##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031731_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 891646 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07761712607918 33.0 31.0 34.0 30.0 34.0 2 32.38089107112015 34.0 31.0 34.0 31.0 34.0 3 32.49297703348638 34.0 31.0 34.0 31.0 34.0 4 35.905175372289 37.0 37.0 37.0 35.0 37.0 5 35.911482808199665 37.0 37.0 37.0 35.0 37.0 6 35.93930326609439 37.0 37.0 37.0 35.0 37.0 7 35.92293578393219 37.0 37.0 37.0 35.0 37.0 8 35.913249204280625 37.0 37.0 37.0 35.0 37.0 9 37.707922202308986 39.0 39.0 39.0 35.0 39.0 10-11 37.63875461786404 39.0 38.0 39.0 35.0 39.0 12-13 37.590424338807104 39.0 38.0 39.0 35.0 39.0 14-15 39.01398593163654 41.0 39.0 41.0 36.0 41.0 16-17 38.940359739179 41.0 39.0 41.0 36.0 41.0 18-19 38.95068109989839 41.0 39.0 41.0 36.0 41.0 20-21 38.95313611007059 41.0 39.0 41.0 36.0 41.0 22-23 38.84929332941549 41.0 39.0 41.0 35.5 41.0 24-25 38.783174039921676 41.0 39.0 41.0 35.0 41.0 26-27 38.664976347115335 41.0 39.0 41.0 35.0 41.0 28-29 38.54653416266096 40.0 38.5 41.0 35.0 41.0 30-31 38.37145627300521 40.0 38.0 41.0 34.5 41.0 32-33 38.1714475251389 40.0 38.0 41.0 34.0 41.0 34-35 37.99349854090076 40.0 38.0 41.0 34.0 41.0 36-37 37.822360555646526 40.0 38.0 41.0 33.0 41.0 38-39 37.62859027012962 40.0 38.0 41.0 33.0 41.0 40-41 37.52533236284355 40.0 38.0 41.0 33.0 41.0 42-43 37.474640159884075 40.0 38.0 41.0 33.0 41.0 44-45 37.313422591476886 40.0 37.5 41.0 32.5 41.0 46-47 37.20631842681961 40.0 37.5 41.0 32.0 41.0 48-49 37.21612557001321 40.0 38.0 41.0 32.0 41.0 50-51 36.64758940207213 39.5 36.5 40.5 31.0 41.0 52-53 36.65894536621036 39.5 37.0 40.5 31.0 41.0 54-55 36.86890144743542 40.0 37.0 41.0 31.0 41.0 56-57 36.725624855604124 40.0 36.5 41.0 31.0 41.0 58-59 36.5336820890802 40.0 36.0 41.0 31.0 41.0 60-61 36.293082120034185 39.0 35.5 41.0 31.0 41.0 62-63 36.00866487372791 39.0 35.0 41.0 30.0 41.0 64-65 35.72263151519773 39.0 35.0 41.0 29.5 41.0 66-67 35.4244464731519 38.0 35.0 41.0 29.0 41.0 68-69 35.085804231724254 37.0 35.0 40.0 29.0 41.0 70-71 34.72613234400199 37.0 35.0 39.5 29.0 41.0 72-73 34.35901131166405 36.0 35.0 39.0 28.0 41.0 74-75 33.95746742541323 36.0 34.0 39.0 28.0 41.0 76-77 33.583143422389604 35.0 34.0 37.5 27.0 40.0 78-79 33.20383481785372 35.0 34.0 37.0 26.5 39.0 80-81 32.85844438263616 35.0 34.0 37.0 26.0 39.0 82-83 32.53829939235975 35.0 34.0 36.0 26.0 38.0 84-85 32.221495414099316 35.0 34.0 36.0 25.0 37.0 86-87 31.963564015315498 35.0 34.0 35.5 25.0 37.0 88-89 31.693775332362843 35.0 33.0 35.0 24.0 36.5 90-91 31.464698434131932 35.0 33.0 35.0 23.5 36.0 92-93 31.253961774067285 35.0 33.0 35.0 22.0 36.0 94-95 31.0134212456513 35.0 33.0 35.0 19.5 36.0 96-97 30.758132151100323 35.0 33.0 35.0 17.5 35.5 98-99 30.510066775379467 35.0 33.0 35.0 4.5 35.0 100-101 29.930490912312735 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10168314355053099 1101 2 0.2146168431497557 1101 3 0.3599977878819729 1101 4 0.43042539829983184 1101 5 0.34153052460896305 1101 6 0.40537235537325955 1101 7 0.41902450195649976 1101 8 0.40004086708870545 1101 9 0.46223155897784807 1101 10-11 0.3054816129954716 1101 12-13 0.36384208459001854 1101 14-15 0.3008696553646715 1101 16-17 0.3736867062888649 1101 18-19 0.33336835323318326 1101 20-21 0.32677040542914426 1101 22-23 0.31715050447711235 1101 24-25 0.27608908647012953 1101 26-27 0.1787960959207524 1101 28-29 0.11978071573712867 1101 30-31 