##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031735_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490871 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.640919915823098 33.0 31.0 34.0 30.0 34.0 2 31.967822503264607 33.0 31.0 34.0 30.0 34.0 3 32.06663665199207 34.0 31.0 34.0 30.0 34.0 4 35.49997657225625 37.0 35.0 37.0 35.0 37.0 5 35.502307123460135 37.0 35.0 37.0 35.0 37.0 6 35.53365751898156 37.0 37.0 37.0 35.0 37.0 7 35.5078788520813 37.0 36.0 37.0 35.0 37.0 8 35.497811033856145 37.0 36.0 37.0 35.0 37.0 9 37.24277661544479 39.0 38.0 39.0 35.0 39.0 10-11 37.16608538699577 39.0 38.0 39.0 35.0 39.0 12-13 37.123278824782886 39.0 38.0 39.0 35.0 39.0 14-15 38.44841923845573 41.0 38.5 41.0 34.0 41.0 16-17 38.369252817950134 41.0 38.0 41.0 34.0 41.0 18-19 38.401636071391465 41.0 38.5 41.0 34.0 41.0 20-21 38.41984452126934 41.0 39.0 41.0 34.0 41.0 22-23 38.30793731958091 40.5 39.0 41.0 34.0 41.0 24-25 38.241555316977376 40.5 38.0 41.0 34.0 41.0 26-27 38.12393887599797 40.0 38.0 41.0 34.0 41.0 28-29 38.00849510360156 40.0 38.0 41.0 34.0 41.0 30-31 37.83259552917161 40.0 38.0 41.0 33.5 41.0 32-33 37.61060237822157 40.0 38.0 41.0 33.0 41.0 34-35 37.45081090551285 40.0 38.0 41.0 33.0 41.0 36-37 37.29595963094174 40.0 37.5 41.0 32.0 41.0 38-39 37.10268889382343 40.0 37.0 41.0 31.5 41.0 40-41 37.01034691395499 40.0 37.0 41.0 31.0 41.0 42-43 36.97887836111728 40.0 37.0 41.0 31.0 41.0 44-45 36.81481387166893 40.0 37.0 41.0 30.5 41.0 46-47 36.69783201696576 40.0 37.0 41.0 30.0 41.0 48-49 36.6891281008656 40.0 37.0 41.0 30.0 41.0 50-51 36.15401398738162 39.5 36.5 40.5 29.5 41.0 52-53 36.191704134080034 39.5 37.0 40.5 30.0 41.0 54-55 36.43530275775102 40.0 37.0 41.0 30.0 41.0 56-57 36.334971917265435 40.0 37.0 41.0 29.5 41.0 58-59 36.17617357717201 40.0 36.5 41.0 29.0 41.0 60-61 35.97509427120364 40.0 36.0 41.0 28.0 41.0 62-63 35.71009796871276 39.0 35.5 41.0 28.0 41.0 64-65 35.471309162692435 39.0 35.0 41.0 28.0 41.0 66-67 35.198958585860645 39.0 35.0 41.0 27.5 41.0 68-69 34.866084979556746 38.0 35.0 40.5 26.5 41.0 70-71 34.50454396368903 37.0 35.0 40.0 26.0 41.0 72-73 34.117358735798206 37.0 35.0 39.0 26.0 41.0 74-75 33.69126002554643 36.0 34.0 39.0 26.0 41.0 76-77 33.286868647771 36.0 34.0 39.0 25.0 40.0 78-79 32.86567448474243 35.5 34.0 37.0 24.5 39.0 80-81 32.478597228192335 35.0 34.0 37.0 23.5 39.0 82-83 32.13293411099862 35.0 34.0 36.5 23.0 38.5 84-85 31.784349656019607 35.0 33.5 36.0 21.5 37.0 86-87 31.49248478724553 35.0 33.0 36.0 20.0 37.0 88-89 31.21321080283822 35.0 33.0 35.0 19.0 36.5 90-91 30.969071303865984 35.0 33.0 35.0 18.0 36.0 92-93 30.749541121801858 35.0 33.0 35.0 12.0 36.0 94-95 30.49675984118027 35.0 32.0 35.0 4.5 36.0 96-97 30.239078087725694 35.0 32.0 35.0 2.0 35.5 98-99 29.985705001925147 35.0 32.0 35.0 2.0 35.0 100-101 29.41867313408207 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.005632225798976265 1101 2 0.1505332895830982 1101 3 0.19562298436806458 1101 4 0.34147486375474756 1101 5 0.4665621860808713 1101 6 0.20810571070021666 1101 7 0.3005933475496718 1101 8 0.06411052962100428 1101 9 0.3392134594625844 1101 10-11 0.31692030232072455 1101 12-13 0.37609664084251193 1101 14-15 0.337637948960392 1101 16-17 0.4163355721063695 1101 18-19 0.5013679310079482 1101 20-21 0.42273297234307705 1101 22-23 0.3715259215182627 1101 24-25 0.23373253270345629 1101 