##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031729_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3485588 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79889533702779 34.0 31.0 34.0 31.0 34.0 2 32.955891516725444 34.0 31.0 34.0 31.0 34.0 3 33.0094147673219 34.0 31.0 34.0 31.0 34.0 4 36.389110244813786 37.0 37.0 37.0 35.0 37.0 5 36.36119300387768 37.0 37.0 37.0 35.0 37.0 6 36.389773834429086 37.0 37.0 37.0 35.0 37.0 7 36.33923114263648 37.0 37.0 37.0 35.0 37.0 8 36.33229859639177 37.0 37.0 37.0 35.0 37.0 9 38.19151230724916 39.0 39.0 39.0 37.0 39.0 10-11 38.15631781495691 39.0 39.0 39.0 37.0 39.0 12-13 38.105591223059065 39.0 39.0 39.0 37.0 39.0 14-15 39.61052095084101 41.0 40.0 41.0 37.0 41.0 16-17 39.5838943099414 41.0 40.0 41.0 37.0 41.0 18-19 39.59607302985895 41.0 40.0 41.0 37.0 41.0 20-21 39.59002699114181 41.0 40.0 41.0 37.0 41.0 22-23 39.50068309851881 41.0 39.0 41.0 37.0 41.0 24-25 39.43594194150313 41.0 39.0 41.0 36.0 41.0 26-27 39.309969652179205 41.0 39.0 41.0 36.0 41.0 28-29 39.21158467380539 41.0 39.0 41.0 36.0 41.0 30-31 39.0479534586417 40.0 39.0 41.0 35.5 41.0 32-33 38.84323520163599 40.0 38.0 41.0 35.0 41.0 34-35 38.70477735750754 40.0 38.0 41.0 35.0 41.0 36-37 38.60862127136082 40.0 38.0 41.0 35.0 41.0 38-39 38.690531697951684 40.0 38.0 41.0 35.0 41.0 40-41 38.610619356045525 40.0 38.0 41.0 35.0 41.0 42-43 38.507254299704954 40.0 38.0 41.0 35.0 41.0 44-45 38.32146111359116 40.0 38.0 41.0 34.0 41.0 46-47 38.10162905082299 40.0 38.0 41.0 34.0 41.0 48-49 37.847110587940975 40.0 38.0 41.0 33.0 41.0 50-51 37.691865188886354 40.0 38.0 41.0 33.0 41.0 52-53 37.74134694060228 40.0 38.0 41.0 33.0 41.0 54-55 37.65724807406957 40.0 37.0 41.0 33.0 41.0 56-57 37.481641691444885 40.0 37.0 41.0 33.0 41.0 58-59 37.29793954420316 40.0 36.5 41.0 33.0 41.0 60-61 37.037937358058386 39.5 36.0 41.0 32.5 41.0 62-63 36.76469336020207 39.0 35.5 41.0 32.0 41.0 64-65 36.44514368881234 39.0 35.0 41.0 32.0 41.0 66-67 36.10368465808351 38.0 35.0 40.5 31.0 41.0 68-69 35.72222089931455 37.0 35.0 40.0 31.0 41.0 70-71 35.3656763220438 37.0 35.0 39.5 31.0 41.0 72-73 34.97605152989969 36.0 35.0 39.0 30.5 41.0 74-75 34.592032678560976 36.0 35.0 39.0 30.0 40.5 76-77 33.67427016618143 35.0 33.5 37.0 29.0 39.0 78-79 33.86906470299989 35.0 34.0 37.0 30.0 39.0 80-81 33.65187423183693 35.0 34.0 37.0 30.0 39.0 82-83 33.387697427234656 35.0 34.0 36.0 29.5 38.0 84-85 33.10047357863293 35.0 34.0 36.0 29.0 37.0 86-87 32.86385453472987 35.0 34.0 35.5 29.0 37.0 88-89 32.6470106335 35.0 34.0 35.0 29.0 36.0 90-91 32.455726264836805 35.0 34.0 35.0 29.0 36.0 92-93 32.294190248531955 35.0 34.0 35.0 29.0 36.0 94-95 32.12143345685147 35.0 34.0 35.0 27.5 36.0 96-97 31.95983547108838 35.0 34.0 35.0 27.0 35.5 98-99 31.758045557880045 35.0 33.5 35.0 27.0 35.0 100-101 31.047514508312513 34.5 32.