FastQCFastQC Report
Fri 10 Feb 2017
SRR2031732_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031732_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921703
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49130.25565865276788347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44580.23198173703220532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.10443861512418931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.046.9298061
GTATCAA66400.035.1994321
TCAACGC79850.029.2101574
CAACGCA80150.028.9822855
ATCAACG80000.028.9178683
TATCAAC80150.028.8645022
AACGCAG83700.027.8054566
ACGCAGA91600.025.148137
CGCAGAG92600.024.4687678
GAGTACT52600.022.98479712-13
GCAGAGT100200.021.9017549
GTACATG53950.021.0448131
CAGAGTA93200.020.41280210-11
GTACTTT58850.020.01958814-15
TACATGG55200.019.8797782
AGAGTAC80300.019.61028910-11
GTGGTAT22750.019.2107831
ACATGGG58100.018.559963
CATGGGG35250.017.519094
TGGTATC25000.017.1017722
Produced by FastQC (version 0.11.5)