26-27 -0.26260749943696027 1101 28-29 -0.03360878050638405 1101 30-31 0.1811054998661703 1101 32-33 0.323890438357175 1101 34-35 0.2726718691197192 1101 36-37 0.011589418091169534 1101 38-39 0.43204585949769125 1101 40-41 0.1647076191664567 1101 42-43 0.16308124807931534 1101 44-45 0.1673356157863921 1101 46-47 0.04211687599300973 1101 48-49 0.23156749238302154 1101 50-51 0.2775215432666229 1101 52-53 0.2910687653228692 1101 54-55 0.2745294842016719 1101 56-57 0.1667626503893942 1101 58-59 -0.26594612104180015 1101 60-61 -0.10805594656110173 1101 62-63 -0.007917842507033868 1101 64-65 -0.14973392434321653 1101 66-67 -0.41217868493795606 1101 68-69 -0.451519229507781 1101 70-71 -0.4968965757922774 1101 72-73 0.09330945332590801 1101 74-75 -0.032875143324652356 1101 76-77 -0.36361164890673336 1101 78-79 -0.3386867827594031 1101 80-81 -0.32276485009318634 1101 82-83 -0.35140053292994367 1101 84-85 -0.4220334402308339 1101 86-87 -0.3708673145421564 1101 88-89 -0.32759610739194756 1101 90-91 -0.6529782733260241 1101 92-93 -0.6269625458467409 1101 94-95 -0.6713740347054227 1101 96-97 -0.7401893847418854 1101 98-99 -0.5730517188273936 1101 100-101 -0.6424755628346972 1102 1 0.23982869346792768 1102 2 0.14085878951011566 1102 3 0.3579642484171117 1102 4 0.4757267977789752 1102 5 0.49779266338344996 1102 6 0.32841515457702286 1102 7 0.2664611040815643 1102 8 0.37510162581133955 1102 9 0.5551877697822505 1102 10-11 0.5634011080425196 1102 12-13 0.6738283588398843 1102 14-15 0.5303514214461558 1102 16-17 0.5208575397023409 1102 18-19 0.5595317027201077 1102 20-21 0.5106529840202043 1102 22-23 0.4672608507256868 1102 24-25 0.3792733296673987 1102 26-27 0.5524997841712818 1102 28-29 0.6057081075391579 1102 30-31 0.33973635667538815 1102 32-33 0.23140175057437062 1102 34-35 0.4785454642153226 1102 36-37 0.40956635576450395 1102 38-39 0.32192762858250035 1102 40-41 0.43098991372199436 1102 42-43 0.3431396337164472 1102 44-45 0.18939079021348704 1102 46-47 0.1739574832036439 1102 48-49 0.3795925982069903 1102 50-51 0.649610289435067 1102 52-53 0.5931239397499652 1102 54-55 0.6111197629930913 1102 56-57 0.41623863929612526 1102 58-59 0.41673086042076335 1102 60-61 0.395479303767317 1102 62-63 0.4274317414953046 1102 64-65 0.435514944435063 1102 66-67 0.37702873003795645 1102 68-69 0.5043558252287568 1102 70-71 0.2118729469051388 1102 72-73 0.3924453458963413 1102 74-75 0.34170666954938866 1102 76-77 0.5444981160910771 1102 78-79 0.32075563440597676 1102 80-81 0.2199238084968016 1102 82-83 0.50311121717953 1102 84-85 0.38528811866567736 1102 86-87 0.3096202055279065 1102 88-89 0.12719005521205062 1102 90-91 0.03135102166740822 1102 92-93 0.3080484014602227 1102 94-95 0.14176857221327666 1102 96-97 -0.06078783752250061 1102 98-99 0.061670219575756136 1102 100-101 -0.2873624406627542 1103 1 -0.1439268590963465 1103 2 0.12808721863920525 1103 3 0.16294473031238255 1103 4 0.14175991125320309 1103 5 0.05105151383438766 1103 6 0.17133344775241 1103 7 0.1504777954262977 1103 8 0.29334095815755035 1103 9 0.2169780511121715 1103 10-11 0.25769912931052374 1103 12-13 0.38932977221987386 1103 14-15 0.4338216058585971 1103 16-17 0.41252263594678595 1103 18-19 0.22712895672468392 1103 20-21 0.09076336226761583 1103 22-23 0.29563344461449503 1103 24-25 0.3951704894466417 1103 26-27 0.21911709471105922 1103 28-29 0.2457888721104311 