226 41.0 39.5 41.0 37.0 41.0 16-17 39.5393360012391 41.0 39.0 41.0 37.0 41.0 18-19 39.55145864325826 41.0 39.5 41.0 37.0 41.0 20-21 39.53513783179227 41.0 39.5 41.0 37.0 41.0 22-23 39.44387492300655 41.0 39.0 41.0 36.0 41.0 24-25 39.380288234679654 41.0 39.0 41.0 36.0 41.0 26-27 39.236473465624 41.0 39.0 41.0 36.0 41.0 28-29 39.12921933139064 40.5 39.0 41.0 36.0 41.0 30-31 38.9462360556298 40.0 38.5 41.0 35.0 41.0 32-33 38.7435154401144 40.0 38.0 41.0 35.0 41.0 34-35 38.59452635067232 40.0 38.0 41.0 35.0 41.0 36-37 38.475313466920724 40.0 38.0 41.0 34.5 41.0 38-39 38.523443831698614 40.0 38.0 41.0 35.0 41.0 40-41 38.440530435377724 40.0 38.0 41.0 35.0 41.0 42-43 38.33288548056523 40.0 38.0 41.0 34.0 41.0 44-45 38.1551107445042 40.0 38.0 41.0 34.0 41.0 46-47 37.93876003616441 40.0 38.0 41.0 33.0 41.0 48-49 37.681221881629995 40.0 38.0 41.0 33.0 41.0 50-51 37.49277432182724 40.0 37.5 41.0 33.0 41.0 52-53 37.57675752194538 40.0 37.5 41.0 33.0 41.0 54-55 37.48568011785852 40.0 37.0 41.0 33.0 41.0 56-57 37.253928917481026 40.0 37.0 41.0 33.0 41.0 58-59 37.01775442059498 40.0 36.5 41.0 32.5 41.0 60-61 36.68975329676069 39.5 36.0 41.0 31.5 41.0 62-63 36.37112646377616 39.0 35.0 41.0 31.0 41.0 64-65 35.98508037936611 39.0 35.0 41.0 31.0 41.0 66-67 35.53842828892627 38.5 35.0 40.5 29.5 41.0 68-69 35.042014112765244 37.5 35.0 40.0 29.0 41.0 70-71 34.673599979828616 37.0 35.0 39.5 28.5 41.0 72-73 34.29570889810209 36.5 35.0 39.0 28.0 41.0 74-75 33.93139928175462 36.0 34.0 39.0 28.0 41.0 76-77 33.01226132028917 35.0 33.5 37.0 26.0 39.0 78-79 33.200523375393075 35.0 34.0 37.0 27.0 39.0 80-81 32.958457753556104 35.0 34.0 37.0 27.0 39.0 82-83 32.674437452498196 35.0 34.0 36.5 26.5 38.5 84-85 32.36099574599905 35.0 34.0 36.0 26.0 37.0 86-87 32.11583597782588 35.0 34.0 36.0 26.0 37.0 88-89 31.88027742858069 35.0 34.0 35.0 25.5 36.5 90-91 31.67082101137882 35.0 34.0 35.0 25.0 36.0 92-93 31.514228030300302 35.0 33.5 35.0 24.5 36.0 94-95 31.33602825434675 35.0 33.0 35.0 24.0 36.0 96-97 31.181198468415573 35.0 33.0 35.0 23.0 35.5 98-99 30.978735398258777 35.0 33.0 35.0 19.5 35.0 100-101 30.300126791561155 34.5 32.0 35.0 9.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2010535125527113 1101 2 0.11917969611931056 1101 3 0.1732032390737288 1101 4 0.056886904930536275 1101 5 0.15025241053715632 1101 6 0.10248372445820308 1101 7 0.12420531996151851 1101 8 0.13383762665885968 1101 9 0.20123964875332945 1101 10-11 0.17216344927000904 1101 12-13 0.21656906187641312 1101 14-15 0.10208636196612275 1101 16-17 0.17811293233359038 1101 18-19 0.14159519783038377 1101 20-21 0.03845177877675354 1101 22-23 -0.11379023754591344 1101 24-25 0.09957861880634056 1101 26-27 0.2738450494877682 1101 28-29 -0.04933337632963486 1101 30-31 0.0984641966624622 1101 32-33 0.3501939480724303 1101 34-35 -0.517894012074251 1101 36-37 0.013733246200942517 1101 38-39 -0.25855751919652903 1101 40-41 -0.645618648172352 1101 42-43 -0.31552522603566047 1101 44-45 -0.15515113828977434 1101 46-47 0.08017562397715494 1101 48-49 -0.13796479513620596 1101 50-51 -0.07244406798577074 1101 52-53 