##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031732_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921703 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.097945416123096 33.0 31.0 34.0 30.0 34.0 2 32.395568409894764 34.0 31.0 34.0 31.0 34.0 3 32.50070068059424 34.0 31.0 34.0 31.0 34.0 4 35.91479588677335 37.0 37.0 37.0 35.0 37.0 5 35.917832776448805 37.0 37.0 37.0 35.0 37.0 6 35.945795994490304 37.0 37.0 37.0 35.0 37.0 7 35.93033574907257 37.0 37.0 37.0 35.0 37.0 8 35.92258689297982 37.0 37.0 37.0 35.0 37.0 9 37.71209442874367 39.0 39.0 39.0 35.0 39.0 10-11 37.643603876353424 39.0 38.0 39.0 35.0 39.0 12-13 37.5997552691545 39.0 38.0 39.0 35.0 39.0 14-15 39.014157494680504 41.0 39.0 41.0 36.0 41.0 16-17 38.94347825860708 41.0 39.0 41.0 36.0 41.0 18-19 38.95583500676223 41.0 39.0 41.0 36.0 41.0 20-21 38.959766415517905 41.0 39.0 41.0 36.0 41.0 22-23 38.864211327140566 41.0 39.0 41.0 35.5 41.0 24-25 38.7981774498973 41.0 39.0 41.0 35.0 41.0 26-27 38.68648745409671 41.0 39.0 41.0 35.0 41.0 28-29 38.57613013041038 40.0 38.5 41.0 35.0 41.0 30-31 38.41903899822189 40.0 38.0 41.0 34.5 41.0 32-33 38.21711029227721 40.0 38.0 41.0 34.0 41.0 34-35 38.05450113779288 40.0 38.0 41.0 34.0 41.0 36-37 37.89466062133431 40.0 38.0 41.0 33.5 41.0 38-39 37.711420287109924 40.0 38.0 41.0 33.0 41.0 40-41 37.61449870245298 40.0 38.0 41.0 33.0 41.0 42-43 37.58590661512211 40.0 38.0 41.0 33.0 41.0 44-45 37.44601897379564 40.0 38.0 41.0 32.5 41.0 46-47 37.34991775524105 40.0 38.0 41.0 32.5 41.0 48-49 37.374973135807146 40.0 38.0 41.0 33.0 41.0 50-51 36.81510722520598 39.5 37.0 40.5 31.5 41.0 52-53 36.82538795016712 39.5 37.0 40.5 31.5 41.0 54-55 37.03265072698539 40.0 37.0 41.0 32.0 41.0 56-57 36.89566597960247 40.0 37.0 41.0 31.0 41.0 58-59 36.70237804697188 40.0 36.0 41.0 31.0 41.0 60-61 36.452543655289084 39.0 35.5 41.0 31.0 41.0 62-63 36.16189026087798 39.0 35.0 41.0 30.5 41.0 64-65 35.87367584897354 39.0 35.0 41.0 30.0 41.0 66-67 35.56907180766227 38.0 35.0 41.0 30.0 41.0 68-69 35.21969758073958 37.0 35.0 40.0 29.0 41.0 70-71 34.85186576697856 37.0 35.0 39.5 29.0 41.0 72-73 34.48117242883005 36.0 35.0 39.0 29.0 41.0 74-75 34.07323478185755 36.0 34.5 39.0 28.5 40.5 76-77 33.698730761205034 35.0 34.0 37.5 28.0 39.5 78-79 33.32778113995764 35.0 34.0 37.0 27.5 39.0 80-81 32.986815600537646 35.0 34.0 37.0 27.0 39.0 82-83 32.678255172625526 35.0 34.0 36.0 26.0 38.0 84-85 32.37351011056339 35.0 34.0 36.0 26.0 37.0 86-87 32.1190818768561 35.0 34.0 35.5 26.0 37.0 88-89 31.864931781862232 35.0 33.5 35.0 25.0 36.0 90-91 31.6477954709963 35.0 33.0 35.0 25.0 36.0 92-93 31.45604341565788 35.0 33.0 35.0 24.0 36.0 94-95 31.224490985339564 35.0 33.0 35.0 23.5 36.0 96-97 30.981787508267406 35.0 33.0 35.0 20.0 35.0 98-99 30.747378236907576 35.0 33.0 35.0 18.5 35.0 100-101 30.18082294714636 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.26477676905135894 1101 2 -0.07367778340267961 1101 3 0.07805551085554185 1101 4 0.1170113211935444 1101 5 -0.12744795084533678 1101 6 0.08479086756616994 1101 7 0.08577173517286951 1101 8 0.08668181361515792 1101 9 0.18044446685839688 1101 10-11 -0.0017509322712427888 1101 12-13 0.053877198877813726 