1103 30-31 0.3261248342632257 1103 32-33 -0.01680662201951577 1103 34-35 0.07024555844066072 1103 36-37 0.05088281827628549 1103 38-39 -0.03354686327374168 1103 40-41 0.39778221416619886 1103 42-43 0.2715526098909038 1103 44-45 0.14296462249239283 1103 46-47 0.4181410879956715 1103 48-49 0.1559447317377476 1103 50-51 0.11740945486783261 1103 52-53 -0.15206653003063053 1103 54-55 -0.25836738197931197 1103 56-57 -0.383484069009171 1103 58-59 -0.25901015452068776 1103 60-61 -0.28164816964767425 1103 62-63 -0.10040780622309597 1103 64-65 -0.24232325720714698 1103 66-67 -0.4657414947567631 1103 68-69 -0.2645632755923515 1103 70-71 -0.3250929973672996 1103 72-73 -0.5971925596091125 1103 74-75 -0.2608024164648839 1103 76-77 -0.24522324613802482 1103 78-79 -0.42024046197307996 1103 80-81 -0.4273153892417909 1103 82-83 -0.4596078567200479 1103 84-85 -0.2966125409116529 1103 86-87 -0.3858408255664507 1103 88-89 -0.29372258444335486 1103 90-91 -0.3493004792075425 1103 92-93 -0.7506459046375049 1103 94-95 -0.10252541082616418 1103 96-97 -0.36861123232664994 1103 98-99 -0.5369863963887056 1103 100-101 -0.4235880430318488 1104 1 -0.28425451358538467 1104 2 -0.20167875009589054 1104 3 -0.14758748047116654 1104 4 -0.2760076008985237 1104 5 -0.48824398078240705 1104 6 -0.17925256824981517 1104 7 -0.25956727448297556 1104 8 -0.10449611492429511 1104 9 -0.3198308278207307 1104 10-11 -0.01508904211590334 1104 12-13 -0.05014739539623747 1104 14-15 -0.025926302758279007 1104 16-17 -0.21639552545814666 1104 18-19 -0.3560654869754458 1104 20-21 -0.2432470022037876 1104 22-23 0.06191300667003219 1104 24-25 -0.1552906598628354 1104 26-27 -0.3511646324150277 1104 28-29 -0.21914931083803424 1104 30-31 -0.06617630347063397 1104 32-33 -0.18349561820920712 1104 34-35 -0.22829569847925768 1104 36-37 0.18503680623977203 1104 38-39 -0.38370951151944155 1104 40-41 -0.10960796477367296 1104 42-43 -0.051779776670656474 1104 44-45 -0.1032666006116969 1104 46-47 0.056357262787607 1104 48-49 0.13106249764216926 1104 50-51 -0.03679555158986858 1104 52-53 -0.02520089838201045 1104 54-55 0.45754213522067033 1104 56-57 0.49470123745014405 1104 58-59 0.32605996911342316 1104 60-61 0.1326359369123793 1104 62-63 0.2511374860882185 1104 64-65 -0.02534192290203663 1104 66-67 0.19990515929818997 1104 68-69 0.3597353540894801 1104 70-71 0.4625934318204159 1104 72-73 0.46505589831402716 1104 74-75 0.48093521342595125 1104 76-77 0.16233252854458158 1104 78-79 0.2343718349845645 1104 80-81 0.10074210302773423 1104 82-83 0.14295968115528268 1104 84-85 0.27678281490483414 1104 86-87 0.2954061264536527 1104 88-89 0.5448564004386647 1104 90-91 0.6438238140630546 1104 92-93 0.342923728640276 1104 94-95 0.12956854466127155 1104 96-97 0.25926726648631515 1104 98-99 0.019471569623974005 1104 100-101 -0.1273431542596697 1105 1 -0.2811494955604914 1105 2 0.022577229695176726 1105 3 -0.18744222878051175 1105 4 -0.12181451087931805 1105 5 0.0969466620615762 1105 6 -0.05302569142430258 1105 7 0.14928992100159633 1105 8 -0.03137557180927786 1105 9 0.024110439973917153 1105 10-11 0.0787877570841573 1105 12-13 0.007813818192161648 1105 14-15 0.1262932588552772 1105 16-17 0.052159588611793595 1105 18-19 -0.02046829727989774 1105 20-21 0.19233176789330741 1105 22-23 -0.16270610234767702 1105 24-25 -0.19283506425196606 1105 26-27 -0.16693180869058466 1105 