FastQCFastQC Report
Fri 10 Feb 2017
SRR2031734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549871
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41060.2649252744260651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39180.2527952326354903No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT37320.24079423384268756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.11820338595921855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.057.219371
GTATCAA45350.038.7564771
TCAACGC54300.032.3653074
ATCAACG54650.031.98423
CAACGCA55600.031.6919485
TATCAAC55950.031.4108352
AACGCAG57700.030.6208276
GTGGTAT11650.027.7271
ACGCAGA67050.026.1383047
CGCAGAG68250.025.4003688
GTACATG42450.023.1639441
TGGTATC13700.022.8823812
GCAGAGT75050.022.0230949
TACATGG43300.021.9391562
GAGTACT47250.021.21018612-13
ACATGGG43650.020.675083
CAGAGTA72700.020.61241110-11
CATGGGG27000.020.2307454
GTACTTT51900.019.17257714-15
AGAGTAC67950.017.78943410-11
Produced by FastQC (version 0.11.5)