##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031732_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921703 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.801122754140465 34.0 31.0 34.0 31.0 34.0 2 32.960119227580954 34.0 31.0 34.0 31.0 34.0 3 33.01270903984643 34.0 31.0 34.0 31.0 34.0 4 36.38996348551259 37.0 37.0 37.0 35.0 37.0 5 36.36275168431334 37.0 37.0 37.0 35.0 37.0 6 36.391425209826906 37.0 37.0 37.0 35.0 37.0 7 36.34190246879981 37.0 37.0 37.0 35.0 37.0 8 36.334991411263864 37.0 37.0 37.0 35.0 37.0 9 38.19214415547044 39.0 39.0 39.0 37.0 39.0 10-11 38.162137437470825 39.0 39.0 39.0 37.0 39.0 12-13 38.10935248578995 39.0 39.0 39.0 37.0 39.0 14-15 39.61317461647299 41.0 40.0 41.0 37.0 41.0 16-17 39.587296788317445 41.0 40.0 41.0 37.0 41.0 18-19 39.59950236847213 41.0 40.0 41.0 37.0 41.0 20-21 39.595419531530105 41.0 40.0 41.0 37.0 41.0 22-23 39.50803063740859 41.0 39.0 41.0 36.5 41.0 24-25 39.444442767690944 41.0 39.0 41.0 36.0 41.0 26-27 39.3181792399762 41.0 39.0 41.0 36.0 41.0 28-29 39.21878562920493 41.0 39.0 41.0 36.0 41.0 30-31 39.053580860309836 40.0 39.0 41.0 35.5 41.0 32-33 38.847651015791726 40.0 38.0 41.0 35.0 41.0 34-35 38.70521173146943 40.0 38.0 41.0 35.0 41.0 36-37 38.60400748710909 40.0 38.0 41.0 35.0 41.0 38-39 38.67909323136822 40.0 38.0 41.0 35.0 41.0 40-41 38.59945605538421 40.0 38.0 41.0 35.0 41.0 42-43 38.494720828348605 40.0 38.0 41.0 35.0 41.0 44-45 38.30323936633288 40.0 38.0 41.0 34.0 41.0 46-47 38.07301778682762 40.0 38.0 41.0 34.0 41.0 48-49 37.808168327780095 40.0 38.0 41.0 33.0 41.0 50-51 37.64618439998272 40.0 38.0 41.0 33.0 41.0 52-53 37.69533013165926 40.0 38.0 41.0 33.0 41.0 54-55 37.61615114302262 40.0 37.0 41.0 33.0 41.0 56-57 37.43741254501866 40.0 37.0 41.0 33.0 41.0 58-59 37.256205823688674 40.0 36.5 41.0 33.0 41.0 60-61 36.99330905972463 39.5 36.0 41.0 32.5 41.0 62-63 36.7178406340626 39.0 35.5 41.0 32.0 41.0 64-65 36.399111881492615 39.0 35.0 41.0 32.0 41.0 66-67 36.053549117631604 38.5 35.0 40.5 31.0 41.0 68-69 35.661848110764254 37.0 35.0 40.0 31.0 41.0 70-71 35.30745671937859 37.0 35.0 39.5 31.0 41.0 72-73 34.91974384179033 36.5 35.0 39.0 30.0 41.0 74-75 34.53712384275822 36.0 35.0 39.0 30.0 40.5 76-77 33.62279993318427 35.0 33.5 37.0 28.5 39.0 78-79 33.81468546388281 35.0 34.0 37.0 29.0 39.0 80-81 33.588721045864006 35.0 34.0 37.0 29.0 39.0 82-83 33.31943463688197 35.0 34.0 36.0 29.0 38.0 84-85 33.031329242864274 35.0 34.0 36.0 29.0 37.0 86-87 32.79008749010643 35.0 34.0 35.5 29.0 37.0 88-89 32.572533841077416 35.0 34.0 35.0 29.0 36.5 90-91 32.38155687949698 35.0 34.0 35.0 29.0 36.0 92-93 32.218958132448144 35.0 34.0 35.0 28.5 36.0 94-95 32.04520157381239 35.0 34.0 35.0 27.0 36.0 96-97 31.887252348567912 35.0 34.0 35.0 27.0 35.5 98-99 31.69146507030483 35.0 33.5 35.0 26.5 35.0 100-101 31.001714364810795 34.5 32.0 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16130816549355842 1101 2 0.1494572191939767 1101 3 0.21511323254816261 1101 4 0.14872730125448896 1101 5 0.09920698510023129 1101 6 0.09307617995786188 1101 7 0.15959438229052836 1101 8 0.14928796616485585 1101 9 0.15352619390992572 1101 10-11 0.1294813359801239 