FastQCFastQC Report
Fri 10 Feb 2017
SRR2031735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490871
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT28090.572248105917848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.38808566812869366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.34163761965974765No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT11380.23183280332307266RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT8690.1770322549101495RNA PCR Primer, Index 27 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.16358676719545462No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG7130.14525201122087067RNA PCR Primer, Index 27 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6590.13425115763612028No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.11510152361822149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12650.050.6984941
GTATCAA21700.040.7197461
TCAACGC26100.033.12034
ATCAACG26450.032.682033
TATCAAC27050.032.483872
CAACGCA26650.032.2585375
AACGCAG27400.031.3755536
CGCAGAG31600.027.6562988
ACGCAGA31400.027.3786647
GCAGAGT34850.024.9408729
GAGTACT18450.023.9426412-13
GTACTTT19800.022.07141914-15
GTACATG22350.021.680761
CAGAGTA33550.021.44783410-11
ACATGGG21900.021.0373463
GTATAGG2308.981442E-820.6549421
TACATGG23000.020.4442252
AGAGTAC30950.020.41053210-11
CTCGTAT7700.020.04730438-39
TGCCGTC7950.019.71560744-45
Produced by FastQC (version 0.11.5)