0.14074484769957252 1101 54-55 0.3709288494850327 1101 56-57 0.14110863110492033 1101 58-59 0.34338059138355703 1101 60-61 0.1529037089004177 1101 62-63 -0.2279491591529066 1101 64-65 -0.1591261820509402 1101 66-67 -0.28301346428153096 1101 68-69 0.12077269499665277 1101 70-71 0.025141756225110612 1101 72-73 -0.0818188086187206 1101 74-75 -0.41121190640914307 1101 76-77 -0.4796190565490974 1101 78-79 -0.15913446963987354 1101 80-81 -0.03679708085412159 1101 82-83 -0.053701461633529846 1101 84-85 -0.2509375136151277 1101 86-87 -0.14320344180883637 1101 88-89 0.04464881384249253 1101 90-91 -0.10681863907212374 1101 92-93 -0.2912632417999035 1101 94-95 -0.2743519224384947 1101 96-97 -0.2504265662891818 1101 98-99 0.07548815747178494 1101 100-101 0.05341137788940742 1102 1 0.12356433506353426 1102 2 0.1364957134353233 1102 3 0.19419891006940304 1102 4 0.18221158025521333 1102 5 0.25133466161940277 1102 6 0.178241300215781 1102 7 0.16230055805675647 1102 8 0.19336143618266988 1102 9 0.22569852321220196 1102 10-11 0.24348379559035038 1102 12-13 0.2372400575474103 1102 14-15 0.15143701131678 1102 16-17 0.4008402050608595 1102 18-19 0.26778567402086395 1102 20-21 0.3366119519369306 1102 22-23 0.21705391829823384 1102 24-25 0.16581238504010543 1102 26-27 0.14971085035356424 1102 28-29 0.25488740289114986 1102 30-31 -0.06484749164922476 1102 32-33 0.14034546322394448 1102 34-35 0.19953023034999262 1102 36-37 0.18375489122259125 1102 38-39 -0.031825917464928466 1102 40-41 0.12234671979301481 1102 42-43 0.1948643843539486 1102 44-45 0.055346697208058515 1102 46-47 0.4030111218126535 1102 48-49 0.10229494512353199 1102 50-51 0.1501749796332703 1102 52-53 0.17884008579481048 1102 54-55 0.7030717066278882 1102 56-57 0.6619960769923736 1102 58-59 0.7838567818597468 1102 60-61 0.7618863928831061 1102 62-63 0.825189368985626 1102 64-65 0.5676054196806604 1102 66-67 0.4130904318223614 1102 68-69 0.3593008335247916 1102 70-71 0.5882369943203543 1102 72-73 0.7255404987405925 1102 74-75 0.6965803013830651 1102 76-77 0.5491688222387836 1102 78-79 0.8342637987583927 1102 80-81 0.8285708845138444 1102 82-83 0.810584252652184 1102 84-85 0.8849932223156074 1102 86-87 0.9935930949876948 1102 88-89 0.8648869090805817 1102 90-91 0.870995213741736 1102 92-93 1.1949921694555066 1102 94-95 1.2011892031026328 1102 96-97 1.0581232172606043 1102 98-99 1.2767868587704818 1102 100-101 1.4014633259413536 1103 1 0.21014442164361924 1103 2 0.21734623546231546 1103 3 0.23825289529985838 1103 4 0.1650228865959278 1103 5 0.20384293765709316 1103 6 0.3167694387439184 1103 7 0.23551166067962015 1103 8 0.29522036157100473 1103 9 0.09990275035760732 1103 10-11 0.2508685676810103 1103 12-13 0.26028563941652294 1103 14-15 0.3447143222411455 1103 16-17 0.1865544907751442 1103 18-19 0.3383866416806498 1103 20-21 0.6042462783947826 1103 22-23 0.49684765397432784 1103 24-25 0.22005226280939638 1103 26-27 0.4729856148048057 1103 28-29 0.40232819739076575 1103 30-31 0.39046190483664134 1103 32-33 0.41434597251856786 1103 34-35 0.40897764567976935 1103 36-37 0.5381602897456332 1103 38-39 