0.17437466093203113 1101 32-33 0.07774951769149396 1101 34-35 0.14414962423187916 1101 36-37 0.015429668938111263 1101 38-39 0.03531225156289253 1101 40-41 -0.02125030734237754 1101 42-43 0.04266457833389836 1101 44-45 -0.1089371889576114 1101 46-47 0.007479028122446607 1101 48-49 0.41057936998335975 1101 50-51 0.22594468794317635 1101 52-53 0.3615246958187939 1101 54-55 0.4108201239990379 1101 56-57 0.2743541328549739 1101 58-59 0.1315743679123571 1101 60-61 -0.00476793290830102 1101 62-63 0.1206874357533394 1101 64-65 0.12994442707900333 1101 66-67 -0.10464938817697345 1101 68-69 -0.0577381221347224 1101 70-71 -0.17312067663663555 1101 72-73 0.11906768882892749 1101 74-75 0.32349381473289185 1101 76-77 -0.08775222094170942 1101 78-79 -0.1579077945269347 1101 80-81 -0.041346168964196295 1101 82-83 0.027832281191827235 1101 84-85 0.16136883154082682 1101 86-87 0.20716419868492153 1101 88-89 -0.10856307796064613 1101 90-91 -0.4472789474612462 1101 92-93 -0.1687534640915196 1101 94-95 -0.37346569926965856 1101 96-97 -0.4835364277160785 1101 98-99 -0.5515136764858823 1101 100-101 -0.6179249854999753 1102 1 -0.1041087597859196 1102 2 -0.028688783056324496 1102 3 0.11675353103076702 1102 4 0.2070341277496155 1102 5 0.17453769118584717 1102 6 0.17651239342226432 1102 7 0.06171166144181939 1102 8 -0.09776006436172224 1102 9 0.16746431930324235 1102 10-11 0.03876698731443895 1102 12-13 0.04681481613999949 1102 14-15 0.2125830195645122 1102 16-17 0.1881637371030962 1102 18-19 0.1207337668285362 1102 20-21 -0.014165340856301611 1102 22-23 0.1399021699732188 1102 24-25 -0.12526990379941338 1102 26-27 -0.19087073667046894 1102 28-29 -0.16296835860843117 1102 30-31 -0.2144053162931172 1102 32-33 -0.28121006717891106 1102 34-35 -0.06746384429587238 1102 36-37 0.009159766515381307 1102 38-39 -0.23328607237184684 1102 40-41 -0.07020430078267026 1102 42-43 0.1424017129305355 1102 44-45 -0.25301197774475526 1102 46-47 -0.13275165246369625 1102 48-49 -0.051439818168262263 1102 50-51 -0.04133249015751517 1102 52-53 -0.09627193977679127 1102 54-55 -0.05694391765029394 1102 56-57 -0.04602969836566473 1102 58-59 -0.061696482325963586 1102 60-61 -0.2982856243295444 1102 62-63 -0.06131872845742237 1102 64-65 -0.042511983631996486 1102 66-67 -0.17781529681845853 1102 68-69 0.09356461289286244 1102 70-71 -0.06128696031307612 1102 72-73 -0.05472521005483344 1102 74-75 -0.5952429311911587 1102 76-77 -0.18103875445960682 1102 78-79 -0.3829253529812462 1102 80-81 -0.14627753756899864 1102 82-83 0.08684403001387153 1102 84-85 0.15775997253350482 1102 86-87 0.13637805724901852 1102 88-89 -0.05775436623978436 1102 90-91 0.17449660435153902 1102 92-93 0.05912970229443815 1102 94-95 -0.1661020585667785 1102 96-97 -0.5436664625602319 1102 98-99 -0.34079688242097816 1102 100-101 -0.3386858973122102 1103 1 -0.07308086019420656 1103 2 0.1424104406948743 1103 3 0.07409450083770963 1103 4 -0.013380837722429817 1103 5 0.09116970388810586 1103 6 0.022034471147954093 1103 7 0.09471168664470042 1103 8 0.12451361374705527 1103 9 -0.025737833022979828 1103 10-11 -0.06600769890966518 1103 12-13 0.07768362253145256 1103 14-15 0.3224896428822248 1103 16-17 0.1911455532228672 1103 18-19 0.12367462826308895 1103 20-21 0.05663270844053869 1103 22-23 0.03581111195618547 1103 24-25 -0.05570994614026148 1103 26-27 0.053885488441679286 1103 28-29 -0.03497236586685659 1103 30-31 -0.06092920862640483 1103 32-33 -0.10999428009287016 