1101 14-15 0.04580472746467734 1101 16-17 0.017836875178673495 1101 18-19 0.12911789899773396 1101 20-21 0.21709483495257587 1101 22-23 0.13272448697713202 1101 24-25 -0.087578038778787 1101 26-27 -0.14826088602255538 1101 28-29 -0.21248446159233936 1101 30-31 -0.20608428352990416 1101 32-33 -0.23271458201874395 1101 34-35 -0.16925423693032826 1101 36-37 -0.37909762783147727 1101 38-39 -0.21453151626059963 1101 40-41 -0.38684655298912674 1101 42-43 -0.3617808280487367 1101 44-45 -0.5832997313009258 1101 46-47 -0.4302791151823655 1101 48-49 -0.23507216598342495 1101 50-51 -0.19346702344660116 1101 52-53 -0.3091077523850956 1101 54-55 -0.17109456626636899 1101 56-57 -0.33245300500430375 1101 58-59 -0.4980160436927221 1101 60-61 -0.5132124662529236 1101 62-63 -0.4562472582109933 1101 64-65 -0.16115471002483162 1101 66-67 -0.4161831569478025 1101 68-69 -0.5141167401149502 1101 70-71 -0.6788669098217994 1101 72-73 -0.3433376585093413 1101 74-75 -0.16529045152691424 1101 76-77 -0.4428366708036009 1101 78-79 -0.44825701307158283 1101 80-81 -0.5955542832497898 1101 82-83 -0.5075385974083062 1101 84-85 -0.5566953577476887 1101 86-87 -0.6082663646986077 1101 88-89 -0.6104531491088494 1101 90-91 -0.927189043029756 1101 92-93 -0.9152494152802007 1101 94-95 -0.8008950788654907 1101 96-97 -0.9638248380077599 1101 98-99 -0.9741303794269349 1101 100-101 -0.8805966342594367 1102 1 0.0030632992304688855 1102 2 0.05390042254622074 1102 3 0.11813109760441165 1102 4 0.17947458030499774 1102 5 0.08348444591973703 1102 6 0.09316961638633359 1102 7 0.009288787366557472 1102 8 -0.028321540883233354 1102 9 0.040890797767644926 1102 10-11 0.17035790341986257 1102 12-13 0.11189445003459042 1102 14-15 0.30902270065561055 1102 16-17 0.19330732195172828 1102 18-19 0.10311409659210824 1102 20-21 0.12401605902729784 1102 22-23 0.23560512642718834 1102 24-25 0.0325191551688917 1102 26-27 -0.05926133392978983 1102 28-29 0.1167054414636155 1102 30-31 0.047780310075978605 1102 32-33 0.10346440401924184 1102 34-35 0.004803283585943063 1102 36-37 0.008074924105578418 1102 38-39 -0.1268629983721823 1102 40-41 -0.12493827593254991 1102 42-43 0.08790073489761596 1102 44-45 -0.2554352876382424 1102 46-47 -0.08051104654683172 1102 48-49 -0.09114380807287148 1102 50-51 0.029394892327609057 1102 52-53 -0.06435797489695005 1102 54-55 0.06034390800396494 1102 56-57 0.036441279276452576 1102 58-59 -0.21002072653403303 1102 60-61 -0.2578957304778271 1102 62-63 -0.21974493313030052 1102 64-65 -0.13561784610597272 1102 66-67 -0.1286165235377652 1102 68-69 -0.17968651464268248 1102 70-71 -0.19615401451483194 1102 72-73 -0.22285983952922095 1102 74-75 -0.42431808913343616 1102 76-77 -0.05555992756978867 1102 78-79 -0.22747200277740376 1102 80-81 -0.1675181738527627 1102 82-83 -0.12234452670048057 1102 84-85 -0.19754161406981297 1102 86-87 -0.30512236348368305 1102 88-89 -0.23740332375422568 1102 90-91 -0.1819292664451133 1102 92-93 0.03605691674401257 1102 94-95 -0.21470638496683492 1102 96-97 -0.5736174742590237 1102 98-99 -0.3310628492412597 1102 100-101 -0.49607057970702684 1103 1 -0.04173942873769221 1103 2 0.15827588984906527 1103 3 0.10676996596438926 1103 4 