FastQCFastQC Report
Fri 10 Feb 2017
SRR2031734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549871
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.20563001694979777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.18569287379401253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.054.2612721
GTATCAA42000.039.6993181
TCAACGC49450.032.9498184
ATCAACG50300.032.4874533
CAACGCA51050.032.009135
TATCAAC51650.031.7302842
AACGCAG53200.030.977396
ACGCAGA61600.026.7540667
CGCAGAG62950.026.1845388
GCAGAGT71150.022.4991539
GTGGTAT11650.022.4265251
GTACATG45650.021.332331
GAGTACT39900.020.77712-13
CAGAGTA67500.020.26879710-11
TACATGG45600.020.1056292
TGGTATC13250.019.3598982
GTACTTT42550.019.09254814-15
ACATGGG47300.018.9812973
AGAGTAC63400.018.17028410-11
AGTACTT40350.017.07201612-13
Produced by FastQC (version 0.11.5)