0.6660987069302848 1103 40-41 0.5691254312776053 1103 42-43 0.6299293194188849 1103 44-45 0.7708419862327673 1103 46-47 0.4764132099206222 1103 48-49 0.5148235929462928 1103 50-51 0.23930153934806953 1103 52-53 0.44857525258880315 1103 54-55 0.46758660349572523 1103 56-57 0.2546876990957614 1103 58-59 0.3779489641872189 1103 60-61 0.1147639075253224 1103 62-63 -0.09064969391226185 1103 64-65 0.24031996004226386 1103 66-67 0.14380089782694228 1103 68-69 0.07123106016090475 1103 70-71 0.0161081427797356 1103 72-73 0.21115292552948262 1103 74-75 0.17423499962599465 1103 76-77 0.2574703246579304 1103 78-79 0.3855752324227737 1103 80-81 0.3454412690312836 1103 82-83 0.3992015177400319 1103 84-85 0.3864574443680695 1103 86-87 0.2818538538366937 1103 88-89 0.2551339169746498 1103 90-91 0.006531246336585639 1103 92-93 0.0509254518593778 1103 94-95 0.4998268399755581 1103 96-97 0.44741529772915456 1103 98-99 0.38641252721968655 1103 100-101 0.4656344489360045 1104 1 0.04485516544527002 1104 2 0.07372515066476382 1104 3 0.18022803246216057 1104 4 0.16391169831896235 1104 5 -0.057598829132267326 1104 6 -8.220606657616258E-4 1104 7 0.11428796458961443 1104 8 0.069788039881999 1104 9 -0.03305787190782894 1104 10-11 -0.0987043193250301 1104 12-13 0.07834592138054575 1104 14-15 0.10539214709009315 1104 16-17 0.1363773951434979 1104 18-19 0.1490332143593136 1104 20-21 -0.014234171636466897 1104 22-23 0.3132758781565599 1104 24-25 0.0607356435997346 1104 26-27 0.14223347923983454 1104 28-29 0.24426166499268476 1104 30-31 0.018092295836012795 1104 32-33 -0.1599300188697228 1104 34-35 0.03561838461996558 1104 36-37 0.013939857771191555 1104 38-39 0.05445900972908646 1104 40-41 0.26884416174500103 1104 42-43 0.049350807022186416 1104 44-45 0.02356786997469129 1104 46-47 0.0353409132333482 1104 48-49 0.21740710569023491 1104 50-51 0.290572460939849 1104 52-53 0.47173658323676193 1104 54-55 0.011218105683376223 1104 56-57 0.05288549060905012 1104 58-59 0.41982687237528893 1104 60-61 0.3485648659252192 1104 62-63 0.4354805929132155 1104 64-65 0.35017133861021676 1104 66-67 0.4405402547267343 1104 68-69 0.6154007941702062 1104 70-71 0.41419134300197413 1104 72-73 0.35826383600937817 1104 74-75 0.4474657795412753 1104 76-77 0.534017307087268 1104 78-79 0.8261961088725229 1104 80-81 0.6586574646004166 1104 82-83 0.7698522573747297 1104 84-85 0.6573269491947897 1104 86-87 0.49192052513331674 1104 88-89 0.4452686485532382 1104 90-91 0.2867764022501902 1104 92-93 0.2837367582000958 1104 94-95 0.45581336681770424 1104 96-97 0.6857304585042066 1104 98-99 0.49490964507509005 1104 100-101 0.26332873326130724 1105 1 0.3145620923263479 1105 2 0.17686498272025375 1105 3 0.20264829201276058 1105 4 0.168077120337621 1105 5 0.1225598220011932 1105 6 0.152684527671056 1105 7 0.23380736450332051 1105 8 0.23889421665521127 1105 9 0.24353610731484565 1105 10-11 0.3456848074299188 1105 12-13 0.3217077986416612 1105 14-15 0.417931560943849 1105 16-17 0.3988321729323445 1105 18-19 0.6134279835185978 1105 20-21 0.5907975253996085 1105 22-23 0.5539078274413072 1105 24-25 