0.1609337320936035 1103 5 0.1440501362746076 1103 6 0.06161667988765629 1103 7 0.10303352008013889 1103 8 0.17285314502442617 1103 9 0.23046124650803534 1103 10-11 0.18664165186938675 1103 12-13 0.23967881867332608 1103 14-15 0.3631347496138204 1103 16-17 0.24206780790346727 1103 18-19 0.21114857219727412 1103 20-21 0.10397930147158263 1103 22-23 0.10219745682596226 1103 24-25 0.30066185055010664 1103 26-27 0.17738568981027925 1103 28-29 0.12785101953615197 1103 30-31 0.12481450908108371 1103 32-33 0.005709510575094612 1103 34-35 -0.015386900569197337 1103 36-37 -0.06205453769120339 1103 38-39 0.1325635535054559 1103 40-41 0.3089613312089199 1103 42-43 0.12197926770713252 1103 44-45 0.10013950026569773 1103 46-47 -0.018021581119676 1103 48-49 0.168311916468042 1103 50-51 0.15052735350636937 1103 52-53 0.11042301403472976 1103 54-55 0.04426328772831312 1103 56-57 -0.098119790992925 1103 58-59 0.025136330441256405 1103 60-61 -0.04713998562409927 1103 62-63 -0.022338382894979247 1103 64-65 -0.07025487334675518 1103 66-67 -0.2293853852852763 1103 68-69 -0.3408897897603467 1103 70-71 -0.31003977004358774 1103 72-73 -0.41008729122145837 1103 74-75 -0.49901307273191975 1103 76-77 -0.34546921139170905 1103 78-79 -0.283115616557275 1103 80-81 -0.3049527514542163 1103 82-83 -0.3627915599486684 1103 84-85 -0.5119656592593493 1103 86-87 -0.30396518117398585 1103 88-89 -0.34863598762895975 1103 90-91 -0.4343505974965005 1103 92-93 -0.8820203022151922 1103 94-95 -0.5453742012525282 1103 96-97 -0.4637737532966284 1103 98-99 -0.48283417013623264 1103 100-101 -0.4427137785867963 1104 1 -0.05431757883530253 1104 2 0.08366365436501155 1104 3 0.2227966049541834 1104 4 0.10565412154674192 1104 5 0.09195213049211759 1104 6 0.20328561177453963 1104 7 0.1745424038619845 1104 8 0.08746257987257877 1104 9 0.14778894562089562 1104 10-11 0.13841511337299295 1104 12-13 0.21711413633956766 1104 14-15 0.2578699659629464 1104 16-17 0.28605426600513084 1104 18-19 0.29808051674468317 1104 20-21 0.22525404385426384 1104 22-23 0.27626933432285483 1104 24-25 0.21494488775737608 1104 26-27 0.13548426395711033 1104 28-29 0.21908894179379246 1104 30-31 0.20227680346086885 1104 32-33 0.22284219481986867 1104 34-35 0.20056169128753965 1104 36-37 0.3186476393913651 1104 38-39 0.2706276963049348 1104 40-41 0.23914827365585012 1104 42-43 0.25292238184951543 1104 44-45 0.25085283870607356 1104 46-47 0.29703451397015357 1104 48-49 0.3242239353146772 1104 50-51 0.3279641126763835 1104 52-53 0.333681059685091 1104 54-55 0.44653550953867693 1104 56-57 0.35762089483338144 1104 58-59 0.4417834338423816 1104 60-61 0.317347333261246 1104 62-63 0.2614267837840458 1104 64-65 0.3349889055348001 1104 66-67 0.3517918702562781 1104 68-69 0.3811213551231418 1104 70-71 0.4249685906783185 1104 72-73 0.2772495099581107 1104 74-75 0.3779854692899818 1104 76-77 0.26013947387447445 1104 78-79 0.19611128437434644 1104 80-81 0.18748759088234124 1104 82-83 0.1903021130853233 1104 84-85 0.2647577614702996 1104 86-87 0.11915736312136715 1104 88-89 0.2589568246213503 1104 90-91 0.3795718578374796 1104 92-93 0.24677497129985326 1104 94-95 0.3840765446227117 1104 96-97 0.2612223340502595 1104 98-99 