FastQCFastQC Report
Fri 10 Feb 2017
SRR2031735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490871
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15700.3198396320010756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.29111518097422745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.14565945024252808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6910.14077018198263902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.040.0696951
GTATCAA21400.037.0804561
TCAACGC25550.030.6825284
ATCAACG25750.030.4442163
TATCAAC26250.030.22942
CAACGCA26450.029.6385085
AACGCAG26450.029.626426
CGCAGAG31550.024.9929898
ACGCAGA31650.024.7588847
GTACATG26600.024.2940351
ACATGGG26100.023.482663
TACATGG27050.023.011582
TCTATAC1452.3264798E-522.9365523
GAGTACT14850.022.71358512-13
GCAGAGT34300.022.5737119
CATGGGA13850.020.582574
GTGGTAT7850.020.5802971
CATGGGG12950.020.1785984
GTACTTT16800.019.93579714-15
GTATATG2451.7445745E-719.39441
Produced by FastQC (version 0.11.5)