0.48099519419881887 1105 26-27 0.5919994854132895 1105 28-29 0.3754475656199858 1105 30-31 0.3626025683309564 1105 32-33 0.289122389109302 1105 34-35 0.5496580142127243 1105 36-37 0.6847719464565145 1105 38-39 0.6347885925230798 1105 40-41 0.750000190819982 1105 42-43 0.7022184760453953 1105 44-45 0.8268222096474318 1105 46-47 0.6499729952805495 1105 48-49 0.8373911741956661 1105 50-51 0.980248520550191 1105 52-53 0.8458196925334533 1105 54-55 0.76944108022618 1105 56-57 0.6706540856503764 1105 58-59 0.9477069878786253 1105 60-61 1.0308062280679948 1105 62-63 0.940533863848195 1105 64-65 0.5534970381023996 1105 66-67 0.7747448416695448 1105 68-69 0.7926967548725159 1105 70-71 0.49070400522109736 1105 72-73 0.5375678288402099 1105 74-75 0.9090144535762548 1105 76-77 0.6251089463716824 1105 78-79 0.5140217917694017 1105 80-81 0.4205231089961856 1105 82-83 0.3998764865227358 1105 84-85 0.30368096028045244 1105 86-87 0.25142963595677514 1105 88-89 0.165413691535079 1105 90-91 0.34439530762375625 1105 92-93 0.20980466619571203 1105 94-95 0.31442135247935354 1105 96-97 0.5000206772303528 1105 98-99 0.5373355470300183 1105 100-101 0.6438093333476012 1106 1 0.1317130490945999 1106 2 0.02827060521021707 1106 3 0.07970947793290861 1106 4 0.08799207427099986 1106 5 -0.049658462903131806 1106 6 0.07307195975231906 1106 7 0.09461530213620506 1106 8 -0.12774882610406024 1106 9 0.16264784839682278 1106 10-11 0.14190498224684234 1106 12-13 0.16420720804396893 1106 14-15 0.21866390207307518 1106 16-17 0.014565695256933964 1106 18-19 0.2079944848179096 1106 20-21 0.20676728679814005 1106 22-23 -0.058754586922034946 1106 24-25 0.14218111435001646 1106 26-27 0.1850304327319705 1106 28-29 0.1961657324047721 1106 30-31 0.1439633053968663 1106 32-33 0.2608445719583514 1106 34-35 0.23446784175819602 1106 36-37 0.3733143513702828 1106 38-39 0.3118970262580234 1106 40-41 0.20161842847648614 1106 42-43 0.12208618216398293 1106 44-45 0.21860108987421967 1106 46-47 0.016049064262354307 1106 48-49 -0.3033836899668714 1106 50-51 -0.1749841749376415 1106 52-53 -0.169772086346768 1106 54-55 -0.22162908277878302 1106 56-57 0.11967369349351742 1106 58-59 -0.3100062178856078 1106 60-61 -0.3265526190853194 1106 62-63 -0.09457126253971637 1106 64-65 -0.1886716365963963 1106 66-67 -0.08533075483412489 1106 68-69 -0.0947353263937174 1106 70-71 -0.3518190280886131 1106 72-73 -0.2244213041623908 1106 74-75 -0.15559693314710898 1106 76-77 -0.05065292464178839 1106 78-79 -0.18725389922989422 1106 80-81 -0.2549788990359403 1106 82-83 -0.24309540102746752 1106 84-85 -0.18231006263473404 1106 86-87 -0.23803410134537728 1106 88-89 -0.17063639114859797 1106 90-91 -0.35579368363540453 1106 92-93 -0.12036306354678672 1106 94-95 -0.13896867823172343 1106 96-97 0.04783546579637843 1106 98-99 -0.04215890135174405 1106 100-101 0.1888837486558934 1107 1 0.26752147082394373 1107 2 0.14712306422661214 1107 3 0.10479787394704942 1107 4 -0.029253184488240436 1107 5 -0.015023297361501875 1107 6 -0.08194250504999445 1107 7 0.1514780472342423 1107 8 -0.056549854712230285 1107 9 0.014801496741405629 