0.2897603719186108 1104 100-101 0.2875788054940607 1105 1 -0.004525860198882015 1105 2 0.2037090926946803 1105 3 0.1214334550640217 1105 4 0.10131749701915993 1105 5 0.21990635955601334 1105 6 0.3004187589555727 1105 7 0.19802066239910232 1105 8 0.2794408638916437 1105 9 0.37702652585548435 1105 10-11 0.3810826839781001 1105 12-13 0.32129438220010087 1105 14-15 0.30654047906200077 1105 16-17 0.35811452141467726 1105 18-19 0.2767350298652076 1105 20-21 0.2883211847533147 1105 22-23 0.1559901997433215 1105 24-25 0.17452281288337446 1105 26-27 0.1902577026515786 1105 28-29 0.11359905812557258 1105 30-31 0.24645813565690133 1105 32-33 0.1938561481959553 1105 34-35 0.1307743582278036 1105 36-37 0.12035050383010315 1105 38-39 0.12543996143516978 1105 40-41 0.1833123049530414 1105 42-43 0.23532341132005996 1105 44-45 0.36401815214739486 1105 46-47 0.379901125381231 1105 48-49 0.3338088344464154 1105 50-51 0.293076313699423 1105 52-53 0.45719003067054587 1105 54-55 0.4082646472028628 1105 56-57 0.485240841026247 1105 58-59 0.45919430739024847 1105 60-61 0.5652712254001955 1105 62-63 0.49028179956943774 1105 64-65 0.563659512514711 1105 66-67 0.5554777664026105 1105 68-69 0.548585338406987 1105 70-71 0.48783120544764813 1105 72-73 0.3113091457540662 1105 74-75 0.3870080892093455 1105 76-77 0.5315386069519192 1105 78-79 0.46138301577855856 1105 80-81 0.37796277549232116 1105 82-83 0.4940616386383496 1105 84-85 0.5227862502837226 1105 86-87 0.44352675206435066 1105 88-89 0.3294046764934677 1105 90-91 0.1524080384427684 1105 92-93 0.3350008378871223 1105 94-95 0.39487921244813506 1105 96-97 0.3896275264657625 1105 98-99 0.29461602341145365 1105 100-101 0.3335093390323216 1106 1 -0.03443231504759581 1106 2 0.09741909494972134 1106 3 0.18050375778328487 1106 4 0.14882684764629772 1106 5 0.07831596238440142 1106 6 0.19229103573931638 1106 7 0.15601606419743064 1106 8 0.1559464898199039 1106 9 0.2023551262033365 1106 10-11 0.1517716755100409 1106 12-13 0.1621989371154271 1106 14-15 0.19686209397946186 1106 16-17 0.23209813481322783 1106 18-19 0.09391536542506174 1106 20-21 0.15925725834527782 1106 22-23 0.19016340472099102 1106 24-25 0.3170401727794854 1106 26-27 0.2531641600922967 1106 28-29 0.22063166284871016 1106 30-31 0.20684044217084363 1106 32-33 0.2782726120388972 1106 34-35 0.2736649656103225 1106 36-37 0.24165334491739543 1106 38-39 0.2988033748871146 1106 40-41 0.2830610756987326 1106 42-43 0.25803545606828493 1106 44-45 0.32184831417308857 1106 46-47 0.446389073893819 1106 48-49 0.4048110687473354 1106 50-51 0.44157016937187876 1106 52-53 0.46920587813674075 1106 54-55 0.5081456097666646 1106 56-57 0.5378826685910951 1106 58-59 0.5236766986624772 1106 60-61 0.37258026654295406 1106 62-63 0.46162954699493497 1106 64-65 0.5624552061718404 1106 66-67 0.659425895369985 1106 68-69 0.6051874125225325 1106 70-71 0.530618586572217 1106 72-73 0.46885878896576116 1106 74-75 0.45108483303974367 1106 76-77 0.5518789749635715 1106 78-79 0.6690090580851304 1106 80-81 0.63328900416343 1106 82-83 0.6550434091274369 1106 84-85 0.7509710363014577 1106 86-87 0.6370286818056883 1106 88-89 0.720289078636533 1106 90-91 0.7456288661709145 1106 92-93 