28-29 -0.10022712048463944 1105 30-31 0.03581038179105178 1105 32-33 0.24238654898811518 1105 34-35 0.0746988019549164 1105 36-37 -0.0640093216974904 1105 38-39 0.0588476550200383 1105 40-41 0.2425563380445297 1105 42-43 0.23683428011433705 1105 44-45 -0.03228799365961521 1105 46-47 0.14440031202055792 1105 48-49 -0.016415333055405767 1105 50-51 -0.18960980309632447 1105 52-53 0.001557515427421663 1105 54-55 -0.05691724956474076 1105 56-57 -0.1185135087541056 1105 58-59 -0.21060555729238928 1105 60-61 -0.10641098043625163 1105 62-63 0.0627655154543234 1105 64-65 -0.006864434282711329 1105 66-67 0.0684497478832995 1105 68-69 -0.02155296525372563 1105 70-71 -0.3239102652561243 1105 72-73 -0.6692466567474114 1105 74-75 -0.22906662853389292 1105 76-77 0.1066395569370755 1105 78-79 -0.091286665660121 1105 80-81 0.05833755601828017 1105 82-83 0.05626389398059217 1105 84-85 -0.059245919668754254 1105 86-87 0.3170491546356402 1105 88-89 0.2034491431168739 1105 90-91 0.03642702959866995 1105 92-93 0.19887457462611735 1105 94-95 0.3157143752535916 1105 96-97 0.10580832903400861 1105 98-99 -0.0016741293997881712 1105 100-101 0.1593394760589888 1106 1 0.5125661520877429 1106 2 0.5155875901212674 1106 3 0.4551376632375863 1106 4 0.47826817796741494 1106 5 0.4161985712011287 1106 6 0.4283693180605965 1106 7 0.48537630652617736 1106 8 0.5827155804841198 1106 9 0.7430461858759543 1106 10-11 0.6120311054960439 1106 12-13 0.5660437904670843 1106 14-15 0.5442780830198757 1106 16-17 0.6541246647341694 1106 18-19 0.6801810533694521 1106 20-21 0.6060362282269764 1106 22-23 0.6971411664883504 1106 24-25 0.756355071455026 1106 26-27 0.6645191373671011 1106 28-29 0.6360951714556293 1106 30-31 0.5615825496980591 1106 32-33 0.6886923031590371 1106 34-35 0.6707710817350616 1106 36-37 0.6759707356982929 1106 38-39 0.6651514635710356 1106 40-41 0.38671947827509 1106 42-43 0.5711355593594334 1106 44-45 0.6031417451409098 1106 46-47 0.6160939741636682 1106 48-49 0.752398811601438 1106 50-51 0.5933052219535355 1106 52-53 0.7030653708678685 1106 54-55 0.8266252379103349 1106 56-57 0.7643236914988236 1106 58-59 1.0227812805888306 1106 60-61 0.7167321685495907 1106 62-63 0.8416385151138357 1106 64-65 0.9256906510813607 1106 66-67 1.022185338135749 1106 68-69 0.9677120768203054 1106 70-71 0.9614756970961267 1106 72-73 0.6718545641290845 1106 74-75 0.8873471075427588 1106 76-77 0.8276863004557313 1106 78-79 0.7338750639959954 1106 80-81 0.5830712492913008 1106 82-83 0.6313210465658301 1106 84-85 0.9510074973262306 1106 86-87 0.8268623751230102 1106 88-89 0.6593443714840141 1106 90-91 0.7315216353674359 1106 92-93 0.8415930920637358 1106 94-95 0.8844652872629339 1106 96-97 0.9560418034247853 1106 98-99 0.9743363769477966 1106 100-101 1.1765387561796175 1107 1 0.44231686114879665 1107 2 0.5401285663863824 1107 3 0.5226701307700523 1107 4 0.5149154387526096 1107 5 0.3916128121326068 1107 6 0.47354023909358745 1107 7 0.5444597518165253 1107 8 0.5568460477180395 1107 9 0.644464307983732 1107 10-11 0.7770184170350802 1107 12-13 0.8170737949453581 1107 14-15 0.9955170739140442 1107 16-17 0.8702016911560193 1107 18-19 0.7484972199614788 1107 20-21 0.6518564999093215 1107 22-23 0.849059363234133 1107 24-25 0.7958385764557789 1107 26-27 0.810376728052276 1107 28-29 0.7538216978028487 1107 30-31 0.8474983580276643 1107 32-33 1.0720778830963482 