1101 12-13 0.15873119761654664 1101 14-15 0.07440346794099639 1101 16-17 0.08942802398637895 1101 18-19 0.1007340758890578 1101 20-21 0.08621651269690034 1101 22-23 -0.04868895744051116 1101 24-25 -0.005146378711060606 1101 26-27 0.08885103609177492 1101 28-29 0.03653654406554807 1101 30-31 0.0558550384545029 1101 32-33 0.04197802405136031 1101 34-35 -0.3923020700219624 1101 36-37 -0.20573649923083792 1101 38-39 -0.2488026543623718 1101 40-41 -0.4553053409012975 1101 42-43 -0.3452894615200961 1101 44-45 -0.3110730074951604 1101 46-47 -0.32122990650670147 1101 48-49 -0.3856558568723969 1101 50-51 -0.21060908998604333 1101 52-53 -0.24473893061488639 1101 54-55 -0.24133616215238618 1101 56-57 -0.3194000243122872 1101 58-59 -0.31149051544177553 1101 60-61 -0.3305054385310129 1101 62-63 -0.23289209210127382 1101 64-65 -0.28418314226848906 1101 66-67 -0.3978714691425367 1101 68-69 -0.3263865451565522 1101 70-71 -0.339404405920547 1101 72-73 -0.4235813098695189 1101 74-75 -0.4133276990583994 1101 76-77 -0.42382173800308465 1101 78-79 -0.43704017563381825 1101 80-81 -0.46780703224214193 1101 82-83 -0.4197382437150097 1101 84-85 -0.2725984955435905 1101 86-87 -0.34354600403754887 1101 88-89 -0.3566490488198042 1101 90-91 -0.3728084395205258 1101 92-93 -0.5730832314106102 1101 94-95 -0.37853432273039544 1101 96-97 -0.34271447500698926 1101 98-99 -0.5544811312786173 1101 100-101 -0.8215971617242417 1102 1 0.1527552518858286 1102 2 0.09042073878367063 1102 3 0.12360328535508103 1102 4 0.07224012428115145 1102 5 0.08693740261513483 1102 6 0.08919380462698001 1102 7 0.1415519868134325 1102 8 0.05935319145450535 1102 9 0.019971406012260218 1102 10-11 0.13461727486481578 1102 12-13 0.08892783465982035 1102 14-15 0.16944476960171784 1102 16-17 0.23997060689246297 1102 18-19 0.16657124911380805 1102 20-21 0.2164879291026054 1102 22-23 0.2573297239436627 1102 24-25 0.03034209969231938 1102 26-27 0.1785972283996955 1102 28-29 0.22284692263366424 1102 30-31 0.09328009088657296 1102 32-33 0.265129830001797 1102 34-35 0.21804112688229083 1102 36-37 0.2510927404610541 1102 38-39 0.07819769358273021 1102 40-41 0.20951567882777056 1102 42-43 0.20368390818406823 1102 44-45 0.20157239415075878 1102 46-47 0.20417816005269884 1102 48-49 0.2879658672052159 1102 50-51 0.2885564184655536 1102 52-53 0.3866512927043573 1102 54-55 0.5235659806896535 1102 56-57 0.5124288789685849 1102 58-59 0.3739378895663208 1102 60-61 0.34798271115074186 1102 62-63 0.298145764710668 1102 64-65 0.34070262216306446 1102 66-67 0.2775093961834827 1102 68-69 0.23502615041839192 1102 70-71 0.18986008426297474 1102 72-73 0.172083601692826 1102 74-75 0.23216199042462193 1102 76-77 0.2704696554497801 1102 78-79 0.31441994428229236 1102 80-81 0.13820607433094523 1102 82-83 0.27657584498599874 1102 84-85 0.39987904661831664 1102 86-87 0.388687553633865 1102 88-89 0.2522606526971245 1102 90-91 0.13415546069419548 1102 92-93 0.24985577054442132 1102 94-95 0.2281906295473064 1102 96-97 -0.05975090543623196 1102 98-99 0.004332741158172837 1102 100-101 0.015220044065102911 1103 1 0.11044084404767318 1103 2 0.080867231797356 1103 3 0.05608801832796928 1103 4 