0.7452840653373975 1106 94-95 0.7537518106394394 1106 96-97 0.7513383607516531 1106 98-99 0.9291879422404925 1106 100-101 0.6892679775084325 1107 1 0.1634412207944962 1107 2 0.2865372006337239 1107 3 0.22425648049610203 1107 4 0.25588086219728723 1107 5 0.14552303241291753 1107 6 0.24802844476169383 1107 7 0.1981714620561732 1107 8 0.23882688588046364 1107 9 0.2807583984800459 1107 10-11 0.23772492016946245 1107 12-13 0.23144710874166918 1107 14-15 0.32031141996808543 1107 16-17 0.4476643844383261 1107 18-19 0.34082453504684906 1107 20-21 0.25090122725647745 1107 22-23 0.32094861813418163 1107 24-25 0.3370579645626961 1107 26-27 0.24806004898253775 1107 28-29 0.25706817631061796 1107 30-31 0.313250166174285 1107 32-33 0.43897645281126785 1107 34-35 0.4826217238415751 1107 36-37 0.45508315558622314 1107 38-39 0.43614345579833724 1107 40-41 0.517693439915945 1107 42-43 0.4543574697018684 1107 44-45 0.532644981640459 1107 46-47 0.5231712717965351 1107 48-49 0.6316077877850859 1107 50-51 0.4420478740011262 1107 52-53 0.45808296012143757 1107 54-55 0.6382156233513854 1107 56-57 0.6095817906731256 1107 58-59 0.5679271434570623 1107 60-61 0.6236662981610337 1107 62-63 0.67408528806817 1107 64-65 0.6226001868125834 1107 66-67 0.7131395060162831 1107 68-69 0.7124249769346207 1107 70-71 0.7132571446642189 1107 72-73 0.7165235325998722 1107 74-75 0.6311506395666555 1107 76-77 0.6030473156321108 1107 78-79 0.6884544864382534 1107 80-81 0.7010060103125184 1107 82-83 0.6458896655923922 1107 84-85 0.6156910566039073 1107 86-87 0.7236961084569984 1107 88-89 0.6281789405771789 1107 90-91 0.6054754849856856 1107 92-93 0.7869872044810897 1107 94-95 0.7679160243199767 1107 96-97 0.9373378151912561 1107 98-99 1.069755582908897 1107 100-101 1.0594944936438573 1108 1 -0.04847701739017651 1108 2 0.08085272200037252 1108 3 0.13850755461687214 1108 4 0.20667841294466882 1108 5 0.26745439159375195 1108 6 0.2560020226772508 1108 7 0.23087039951276012 1108 8 0.1886332868684022 1108 9 0.15971656565697145 1108 10-11 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6 1273.0 7 1375.0 8 1663.0 9 2012.0 10 2427.0 11 3025.0 12 3755.0 13 4560.0 14 5397.0 15 6362.0 16 7941.0 17 10794.0 18 10504.0 19 6266.0 20 5770.0 21 5858.0 22 6298.0 23 6761.0 24 7575.0 25 9086.0 26 10440.0 27 12359.0 28 15216.0 29 18444.0 30 22925.0 31 28813.0 32 37351.0 33 50313.0 34 72071.0 35 116110.0 36 207352.0 37 421527.0 38 592976.0 39 194323.0 40 199.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.6590974471864 19.43143300271416 14.098583298937264 23.81088625116217 2 16.38120184996211 21.476363158777993 38.585385632878214 23.55704935838169 3 18.71447764351996 25.440547574552003 30.69902890358973 25.145945878338306 4 13.820448409927142 18.695368932342426 36.91225674647432 30.57192591125611 5 14.389577905158937 36.53741532047942 34.457217300677435 14.61578947368421 6 30.144113269142903 36.13793527432891 18.750407029722417 14.967544426805764 7 25.96246580067453 32.75392689798115 23.130337009570862 18.153270291773456 8 24.65100843518929 33.69970392699193 21.794370184515532 19.854917453303248 9 25.659423698774226 16.62777667506982 21.616844237378466 36.09595538877749 10-11 