1107 34-35 1.1806009070821304 1107 36-37 1.1477948885569305 1107 38-39 1.101156837507446 1107 40-41 0.896101475588118 1107 42-43 0.9301443666441003 1107 44-45 1.0656585386921833 1107 46-47 1.2703334128858543 1107 48-49 1.328760955204963 1107 50-51 1.2358712765885471 1107 52-53 1.2664031824815325 1107 54-55 1.3251697967997131 1107 56-57 1.2783481727646802 1107 58-59 1.2303346467628913 1107 60-61 1.3146273767896304 1107 62-63 1.4184932695548014 1107 64-65 1.4586657219874652 1107 66-67 1.1697670667519517 1107 68-69 1.1704139726256528 1107 70-71 1.1493171659720787 1107 72-73 1.1879017353486745 1107 74-75 0.9089548101849445 1107 76-77 1.1469727611215035 1107 78-79 1.250802963682517 1107 80-81 1.468307851769282 1107 82-83 1.5791490806007644 1107 84-85 1.2975666852647336 1107 86-87 1.4076729438646218 1107 88-89 1.3679202783357844 1107 90-91 1.3442921981379925 1107 92-93 1.7550801796887576 1107 94-95 1.6094764829637853 1107 96-97 1.6571837649115722 1107 98-99 1.7304193744101042 1107 100-101 1.7692839420282525 1108 1 0.2499429019905861 1108 2 0.23038844688454319 1108 3 0.06880791782641893 1108 4 0.03575627094611633 1108 5 0.24932404002596797 1108 6 0.2931308613190069 1108 7 0.32370290685695835 1108 8 0.34528460505732284 1108 9 0.2919530896889171 1108 10-11 0.22329058366575794 1108 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539.0 5 485.0 6 514.0 7 546.0 8 616.0 9 715.0 10 772.0 11 942.0 12 1242.0 13 1549.0 14 1703.0 15 2029.0 16 2471.0 17 3492.0 18 3340.0 19 1914.0 20 1697.0 21 1696.0 22 1794.0 23 1871.0 24 2168.0 25 2488.0 26 2909.0 27 3468.0 28 4141.0 29 5006.0 30 6019.0 31 7542.0 32 9825.0 33 13461.0 34 18253.0 35 27991.0 36 46031.0 37 95301.0 38 158493.0 39 51254.0 40 58.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.55676672781477 21.368710808930206 14.794915797227725 22.2796066660273 2 15.660174838588558 22.691265416731284 40.252667063903424 21.395892680776736 3 17.40325460388716 28.65173697903382 31.42621027393766 22.518798143141357 4 12.558284749954595 19.807530236648837 39.655056310465646 27.97912870293092 5 12.760092549896553 38.65998522792656 35.426655839253726 13.153266382923157 6 27.52249973481727 38.449660158130506 20.236094225544033 13.791745881508197 7 24.482905642902463 34.464933056706464 24.78340075603183 16.26876054435924 8 23.98213120104439 34.43007506527415 23.018521540469976 18.56927219321149 9 25.901494893619624 16.747948575169957 22.731806416477486 34.61875011473293 10-11 22.74202166712894 27.47752524840515 30.400976489207228 19.379476595258684 12-13 24.20269648665271 26.46907934124866 29.487863362234705 19.84036080986393 14-15 22.299174202894115 27.86491407935609 27.85644698117346 21.979464736576336 16-17 20.347859089318455 30.087627924629167 28.95639212501543 20.608120861036948 18-19 20.17506988512314 29.142810504193108 30.837703278989576 19.844416331694177 20-21 21.954247532580144 28.305016449465707 30.20366733824692 19.537068679707225 22-23 21.496834290645474 28.394785938477014 29.904554398484624 20.203825372392892 24-25 20.806276752741184 28.56287614468841 30.592846353978093 20.03800074859231 26-27 20.712336200003264 28.723441146232805 30.399994288418547 20.164228365345387 28-29 20.70529604421003 28.43378950256576 30.62786933235143 20.233045120872777 30-31 21.231016508536772 28.413917878519936 30.31402629005133 20.04103932289197 32-33 20.918805467225003 