##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	490871
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.757952700403976	34.0	31.0	34.0	31.0	34.0
2	32.9202784438274	34.0	31.0	34.0	31.0	34.0
3	32.97466747882845	34.0	31.0	34.0	31.0	34.0
4	36.37575248894312	37.0	37.0	37.0	35.0	37.0
5	36.35019587630966	37.0	37.0	37.0	35.0	37.0
6	36.38424351815446	37.0	37.0	37.0	35.0	37.0
7	36.32934722157145	37.0	37.0	37.0	35.0	37.0
8	36.321811636865895	37.0	37.0	37.0	35.0	37.0
9	38.16862271350314	39.0	39.0	39.0	37.0	39.0
10-11	38.14154533472134	39.0	39.0	39.0	37.0	39.0
12-13	38.098061405134956	39.0	39.0	39.0	37.0	39.0
14-15	39.56427146847135	41.0	39.0	41.0	37.0	41.0
16-17	39.536262072927514	41.0	39.0	41.0	37.0	41.0
18-19	39.57375868609064	41.0	39.5	41.0	37.0	41.0
20-21	39.57491377571704	41.0	40.0	41.0	37.0	41.0
22-23	39.48100213701767	41.0	39.0	41.0	36.5	41.0
24-25	39.4078994277519	41.0	39.0	41.0	36.0	41.0
26-27	39.2752871935804	41.0	39.0	41.0	36.0	41.0
28-29	39.179566525624864	41.0	39.0	41.0	36.0	41.0
30-31	38.99547437106693	40.0	38.5	41.0	35.0	41.0
32-33	38.78584903161931	40.0	38.0	41.0	35.0	41.0
34-35	38.648264004188476	40.0	38.0	41.0	35.0	41.0
36-37	38.53374817416389	40.0	38.0	41.0	35.0	41.0
38-39	38.59384237406569	40.0	38.0	41.0	35.0	41.0
40-41	38.525900898606764	40.0	38.0	41.0	35.0	41.0
42-43	38.41512841459365	40.0	38.0	41.0	35.0	41.0
44-45	38.22034302291233	40.0	38.0	41.0	34.0	41.0
46-47	37.97460534437765	40.0	38.0	41.0	33.5	41.0
48-49	37.718199893658415	40.0	38.0	41.0	33.0	41.0
50-51	37.54394841007108	40.0	38.0	41.0	33.0	41.0
52-53	37.62988850431172	40.0	38.0	41.0	33.0	41.0
54-55	37.58094896622534	40.0	38.0	41.0	33.0	41.0
56-57	37.40081915615304	40.0	37.5	41.0	33.0	41.0
58-59	37.22511107806328	40.0	37.0	41.0	33.0	41.0
60-61	36.97630843948818	40.0	37.0	41.0	32.0	41.0
62-63	36.70977405469054	40.0	36.0	41.0	31.5	41.0
64-65	36.39043047969833	39.0	36.0	41.0	31.0	41.0
66-67	36.00450933137219	39.0	35.5	41.0	30.5	41.0
68-69	35.56236057945978	38.5	35.0	40.5	30.0	41.0
70-71	35.20082465657983	37.5	35.0	40.0	29.0	41.0
72-73	34.80649702263935	37.0	35.0	39.5	29.0	41.0
74-75	34.38885980226984	37.0	35.0	39.0	29.0	41.0
76-77	33.422453149605495	36.0	33.5	38.0	27.5	39.0
78-79	33.57602200985595	36.0	34.0	37.5	28.0	39.0
80-81	33.29694563337415	35.0	34.0	37.0	28.0	39.0
82-83	32.97390047487018	35.0	34.0	37.0	28.0	39.0
84-85	32.63107313326719	35.0	34.0	36.0	27.0	37.0
86-87	32.35615670919651	35.0	34.0	36.0	26.0	37.0
88-89	32.11564952910235	35.0	34.0	35.5	26.0	37.0
90-91	31.8977613262955	35.0	34.0	35.0	26.0	36.0
92-93	31.716026206477874	35.0	34.0	35.0	25.5	36.0
94-95	31.53715843877516	35.0	34.0	35.0	25.0	36.0
96-97	31.367970403629467	35.0	34.0	35.0	24.5	36.0
98-99	31.16845057051649	35.0	33.0	35.0	23.5	35.0
100-101	30.51939002304068	34.5	32.0	35.0	11.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12217049261086999
1101	2	0.11321048713526238
1101	3	0.14361651242678164
1101	4	0.07770779455597676
1101	5	0.1519896945415482
1101	6	0.01766280061782055
1101	7	0.07107390303005445
1101	8	0.04305359366829009
1101	9	0.05368612118499527
1101	10-11	0.06967930788994892
1101	12-13	0.15832816555583662
1101	14-15	0.1182766789272165