1103 34-35 0.05579243122205213 1103 36-37 0.06401856736219713 1103 38-39 0.09724501538459407 1103 40-41 0.16755969941895188 1103 42-43 0.1428443385669027 1103 44-45 0.01598154511426486 1103 46-47 0.04316289716077648 1103 48-49 0.039462256029025866 1103 50-51 0.2780085174537632 1103 52-53 0.29947003310490317 1103 54-55 0.20028773465072902 1103 56-57 0.18759787862914123 1103 58-59 0.30326489425551983 1103 60-61 0.4068374917549349 1103 62-63 0.406316043960544 1103 64-65 0.3280853877072829 1103 66-67 0.37956064198893813 1103 68-69 0.2765265187348831 1103 70-71 0.4515428824209309 1103 72-73 0.48711945197450035 1103 74-75 0.44797371279290843 1103 76-77 0.5199495135982559 1103 78-79 0.4429936071477911 1103 80-81 0.3733051026850447 1103 82-83 0.47572294276148597 1103 84-85 0.4745287108771734 1103 86-87 0.5350624667454582 1103 88-89 0.624883052480925 1103 90-91 0.645499112038415 1103 92-93 0.22397388546898966 1103 94-95 0.7352144770448987 1103 96-97 0.7602629819682214 1103 98-99 0.7534871036670303 1103 100-101 0.834810750704321 1104 1 0.05815277044216316 1104 2 0.24006475512823044 1104 3 0.30590681059916136 1104 4 0.10789845016601163 1104 5 0.16704834054132078 1104 6 0.1528610797697425 1104 7 0.18119944059104398 1104 8 0.08910712263075027 1104 9 0.03998294987501083 1104 10-11 0.19814074024939288 1104 12-13 0.12177251260011701 1104 14-15 0.2922505616901745 1104 16-17 0.4512569533785964 1104 18-19 0.3518657693404492 1104 20-21 0.34403891641108686 1104 22-23 0.37189184388794416 1104 24-25 0.332005067663097 1104 26-27 0.44776113436792286 1104 28-29 0.2783062849436462 1104 30-31 0.41190207190025063 1104 32-33 0.4241778725226837 1104 34-35 0.2902879893731054 1104 36-37 0.3472284668454577 1104 38-39 0.4015802422551289 1104 40-41 0.5116348746153889 1104 42-43 0.4888816253227617 1104 44-45 0.331348395825664 1104 46-47 0.4361714092298641 1104 48-49 0.4516583167637833 1104 50-51 0.4528045640312328 1104 52-53 0.5523457321209762 1104 54-55 0.5462055692936119 1104 56-57 0.3958736979821609 1104 58-59 0.56937102082167 1104 60-61 0.520153305445028 1104 62-63 0.5132986835867897 1104 64-65 0.5762604248701635 1104 66-67 0.5647711431569249 1104 68-69 0.5001209655388763 1104 70-71 0.6438674205394292 1104 72-73 0.5813251396647345 1104 74-75 0.6206837715456572 1104 76-77 0.5457755028387226 1104 78-79 0.46278505260684 1104 80-81 0.5966894022532543 1104 82-83 0.4792290834849311 1104 84-85 0.4963220852666481 1104 86-87 0.46967349527239577 1104 88-89 0.8010461621291114 1104 90-91 0.7067315923951796 1104 92-93 0.4868857740511423 1104 94-95 0.7548599254769783 1104 96-97 0.6437374457609586 1104 98-99 0.5634432160758926 1104 100-101 0.4767198609188057 1105 1 -0.07918434070621672 1105 2 0.16435840080801256 1105 3 0.087401107098799 1105 4 0.0639269191211369 1105 5 0.15963835051545772 1105 6 0.33633225627667684 1105 7 0.1765978029494164 1105 8 0.271143965154117 1105 9 0.1913956585154395 1105 10-11 0.21133661289818662 1105 12-13 0.08341106632070705 1105 14-15 0.14622579993673668 1105 16-17 0.2562478471966898 1105 18-19 0.18404575103982523 1105 20-21 0.16174021599123023 1105 22-23 0.20297216953325403 1105 24-25 0.19436196526488914 1105 26-27 0.17500377071791462 1105 28-29 0.01695665757625875 1105 30-31 0.22866700883368907 1105 32-33 0.27735650374144427 1105 34-35 0.2690142419512327 1105 36-37 0.2745886450265118 1105 38-39 0.3088428268808343 1105 40-41 0.42447517954743574 1105 42-43 0.15911279582756066 1105 