FastQCFastQC Report
Fri 10 Feb 2017
SRR2031729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485588
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106780.30634716438087345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95610.2743009213940374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46260.13271792305917968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57300.059.0399251
GTATCAA100750.039.1900981
TCAACGC118400.032.9405484
ATCAACG120750.032.2212523
CAACGCA121700.032.041825
TATCAAC123200.031.7732852
AACGCAG126950.030.641916
GTGGTAT27000.030.2680951
ACGCAGA141700.027.251187
TGGTATC29250.027.1229062
CGCAGAG145000.026.46728
GCAGAGT167150.022.363159
GAGTACT101200.022.24685912-13
CAGAGTA154950.021.42675810-11
GTACATG91250.020.7238581
TACATGG88000.020.4598372
GTACTTT110050.020.1772714-15
ACATGGG92350.019.0845833
AGAGTAC145600.018.43138310-11
TATATCG1300.007569043418.2680115
Produced by FastQC (version 0.11.5)