1101	16-17	0.03394531357296415
1101	18-19	0.04789517817388145
1101	20-21	0.048381624345999796
1101	22-23	-0.0977559056290076
1101	24-25	-0.01579935815865241
1101	26-27	0.1616824212613892
1101	28-29	0.1078825644519057
1101	30-31	0.2518797833121553
1101	32-33	0.25857937205477555
1101	34-35	-0.05794207579280197
1101	36-37	-0.01327356583889383
1101	38-39	0.10943272246260705
1101	40-41	-0.008649740613357437
1101	42-43	0.3304853595047561
1101	44-45	0.2437310170089475
1101	46-47	0.3422926718134107
1101	48-49	0.06952371180117467
1101	50-51	0.12198682418971885
1101	52-53	0.3337727339368399
1101	54-55	0.21465267262296805
1101	56-57	-0.06280852014810989
1101	58-59	0.042075859266979876
1101	60-61	0.059518614045082074
1101	62-63	-0.0020039316389883766
1101	64-65	0.14619090494853282
1101	66-67	-0.40815277445043563
1101	68-69	-0.22722847552113024
1101	70-71	-0.007988557733483503
1101	72-73	-0.05600110862890517
1101	74-75	0.051256900461496
1101	76-77	-0.12486156859521458
1101	78-79	-0.08939019608388321
1101	80-81	-0.15346504324227084
1101	82-83	-0.13087623278627092
1101	84-85	-0.11272096496583828
1101	86-87	-0.10118972546066374
1101	88-89	-0.1971801462249516
1101	90-91	-0.3033625401430058
1101	92-93	-0.37240899674134553
1101	94-95	-0.27993470991778935
1101	96-97	-0.2914066506127071
1101	98-99	-0.32599391445932824
1101	100-101	-0.2908225863416263
1102	1	0.2244212589223622
1102	2	0.08985039371823689
1102	3	0.10386757066645913
1102	4	0.14570954612509013
1102	5	0.13703713287171837
1102	6	0.14012667450484884
1102	7	0.23824365927001878
1102	8	0.14181873744291806
1102	9	0.11245710352334015
1102	10-11	0.27112435240899657
1102	12-13	0.19157878590322497
1102	14-15	0.3261616592220662
1102	16-17	0.19323592808179058
1102	18-19	0.09173987237910097
1102	20-21	0.20118851385118575
1102	22-23	0.18470796825801727
1102	24-25	0.29222574766530585
1102	26-27	0.3589674891346917
1102	28-29	0.49967646315281655
1102	30-31	0.40774024163940936
1102	32-33	0.33412746722336806
1102	34-35	0.3101180614134549
1102	36-37	0.36931291788611276
1102	38-39	0.20944439959003347
1102	40-41	0.32961620596378793
1102	42-43	0.3504532474043245
1102	44-45	0.49233122135867546
1102	46-47	0.5630487658142869
1102	48-49	0.5759286161946946
1102	50-51	0.6288675715258734
1102	52-53	0.7219029339076428
1102	54-55	0.5963721296365421
1102	56-57	0.6792729278156884
1102	58-59	0.725529369703402
1102	60-61	1.0511957414717301
1102	62-63	0.9297082271699395
1102	64-65	0.9970973419650306
1102	66-67	0.9399920219296618
1102	68-69	0.9700186416880427
1102	70-71	0.7929222582057918
1102	72-73	1.1797309012966508
1102	74-75	1.0344243212759707
1102	76-77	1.131702436659502
1102	78-79	1.000021568622003
1102	80-81	0.794720623083812
1102	82-83	0.8295149509825208
1102	84-85	0.855663212526494
1102	86-87	0.8279972545422609
1102	88-89	0.6849626066578409
1102	90-91	0.5428584494935507
1102	92-93	0.8023567308896595
1102	94-95	0.7843537151296545
1102	96-97	0.5799697907827088
1102	98-99	0.6209656534869588
1102	100-101	0.4575323983321482
1103	1	0.2612929778616504
1103	2	0.23349826018748132
1103	3	0.19516922634016964
1103	4	0.13810152351101124
1103	5	0.08437324809584368
1103	6	0.13245404023374618