28.41043757789123 30.22852853649134 20.44222841839243 34-35 20.961881738527293 28.794842763446454 30.155757520119952 20.0875179779063 36-37 21.009623133057104 28.510036252506772 29.90250380987937 20.57783680455676 38-39 20.66899339920529 28.675312701430304 30.32816311877351 20.327530780590887 40-41 21.113612186845657 28.41900849289165 30.14828647981013 20.31909284045256 42-43 21.00690442923725 28.54996787451684 29.703731655329264 20.739396040916645 44-45 21.196721562307147 28.690001989095677 29.54164095292984 20.571635495667344 46-47 21.2968270904013 28.625140509322065 29.27653254614112 20.801499854135514 48-49 21.117074618886967 28.691999049194006 29.34307671433279 20.84784961758624 50-51 21.11015113943651 28.85827859964727 29.182141534732896 20.849428726183326 52-53 21.24382346192091 28.973560082608806 28.984166645419545 20.798449810050737 54-55 21.366772655980714 28.89286377143836 28.75855851813478 20.981805054446145 56-57 21.358293417326944 29.07510465201674 28.629364580698336 20.937237349957975 58-59 21.087404385517594 28.86721060683325 29.166649668536458 20.878735339112698 60-61 21.39557131143194 29.04674681494234 28.76016301620779 20.79751885741793 62-63 21.202135634188505 29.03544602016308 28.866961412741393 20.895456932907024 64-65 21.38929693556943 28.791059697229365 28.80993185620435 21.00971151099686 66-67 21.127827985067015 29.21428425712479 28.61329280483078 21.044594952977416 68-69 21.175238108838972 29.023725708410137 28.722602310040813 21.078433872710082 70-71 21.1985035534486 28.697361227836844 28.974375188687713 21.129760030026844 72-73 21.19774306334681 28.672111818727025 28.83907698960879 21.291068128317374 74-75 21.17949507608938 28.923653636514747 28.371555633510532 21.525295653885337 76-77 20.95496506349773 28.97057173458459 28.852144642219617 21.222318559698067 78-79 21.13386498236337 28.822476962215383 28.680486277293937 21.363171778127317 80-81 20.947217561164692 29.07466249126583 28.539544752102575 21.438575195466903 82-83 21.04781549535433 29.067459103020806 28.556680418008135 21.328044983616724 84-85 21.031968249013904 28.8507811774834 28.472866105863144 21.644384467639554 86-87 20.840929006511452 28.909089610751295 28.771984947050466 21.477996435686794 88-89 21.13802895050345 28.763239061964523 28.6865381790884 21.412193808443625 90-91 20.922465825248462 28.831953354435093 28.709443462347846 21.536137357968602 92-93 20.887464215975335 28.968277449382057 28.802492588141433 21.341765746501174 94-95 20.917449907439046 28.630046866951915 28.81557675929562 21.636926466313422 96-97 20.972959302301867 28.755879077622183 28.766892787651592 21.504268832424362 98-99 20.90199293874329 28.62582886140805 28.79124420485395 21.680933994994707 100-101 20.80696670713914 28.79164158393617 28.727871930128867 21.673519778795825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1388.0 1 788.5 2 193.0 3 173.5 4 125.0 5 126.0 6 176.5 7 276.0 8 403.5 9 445.0 10 360.5 11 271.5 12 274.5 13 310.5 14 351.0 15 386.5 16 384.0 17 401.5 18 461.5 19 494.0 20 569.0 21 646.5 22 730.0 23 828.0 24 971.0 25 1226.5 26 1613.5 27 2184.5 28 2835.5 29 3863.0 30 5501.5 31 7489.0 32 10257.0 33 13726.0 34 16446.5 35 18714.0 36 21491.0 37 23509.0 38 23655.0 39 23165.5 40 22635.5 41 21703.5 42 20975.0 43 20635.0 44 20378.0 45 19990.0 46 19219.5 47 18313.0 48 17457.0 49 16198.5 50 14830.0 51 13727.0 52 12423.0 53 10875.5 54 9472.5 55 8063.5 56 6969.5 57 5944.0 58 4809.5 59 4111.5 60 3427.5 61 2704.5 62 2031.0 63 1448.5 64 1135.0 65 941.0 66 740.0 67 584.5 68 457.5 69 365.0 70 304.0 71 254.5 72 227.0 73 183.5 74 161.0 75 139.0 76 111.5 77 90.0 78 71.0 79 56.0 80 41.5 81 27.0 82 21.0 83 21.0 84 19.0 85 11.5 86 6.5 87 9.0 88 9.0 89 5.5 90 5.5 91 6.0 92 3.