FastQCFastQC Report
Fri 10 Feb 2017
SRR2031729_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031729_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485588
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89620.25711587255866153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77880.22343432442388486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37760.10833179366006539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.057.524241
GTATCAA95250.040.6489831
TCAACGC116550.032.8927044
ATCAACG118600.032.4443173
CAACGCA118600.032.3628165
TATCAAC121100.031.9328212
AACGCAG124950.030.825986
GTGGTAT25950.028.3760151
ACGCAGA139050.027.529777
TGGTATC26900.027.0206762
CGCAGAG141950.026.7362088
GCAGAGT158900.023.3162759
GAGTACT93450.022.11398512-13
CAGAGTA148150.021.77178210-11
GTACTTT102750.019.8127514-15
GTACATG87100.019.4173031
AGAGTAC140450.019.41404310-11
TACATGG88200.018.1517432
AGTACTT100800.017.83866712-13
ACATGGG89900.017.2264673
Produced by FastQC (version 0.11.5)