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1922152073201815 1101 2 0.1660575555902355 1101 3 0.20067409093250177 1101 4 0.13387348109086616 1101 5 0.07962222827557497 1101 6 0.10975629841789214 1101 7 0.14704778614348868 1101 8 0.16079133233982645 1101 9 0.14757687832011612 1101 10-11 0.14931213479163574 1101 12-13 0.20332985680249038 1101 14-15 0.1845961900550961 1101 16-17 0.08793783403903888 1101 18-19 0.12603103102447477 1101 20-21 0.14049784836686996 1101 22-23 0.01619707179235519 1101 24-25 0.11522924158393977 1101 26-27 0.16878362184584716 1101 28-29 0.13428061933031188 1101 30-31 0.1460023189468984 1101 32-33 0.21465306653989558 1101 34-35 -0.006234418618639381 1101 36-37 0.12027246657365254 1101 38-39 0.191131936968155 1101 40-41 -0.035361264086297695 1101 42-43 0.0659489249184162 1101 44-45 0.24052360214497526 1101 46-47 0.2947899195206034 1101 48-49 0.031951484709857425 1101 50-51 0.18415794454979562 1101 52-53 0.2850270557547603 1101 54-55 0.26777089127167386 1101 56-57 0.09086975388952823 1101 58-59 0.21974093664197625 1101 60-61 0.19577009381272603 1101 62-63 0.2401209136419169 1101 64-65 0.2855062990934485 1101 66-67 0.019403563881716934 1101 68-69 -0.0012281909489360032 1101 70-71 0.15247294791269184 1101 72-73 0.035401325645594284 1101 74-75 -0.08577054424071662 1101 76-77 -0.0924829886570464 1101 78-79 -0.04395803157570555 1101 80-81 -0.0013508853931298859 1101 82-83 0.010423976659488687 1101 84-85 0.0766192828181218 1101 86-87 0.1432180206564908 1101 88-89 0.011958809923477531 1101 90-91 -0.043023806450179336 1101 92-93 -0.12459474857363517 1101 94-95 0.046816158049793444 1101 96-97 0.07682663345383389 1101 98-99 -0.05909195182383442 1101 100-101 -0.324906731386605 1102 1 0.17324816195719706 1102 2 0.12149135535567268 1102 3 0.13397161373933386 1102 4 0.07463212709485845 1102 5 0.08967961464429663 1102 6 0.1084420520951852 1102 7 0.18025667789722633 1102 8 0.09956965622813385 1102 9 0.10379134756335162 1102 10-11 0.18082271232589875 1102 12-13 0.13245903432422068 1102 14-15 0.16583147416686472 1102 16-17 0.12938583267824555 1102 18-19 0.14124155215716172 1102 20-21 0.2293616698872043 1102 22-23 0.16816083153953088 1102 24-25 0.1779048250717068 1102 26-27 0.2317313653350439 1102 28-29 0.25842586682581015 1102 30-31 0.06903117327863129 1102 32-33 0.18247820373897383 1102 34-35 0.17324435916809477 1102 36-37 0.15770520992855808 1102 38-39 -0.048946309032253055 1102 40-41 0.07694233755740498 1102 42-43 0.16963561862203136 1102 44-45 0.1497206406144329 1102 46-47 0.15946910196627329 1102 48-49 0.223681043613702 1102 50-51 0.2241941131815608 1102 52-53 0.2076861664003289 1102 54-55 0.37225078813735735 1102 56-57 0.38308544069066386 1102 58-59 0.19524295546040804 1102 60-61 0.3241006965934119 1102 62-63 0.2668096037823844 1102 64-65 0.18768447436806213 1102 66-67 0.3107794938363284 1102 68-69 0.3306789881663619 1102 70-71 0.2314360541177578 1102 72-73 0.22022930082889758 1102 74-75 0.21706743998474565 1102 76-77 0.2627735490021976 1102 78-79 0.2799069985498974 1102 80-81 0.1052185556940799 1102 82-83 0.1420079657730895 1102 84-85 0.30233750769031786 1102 86-87 