44-45 0.4571909750965304 1105 46-47 0.5725581821696437 1105 48-49 0.5248387721611465 1105 50-51 0.2660697260447691 1105 52-53 0.36750421499281316 1105 54-55 0.3817396858468527 1105 56-57 0.506396119196765 1105 58-59 0.35206427342038893 1105 60-61 0.46146918550822846 1105 62-63 0.5991689491813048 1105 64-65 0.5328096673947087 1105 66-67 0.45774481908276954 1105 68-69 0.6375382522791568 1105 70-71 0.4426303626452963 1105 72-73 0.2216809721816091 1105 74-75 0.2618729888847682 1105 76-77 0.39202961091835675 1105 78-79 0.48369396430777556 1105 80-81 0.3227154291628267 1105 82-83 0.5136876702172586 1105 84-85 0.48942036436007896 1105 86-87 0.43920456368203986 1105 88-89 0.41883440441533537 1105 90-91 0.3006554594184436 1105 92-93 0.31223464820929436 1105 94-95 0.434656264762431 1105 96-97 0.41326498350233365 1105 98-99 0.45235809460619336 1105 100-101 0.498205448436714 1106 1 0.008626822879023166 1106 2 0.08491244149519162 1106 3 0.051910627288293654 1106 4 0.11509555283379314 1106 5 0.14406586235148922 1106 6 0.05542282649008712 1106 7 0.05732173066230928 1106 8 0.15515086864901662 1106 9 0.17981938986617507 1106 10-11 0.1121135495897434 1106 12-13 0.16870753209528289 1106 14-15 0.21407127553528227 1106 16-17 0.1460150010019774 1106 18-19 0.05946144298897593 1106 20-21 0.09362250435889763 1106 22-23 0.2819345613359303 1106 24-25 0.25371382336754067 1106 26-27 0.2044081975253178 1106 28-29 0.2589201911559442 1106 30-31 0.21855758609148523 1106 32-33 0.23724461546535025 1106 34-35 0.37185635679227147 1106 36-37 0.2460038614798492 1106 38-39 0.2857079005386609 1106 40-41 0.48638723043135457 1106 42-43 0.18049689959131854 1106 44-45 0.07478006452202379 1106 46-47 0.22639869147850789 1106 48-49 0.313859514932588 1106 50-51 0.27739577235571744 1106 52-53 0.3592439426687335 1106 54-55 0.4378452576599301 1106 56-57 0.16821512552790097 1106 58-59 0.3015266989774119 1106 60-61 0.20978367022632227 1106 62-63 0.27024911718983446 1106 64-65 0.34392922524229874 1106 66-67 0.31697310697493464 1106 68-69 0.47054162477730443 1106 70-71 0.4797916819407462 1106 72-73 0.22920378570800182 1106 74-75 0.07917619378530816 1106 76-77 0.1709974327659225 1106 78-79 0.31122840106536387 1106 80-81 0.4054179478231035 1106 82-83 0.27400475548657965 1106 84-85 0.22938065165349286 1106 86-87 0.018555864104406794 1106 88-89 -0.023563826270581956 1106 90-91 0.05342478230652503 1106 92-93 0.00209763496879134 1106 94-95 -0.07034524684465993 1106 96-97 -0.11810086356999605 1106 98-99 0.29400331014962333 1106 100-101 0.32050502841540407 1107 1 0.11568558468708545 1107 2 0.1833934791544749 1107 3 0.1617406775603314 1107 4 0.27001613289100845 1107 5 0.18482046753546655 1107 6 0.2973350155302299 1107 7 0.19410240694310232 1107 8 0.2377155140444458 1107 9 0.3178499234163539 1107 10-11 0.22192894104194494 1107 12-13 0.3025988778366582 1107 14-15 0.27813571879330823 1107 16-17 0.4490891869745681 1107 18-19 0.4497826100162001 1107 20-21 0.5057575383210704 1107 22-23 0.329490858919236 1107 24-25 0.39208228129055556 1107 26-27 0.2763529137151366 1107 28-29 0.3081113329082754 1107 30-31 0.3087309304935104 1107 32-33 0.392311783720821 1107 34-35 0.2789371839817605 1107 36-37 0.39604922666962494 1107 38-39 0.5642717682451277 1107 40-41 0.34689081489856477 1107 42-43 0.26450968208220615 1107 44-45 0.13452015593134092 1107 46-47 0.32161852748488684 1107 48-49 0.35665786820705137 1107 50-51 0.13026134017668056 1107 52-53 