0.05935553133704019 1103 5 0.03251309811303571 1103 6 0.05571871375953208 1103 7 0.04991849054537312 1103 8 0.054953521645835224 1103 9 0.04392972583973176 1103 10-11 0.0969575275216954 1103 12-13 -0.01181995794198798 1103 14-15 0.0670687102018448 1103 16-17 0.12852292968474188 1103 18-19 0.1591143922413849 1103 20-21 0.09102872977336318 1103 22-23 0.06203062851231067 1103 24-25 -0.08501471439353736 1103 26-27 0.055635533187029296 1103 28-29 0.09680102863200091 1103 30-31 0.0816207497997894 1103 32-33 -0.02211501724149656 1103 34-35 -0.07674323311270115 1103 36-37 -0.052966365515672464 1103 38-39 -0.15242463495764014 1103 40-41 -0.23522103248387083 1103 42-43 -0.17142413871692952 1103 44-45 -0.09496286587491198 1103 46-47 -0.08905194527142157 1103 48-49 -1.9467752404267458E-4 1103 50-51 -0.10058530017166589 1103 52-53 -0.131407126094075 1103 54-55 0.12954648470684305 1103 56-57 -0.0038516280739102626 1103 58-59 -0.06291193820692342 1103 60-61 -0.16450156802516602 1103 62-63 -0.21483607044434194 1103 64-65 -0.11386149502892806 1103 66-67 -0.04024735566736126 1103 68-69 -0.055756152363599654 1103 70-71 -0.053891023217758516 1103 72-73 0.00795063485458769 1103 74-75 0.1261922910975457 1103 76-77 0.12194028181927763 1103 78-79 0.1476981736961207 1103 80-81 0.06806580784406435 1103 82-83 0.12100181341037342 1103 84-85 -0.021687547008639285 1103 86-87 -0.03261648602366307 1103 88-89 0.09764877877488942 1103 90-91 -0.024702116202782065 1103 92-93 -0.028911176611998712 1103 94-95 -0.009597369467307715 1103 96-97 -0.06611108626352191 1103 98-99 -0.015548428860107322 1103 100-101 -0.11652262279172731 1104 1 0.09854020072512526 1104 2 0.03257273669435534 1104 3 0.06490384356828827 1104 4 0.048204469978408326 1104 5 0.09956567593364696 1104 6 0.05276980889054528 1104 7 0.07513862876498933 1104 8 0.122036988833095 1104 9 0.06654897938788906 1104 10-11 0.156361898161137 1104 12-13 0.1792276858171178 1104 14-15 0.14357034527899515 1104 16-17 0.12673443535603468 1104 18-19 0.12371208913020126 1104 20-21 0.16321614997583112 1104 22-23 0.14639360740046214 1104 24-25 0.12175561698758486 1104 26-27 0.1668331345247509 1104 28-29 0.1965170523879749 1104 30-31 0.2750746752205018 1104 32-33 0.30390165477932385 1104 34-35 0.1801430363578973 1104 36-37 0.16908699483555978 1104 38-39 0.18495623505166492 1104 40-41 0.18769249376384778 1104 42-43 0.17212224887460081 1104 44-45 0.166697870033353 1104 46-47 0.15879918445568109 1104 48-49 0.1886515840388796 1104 50-51 0.34500411012972876 1104 52-53 0.22625364276284898 1104 54-55 0.17549647688153414 1104 56-57 0.12678774062980125 1104 58-59 0.10080994217297956 1104 60-61 0.278899845472246 1104 62-63 0.19216222446262776 1104 64-65 0.11060741379390038 1104 66-67 0.06327835664143322 1104 68-69 0.1337212819244229 1104 70-71 0.2243778954896669 1104 72-73 0.11943447765654014 1104 74-75 0.22903225946682682 1104 76-77 0.11847758381843221 1104 78-79 0.29313766760473214 1104 80-81 0.3324983495858973 1104 82-83 0.2507937838224805 1104 84-85 0.32218729751487984 1104 86-87 0.37229574698924495 1104 88-89 0.33631855908971175 1104 90-91 0.315143725264754 1104 92-93 0.33232864019345953 1104 94-95 