0.32092328211809473 1102 88-89 0.29838482985203285 1102 90-91 0.1755260462790318 1102 92-93 0.324062804314039 1102 94-95 0.2487141159545203 1102 96-97 0.06902978657068815 1102 98-99 0.13719628710400045 1102 100-101 0.24842337899934464 1103 1 0.17998060633928503 1103 2 0.15780539801264126 1103 3 0.15862739985652752 1103 4 0.1366378964252064 1103 5 0.08390761176464423 1103 6 0.1129803469858004 1103 7 0.1574212207354222 1103 8 0.11684691314038531 1103 9 0.12725551808431135 1103 10-11 0.16906974790232 1103 12-13 0.07624213823731196 1103 14-15 0.137026413725863 1103 16-17 0.17765945995677868 1103 18-19 0.21912417117256666 1103 20-21 0.25586718282143295 1103 22-23 0.1801950800483425 1103 24-25 0.09273855176846979 1103 26-27 0.1625703903282414 1103 28-29 0.20104391343812722 1103 30-31 0.14348025568116896 1103 32-33 0.05615342084050923 1103 34-35 0.08360159329700423 1103 36-37 0.11916795398509805 1103 38-39 -0.021004643743616214 1103 40-41 -0.1489982687400797 1103 42-43 0.0836916382517856 1103 44-45 0.16146842700556618 1103 46-47 0.056986652990779874 1103 48-49 0.05332477827795401 1103 50-51 -0.060487763537835804 1103 52-53 -0.05050172268287412 1103 54-55 0.1330241857451 1103 56-57 -0.015729290978683252 1103 58-59 0.10955152944697488 1103 60-61 0.020636425852465834 1103 62-63 -0.0778716382104605 1103 64-65 0.14976824825173907 1103 66-67 0.10453912957009237 1103 68-69 0.09501587429809177 1103 70-71 0.07357237667910965 1103 72-73 0.14923262980266117 1103 74-75 0.2864428857548731 1103 76-77 0.20190043251150058 1103 78-79 0.21689618982474457 1103 80-81 0.19930774813816043 1103 82-83 0.23708148548217878 1103 84-85 0.15547597997031204 1103 86-87 0.1958841545664498 1103 88-89 0.24170909327691703 1103 90-91 0.23373999255777989 1103 92-93 0.29199992629678206 1103 94-95 0.25097357546815147 1103 96-97 0.12795612989428662 1103 98-99 0.28209066359372414 1103 100-101 0.22238639448500308 1104 1 0.18053011661723417 1104 2 0.15413783399789338 1104 3 0.13546402542481673 1104 4 0.096186091320142 1104 5 0.10938570774143841 1104 6 0.06121526163280322 1104 7 0.13567133741585025 1104 8 0.15830959890570995 1104 9 0.0752467888769317 1104 10-11 0.09867634806953873 1104 12-13 0.13783398252686396 1104 14-15 0.19290196606920773 1104 16-17 0.07510202842049551 1104 18-19 0.1788426649372994 1104 20-21 0.25488552473370873 1104 22-23 0.19335992767545207 1104 24-25 0.15634082510623415 1104 26-27 0.18520896513131646 1104 28-29 0.16791983890703222 1104 30-31 0.15832595210386557 1104 32-33 0.2069878013597517 1104 34-35 0.12902359662449214 1104 36-37 0.16803166284475424 1104 38-39 0.2607314897522315 1104 40-41 0.2146493651654069 1104 42-43 0.31838341723386776 1104 44-45 0.3736864737751091 1104 46-47 0.3384395321434255 1104 48-49 0.3160682332611273 1104 50-51 0.4518088011887471 1104 52-53 0.4670510227489615 1104 54-55 0.370807314174165 1104 56-57 0.3614790855851737 1104 58-59 0.40356360922952916 1104 60-61 0.5568253029334116 1104 62-63 0.5055463199155383 1104 64-65 