0.14601597796099242 1107 54-55 0.14999695638569222 1107 56-57 0.3845615612744382 1107 58-59 0.35089991870231785 1107 60-61 0.39515910574757385 1107 62-63 0.47210029467882464 1107 64-65 0.4632720780646622 1107 66-67 0.3620304615362997 1107 68-69 0.48495278770005257 1107 70-71 0.5685330911008109 1107 72-73 0.5153262038269091 1107 74-75 0.31735346983712276 1107 76-77 0.316390551452983 1107 78-79 0.38764019971797836 1107 80-81 0.4122057529194336 1107 82-83 0.2610085544354632 1107 84-85 0.19953375145382068 1107 86-87 0.2604140469662326 1107 88-89 0.3554847083103425 1107 90-91 0.41871254265488034 1107 92-93 0.5796952549936663 1107 94-95 0.40330668179311857 1107 96-97 0.5116034603189483 1107 98-99 0.5255270250340338 1107 100-101 0.39139122351041067 1108 1 0.09294059348309247 1108 2 0.21326366924672158 1108 3 0.21209961833002922 1108 4 0.25205315436369347 1108 5 0.18165564460035455 1108 6 0.26599544336096415 1108 7 0.1639235596689943 1108 8 0.42890907542665246 1108 9 0.37430665489620196 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2316 44-45 -0.13607673328692016 2316 46-47 -0.06953764712879718 2316 48-49 -0.24671800949035827 2316 50-51 -0.22856709183187007 2316 52-53 -0.25928011366038817 2316 54-55 -0.25374275479055797 2316 56-57 -0.10677031775120582 2316 58-59 -0.21875566546537328 2316 60-61 -0.5379357768339403 2316 62-63 -0.38686282938520833 2316 64-65 -0.3603429027705829 2316 66-67 -0.026986033567503398 2316 68-69 -0.251881476199614 2316 70-71 -0.27366059717449787 2316 72-73 -0.24424187998342717 2316 74-75 -0.03569969669063511 2316 76-77 -0.1532256696486911 2316 78-79 -0.012142863608175958 2316 80-81 0.025156861352591875 2316 82-83 0.09097825147180316 2316 84-85 0.27415424971547253 2316 86-87 0.1416266493047118 2316 88-89 0.1268628114503727 2316 90-91 0.0997403145074287 2316 92-93 0.06856254428581465 2316 94-95 0.1800878605300298 2316 96-97 0.02542121592048474 2316 98-99 -0.0690988169287543 2316 100-101 -0.12461074244383497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5453.0 3 663.0 4 522.0 5 557.0 6 582.0 7 714.0 8 819.0 9 1025.0 10 1211.0 11 1476.0 12 1826.0 13 2405.0 14 2664.0 15 3266.0 16 3800.0 17 5388.0 18 5235.0 19 3059.0 20 2719.0 21 2719.0 22 2918.0 23 3178.0 24 3555.0 25 4240.0 26 4907.0 27 5789.0 28 6915.0 29 8286.0 30 10543.0 31 13389.0 32 17387.0 33 23331.0 34 34004.0 35 54807.0 36 95903.0 37 189880.0 38 271362.0 39 95008.0 40 141.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.64244567066918 19.761399079245738 13.68967728675127 23.90647796333381 2 16.35086473943252 21.78572792437826 38.60585897749813 23.257548358691086 3 18.729931253839666 25.667105057745204 30.80582719454793 24.797136493867207 4 13.622849826189018 18.63804073747522 37.38581545148737 30.3532939848484 5 14.071263164751146 36.586946316789565 34.858374342745094 14.483416175714192 6 29.72845821665405 36.7732293755572 18.83310240754173 14.66521000024702 7 25.462401523522065 32.81220524429353 23.649513568868407 18.075879663315998 8 24.35811645296746 34.129858756427815 22.01693126445557 19.495093526149148 9 25.503208769889206 16.500913904370123 21.857808156428092 36.13806916931257 10-11 23.820864860010328 26.301387547991652 29.439311614540063 20.438435977457956 12-13 24.814317874325624 24.9725196067974 28.51729384091126 21.69586867796572 14-15 22.4447683657186 26.560034678043344 26.70091422911541 24.294282727122653 16-17 21.667001730727545 28.826216310618985 27.682206525999657 