0.35719223687859625 1104 96-97 0.3347268392889795 1104 98-99 0.36525877206904056 1104 100-101 0.4668313034527003 1105 1 0.2045571582883028 1105 2 0.0861304184275582 1105 3 0.12551130629088192 1105 4 0.08595127601789443 1105 5 0.08348656925013387 1105 6 -0.0041950769905767515 1105 7 0.08123417800818089 1105 8 0.09730743272855591 1105 9 0.05736340914221927 1105 10-11 0.10324646227237366 1105 12-13 0.1946727614984951 1105 14-15 0.18512938426099623 1105 16-17 0.23094418662542893 1105 18-19 0.11468578103472282 1105 20-21 0.121961553167246 1105 22-23 0.2523870947520308 1105 24-25 0.2233444161979108 1105 26-27 0.12885129108045135 1105 28-29 0.18318362517379683 1105 30-31 0.19382449081169995 1105 32-33 0.22828968577610453 1105 34-35 0.1176240925442471 1105 36-37 0.0258449405080583 1105 38-39 -0.00935699973360471 1105 40-41 0.02816010084836762 1105 42-43 0.058916030207342374 1105 44-45 0.25700386794651564 1105 46-47 0.22767218512894516 1105 48-49 0.2409086807602847 1105 50-51 0.20363330224481047 1105 52-53 0.2881593434760745 1105 54-55 0.2913062125935397 1105 56-57 0.18241549502678112 1105 58-59 0.24650228659230322 1105 60-61 0.1344108523309231 1105 62-63 0.0898991596016927 1105 64-65 0.2260426319864024 1105 66-67 0.24883753778586737 1105 68-69 0.24151493450627726 1105 70-71 0.2147225857231092 1105 72-73 0.1085858752741089 1105 74-75 0.00145435848161668 1105 76-77 0.033631762991369385 1105 78-79 0.11676808017082152 1105 80-81 0.06458454293215965 1105 82-83 0.11975221606599007 1105 84-85 0.13489635004255973 1105 86-87 0.17023868352847416 1105 88-89 0.016231813187609134 1105 90-91 0.034162040076736844 1105 92-93 0.13587324566590553 1105 94-95 0.08681271685113501 1105 96-97 0.0030712907968428738 1105 98-99 0.05152239485043708 1105 100-101 0.12128373671012582 1106 1 0.03329228994876132 1106 2 0.02054772466934196 1106 3 0.01879427713351589 1106 4 0.08370660710655642 1106 5 0.0944623781474192 1106 6 0.06737188853327325 1106 7 0.09104488450864778 1106 8 0.03169493101135856 1106 9 0.0387956426914684 1106 10-11 0.08202092747870893 1106 12-13 0.051565074414675394 1106 14-15 0.1482737869743076 1106 16-17 0.09780499382496544 1106 18-19 0.1106361106070679 1106 20-21 0.13503558317737685 1106 22-23 0.11524002746639184 1106 24-25 0.0974631374105499 1106 26-27 0.08618929778334916 1106 28-29 0.13615735788194172 1106 30-31 0.17162242136173944 1106 32-33 0.19965946517127975 1106 34-35 0.23502695099791282 1106 36-37 0.2288112212264508 1106 38-39 0.11941014308280984 1106 40-41 0.23322525067872135 1106 42-43 0.20105657862194448 1106 44-45 0.278067078963538 1106 46-47 0.1805321939935709 1106 48-49 0.20832372159719625 1106 50-51 0.33511329332834094 1106 52-53 0.3883322296381877 1106 54-55 0.29754741600564216 1106 56-57 0.2825585732299061 1106 58-59 0.2616039058124713 1106 60-61 0.3897137530341297 1106 62-63 0.44400410435328297 1106 64-65 0.3819894494035765 1106 66-67 0.31655052861278676 1106 68-69 0.24634285186306215 1106 70-71 0.18771236288917237 1106 72-73 0.24387966510985848 1106 74-75 0.25765765394587703 1106 76-77 0.2867055467618371 1106 78-79 0.17173881653108225 1106 80-81 0.27685285085096467 1106 82-83 0.23351882752313458 