1103	7	0.2133250968793874
1103	8	0.14053188860727772
1103	9	0.2670458772479307
1103	10-11	0.2331784646717523
1103	12-13	0.2222424014638662
1103	14-15	0.3335812311698376
1103	16-17	0.4326086565782248
1103	18-19	0.3775764587298909
1103	20-21	0.3053048148338178
1103	22-23	0.2927790410807418
1103	24-25	0.21063737776356817
1103	26-27	0.35803869656503196
1103	28-29	0.2399214490190218
1103	30-31	0.3379192697723923
1103	32-33	0.22161972728528667
1103	34-35	0.3214493307632935
1103	36-37	0.3336553596683203
1103	38-39	0.2050400383031885
1103	40-41	0.10316042456655339
1103	42-43	0.11304579014224458
1103	44-45	0.3021118109400476
1103	46-47	0.34894188736497256
1103	48-49	0.46379777758922813
1103	50-51	0.33016518897101577
1103	52-53	0.41266615910619464
1103	54-55	0.5682875960519382
1103	56-57	0.35762802271406
1103	58-59	0.4223445534428052
1103	60-61	0.44944315027506576
1103	62-63	0.134696785522344
1103	64-65	0.41710811057453157
1103	66-67	0.3448836627965264
1103	68-69	0.4664703848567058
1103	70-71	0.3887413455661388
1103	72-73	0.42000550083898247
1103	74-75	0.6183225579161657
1103	76-77	0.6480061114474935
1103	78-79	0.8236532821769842
1103	80-81	0.6201237729020903
1103	82-83	0.7109848661797216
1103	84-85	0.5470617856857416
1103	86-87	0.4122023965532904
1103	88-89	0.6722771846629527
1103	90-91	0.5607828000117792
1103	92-93	0.48242403469905426
1103	94-95	0.6427788059897921
1103	96-97	0.6317668024935728
1103	98-99	0.717751223550227
1103	100-101	0.27811121109294845
1104	1	0.17762693463527057
1104	2	0.08507838655472
1104	3	0.10577967977239666
1104	4	0.10811208090949975
1104	5	0.1370316224708361
1104	6	0.11422779036102781
1104	7	0.1793484946467956
1104	8	0.24269213598266504
1104	9	0.12516408795645617
1104	10-11	0.2223022005974542
1104	12-13	0.241695881921963
1104	14-15	0.2762952864434496
1104	16-17	0.16751613196662163
1104	18-19	0.20333651103456418
1104	20-21	0.20333481499459083
1104	22-23	0.17679503259194718
1104	24-25	0.039433352018527046
1104	26-27	0.07368025948873225
1104	28-29	-0.04228799476148737
1104	30-31	0.11655688305006606
1104	32-33	-0.00493025422586868
1104	34-35	0.10888924201683636
1104	36-37	0.04648608223381956
1104	38-39	-0.25695868623446216
1104	40-41	-0.16627027977805398
1104	42-43	-0.1320787818007929
1104	44-45	-0.011035633580021909
1104	46-47	0.1365988415822983
1104	48-49	0.14247772764255018
1104	50-51	0.0976291089277197
1104	52-53	0.17110805858045808
1104	54-55	0.19618753120700916
1104	56-57	-0.15603496583356957
1104	58-59	-0.08106382495150655
1104	60-61	0.038133336181751076
1104	62-63	-0.1085119133452821
1104	64-65	-0.24605460733928197
1104	66-67	-0.47552802215354717
1104	68-69	-0.12621499930934021
1104	70-71	-0.2094692557768454
1104	72-73	-0.600128583480867
1104	74-75	-0.3892042777046072
1104	76-77	-0.3112621590148379
1104	78-79	-0.22990541856631808
1104	80-81	-0.2643343431649825
1104	82-83	-0.17079638666729124
1104	84-85	-0.3398320347527459
1104	86-87	-0.36841271928333796
1104	88-89	-0.151315117546595
1104	90-91	-0.28585624950080657
1104	92-93	-0.17200337380796427
1104	94-95	0.06855815100235674
1104	96-97	0.10129504219474939
1104	98-99	0.22978505009806227
1104	100-101	0.24699100144552233
