FastQCFastQC Report
Fri 10 Feb 2017
SRR2031732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921703
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63170.32871884989511907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60000.3122230646463059No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT29870.1554350490164193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27060.14081260215548397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.045.4307941
GTATCAA70100.033.277591
TCAACGC83700.027.5256064
ATCAACG83600.027.5024263
TATCAAC84450.027.3943652
CAACGCA84950.027.1170525
AACGCAG87150.026.4325126
ACGCAGA97700.023.4810057
CGCAGAG100900.022.6421688
GTGGTAT24650.022.1650811
GAGTACT60900.021.95515412-13
GCAGAGT108800.020.4305979
GTACATG49850.019.8237861
TGGTATC26650.019.7859862
CAGAGTA103700.019.44349710-11
AGAGTAC87150.019.37526510-11
GTACTTT68500.019.17255414-15
TACATGG50650.018.8516142
GTACGAC1559.622026E-418.388723
AGTACTT66400.017.41832212-13
Produced by FastQC (version 0.11.5)