1106 84-85 0.22351865209827082 1106 86-87 0.25205051495539266 1106 88-89 0.17142435964267122 1106 90-91 0.14934031759143096 1106 92-93 0.055075856843117776 1106 94-95 0.35647171380035303 1106 96-97 0.3475975127287647 1106 98-99 0.31539994375492597 1106 100-101 0.4073866830324704 1107 1 0.21448619867189223 1107 2 0.19921061312666666 1107 3 0.2124186845829925 1107 4 0.12464535098766305 1107 5 0.12131494248487229 1107 6 0.11426294066414755 1107 7 0.1351199221346704 1107 8 0.14190084613464649 1107 9 0.2018476324262437 1107 10-11 0.1912907722382755 1107 12-13 0.1913764625130412 1107 14-15 0.3171098026462644 1107 16-17 0.25403747855401804 1107 18-19 0.2868972440427271 1107 20-21 0.2618135740222769 1107 22-23 0.27611011487820036 1107 24-25 0.31288485424299495 1107 26-27 0.3717514896333256 1107 28-29 0.3290658869452514 1107 30-31 0.3520590596782682 1107 32-33 0.3706051167698945 1107 34-35 0.34799256033595327 1107 36-37 0.36500453960437795 1107 38-39 0.3977999799168188 1107 40-41 0.3337074337888808 1107 42-43 0.40857784801342234 1107 44-45 0.5080594655460118 1107 46-47 0.550843529842993 1107 48-49 0.44943563340640225 1107 50-51 0.45105539607012446 1107 52-53 0.462347608470175 1107 54-55 0.3651816381138744 1107 56-57 0.4410794938427287 1107 58-59 0.38212423743719626 1107 60-61 0.25540139663389994 1107 62-63 0.38376650058484785 1107 64-65 0.3777477851457931 1107 66-67 0.46137883315491734 1107 68-69 0.37462181236693226 1107 70-71 0.4740250395686374 1107 72-73 0.40956174453639704 1107 74-75 0.5059497241847524 1107 76-77 0.5211862533304128 1107 78-79 0.46636043239413993 1107 80-81 0.4913748001572813 1107 82-83 0.47815482880967863 1107 84-85 0.4385342521400659 1107 86-87 0.46682251277858455 1107 88-89 0.49675003341655355 1107 90-91 0.46157050608118766 1107 92-93 0.6099423370421277 1107 94-95 0.5943275664284684 1107 96-97 0.6064961185640847 1107 98-99 0.7996974397412586 1107 100-101 0.8253083421875047 1108 1 -0.012195089758478161 1108 2 -0.060385723285385495 1108 3 -0.05437297457983448 1108 4 0.044993829209381886 1108 5 0.016792057923908033 1108 6 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 5.0 5 16.0 6 26.0 7 91.0 8 246.0 9 480.0 10 892.0 11 1505.0 12 2272.0 13 3490.0 14 4396.0 15 5249.0 16 7159.0 17 10943.0 18 11128.0 19 6580.0 20 4719.0 21 4565.0 22 5085.0 23 5636.0 24 6465.0 25 7747.0 26 8144.0 27 9702.0 28 12039.0 29 15752.0 30 20653.0 31 26374.0 32 34132.0 33 46409.0 34 66319.0 35 105825.0 36 191803.0 37 424068.0 38 657822.0 39 213728.0 40 233.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.963465043631054 19.705798771481124 14.44597462809771 23.884761556790114 2 16.16644195278875 21.638411346602467 38.6972388553278 23.49790784528098 3 18.583140098830604 25.513302998638686 31.030633739784935 24.872923162745774 4 13.703621005799668 18.95944292378613 36.873405065379636 30.463531005034568 5 13.913440318301006 36.7437111770133 34.8408677095264 14.501980795159295 6 30.002190765170266 36.457350589555205 18.599284072512766 14.941174572761765 7 25.594329612848604 32.92517105921154 23.173820304178115 18.306679023761735 8 24.517628374415818 33.88910773412957 21.790932313682188 19.80233157777242 9 