1107 10-11 0.16933185463513212 1107 12-13 0.04801466545316657 1107 14-15 0.035804692815602834 1107 16-17 0.21231561489692297 1107 18-19 0.22266822316570511 1107 20-21 0.09191675642205865 1107 22-23 0.12026341372084914 1107 24-25 0.17975745636223195 1107 26-27 0.23297321640281865 1107 28-29 0.007335774191979283 1107 30-31 0.071116953741452 1107 32-33 0.02310750992041477 1107 34-35 0.0051685810703077095 1107 36-37 0.11380776184923036 1107 38-39 0.15370030494653975 1107 40-41 -0.056907768887704435 1107 42-43 -0.09138215599094934 1107 44-45 -0.01506171951182722 1107 46-47 -0.1080509035936359 1107 48-49 -0.036250935225631054 1107 50-51 0.06455295138830053 1107 52-53 0.255573461708515 1107 54-55 0.3449974038814574 1107 56-57 0.20549015122414716 1107 58-59 -0.11917529535266169 1107 60-61 -0.09683548633057626 1107 62-63 -0.11222635736452702 1107 64-65 -0.298097866104591 1107 66-67 0.010466416493429165 1107 68-69 -0.23805740932525055 1107 70-71 -0.2871533257420964 1107 72-73 -0.3415058428959199 1107 74-75 -0.44284379910661187 1107 76-77 -0.3167651072197373 1107 78-79 -0.3236650210556675 1107 80-81 0.004037494406681219 1107 82-83 -0.008395947475555943 1107 84-85 0.0793903552373294 1107 86-87 0.03392025416732025 1107 88-89 -0.21097711760311455 1107 90-91 -0.05707944384112551 1107 92-93 -0.13858067846160438 1107 94-95 -0.09126697459944921 1107 96-97 0.029416950849419976 1107 98-99 0.19638979681604596 1107 100-101 0.30195832274782575 1108 1 0.04467679574433703 1108 2 0.03186772751237754 1108 3 0.006525672036453045 1108 4 -0.08206011926829149 1108 5 -0.09719690273949766 1108 6 -0.08096951295186727 1108 7 -0.006795334060718972 1108 8 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-0.32056188285756093 2316 76-77 -0.5567740259320431 2316 78-79 -0.4490897216417551 2316 80-81 -0.5391757902276524 2316 82-83 -0.5235789934281598 2316 84-85 -0.28487533744932847 2316 86-87 -0.4449933617334736 2316 88-89 -0.45091171371285554 2316 90-91 -0.6584554737400445 2316 92-93 -0.7843273020919419 2316 94-95 -0.5703717057639786 2316 96-97 -0.6876262836702445 2316 98-99 -0.5773615834608563 2316 100-101 -0.28811005404653045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 3.0 6 3.0 7 17.0 8 37.0 9 103.0 10 159.0 11 258.0 12 410.0 13 558.0 14 744.0 15 890.0 16 1065.0 17 1494.0 18 1644.0 19 1065.0 20 914.0 21 1162.0 22 1463.0 23 1684.0 24 1954.0 25 2334.0 26 1905.0 27 1450.0 28 1819.0 29 2416.0 30 3085.0 31 3898.0 32 4945.0 33 6640.0 34 9574.0 35 15260.0 36 27014.0 37 56348.0 38 93108.0 39 32149.0 40 49.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.39651116290967 21.019847174236503 14.991119389273374 23.592522273580453 2 15.530165225253134 21.81679339819394 40.02362933639746 22.629412040155465 3 17.109121166494965 26.70323970347744 32.56128925357866 23.62634987644893 4 12.935963253174817 20.013689993695397 37.4932901017743 29.55705665135549 5 13.36426278991863 37.12723461121457 35.7689079716592 13.739594627207596 6 28.707122299826022 37.86565137363528 19.102661542174403 14.324564784364297 7 24.367392956584695 33.51619654132792 23.869952201022258 18.246458301065122 