24.029906801044294 26.25859220195816 29.020051422987407 20.69144957401014 12-13 25.106315360339064 24.817502276093013 28.24798798587585 21.828194377692075 14-15 22.756020439322455 26.44829774794351 26.4395694527385 24.35611235999554 16-17 21.899360547833837 28.71218398711715 27.383666088189155 22.004789376859858 18-19 21.763171608734172 27.7109281359112 29.152431080306425 21.373469175048204 20-21 22.77710369846778 26.87769155873509 28.838450329155375 21.506754413641755 22-23 22.643957881376 26.935488955415504 28.40985442620969 22.010698736998805 24-25 22.351860616743714 27.114884230635884 28.711276386967437 21.821978765652965 26-27 22.060919383632065 27.191023470548565 28.896212915926313 21.851844229893057 28-29 21.994662178972007 27.13376725380961 28.985384165506318 21.886186401712063 30-31 22.40748897703039 26.938217971263047 28.745237402478597 21.90905564922797 32-33 22.276761305753183 27.172723604671063 28.428818551299663 22.121696538276094 34-35 22.510309475755765 27.213082124883968 28.426694149917974 21.84991424944229 36-37 22.448975338351584 27.111165647879883 28.534497154926193 21.905361858842344 38-39 22.26653194011702 27.230496755960775 28.609125509555245 21.89384579436696 40-41 22.454511731815632 27.178680168136225 28.507587803663238 21.859220296384905 42-43 22.346514237606367 27.21318743377161 28.2469234079398 22.19337492068222 44-45 22.470704369119087 27.266645147121004 28.169866852370447 22.092783631389462 46-47 22.451094613633902 27.339575118173254 28.02706938666622 22.182260881526624 48-49 22.388637985612487 27.45126189742284 27.995151529047963 22.164948587916708 50-51 22.39404942193618 27.583115643766686 27.844473385773355 22.178361548523778 52-53 22.530230513034375 27.61108225523196 27.637599563636588 22.221087668097073 54-55 22.48849913968199 27.558931653295225 27.675333747647347 22.277235459375436 56-57 22.380545977280995 27.655040395482878 27.631567978308464 22.33284564892767 58-59 22.367659121771137 27.56181343191633 27.814227450298013 22.256299996014516 60-61 22.424137597790235 27.649411024921612 27.820837795265746 22.10561358202241 62-63 22.33644141638979 27.618819379955013 27.848675993086587 22.19606321056861 64-65 22.410603252666718 27.526723209635495 27.765276072308925 22.297397465388862 66-67 22.340834049328926 27.636396509241422 27.634077881910486 22.388691559519163 68-69 22.275333561908653 27.613574664405927 27.72091474592186 22.390177027763563 70-71 22.339687265061954 27.529125153890117 27.75312873002815 22.378058851019777 72-73 22.35260803612063 27.550965914700654 27.62828389767038 22.46814215150834 74-75 22.302394184903523 27.746910573721944 27.568548721071544 22.382146520302985 76-77 22.2776708752064 27.654253636815056 27.56301737240601 22.505058115572535 78-79 22.278061926318685 27.76703536503014 27.499603998365973 22.455298710285206 80-81 22.307495909711438 27.808152440104628 27.398810950510118 22.48554069967382 82-83 22.385393193144214 27.72006260387624 27.526060413730647 22.368483789248902 84-85 22.413833977987704 27.638361012179004 27.478610735312394 22.4691942745209 86-87 22.2425619989745 27.725052421388764 27.521672023444765 22.510713556191977 88-89 22.316323984000558 27.768471805334922 