1105	1	0.0697880798080206
1105	2	-0.02560234917667259
1105	3	-0.08003196667657164
1105	4	-0.03528186167397962
1105	5	-0.015824730974941303
1105	6	-0.027487337392045674
1105	7	0.016935000975244918
1105	8	-0.13069943836672593
1105	9	-0.035275877309445036
1105	10-11	0.09141516536339367
1105	12-13	0.12528665107176806
1105	14-15	0.28587084626767023
1105	16-17	0.22059878333624994
1105	18-19	0.14571211296073017
1105	20-21	0.17122156077781625
1105	22-23	0.15934114737236627
1105	24-25	0.13397062539167592
1105	26-27	0.029357442868992223
1105	28-29	0.03296132766042348
1105	30-31	0.10245273337767458
1105	32-33	0.038000567025086696
1105	34-35	0.3477651202369785
1105	36-37	0.1693790117393874
1105	38-39	0.288003553588716
1105	40-41	0.05448071356457973
1105	42-43	0.360763888514704
1105	44-45	0.45665375936264496
1105	46-47	0.48115848161071995
1105	48-49	0.5201189664921131
1105	50-51	0.7077704135615619
1105	52-53	0.6558487369479593
1105	54-55	0.5575221626834761
1105	56-57	0.28429606066433877
1105	58-59	0.4836126479585303
1105	60-61	0.4168665940824354
1105	62-63	0.294427328293537
1105	64-65	0.550448300325904
1105	66-67	0.7573614865694438
1105	68-69	0.5668329179603475
1105	70-71	0.6216598656083079
1105	72-73	0.7606297777011406
1105	74-75	0.6245498995971843
1105	76-77	0.5037050804136314
1105	78-79	0.42521654548914967
1105	80-81	0.49435049750548643
1105	82-83	0.6209095312865571
1105	84-85	0.6618099408807581
1105	86-87	0.5613217753618258
1105	88-89	0.5368595002467345
1105	90-91	0.5122702141994253
1105	92-93	0.7899575240158931
1105	94-95	0.6541356054138561
1105	96-97	0.4744840009611657
1105	98-99	0.6325585153508051
1105	100-101	0.5125621405661178
1106	1	0.14075943055465245
1106	2	0.10349439677401051
1106	3	0.06188641456765254
1106	4	0.04722215116711226
1106	5	0.06876109518391615
1106	6	0.07175387083792373
1106	7	0.0017883498735358216
1106	8	0.08305123044011964
1106	9	0.09812555272119994
1106	10-11	0.005603598652946573
1106	12-13	0.016037660362101747
1106	14-15	0.06821920951490767
1106	16-17	0.07868283411693255
1106	18-19	0.23039278809084607
1106	20-21	0.28712943567445137
1106	22-23	0.060231268487200396
1106	24-25	0.13057308660088296
1106	26-27	0.0886720982510667
1106	28-29	0.07273223091447534
1106	30-31	0.13078582350851775
1106	32-33	0.29162428413194164
1106	34-35	0.2222239946958524
1106	36-37	0.2531428134807143
1106	38-39	0.2460219396969805
1106	40-41	0.29984284207277767
1106	42-43	0.45324163548683316
1106	44-45	0.21939513783436126
1106	46-47	0.036552712847644386
1106	48-49	-0.0729789468305242
1106	50-51	0.017897365261546838
1106	52-53	-0.02648260212495046
1106	54-55	-0.2586240506537578
1106	56-57	-0.06588877322836595
1106	58-59	-0.1877453947478287
1106	60-61	-0.11641566672791015
1106	62-63	2.2770768942592667E-4
1106	64-65	0.07333365467193431
1106	66-67	0.24486087856322314
1106	68-69	0.3256514402620141
1106	70-71	0.1935894341670874
1106	72-73	-0.05078857922282509
1106	74-75	0.1925322917368817
1106	76-77	0.34014654157419244
1106	78-79	0.15086954417586185
1106	80-81	0.3975242900051299
1106	82-83	0.15481925452212408
1106	84-85	0.2494625504434822
1106	86-87	0.47455442286413785
1106	88-89	0.2998416491839393