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16379048670628332 2 0.166846279368714 3 0.16216073061965364 4 0.1670499988795427 5 0.15421566969733394 6 0.13099164546286093 7 0.1385292673635232 8 0.12854701133291638 9 0.12263914551888379 10-11 0.11061969437998985 12-13 0.13048234668578915 14-15 0.15044685874700278 16-17 0.1604291147776096 18-19 0.15910493795722297 20-21 0.1494282611928592 22-23 0.14189063929219692 24-25 0.1257967979367288 26-27 0.13017676741954606 28-29 0.1355753344565069 30-31 0.12416704185009911 32-33 0.1230465845405412 34-35 0.13975158442849547 36-37 0.14219621855844 38-39 0.12773213328960156 40-41 0.129871188153303 42-43 0.12345402356219862 44-45 0.14229807831385435 46-47 0.14056646247181032 48-49 0.1549286879852344 50-51 0.1414832002705395 52-53 0.1239633223392704 54-55 0.11612012117236505 56-57 0.13751066980937965 58-59 0.12650981622462928 60-61 0.1248800601379996 62-63 0.1260005174475575 64-65 0.14891896241578745 66-67 0.13873298687435193 68-69 0.14392783440048404 70-71 0.13099164546286093 72-73 0.13221396252783318 74-75 0.11398106630866357 76-77 0.14178877953678257 78-79 0.14219621855844 80-81 0.14229807831385435 82-83 0.1501412794807597 84-85 0.13883484662976628 86-87 0.1496319807036879 88-89 0.13343627959280543 90-91 0.14392783440048404 92-93 0.15849377942473683 94-95 0.13313070032656238 96-97 0.11673127970485118 98-99 0.12019451138893926 100-101 0.16827231594451494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 490871.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.66099865880112 #Duplication Level Percentage of deduplicated Percentage of total 1 68.20024373544756 37.96093675088656 2 17.7054401131932 19.710049567878606 3 6.749033687063885 11.269738650115995 4 2.9722456104196375 6.617526357407799 5 1.5130241018566988 4.210821625208974 6 0.825998935547034 2.7585555386192766 7 0.5084973616371146 1.9812429674861138 8 0.3227080750399547 1.4369802985586568 9 0.20809982559659404 1.042473970208589 >10 0.9269388723119658 9.064470178315343 >50 0.05414208299561133 1.9938977506201687 >100 0.01215434495656506 1.0502506721102924 >500 7.366269670645489E-4 0.28823979015949064 >1k 7.366269670645489E-4 0.6148158824241198 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1570 0.3198396320010756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.29111518097422745 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 715 0.14565945024252808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 691 0.14077018198263902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.037195108287106E-4 0.0 0.0 0.0 0.0 6 2.037195108287106E-4 0.0 0.0 0.0 0.0 7 2.037195108287106E-4 0.0 0.0 0.0 0.0 8 2.037195108287106E-4 0.0 0.0 0.0 0.0 9 2.037195108287106E-4 0.0 0.0 6.111585324861318E-4 0.0 10-11 2.037195108287106E-4 0.0 0.0 6.111585324861318E-4 0.0 12-13 2.037195108287106E-4 0.0 0.0 0.001018597554143553 0.0 14-15 2.037195108287106E-4 0.0 0.0 0.0011204573095579084 0.0 16-17 2.037195108287106E-4 0.0 0.0 0.0012223170649722636 0.0 18-19 