27.39675103174723 22.51845317891729 90-91 22.228839654153205 27.655889371127728 27.521847295202857 22.59342367951621 92-93 22.307586477884996 27.676649290401123 27.557622826815976 22.45814140489791 94-95 22.235059295992297 27.612259124684655 27.571646501895952 22.581035077427092 96-97 22.228577575552517 27.58502043220049 27.60564928399391 22.580752708253076 98-99 22.241303324053547 27.613899678263625 27.510413714638172 22.63438328304466 100-101 22.16039267927093 27.68429512236308 27.490206992592963 22.665105205773028 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4137.0 1 2337.0 2 534.0 3 498.5 4 397.0 5 355.0 6 512.0 7 926.5 8 1459.5 9 1663.0 10 1388.0 11 1094.5 12 1065.5 13 1133.5 14 1222.0 15 1324.0 16 1494.0 17 1639.5 18 1752.5 19 1884.0 20 2109.0 21 2449.5 22 2915.0 23 3599.0 24 4428.0 25 5648.5 26 7715.0 27 10509.5 28 13997.5 29 18532.5 30 23789.5 31 29493.5 32 35090.5 33 39812.0 34 44305.0 35 48355.0 36 52196.0 37 55634.5 38 57267.0 39 58741.5 40 61082.0 41 62939.0 42 64273.5 43 66895.5 44 69319.5 45 71068.0 46 72513.5 47 73164.5 48 74881.5 49 74937.5 50 72393.5 51 69445.5 52 66275.5 53 63485.0 54 60706.0 55 57124.0 56 51726.5 57 45396.5 58 40391.5 59 35742.0 60 30085.0 61 24614.5 62 19624.0 63 14558.0 64 10263.5 65 7270.0 66 5210.5 67 3977.5 68 3094.5 69 2247.0 70 1574.5 71 1055.5 72 765.5 73 576.5 74 427.5 75 338.5 76 258.0 77 163.5 78 109.0 79 89.5 80 64.5 81 44.0 82 36.5 83 34.5 84 29.0 85 20.5 86 15.0 87 14.0 88 12.0 89 9.0 90 5.0 91 4.0 92 4.0 93 4.0 94 3.0 95 1.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14981503385278577 2 0.15408208240295196 3 0.14783762111002582 4 0.15210466966019204 5 0.14065649062316082 6 0.12020587988882778 7 0.1269707129561644 8 0.11677142617771842 9 0.11375326988613745 10-11 0.10090008705819786 12-13 0.11945134081593253 14-15 0.13826278046087248 16-17 0.14950281078813948 18-19 0.1458602083672659 20-21 0.13657157219403832 22-23 0.13032711090111218 24-25 0.11544447815297161 26-27 0.11955541517081464 28-29 0.12387450089842186 30-31 0.11388336282974007 32-33 0.11357113976509378 34-35 0.12871395840043962 36-37 0.1305352596108764 38-39 0.11757800242805469 40-41 0.12004976835650462 42-43 0.11495012496728163 44-45 0.13100359420784585 46-47 0.1294164602958938 48-49 0.14297214501928757 50-51 0.12980673912670168 52-53 0.11458586472519426 54-55 0.10740473423832923 56-57 0.12639830400431284 58-59 0.11643318452435159 60-61 0.11575670121761791 62-63 0.11684948194387998 64-65 0.13693583243612567 66-67 0.12821960521474962 68-69 0.13368350884605998 70-71 0.12041402859859199 72-73 0.12262560863983665 74-75 0.10459472665651248 76-77 0.130613315377038 78-79 0.13108164997400742 80-81 0.13128979868377164 82-83 0.13802861316238774 84-85 0.1278813635613828 86-87 0.13732611126693353 88-89 0.12416070537434765 90-91 0.13162804033713846 92-93 0.14580817118982486 94-95 0.12223532980902878 96-97 0.10727464129472661 98-99 0.11026677899758704 100-101 0.1554350490164193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1921703.