1106	90-91	0.3406251925544055
1106	92-93	0.26348736496146685
1106	94-95	0.4446847566371588
1106	96-97	0.4121317464740777
1106	98-99	0.5422185182692338
1106	100-101	0.49936840546227046
1107	1	0.13728129949031143
1107	2	0.12160157695015528
1107	3	0.19371206018640663
1107	4	0.1841679639749927
1107	5	0.1163204323984246
1107	6	0.14404824313229625
1107	7	0.14228693175142837
1107	8	0.28748472797706626
1107	9	0.08976609608588149
1107	10-11	0.22920413604659728
1107	12-13	0.29732591234000694
1107	14-15	0.3837140594925188
1107	16-17	0.4371169287579235
1107	18-19	0.4371624552053319
1107	20-21	0.3783893252102075
1107	22-23	0.36770999665558435
1107	24-25	0.37679640351385757
1107	26-27	0.35876464937810226
1107	28-29	0.48796584787222486
1107	30-31	0.33836904488484265
1107	32-33	0.481510834363327
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2316	72-73	-0.15834176852956716
2316	74-75	-0.03745375696954767
2316	76-77	-0.5281265755140865
2316	78-79	-0.162849911723697
2316	80-81	-0.003878020164954421
2316	82-83	0.025682593394940056
2316	84-85	-0.0670112601061561
2316	86-87	-0.1580598033519962
2316	88-89	-0.06530781430675603
2316	90-91	8.958066164908018E-4
2316	92-93	-0.007095922209497729
2316	94-95	-0.0071935388430297564
2316	96-97	-0.1064255256376967
2316	98-99	-0.03708843070596046
2316	100-101	0.06855553744561149
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	5.0
5	7.0
6	14.0
7	26.0
8	62.0
9	136.0
10	278.0
11	520.0
12	778.0
13	1157.0
14	1309.0
15	1713.0
16	2157.0
17	3442.0
18	3427.0
19	1996.0
20	1515.0
21	1535.0
22	2070.0
23	2284.0
24	2574.0
25	3000.0
26	2685.0
27	2379.0
28	3025.0
29	3851.0
30	5049.0
31	6517.0
32	8478.0
33	11367.0
34	15576.0
35	24558.0
36	40468.0
37	90313.0
38	182190.0
39	64341.0
40	66.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.245333768033255	21.851210367593122	15.222512022169695	22.68094384220393
2	15.029610630898954	22.69924277457825	40.93152783521536	21.339618759307434
3	16.805062094592394	28.685949672414356	32.504522637634864	22.00446559535839
4	12.123774612185516	20.336496435142827	39.67230618445156	27.867422768220095
5	12.108476565126072	38.6470172407822	36.215013720509056	13.029492473582671
6	26.767317686316773	38.90390754393721	20.44895705796431	13.879817711781712
7	23.557309354188778	34.355462025664586	25.05627751486643	17.030951105280206
8	23.296955819349687	34.66593056016754	23.51147246425232	18.525641156230456
9	25.033460929653618	16.492927877181582	22.88747960258398	35.58613159058083
10-11	22.079120583615655	27.56498143096659	30.877053238019762	19.478844747397993
12-13	23.38027709927863	26.24273994593284	30.136329096646573	20.240653858141954
14-15	21.481633139494022	27.70160357976289	28.292390762940535	22.524372517802558
16-17	20.046366938498615	30.143501192787454	29.294595073614644	20.515536795099283
18-19	19.695560632713992	28.796655730290844	31.40101391510371	20.10676972189146
20-21	21.177559888443195	27.930250513882466	30.987061773867268	19.90512782380707
22-23	20.659093733384125	28.322919871004803	30.71957805614917	20.298408339461897
24-25	20.44661428358978	28.504230235642357	31.28194576579183	19.767209714976033