21.82457543265381 18-19 21.593332019323803 27.762887541561167 29.442910497419994 21.20086994169504 20-21 22.41000886590031 27.109160625520428 29.297729734343037 21.183100774236223 22-23 22.280894126128313 27.12517215571804 28.771241896118728 21.82269182203492 24-25 21.94598915651423 27.255513527411296 29.193598548513318 21.60489876756116 26-27 21.84322835556284 27.382996138701348 29.32419975455659 21.449575751179218 28-29 21.835070472747077 27.360717724436263 29.27955265736013 21.52465914545654 30-31 22.083083757962317 27.191318538262077 29.109853768948312 21.615743934827286 32-33 21.919540758987164 27.364888002182585 28.948384786125764 21.767186452704486 34-35 22.089977093065038 27.453961552281708 28.86846478620194 21.58759656845131 36-37 22.120927986704547 27.333105004884732 28.946077055236767 21.599889953173953 38-39 21.95438247246797 27.454219949710573 29.027967949052623 21.56342962876883 40-41 22.124412980808366 27.37023823224767 28.842579207239883 21.662769579704083 42-43 22.167618765325646 27.371376860409764 28.69909415896455 21.761910215300045 44-45 22.165907285288775 27.511727599653014 28.552739808708882 21.769625306349326 46-47 22.19169160457922 27.5194964881056 28.372232733887675 21.916579173427507 48-49 22.11559572548163 27.657634235677996 28.416809901178013 21.809960137662358 50-51 22.14608872584193 27.849195039869024 28.156536307705093 21.848179926583953 52-53 22.156865496944707 27.867077220174757 27.993555395251313 21.982501887629223 54-55 22.21534285268432 27.864814588205178 27.969670400237106 21.950172158873396 56-57 22.08430274339304 27.970268680045272 27.93770548498949 22.007723091572196 58-59 22.0054285738345 27.918307542461605 28.157119880537696 21.9191440031662 60-61 22.126725536537307 27.844700308196686 28.140209843207042 21.888364312058968 62-63 22.028153200566994 27.91607370917716 28.100150169115683 21.955622921140165 64-65 22.102082171751796 27.77295832568304 28.07481511552367 22.050144387041495 66-67 22.018790571700954 27.82507048990908 28.079238405799543 22.076900532590425 68-69 21.923553746850114 27.971562299271874 27.972404515175608 22.132479438702404 70-71 21.980207135671172 27.77973645444093 28.087666624748493 22.15238978513941 72-73 22.043250107650874 27.81406686761497 27.97468702648352 22.16799599825064 74-75 21.974580033937446 27.96925983700371 27.819387228720753 22.236772900338085 76-77 22.01165697871908 27.94890063832093 27.779284355157746 22.260158027802248 78-79 21.89685491369204 27.87547218965742 27.88271505122781 22.344957845422737 80-81 21.957939323296536 27.896528219933682 27.887488588166732 22.258043868603057 82-83 22.010811126465025 27.96496013628735 27.81447806021866 22.209750677028968 84-85 22.046558423730726 27.92814845964639 27.6485486354911 22.376744481131784 86-87 21.89675076306646 27.958272224380842 27.83367734023893 22.31129967231377 88-89 21.929975752070394 27.851096714936084 27.791641847019648 22.42728568597387 90-91 21.921122385901004 27.79940967837746 27.931128769729092 22.348339165992446 92-93 22.01981120950579 27.688972995434664 27.92369764320731 22.36751815185225 94-95 21.942342241185717 27.784302717269256 27.909050078598696 22.364304962946328 96-97 21.874068531390808 27.785901571799776 27.886380897476847 22.45364899933257 98-99 22.00442236194995 27.622640831570205 27.865988623560895 22.50694818291895 100-101 22.000443656968923 27.695258205547958 27.82768138690538 22.476616750577737 