8 23.911015377078822 34.7574571087922 22.59051008389135 18.741017430237626 9 24.50318960021036 16.557104829966033 22.076499976586785 36.86320559323683 10-11 23.044006037007286 26.648200244217833 29.8790437322825 20.428749986492374 12-13 24.083012452228036 24.972534498470935 29.334236242935514 21.61021680636551 14-15 21.855335151159313 26.90898743250691 26.593449342809084 24.6422280735247 16-17 21.334106582281674 29.534447700334816 27.70368475937129 21.42776095801222 18-19 21.327817420151934 27.81111659420577 29.662381448089302 21.198684537552996 20-21 21.902701884943863 27.212638813346253 29.459586990897662 21.425072310812222 22-23 21.35681378570065 27.597335936402505 29.376199927238932 21.669650350657914 24-25 21.779512356774163 27.549789100968585 29.45742577110521 21.213272771152038 26-27 21.538112416454897 27.67456896939876 29.803201864422345 20.984116749723995 28-29 21.258174731681486 27.06239182157607 30.109488722427447 21.569944724315 30-31 21.605534163481867 27.1324935793762 30.025106169922307 21.236866087219628 32-33 21.102548262826414 26.988640968516375 30.22903568000202 21.679775088655195 34-35 21.180611217481676 27.154814503941914 29.845619584032047 21.81895469454436 36-37 21.310253034377304 27.246267702410393 29.93744833138516 21.506030931827148 38-39 21.428854445449012 26.984269921943945 29.93451504028874 21.652360592318303 40-41 21.62966778449757 26.954373048148373 29.964051710785565 21.451907456568485 42-43 21.832102038390467 27.070178408693867 29.775125080595487 21.322594472320176 44-45 21.612918331106076 27.38661700663855 29.60582953018684 21.39463513206854 46-47 22.118283559240837 26.905925704467602 28.873356122195364 22.102434614096193 48-49 21.891895785981607 27.05072743056181 28.962686540283332 22.094690243173247 50-51 21.646057034590324 27.36572521531152 29.165480997474972 21.822736752623182 52-53 21.61021680636551 27.663613343370997 29.328112786856902 21.398057063406586 54-55 21.66298658963119 27.795087547411757 28.68821162664208 21.853714236314975 56-57 22.084064245860365 28.3804179078672 27.981852957809387 21.553664888463047 58-59 21.399433397149696 28.463418455836138 28.911873985797108 21.22527416121706 60-61 21.891783827009736 28.795508823497627 28.085702655874588 21.22700469361805 62-63 21.21025452593621 29.68394546721927 28.058553033291368 21.04724697355315 64-65 21.37676526262137 29.368782970630136 27.95189012101313 21.302561645735363 66-67 21.16876403725377 29.256023298914478 27.932221339687587 21.642991324144166 68-69 21.418086656448622 29.12394390042271 27.972522950072676 21.485446493055992 70-71 21.500110763112822 28.87801469277194 27.85142974466851 21.770444799446725 72-73 21.56876518594167 28.643680396829257 28.043537385559464 21.74401703166961 74-75 21.542637501981297 28.66413781178403 27.953933053790404 21.839291632444272 76-77 21.539362066170774 28.696936404733176 27.90771392035733 21.855987608738722 78-79 21.561564590319826 28.349644656885875 28.011591425658906 22.077199327135393 80-81 21.534274505848405 28.54551742273872 27.788484745872616 22.13172332554026 82-83 21.750611053172882 