26-27	20.256402159840853	28.51704984726083	31.28295809782437	19.943589895073945
28-29	19.983945937741613	27.96727660558449	31.618353378840215	20.430424077833678
30-31	20.516226292093627	28.184651740786766	31.24075620836474	20.058365758754864
32-33	19.985148802278406	28.000619309205398	31.519579032716432	20.494652855799757
34-35	20.108105464969633	28.23679701395836	31.209595429661157	20.44550209141085
36-37	20.171517745638713	28.2311608567127	31.26251647641962	20.33480492122896
38-39	20.007802457264738	28.106366030993886	31.56582890413164	20.320002607609737
40-41	20.350153095212388	27.93249142850158	31.44390277690065	20.273452699385377
42-43	20.531667179360767	28.039953470463725	31.04624229176301	20.382137058412493
44-45	20.35646840004808	28.29287124315757	30.758692202228282	20.591968154566068
46-47	20.836533427315935	28.01357179381141	30.253264095862253	20.896630683010404
48-49	20.574040837613143	28.06012170203577	30.33200168679755	21.03383577355354
50-51	20.5134342831416	28.242858103249123	30.358994521982353	20.884713091626924
52-53	20.51608263678237	28.44973526649568	30.47888345410505	20.555298642616897
54-55	20.75830513515771	28.513295753874235	29.87740159838328	20.850997512584772
56-57	20.747202421817544	28.966877244734363	29.331637028873164	20.95428330457493
58-59	20.321021205163884	28.991629165300047	30.236355376463468	20.4509942530726
60-61	20.87574600982151	29.550290662437366	29.33271401403451	20.241249313706618
62-63	20.432073737976726	29.8067108241531	29.28931031602281	20.47190512184736
64-65	20.541190251852605	29.576612595176854	29.18770531462477	20.69449183834577
66-67	20.48191666539337	29.738872675600103	29.04599650607871	20.733214152927815
68-69	20.538547338076732	29.494507061621754	29.088686635123228	20.878258965178283
70-71	20.560616292891915	29.15066193629481	29.338389656742457	20.950332114070818
72-73	20.449044702776046	29.036431275237245	29.495845913756362	21.018678108230347
74-75	20.603832551013756	29.081813514570705	28.983717973792295	21.330635960623248
76-77	20.401536063887217	29.27831481160808	29.149868090004382	21.17028103450032
78-79	20.52187039719355	29.085967946641567	29.063253128635136	21.328908527529748
80-81	20.43338701774438	29.097867598147225	29.057222285040794	21.411523099067605
82-83	20.604175071003915	29.12240382382064	29.06168885411701	21.21173225105844
84-85	20.57704550663373	28.856044207899362	28.896686954085922	21.67022333138099
86-87	20.386217349629785	28.9616156018934	29.051457278856546	21.60070976962027
88-89	20.721108890559396	28.608854908336035	29.002238886546106	21.667797314558467
90-91	20.60958989225275	28.658242185828865	29.10367489625584	21.628493025662547
92-93	20.61468288002347	28.809605782374597	28.888343373309894	21.687367964292044
94-95	20.552548429220714	28.41520480941021	29.10255443894628	21.929692322422795
96-97	20.51488121612523	28.701358097502293	29.019466437685704	21.76429424868677
98-99	20.50426690515431	28.45421709573391	28.955978250905023	22.08553774820676
100-101	20.419135083158814	28.65149082849157	28.719431700579385	22.209942387770234
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	872.0
1	509.5
2	165.0
3	159.0
4	115.0
5	108.0
6	154.5
7	273.5