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2132.0 1 1226.0 2 309.5 3 285.0 4 235.5 5 240.5 6 315.5 7 502.5 8 791.5 9 894.0 10 768.5 11 650.5 12 623.5 13 681.5 14 737.5 15 796.0 16 864.0 17 931.0 18 1034.0 19 1065.0 20 1201.5 21 1458.0 22 1772.5 23 2203.0 24 2760.5 25 3615.0 26 4806.5 27 6297.5 28 8290.5 29 10593.5 30 13102.0 31 15739.0 32 18124.0 33 20186.5 34 21971.0 35 23315.0 36 24830.5 37 26360.5 38 27454.5 39 28375.5 40 29147.0 41 29718.5 42 29945.5 43 30511.5 44 30926.0 45 31594.0 46 32440.0 47 32372.0 48 32166.5 49 32059.0 50 30868.0 51 29363.0 52 28343.0 53 27329.5 54 26019.0 55 24020.5 56 21874.0 57 19463.5 58 17131.5 59 15354.0 60 13371.0 61 11071.0 62 8824.5 63 6761.5 64 5210.5 65 3936.5 66 3062.5 67 2472.0 68 1923.0 69 1593.0 70 1306.5 71 1063.0 72 836.5 73 664.0 74 539.5 75 413.5 76 336.5 77 261.0 78 185.5 79 150.5 80 128.0 81 95.0 82 68.0 83 51.5 84 38.5 85 29.0 86 22.5 87 17.0 88 15.5 89 13.5 90 7.5 91 7.5 92 10.0 93 8.0 94 8.0 95 7.5 96 5.5 97 4.0 98 4.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14456409830807293 2 0.14804081440392264 3 0.14232105566558928 4 0.147031445214805 5 0.13458255854902057 6 0.11529239182366095 7 0.12089999842987015 8 0.11058200227444523 9 0.10789035110346482 10-11 0.09712374641954317 12-13 0.11338580557754983 14-15 0.13110584245317086 16-17 0.14232105566558928 18-19 0.13923687203217422 20-21 0.1295917886694944 22-23 0.12342342140266428 24-25 0.10912402455683086 26-27 0.11361010984179819 28-29 0.11815227119282765 30-31 0.10839503569802365 32-33 0.10755389470709228 34-35 0.12179721548686362 36-37 0.12516177945058915 38-39 0.1123203603223701 40-41 0.11478770722910213 42-43 0.1101333937459485 44-45 0.1254421597808996 46-47 0.12347949746872638 48-49 0.13531154740782778 50-51 0.12392810599722312 52-53 0.10974086128351386 54-55 0.10082476677964125 56-57 0.12000278137287669 58-59 0.1108623826047557 60-61 0.11002124161382432 62-63 0.11030162194413477 64-65 0.12981609293374274 66-67 0.1225822804117329 68-69 0.12762912635732118 70-71 0.11461947903091585 72-73 0.11613353281459234 74-75 0.0997593215244615 76-77 0.12409633419540939 78-79 0.12493747518634077 80-81 0.12594684437545842 82-83 0.1317226791798539 84-85 0.12196544368504991 86-87 0.13009647326405321 88-89 0.11826442332495182 90-91 0.12499355125240286 92-93 0.1391807959661121 94-95 0.11731113020189626 96-97 0.10267527695969028 98-99 0.1060398409234158 100-101 0.14787258620573637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 891646.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.70573750917924 #Duplication Level Percentage of deduplicated Percentage of total 1 71.73460878988412 47.13375375471788 2 18.058525469705046 23.730974686305352 3 5.803046986356677 11.438804461169564 4 2.185343037028011 5.743583038339002 5 0.9037744057475332 2.9691581935780937 6 0.47292983226210356 1.8644522051324373 7 0.26303755078702706 1.2098153386948864 8 0.15101374715776544 0.7937975704820559 9 0.1034634046613724 0.6118325377637513 >10 0.3162222175689663 3.1168268630841762 >50 0.004958323860765331 0.21556725721256828 >100 0.0020508233626868847 0.2257076887802132 >500 3.418038726213064E-4 0.13733357789087774 >1k 6.836077452426128E-4 0.8083928268490834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2604 0.29204415205137463 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2358 0.26445472754882543 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1120 