28.427076184866774 27.84745374998424 21.974859011976104 84-85 21.778221238443958 28.19899536947818 27.79771949952182 22.225063892556044 86-87 21.510082344550348 28.347273552486545 27.813438803518554 22.329205299444553 88-89 21.801962758379727 28.063950839188166 27.73580386104002 22.398282541392085 90-91 21.629127479549457 28.033722232828207 27.94601272958458 22.39113755803776 92-93 21.628947377900086 27.999142716148995 27.862085360977733 22.509824544973185 94-95 21.622283616873364 27.898105690851914 27.902248028787447 22.57736266348727 96-97 21.514981782685354 27.902298281286864 27.934176330638405 22.64854360538937 98-99 21.614758276135948 27.55776320552697 27.95326713985459 22.8742113784825 100-101 21.496766245765322 27.832439120591744 27.79569805326374 22.87509658037919 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 298.0 1 187.0 2 68.0 3 58.0 4 53.5 5 54.0 6 63.5 7 112.0 8 194.5 9 246.5 10 202.0 11 141.5 12 140.5 13 158.0 14 174.0 15 205.5 16 232.0 17 246.5 18 270.0 19 283.0 20 310.0 21 352.5 22 414.0 23 488.5 24 553.5 25 736.0 26 992.0 27 1241.5 28 1648.0 29 2258.0 30 3067.0 31 4148.5 32 5375.0 33 6546.5 34 7793.0 35 8930.0 36 9955.0 37 10885.5 38 11329.5 39 11393.0 40 11319.0 41 10913.0 42 10579.0 43 10841.0 44 11003.0 45 10778.5 46 10559.5 47 10426.5 48 10194.0 49 9760.5 50 9188.5 51 8609.0 52 8012.5 53 7518.5 54 6974.0 55 6219.0 56 5449.5 57 4870.5 58 4388.5 59 3820.5 60 3193.5 61 2603.0 62 2141.0 63 1617.0 64 1185.0 65 895.5 66 647.5 67 497.0 68 394.5 69 309.5 70 260.0 71 212.5 72 156.0 73 110.5 74 87.0 75 71.5 76 53.5 77 36.0 78 20.0 79 17.0 80 13.0 81 8.0 82 4.5 83 1.5 84 1.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018010164937090494 2 0.0 3 0.0010806098962254295 4 0.016569351742123255 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0016209148443381445 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.403049481127148E-4 28-29 0.0045025412342726235 30-31 0.0 32-33 1.8010164937090492E-4 34-35 0.009905590715399771 36-37 0.003782134636789004 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.403049481127148E-4 60-61 0.003602032987418099 62-63 0.00954538741665796 64-65 0.002341321441821764 66-67 0.004142337935530814 68-69 0.002701524740563574 70-71 5.403049481127148E-4 72-73 0.007384167624207102 74-75 0.009005082468545247 76-77 0.002161219792450859 78-79 7.204065974836197E-4 80-81 0.008644879169803436 82-83 0.00954538741665796 84-85 0.002341321441821764 86-87 0.002521423091192669 88-89 5.403049481127148E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 1.8010164937090492E-4 98-99 1.8010164937090492E-4 100-101 9.005082468545247E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 277621.