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.37689706361868 #Duplication Level Percentage of deduplicated Percentage of total 1 62.03378037285579 32.49136929056196 2 19.95925769578583 20.90807971596827 3 8.311798589837501 13.060386574603491 4 3.9647169970497482 8.306382961634162 5 2.092290265744205 5.47938359380478 6 1.1402807756415934 3.5834621285641797 7 0.7248821510877403 2.6576954467543943 8 0.4552896817194253 1.9077328634836843 9 0.2844400354759978 1.3408277813008547 >10 1.0052149719564738 8.170767274493139 >50 0.022174964381585734 0.7627532810787221 >100 0.005077525460701046 0.4583143458386453 >500 1.9899325086328415E-4 0.056855550386758956 >1k 5.969797525898376E-4 0.8159891915269023 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4913 0.25565865276788347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4458 0.23198173703220532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2007 0.10443861512418931 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.2037177441050985E-5 0.0 10-11 0.0 0.0 0.0 5.2037177441050985E-5 0.0 12-13 0.0 0.0 0.0 5.2037177441050985E-5 0.0 14-15 0.0 0.0 0.0 5.2037177441050985E-5 0.0 16-17 0.0 0.0 0.0 5.2037177441050985E-5 0.0 18-19 0.0 0.0 0.0 5.2037177441050985E-5 0.0 20-21 0.0 0.0 0.0 5.2037177441050985E-5 0.0 22-23 0.0 0.0 0.0 1.5611153232315296E-4 0.0 24-25 0.0 0.0 0.0 4.943531856899843E-4 0.0 26-27 0.0 0.0 0.0 8.586134277773413E-4 0.0 28-29 0.0 0.0 0.0 0.0017432454442752078 0.0 30-31 0.0 0.0 0.0 0.004553253026091961 0.0 32-33 0.0 0.0 0.0 0.009366691939389177 0.0 34-35 0.0 0.0 0.0 0.01558513464359477 0.0 36-37 0.0 0.0 0.0 0.0225581164206956 0.0 38-39 0.0 0.0 0.0 0.02880257771362172 0.0 40-41 0.0 0.0 0.0 0.03650407997489727 0.0 42-43 0.0 0.0 0.0 0.04904503973819055 0.0 44-45 0.0 0.0 0.0 0.06775240502824839 0.0 46-47 0.0 0.0 0.0 0.09184561818345499 0.0 48-49 0.0 0.0 0.0 0.11177585714337751 0.0 50-51 0.0 0.0 0.0 0.1330070255393263 0.0 52-53 0.0 0.0 0.0 0.1571782944606945 0.0 54-55 0.0 0.0 0.0 0.18663133689232936 0.0 56-57 0.0 0.0 0.0 0.22243291497177242 0.0 58-59 0.0 0.0 0.0 0.260289961560137 0.0 60-61 0.0 0.0 0.0 0.29728839472072427 0.0 62-63 0.0 0.0 0.0 0.3409215680050455 0.0 64-65 0.0 0.0 0.0 0.3941035633497997 0.0 66-67 0.0 0.0 0.0 0.4587077191428644 0.0 68-69 0.0 0.0 0.0 0.544543043331878 0.0 70-71 0.0 0.0 0.0 0.6242379805828475 0.0 72-73 0.0 0.0 0.0 0.714080167434822 0.0 74-75 0.0 0.0 0.0 0.8099586668699585 0.0 76-77 0.0 0.0 0.0 0.9166348806241131 0.0 78-79 0.0 0.0 0.0 1.0350194593025042 0.0 80-81 0.0 0.0 0.0 1.1525714431418383 0.0 82-83 0.0 0.0 0.0 1.2801145650498542 0.0 84-85 0.0 0.0 0.0 1.425766624707356 0.0 86-87 0.0 0.0 0.0 1.6096660097840303 0.0 88-89 0.0 0.0 0.0 1.8269472441891386 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4130 0.0 46.929806 1 GTATCAA 6640 0.0 35.199432 1 TCAACGC 7985 0.0 29.210157 4 CAACGCA 8015 0.0 28.982285 5 ATCAACG 8000 0.0 28.917868 3 TATCAAC 8015 0.0 28.864502 2 AACGCAG 8370 0.0 27.805456 6 ACGCAGA 9160 0.0 25.14813 7 CGCAGAG 9260 0.0 24.468767 8 GAGTACT 5260 0.0 22.984797 12-13 GCAGAGT 10020 0.0 21.901754 9 GTACATG 5395 0.0 21.044813 1 CAGAGTA 9320 0.0 20.412802 10-11 GTACTTT 5885 0.0 20.019588 14-15 TACATGG 5520 0.0 19.879778 2 AGAGTAC 8030 0.0 19.610289 10-11 GTGGTAT 2275 0.0 19.210783 1 ACATGGG 5810 0.0 18.55996 3 CATGGGG 3525 0.0 17.51909 4 TGGTATC 2500 0.0 17.101772 2 >>END_MODULE