0.45182456250991976 1104 66-67 0.507248424048008 1104 68-69 0.49860174102385457 1104 70-71 0.4949623973961863 1104 72-73 0.4385015240582888 1104 74-75 0.48547536152899795 1104 76-77 0.5208240471202288 1104 78-79 0.5219496277843305 1104 80-81 0.4942970265494253 1104 82-83 0.4538684840920979 1104 84-85 0.4379173900239621 1104 86-87 0.4453580433322273 1104 88-89 0.39701290100435216 1104 90-91 0.4262344292515081 1104 92-93 0.4316119470675801 1104 94-95 0.5075414666917695 1104 96-97 0.44085606467076843 1104 98-99 0.48614496238629457 1104 100-101 0.5637235123775675 1105 1 0.10372986968230435 1105 2 0.06444036131767206 1105 3 0.04084687065330428 1105 4 0.013567379993801865 1105 5 0.07814851889226304 1105 6 0.03097113714685662 1105 7 0.06574267150504198 1105 8 0.07411151289164764 1105 9 -0.039147780155957435 1105 10-11 0.08788565585103214 1105 12-13 0.10407119539694065 1105 14-15 0.11432297091182164 1105 16-17 0.12865914453436034 1105 18-19 0.09153487223453283 1105 20-21 0.047005485947948955 1105 22-23 0.1700278194084106 1105 24-25 0.15538259105932894 1105 26-27 0.10324092014495534 1105 28-29 0.19767403696101837 1105 30-31 0.20476237036231737 1105 32-33 0.14015152706660672 1105 34-35 0.06239470283534132 1105 36-37 0.2524743617698988 1105 38-39 0.13585139411699032 1105 40-41 0.10040682846145188 1105 42-43 0.05420707814832326 1105 44-45 0.12498090044408627 1105 46-47 0.09070060017703696 1105 48-49 0.11603512444322206 1105 50-51 0.1987993817901028 1105 52-53 0.30071324234490504 1105 54-55 0.20483034782265008 1105 56-57 0.05132322592491789 1105 58-59 0.08502805588389606 1105 60-61 0.15970905294942384 1105 62-63 0.04047962586503928 1105 64-65 -0.00915337268487093 1105 66-67 0.20536446424939925 1105 68-69 0.1792757997245431 1105 70-71 0.24456184875865006 1105 72-73 0.17049234887220877 1105 74-75 0.1739610194456418 1105 76-77 0.07229744956972439 1105 78-79 0.22577263533009528 1105 80-81 0.1883406220949908 1105 82-83 0.3168912591154722 1105 84-85 0.3739842426124582 1105 86-87 0.27384756060806836 1105 88-89 0.24183953810929637 1105 90-91 0.24995589641653737 1105 92-93 0.23717250697892922 1105 94-95 0.1962212936131138 1105 96-97 0.36350944217053893 1105 98-99 0.341109823801677 1105 100-101 0.24482244088494198 1106 1 0.06179694897785737 1106 2 0.08475082092567021 1106 3 0.04851648615191806 1106 4 0.047937359800592105 1106 5 0.06751296507425053 1106 6 0.04200821031871271 1106 7 0.08194779273159725 1106 8 0.05675930334351875 1106 9 0.055773778454145884 1106 10-11 0.021032202919130327 1106 12-13 0.05752858648601489 1106 14-15 0.06880001742079145 1106 16-17 0.031362202039851184 1106 18-19 0.07246702757274193 1106 20-21 0.036454695191238784 1106 22-23 0.0940109221516181 1106 24-25 0.07672122083464927 1106 26-27 0.07810838416101973 1106 28-29 0.13260810436937476 1106 30-31 0.12285770785577199 1106 32-33 0.1967168394079124 1106 34-35 0.1444776368596976 1106 36-37 0.14653427791404994 1106 38-39 0.07026708062667097 1106 40-41 0.13848908438131957 1106 42-43 