25.383787192922114 16.410704463697044 21.599591612231443 36.6059167311494 10-11 23.79574263036484 26.272738295147587 29.165771193571537 20.76574788091604 12-13 24.870368852618967 24.73712762511367 28.493978646538682 21.898524875728683 14-15 22.4109871325036 26.407313306820384 26.691332295191373 24.490367265484643 16-17 21.566834185236598 28.739031393144792 27.660572598680062 22.03356182293855 18-19 21.419186137274288 27.49752823021283 29.74644845709528 21.3368371754176 20-21 22.36040116500833 26.619956361622997 29.449061587560614 21.57058088580806 22-23 22.15454209105153 26.80533880625674 29.094428223299857 21.945690879391872 24-25 21.961484162745233 26.99488422508577 29.351465861269926 21.692165750899072 26-27 21.78900490921031 27.086986793943264 29.50628455935232 21.617723737494106 28-29 21.682527655468828 26.91478051260648 29.691281859667328 21.711409972257364 30-31 21.87944999754124 26.723102793593483 29.62092688220667 21.776520326658602 32-33 21.824036009783004 26.95342665348389 29.211999791850968 22.010537544882137 34-35 22.02000946140263 26.94155215923468 29.294594666329427 21.743843713033264 36-37 21.90703750914916 27.010636052985376 29.387218991015153 21.695107446850308 38-39 21.796318161547337 26.98507521713813 29.41953048936282 21.79907613195171 40-41 22.00446687131154 26.92713182005752 29.29138893991423 21.777012368716704 42-43 22.006236135344537 26.973835186810863 29.057247660018227 21.962681017826377 44-45 22.05192477713778 27.14084850780792 28.899366863662074 21.907859851392228 46-47 22.131723788743628 27.11568853251517 28.6648873421127 22.0877003366285 48-49 22.08715394626537 27.218201772074043 28.589459453411898 22.105184828248696 50-51 22.03995622632634 27.419273425706265 28.452419546620888 22.088350801346515 52-53 22.197030446432148 27.457104453705906 28.34873547056959 21.997129629292353 54-55 22.12019755394044 27.421511024336226 28.284365482075014 22.173925939648324 56-57 22.221045603821192 27.59149046444742 28.053788748833718 22.13367518289767 58-59 22.102987206606667 27.601466413764932 28.33051030470496 21.965036074923443 60-61 22.16595395357369 27.668758187961114 28.22515575827513 21.94013210019007 62-63 21.984664572988216 27.73593109752623 28.307047858741498 21.972356470744057 64-65 22.064485006681736 27.733241537507237 28.110702435815778 22.091571019995254 66-67 22.010234692260596 27.738841794201345 28.02560780682155 22.225315706716504 68-69 21.950318348697852 27.67570324229107 28.123290843474436 22.25068756553664 70-71 22.04304054822307 27.58698745563497 28.06315451505295 22.306817481089006 72-73 22.026463470070716 27.703950109143406 27.992180289447944 22.277406131337933 74-75 21.998884772777007 27.70246799316084 27.961317279542918 22.337329954519234 76-77 21.983323862394542 27.697682430055554 27.843285104398884 22.475708603151013 78-79 22.0249919797429 27.671226960136526 27.836523613380155 22.46725744674042 80-81 22.07276630346398 27.721190437469506 27.75025532606928 22.455787932997236 82-83 22.10613637079216 27.658151612904064 27.801085443621805 22.43462657268197 84-85 22.104020542564964 27.606764642288717 27.738863388768664 22.550351426377652 86-87 22.017354171800005 