8	420.0
9	455.0
10	352.5
11	277.5
12	283.5
13	306.0
14	327.0
15	347.5
16	389.5
17	430.5
18	463.0
19	498.5
20	574.5
21	679.0
22	766.0
23	869.5
24	1058.0
25	1302.5
26	1685.5
27	2274.5
28	2934.0
29	4003.5
30	5592.0
31	7632.0
32	10370.5
33	13666.5
34	16772.5
35	19302.5
36	22120.5
37	24430.5
38	24786.0
39	24499.5
40	23934.0
41	23310.5
42	22979.5
43	22549.0
44	21795.0
45	21108.0
46	20186.5
47	19035.0
48	17904.0
49	16292.0
50	14650.5
51	13043.0
52	11228.5
53	9493.5
54	8177.0
55	6774.5
56	5382.0
57	4299.5
58	3507.5
59	2926.0
60	2371.0
61	1827.0
62	1390.0
63	1011.5
64	770.5
65	637.5
66	502.5
67	386.0
68	311.0
69	244.5
70	214.0
71	180.5
72	135.0
73	107.5
74	96.5
75	79.5
76	51.5
77	41.0
78	39.0
79	33.5
80	26.0
81	20.0
82	9.5
83	5.5
84	3.5
85	1.0
86	2.5
87	3.5
88	3.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022612865701986878
2	0.0
3	0.001426036575800974
4	0.022001707169500742
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.018597554143553E-4
16-17	8.148780433148424E-4
18-19	3.055792662430659E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.055792662430659E-4
28-29	0.006009725569446962
30-31	2.037195108287106E-4
32-33	3.055792662430659E-4
34-35	0.011000853584750371
36-37	0.003666951194916791
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0019353353528727508
62-63	0.010593414563092951
64-65	0.00173161584204404
66-67	0.0037688109503311463
68-69	0.0027502133961875928
70-71	1.018597554143553E-4
72-73	0.005907865814032607
74-75	0.004685548749060344
76-77	0.0012223170649722636
78-79	4.074390216574212E-4
80-81	0.008046920677734068
82-83	0.010797134073921662
84-85	0.00173161584204404
86-87	0.0015278963312153294
88-89	4.074390216574212E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	1.018597554143553E-4
98-99	0.0
100-101	6.111585324861318E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	490871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.41237420014622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.67435076250466	34.42148647516053
2	18.712227486982517	19.61504538331979
3	7.34765523832252	11.553241675338734
4	3.3136255084457336	6.946999204312301
5	1.6743371246550898	4.387799195730971
6	0.9674569213496723	3.0424028510580277
7	0.5965461618429472	2.1886480463521476
8	0.3653836178603579	1.5320498324720246
9	0.2516334876428543	1.1869837664062635
>10	1.0187231108060923	9.426720241903357
>50	0.055423011632390565	1.9499327918243565
>100	0.019124841893149877	1.4702231314109426
>500	0.0019515144788928445	0.738282354426338
>1k	0.0015612115831142755	1.5401850502842613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	2809	0.572248105917848	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1905	0.38808566812869366	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1677	0.34163761965974765	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	1138	0.23183280332307266	RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT	869	0.1770322549101495	RNA PCR Primer, Index 27 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	803	0.16358676719545462	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG	713	0.14525201122087067	RNA PCR Primer, Index 27 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	659	0.13425115763612028	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	565	0.11510152361822149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.037195108287106E-4	0.0