0.12561038797908586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1117 0.12527393158271333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.607606606209191E-5 0.0 12-13 0.0 0.0 0.0 1.1215213212418382E-4 0.0 14-15 0.0 0.0 0.0 1.1215213212418382E-4 0.0 16-17 0.0 0.0 0.0 2.2430426424836764E-4 0.0 18-19 0.0 0.0 0.0 2.2430426424836764E-4 0.0 20-21 0.0 0.0 0.0 2.2430426424836764E-4 0.0 22-23 0.0 0.0 0.0 2.8038033031045954E-4 0.0 24-25 0.0 0.0 0.0 6.16836726683011E-4 0.0 26-27 0.0 0.0 0.0 0.0016822819818627572 0.0 28-29 0.0 0.0 0.0 0.0033645639637255144 0.0 30-31 1.1215213212418382E-4 0.0 0.0 0.007458116786258224 0.0 32-33 1.1215213212418382E-4 0.0 0.0 0.01682281981862757 0.0 34-35 1.1215213212418382E-4 0.0 0.0 0.02798195696498386 0.0 36-37 1.1215213212418382E-4 0.0 0.0 0.039477550507712705 0.0 38-39 1.1215213212418382E-4 0.0 0.0 0.049459090266765066 0.0 40-41 1.1215213212418382E-4 0.0 0.0 0.06532861696233708 5.607606606209191E-5 42-43 1.1215213212418382E-4 0.0 0.0 0.0831608059700823 1.1215213212418382E-4 44-45 1.1215213212418382E-4 0.0 0.0 0.11349795770967402 1.1215213212418382E-4 46-47 1.1215213212418382E-4 0.0 0.0 0.1489380314609161 1.1215213212418382E-4 48-49 1.1215213212418382E-4 0.0 0.0 0.18062100878599802 1.1215213212418382E-4 50-51 1.1215213212418382E-4 0.0 0.0 0.2162293107354264 1.1215213212418382E-4 52-53 1.1215213212418382E-4 0.0 0.0 0.25396850319521425 1.1215213212418382E-4 54-55 1.1215213212418382E-4 0.0 0.0 0.2979882150539564 1.1215213212418382E-4 56-57 1.1215213212418382E-4 0.0 0.0 0.3490735112365221 1.1215213212418382E-4 58-59 1.1215213212418382E-4 0.0 0.0 0.40144855693851594 1.1215213212418382E-4 60-61 1.1215213212418382E-4 0.0 0.0 0.45623487348117975 1.1215213212418382E-4 62-63 1.1215213212418382E-4 0.0 0.0 0.5203298169901509 1.1215213212418382E-4 64-65 1.1215213212418382E-4 0.0 0.0 0.5966493429006579 1.1215213212418382E-4 66-67 1.1215213212418382E-4 0.0 0.0 0.6873243417230606 1.1215213212418382E-4 68-69 1.1215213212418382E-4 0.0 0.0 0.792018357060986 1.1215213212418382E-4 70-71 1.1215213212418382E-4 0.0 0.0 0.8973292091255947 1.1215213212418382E-4 72-73 1.1215213212418382E-4 0.0 0.0 1.0210890869246316 1.1215213212418382E-4 74-75 1.1215213212418382E-4 0.0 0.0 1.1511855601886847 1.1215213212418382E-4 76-77 1.1215213212418382E-4 0.0 0.0 1.2949085175058264 1.1215213212418382E-4 78-79 1.1215213212418382E-4 0.0 0.0 1.449117699176579 1.1215213212418382E-4 80-81 1.1215213212418382E-4 0.0 0.0 1.6037754893758285 1.1215213212418382E-4 82-83 1.1215213212418382E-4 0.0 0.0 1.774246730204588 1.1215213212418382E-4 84-85 1.1215213212418382E-4 0.0 0.0 1.9718587870073998 1.1215213212418382E-4 86-87 1.1215213212418382E-4 0.0 0.0 2.2205561399927776 1.1215213212418382E-4 88-89 1.1215213212418382E-4 0.0 0.0 2.5002074814444297 1.1215213212418382E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1585 0.0 49.45663 1 AATAGCG 45 9.6182467E-4 42.227264 5 GTATCAA 2855 0.0 38.27292 1 ATAGCGT 50 0.0016164555 37.996002 6 TAGACGC 55 0.002577094 34.55152 4 TCAACGC 3280 0.0 33.024094 4 ATCAACG 3300 0.0 32.96791 3 TATCAAC 3335 0.0 32.764374 2 CAACGCA 3355 0.0 32.42563 5 AACGCAG 3500 0.0 31.211002 6 ACGCAGA 3860 0.0 28.423172 7 CGCAGAG 3890 0.0 27.840816 8 GTGGTAT 735 0.0 26.501251 1 GCAGAGT 4305 0.0 24.163935 9 TGGTATC 850 0.0 22.356867 2 CAGAGTA 4080 0.0 21.36651 10-11 GAGTACT 2675 0.0 21.224545 12-13 AGAGTAC 3725 0.0 20.150623 10-11 GTACTTT 2995 0.0 18.877499 14-15 AGTACTT 2900 0.0 18.103333 12-13 >>END_MODULE