27.663873697389853 27.76521781439638 22.553554316413766 88-89 22.0038705393569 27.57129639651641 27.7154919404526 22.709341123674086 90-91 21.95287200987874 27.56513363407353 27.786916084327284 22.695078271720448 92-93 22.094855448526644 27.457234546649506 27.799717229977784 22.64819277484606 94-95 21.992940636508347 27.424893440869898 27.796673055097482 22.785492867524272 96-97 22.022821436414176 27.39886309115565 27.772334107993853 22.805981364436317 98-99 21.973969936009816 27.39291908574747 27.68052886479218 22.952582113450532 100-101 21.79983337574394 27.476974682167615 27.689339950429027 23.03385199165942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2193.0 1 1293.5 2 430.5 3 458.5 4 368.0 5 335.0 6 522.0 7 992.5 8 1625.5 9 1905.0 10 1556.0 11 1190.5 12 1227.0 13 1344.0 14 1459.5 15 1582.5 16 1682.5 17 1797.5 18 1965.0 19 2144.0 20 2382.0 21 2754.5 22 3281.0 23 3973.0 24 4921.5 25 6351.5 26 8451.5 27 11163.0 28 14570.0 29 19141.0 30 24503.0 31 30014.5 32 35500.5 33 40397.0 34 44821.0 35 48868.5 36 52893.5 37 56303.5 38 58099.5 39 60045.5 40 62675.5 41 64744.5 42 66134.0 43 69455.5 44 72246.5 45 73444.0 46 74349.5 47 74681.5 48 76113.5 49 75573.5 50 72412.5 51 69045.5 52 65528.0 53 62511.0 54 59180.5 55 54290.5 56 48204.5 57 42305.5 58 37528.5 59 32689.5 60 27450.5 61 22791.0 62 18373.5 63 13617.5 64 9505.5 65 6590.5 66 4677.5 67 3549.5 68 2757.5 69 1913.5 70 1265.5 71 846.5 72 585.5 73 418.5 74 297.0 75 204.5 76 139.5 77 86.5 78 44.0 79 21.5 80 18.5 81 10.5 82 4.0 83 3.0 84 2.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018993569765983608 2 0.0 3 0.0016131525006725805 4 0.02008635049224568 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.6018588720525496E-5 14-15 2.6018588720525496E-5 16-17 0.0013269480247468002 18-19 1.5611153232315293E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.683345969694589E-4 28-29 0.00582816387339771 30-31 1.3009294360262746E-4 32-33 1.5611153232315293E-4 34-35 0.011031881617502808 36-37 0.004084918429122502 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.1629741952840793E-4 60-61 0.0028880633479783295 62-63 0.010641602786694926 64-65 0.0024457473397293964 66-67 0.005281773510266674 68-69 0.003538528065991467 70-71 1.8213012104367844E-4 72-73 0.007727520849996072 74-75 0.0063225170590876945 76-77 0.0018213012104367845 78-79 3.3824165336683137E-4 80-81 0.007311223430467663 82-83 0.009600859237873908 84-85 0.0017952826217162588 86-87 0.001691208266834157 88-89 3.642602420873569E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 5.203717744105099E-5 98-99 1.0407435488210198E-4 100-101 7.285204841747137E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1921703.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.669962489739085 #Duplication Level Percentage of deduplicated Percentage of total 1 60.20907140084917 30.507913894230498 2 20.798998429328748 21.077689404764595 3 8.846320496002456 13.447281831139634 4 4.063080494301085 8.23504544956106 5 2.202128971554352 5.579089619311336 6 1.2193855955702282 3.707173343284297 7 0.7471134689590839 2.6499348013414936 8 0.4804436252583712 1.9475248376220742 9 0.3283340898208316 