5	0.0	0.0	0.0	2.037195108287106E-4	0.0
6	0.0	0.0	0.0	2.037195108287106E-4	0.0
7	0.0	0.0	0.0	2.037195108287106E-4	0.0
8	0.0	0.0	0.0	4.074390216574212E-4	0.0
9	0.0	0.0	0.0	4.074390216574212E-4	0.0
10-11	0.0	0.0	0.0	4.074390216574212E-4	0.0
12-13	0.0	0.0	0.0	4.074390216574212E-4	0.0
14-15	0.0	0.0	0.0	4.074390216574212E-4	0.0
16-17	0.0	0.0	0.0	4.074390216574212E-4	0.0
18-19	0.0	0.0	0.0	4.074390216574212E-4	0.0
20-21	0.0	0.0	0.0	9.167377987291977E-4	0.0
22-23	0.0	0.0	0.0	0.0011204573095579084	0.0
24-25	0.0	0.0	0.0	0.0016297560866296848	0.0
26-27	0.0	0.0	0.0	0.0018334755974583955	0.0
28-29	0.0	0.0	0.0	0.0038706707057455016	0.0
30-31	0.0	0.0	0.0	0.009167377987291978	0.0
32-33	0.0	0.0	0.0	0.0163994206217112	0.0
34-35	0.0	0.0	0.0	0.027909572983533353	0.0
36-37	0.0	0.0	0.0	0.0412532009428139	0.0
38-39	0.0	0.0	0.0	0.05439310939126573	0.0
40-41	0.0	0.0	0.0	0.06661628004098837	0.0
42-43	0.0	2.037195108287106E-4	0.0	0.08770124941175991	0.0
44-45	0.0	2.037195108287106E-4	0.0	0.1132680480207631	0.0
46-47	0.0	2.037195108287106E-4	0.0	0.1417887795367826	0.0
48-49	0.0	2.037195108287106E-4	0.0	0.18406057803374004	0.0
50-51	0.0	2.037195108287106E-4	0.0	0.21757243756506292	0.0
52-53	0.0	2.037195108287106E-4	0.0	0.25648286413334664	0.0
54-55	0.0	2.037195108287106E-4	0.0	0.3094499369488114	0.0
56-57	0.0	2.037195108287106E-4	0.0	0.36323188780759097	0.0
58-59	0.0	2.037195108287106E-4	0.0	0.4161989606230557	0.0
60-61	0.0	2.037195108287106E-4	0.0	0.4719162468347081	0.0
62-63	0.0	2.037195108287106E-4	0.0	0.5174475575049249	0.0
64-65	0.0	2.037195108287106E-4	0.0	0.5772392339331516	0.0
66-67	0.0	2.037195108287106E-4	0.0	0.678284111304192	0.0
68-69	0.0	2.037195108287106E-4	0.0	0.8011269763339044	0.0
70-71	0.0	2.037195108287106E-4	0.0	0.9280442315801911	0.0
72-73	0.0	2.037195108287106E-4	0.0	1.0641288648137697	0.0
74-75	0.0	2.037195108287106E-4	0.0	1.2178352357340319	0.0
76-77	0.0	2.037195108287106E-4	0.0	1.382135021217387	0.0
78-79	0.0	2.037195108287106E-4	0.0	1.573631361396375	0.0
80-81	0.0	2.037195108287106E-4	0.0	1.7655351405970205	0.0
82-83	0.0	2.037195108287106E-4	0.0	1.9805610842767245	0.0
84-85	0.0	2.037195108287106E-4	0.0	2.212903186376869	0.0
86-87	0.0	2.037195108287106E-4	0.0	2.493017513766346	0.0
88-89	0.0	2.037195108287106E-4	0.0	2.8126534262565928	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1265	0.0	50.698494	1
GTATCAA	2170	0.0	40.719746	1
TCAACGC	2610	0.0	33.1203	4
ATCAACG	2645	0.0	32.68203	3
TATCAAC	2705	0.0	32.48387	2
CAACGCA	2665	0.0	32.258537	5
AACGCAG	2740	0.0	31.375553	6
CGCAGAG	3160	0.0	27.656298	8
ACGCAGA	3140	0.0	27.378664	7
GCAGAGT	3485	0.0	24.940872	9
GAGTACT	1845	0.0	23.94264	12-13
GTACTTT	1980	0.0	22.071419	14-15
GTACATG	2235	0.0	21.68076	1
CAGAGTA	3355	0.0	21.447834	10-11
ACATGGG	2190	0.0	21.037346	3
GTATAGG	230	8.981442E-8	20.654942	1
TACATGG	2300	0.0	20.444225	2
AGAGTAC	3095	0.0	20.410532	10-11
CTCGTAT	770	0.0	20.047304	38-39
TGCCGTC	795	0.0	19.715607	44-45
>>END_MODULE