FastQCFastQC Report
Fri 10 Feb 2017
SRR2031731_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031731_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891646
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.29204415205137463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23580.26445472754882543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11200.12561038797908586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.12527393158271333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.049.456631
AATAGCG459.6182467E-442.2272645
GTATCAA28550.038.272921
ATAGCGT500.001616455537.9960026
TAGACGC550.00257709434.551524
TCAACGC32800.033.0240944
ATCAACG33000.032.967913
TATCAAC33350.032.7643742
CAACGCA33550.032.425635
AACGCAG35000.031.2110026
ACGCAGA38600.028.4231727
CGCAGAG38900.027.8408168
GTGGTAT7350.026.5012511
GCAGAGT43050.024.1639359
TGGTATC8500.022.3568672
CAGAGTA40800.021.3665110-11
GAGTACT26750.021.22454512-13
AGAGTAC37250.020.15062310-11
GTACTTT29950.018.87749914-15
AGTACTT29000.018.10333312-13
Produced by FastQC (version 0.11.5)