1.4973008413791744 >10 1.0730612444414638 8.536387685444852 >50 0.02394574049087008 0.7826575765972701 >100 0.006986820495459509 0.6731197149852578 >500 3.080789803307557E-4 0.11437183376388575 >1k 6.161579606615113E-4 0.6036002145864763 >5k 2.0538598688717044E-4 0.6409089519880714 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6317 0.32871884989511907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6000 0.3122230646463059 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2987 0.1554350490164193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.14081260215548397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.5611153232315296E-4 0.0 6 0.0 0.0 0.0 1.5611153232315296E-4 0.0 7 0.0 0.0 0.0 1.5611153232315296E-4 0.0 8 0.0 0.0 0.0 1.5611153232315296E-4 0.0 9 0.0 0.0 0.0 2.0814870976420394E-4 0.0 10-11 0.0 0.0 0.0 2.0814870976420394E-4 0.0 12-13 0.0 0.0 0.0 2.3416729848472941E-4 0.0 14-15 0.0 0.0 0.0 2.601858872052549E-4 0.0 16-17 0.0 0.0 0.0 2.601858872052549E-4 0.0 18-19 0.0 0.0 0.0 3.122230646463059E-4 0.0 20-21 0.0 0.0 0.0 3.382416533668314E-4 0.0 22-23 0.0 0.0 0.0 4.162974195284079E-4 0.0 24-25 0.0 0.0 0.0 7.025018954541884E-4 0.0 26-27 0.0 0.0 0.0 0.0010927807262620707 0.0 28-29 0.0 0.0 0.0 0.002055468508921514 0.0 30-31 0.0 0.0 0.0 0.00473538314713564 0.0 32-33 0.0 0.0 0.0 0.009600859237873908 0.0 34-35 0.0 0.0 0.0 0.0159233762969616 0.0 36-37 0.0 0.0 0.0 0.023312655493590843 0.0 38-39 0.0 0.0 0.0 0.029583135375237483 0.0 40-41 0.0 0.0 0.0 0.03751880493499776 0.0 42-43 0.0 0.0 0.0 0.0502158762306142 0.0 44-45 0.0 0.0 0.0 0.06884518575451046 0.0 46-47 0.0 0.0 0.0 0.09280830596611443 0.0 48-49 0.0 0.0 0.0 0.11245234045011118 0.0 50-51 0.0 0.0 0.0 0.1336054530798984 0.0 52-53 0.0 0.0 0.0 0.15795885212231026 0.0 54-55 0.0 0.0 0.0 0.1876980990298709 0.0 56-57 0.0 0.0 0.0 0.2239419931175629 0.0 58-59 0.0 0.0 0.0 0.26203320700441224 0.0 60-61 0.0 0.0 0.0 0.29918775169732265 0.0 62-63 0.0 0.0 0.0 0.3430030551026876 0.0 64-65 0.0 0.0 0.0 0.39636718056848536 0.0 66-67 0.0 0.0 0.0 0.46133559660363754 0.0 68-69 0.0 0.0 0.0 0.5469107349054458 0.0 70-71 0.0 0.0 0.0 0.6266056721564155 0.0 72-73 0.0 0.0 0.0 0.7173064724361673 0.0 74-75 0.0 0.0 0.0 0.813445157758509 0.0 76-77 0.0 0.0 0.0 0.9203555388111482 0.0 78-79 0.0 0.0 0.0 1.03978086103836 0.0 80-81 0.0 0.0 0.0 1.1580873839505896 0.0 82-83 0.0 0.0 0.0 1.2863330077540598 0.0 84-85 0.0 0.0 0.0 1.4322712718874873 0.0 86-87 0.0 0.0 0.0 1.6174195492227468 0.0 88-89 0.0 0.0 0.0 1.835143099636104 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4455 0.0 45.430794 1 GTATCAA 7010 0.0 33.27759 1 TCAACGC 8370 0.0 27.525606 4 ATCAACG 8360 0.0 27.502426 3 TATCAAC 8445 0.0 27.394365 2 CAACGCA 8495 0.0 27.117052 5 AACGCAG 8715 0.0 26.432512 6 ACGCAGA 9770 0.0 23.481005 7 CGCAGAG 10090 0.0 22.642168 8 GTGGTAT 2465 0.0 22.165081 1 GAGTACT 6090 0.0 21.955154 12-13 GCAGAGT 10880 0.0 20.430597 9 GTACATG 4985 0.0 19.823786 1 TGGTATC 2665 0.0 19.785986 2 CAGAGTA 10370 0.0 19.443497 10-11 AGAGTAC 8715 0.0 19.375265 10-11 GTACTTT 6850 0.0 19.172554 14-15 TACATGG 5065 0.0 18.851614 2 GTACGAC 155 9.622026E-4 18.38872 3 AGTACTT 6640 0.0 17.418322 12-13 >>END_MODULE