2.037195108287106E-4 0.0 0.0 0.0012223170649722636 0.0 20-21 2.037195108287106E-4 1.018597554143553E-4 0.0 0.0018334755974583955 0.0 22-23 2.037195108287106E-4 2.037195108287106E-4 0.0 0.002240914619115817 0.0 24-25 2.037195108287106E-4 2.037195108287106E-4 0.0 0.0028520731516019486 0.0 26-27 2.037195108287106E-4 2.037195108287106E-4 0.0 0.003361371928673725 0.0 28-29 2.037195108287106E-4 2.037195108287106E-4 0.0 0.0055004267923751855 0.0 30-31 2.037195108287106E-4 2.037195108287106E-4 0.0 0.010898993829336016 0.0 32-33 2.037195108287106E-4 2.037195108287106E-4 0.0 0.01843661572999831 0.0 34-35 2.037195108287106E-4 2.037195108287106E-4 0.0 0.030150487602649167 0.0 36-37 2.037195108287106E-4 2.037195108287106E-4 0.0 0.04369783507275842 0.0 38-39 2.037195108287106E-4 2.037195108287106E-4 0.0 0.05704146303203897 0.0 40-41 2.037195108287106E-4 2.037195108287106E-4 0.0 0.06916277392634726 0.0 42-43 2.037195108287106E-4 2.037195108287106E-4 0.0 0.09065518231877621 0.0 44-45 2.037195108287106E-4 2.037195108287106E-4 0.0 0.11642570043860812 0.0 46-47 2.037195108287106E-4 2.037195108287106E-4 0.0 0.14504829171004194 0.0 48-49 2.037195108287106E-4 2.037195108287106E-4 0.0 0.18762566947324244 0.0 50-51 2.037195108287106E-4 2.037195108287106E-4 0.0 0.22103566924915102 0.0 52-53 2.037195108287106E-4 2.037195108287106E-4 0.0 0.2599460958174347 0.0 54-55 2.037195108287106E-4 2.037195108287106E-4 0.0 0.3128113088774851 0.0 56-57 2.037195108287106E-4 2.037195108287106E-4 0.0 0.36628768047002164 0.0 58-59 2.037195108287106E-4 2.037195108287106E-4 0.0 0.4187454545084146 0.0 60-61 2.037195108287106E-4 2.037195108287106E-4 0.0 0.4740553016984096 0.0 62-63 2.037195108287106E-4 2.037195108287106E-4 0.0 0.5198921916348694 0.0 64-65 2.037195108287106E-4 2.037195108287106E-4 0.0 0.5809061851280684 0.0 66-67 2.037195108287106E-4 2.037195108287106E-4 0.0 0.6805250259233078 0.0 68-69 2.037195108287106E-4 2.037195108287106E-4 0.0 0.8029604519313628 0.0 70-71 2.037195108287106E-4 2.037195108287106E-4 0.0 0.93059072546555 0.0 72-73 2.037195108287106E-4 2.037195108287106E-4 0.0 1.0663697794328857 0.0 74-75 2.037195108287106E-4 2.037195108287106E-4 0.0 1.2192612723098328 0.0 76-77 2.037195108287106E-4 2.037195108287106E-4 0.0 1.3837647773040167 0.0 78-79 2.037195108287106E-4 2.037195108287106E-4 0.0 1.5739369406626182 0.0 80-81 2.037195108287106E-4 2.037195108287106E-4 0.0 1.765127701575363 0.0 82-83 2.037195108287106E-4 2.037195108287106E-4 0.0 1.9770978525926364 0.0 84-85 2.037195108287106E-4 2.037195108287106E-4 0.0 2.2078101986061514 0.0 86-87 2.037195108287106E-4 2.037195108287106E-4 0.0 2.487618946729385 0.0 88-89 2.037195108287106E-4 2.037195108287106E-4 0.0 2.8043009263126155 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1340 0.0 40.069695 1 GTATCAA 2140 0.0 37.080456 1 TCAACGC 2555 0.0 30.682528 4 ATCAACG 2575 0.0 30.444216 3 TATCAAC 2625 0.0 30.2294 2 CAACGCA 2645 0.0 29.638508 5 AACGCAG 2645 0.0 29.62642 6 CGCAGAG 3155 0.0 24.992989 8 ACGCAGA 3165 0.0 24.758884 7 GTACATG 2660 0.0 24.294035 1 ACATGGG 2610 0.0 23.48266 3 TACATGG 2705 0.0 23.01158 2 TCTATAC 145 2.3264798E-5 22.936552 3 GAGTACT 1485 0.0 22.713585 12-13 GCAGAGT 3430 0.0 22.573711 9 CATGGGA 1385 0.0 20.58257 4 GTGGTAT 785 0.0 20.580297 1 CATGGGG 1295 0.0 20.178598 4 GTACTTT 1680 0.0 19.935797 14-15 GTATATG 245 1.7445745E-7 19.3944 1 >>END_MODULE