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.66668175413929 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3867954849005 52.519478895109486 2 16.977360654858305 23.65512763534519 3 4.822898454056328 10.079859951938179 4 1.5267299239859822 4.254488309554108 5 0.5830146845796106 2.0308349244298816 6 0.24008307709953575 1.0035474796108708 7 0.156705724791234 0.7642017500657844 8 0.08223346164050743 0.45831459213203624 9 0.05790510368676675 0.3630650787437775 >10 0.1548542119129063 1.615880191764031 >50 0.003633387700825657 0.17791146257752774 >100 0.004671498472490068 0.6830787658718428 >500 0.002595276929161149 1.4858499994533627 >1k 5.190553858322298E-4 0.9083609634039539 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2512 0.9048306864394263 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 950 0.3421931338047194 RNA PCR Primer, Index 27 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.29716772146199316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 792 0.2852810126035134 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 778 0.2802381664211281 RNA PCR Primer, Index 27 (96% over 25bp) TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 764 0.2751953202387427 RNA PCR Primer, Index 27 (96% over 30bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 358 0.12895278094956794 No Hit CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT 336 0.12102830837724812 RNA PCR Primer, Index 27 (96% over 28bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 5.403049481127149E-4 0.0 26-27 0.0 0.0 0.0 0.0012607115455963345 0.0 28-29 0.0 0.0 0.0 0.0027015247405635743 0.0 30-31 0.0 0.0 0.0 0.005583151130498053 0.0 32-33 0.0 0.0 0.0 0.011346403910367012 0.0 34-35 0.0 0.0 0.0 0.01963107978142864 0.0 36-37 0.0 0.0 0.0 0.03169789028927927 0.0 38-39 0.0 0.0 0.0 0.04538561564146805 0.0 40-41 0.0 0.0 0.0 0.054750901408755104 0.0 42-43 0.0 0.0 0.0 0.07005954160528202 0.0 44-45 0.0 0.0 0.0 0.08770950324363071 0.0 46-47 0.0 0.0 0.0 0.1131038358049283 0.0 48-49 0.0 0.0 0.0 0.14642264093854573 0.0 50-51 0.0 0.0 0.0 0.17307768504543963 0.0 52-53 0.0 0.0 0.0 0.19937252585359178 0.0 54-55 0.0 0.0 0.0 0.2380943804683363 0.0 56-57 0.0 0.0 0.0 0.27789684497930633 0.0 58-59 0.0 0.0 0.0 0.3236426639195162 0.0 60-61 0.0 0.0 0.0 0.37389102409399866 0.0 62-63 0.0 0.0 0.0 0.4181960298392413 0.0 64-65 0.0 0.0 0.0 0.4733071345467382 0.0 66-67 0.0 0.0 0.0 0.5675002971677214 0.0 68-69 0.0 0.0 0.0 0.6708786439066209 0.0 70-71 0.0 0.0 0.0 0.7841625813609201 0.0 72-73 0.0 0.0 0.0 0.9104138375699244 0.0 74-75 0.0 0.0 0.0 1.0359446871814453 0.0 76-77 0.0 0.0 0.0 1.1830877347174744 0.0 78-79 0.0 0.0 0.0 1.3442787109044345 0.0 80-81 0.0 0.0 0.0 1.5112329398712634 0.0 82-83 0.0 0.0 0.0 1.6979983502688918 0.0 84-85 0.0 0.0 0.0 1.8977310794212254 0.0 86-87 0.0 0.0 0.0 2.1655422320357607 0.0 88-89 0.0 0.0 0.0 2.475317068953718 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 505 0.0 64.9085 1 GTATCAA 890 0.0 37.363873 1 TCAACGC 980 0.0 33.44172 4 ATCAACG 1000 0.0 32.77289 3 TATCAAC 1015 0.0 32.756508 3 CAACGCA 1020 0.0 32.13028 5 AACGCAG 1055 0.0 31.064346 6 ACGCAGA 1170 0.0 28.011015 7 GTAACAC 85 6.397258E-4 27.939377 3 CGCAGAG 1215 0.0 26.582647 8 TATACAC 1085 0.0 25.390068 3 CTTATAC 1135 0.0 24.275936 1 ACACATC 1150 0.0 23.954979 6 TACACAT 1175 0.0 23.445297 5 CACATCT 1180 0.0 23.345953 7 TTATACA 1160 0.0 23.339014 2 ACATCTC 1170 0.0 23.139534 8 GCAGAGT 1455 0.0 22.197882 9 CATCTCC 1225 0.0 22.100615 9 CAGAGTA 1365 0.0 21.921661 10-11 >>END_MODULE