FastQCFastQC Report
Fri 10 Feb 2017
SRR2031731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891646
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.3623635388932379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28600.3207550978751657No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT22170.24864127691931553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.1628448958443149No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.10575946059310534No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT9350.10486224353611186RNA PCR Primer, Index 31 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.050.3824161
GTATCAA27450.036.5270421
ATCAACG32850.030.517463
TATCAAC33050.030.3327852
TCAACGC33300.030.1050644
CAACGCA33350.029.7649825
AACGCAG34150.029.0677056
CCGTAGA350.00838952727.14502130-31
GTGGTAT8300.025.7637351
ACGCAGA39450.025.1625377
CGCAGAG39750.024.972638
TGGTATC8700.024.0289462
GCAGAGT44500.021.9868169
TACATGG19550.021.143352
GTACATG20300.021.0678831
GAGTACT29800.021.03958712-13
TACGTCT1150.003717288120.6572954
CAGAGTA42400.019.77235210-11
GTACTTT32900.018.9127614-15
AGAGTAC38350.018.76405710-11
Produced by FastQC (version 0.11.5)