0.050186502828175605 1106 44-45 0.024611921648691748 1106 46-47 0.06138190298337065 1106 48-49 0.1410852999469583 1106 50-51 0.09858503311446043 1106 52-53 0.20269840861696764 1106 54-55 0.07736371390797103 1106 56-57 0.19237097808926507 1106 58-59 0.2052105444120471 1106 60-61 0.13032433282705114 1106 62-63 0.15618120453380158 1106 64-65 0.24305027470233398 1106 66-67 0.18575853752388838 1106 68-69 0.21027707251591465 1106 70-71 0.10101935248072635 1106 72-73 0.08556802633281535 1106 74-75 0.22513849561300248 1106 76-77 0.24412015138557308 1106 78-79 0.18799012542857696 1106 80-81 0.21005928253877926 1106 82-83 0.19662275818384956 1106 84-85 0.33148854131056993 1106 86-87 0.2588839971920365 1106 88-89 0.26777405566524237 1106 90-91 0.2639194094744681 1106 92-93 0.24531110630874764 1106 94-95 0.29607429409094976 1106 96-97 0.3024884951230433 1106 98-99 0.3541752520434933 1106 100-101 0.4291096373572607 1107 1 0.17493263054393537 1107 2 0.1318897855574832 1107 3 0.11770851767157353 1107 4 0.05423397697878585 1107 5 0.024339895356114027 1107 6 0.050404991809202215 1107 7 0.07960902081443066 1107 8 0.05599885961485995 1107 9 0.08982231845585176 1107 10-11 0.08983735421980299 1107 12-13 0.1103835001430582 1107 14-15 0.15899672310542456 1107 16-17 0.17013731919211494 1107 18-19 0.17322075331968279 1107 20-21 0.14718879767384863 1107 22-23 0.13801091976654334 1107 24-25 0.20558075598608383 1107 26-27 0.15350341837999082 1107 28-29 0.16038119479797786 1107 30-31 0.22949081045226194 1107 32-33 0.2486575212194495 1107 34-35 0.12516368788494958 1107 36-37 0.12129752371685498 1107 38-39 0.16084664170395513 1107 40-41 0.12012628633796396 1107 42-43 0.11372012543223065 1107 44-45 0.16483011294938166 1107 46-47 0.11064851006273813 1107 48-49 0.03946519306744989 1107 50-51 0.17754616283252744 1107 52-53 0.17191872323714819 1107 54-55 0.1530802373405109 1107 56-57 0.11158367038403583 1107 58-59 0.08501109389525396 1107 60-61 0.1377677273715392 1107 62-63 0.14696882428729197 1107 64-65 0.14278310573733677 1107 66-67 0.16743424862126233 1107 68-69 0.13187330670952946 1107 70-71 0.18473966541147746 1107 72-73 0.10017466763412841 1107 74-75 0.09129592734051784 1107 76-77 0.126700812383298 1107 78-79 0.15235595292392645 1107 80-81 0.23452903403109104 1107 82-83 0.20242427203531577 1107 84-85 0.2297252798059688 1107 86-87 0.2164147254987583 1107 88-89 0.22004781421501463 1107 90-91 0.1802706987008662 1107 92-93 0.23418658573513085 1107 94-95 0.22536122604633846 1107 96-97 0.293669752828599 1107 98-99 0.27708626283539317 1107 100-101 0.3652881651374038 1108 1 0.07415094543375034 1108 2 -0.007366649435205375 1108 3 -0.02314846691622563 1108 4 0.04784189798461114 1108 5 0.035233789468350096 1108 6 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-0.3337865981301782 2316 96-97 -0.4365052295462206 2316 98-99 -0.47198929842089044 2316 100-101 -0.2600953180890855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 6.0 4 15.0 5 23.0 6 41.0 7 135.0 8 372.0 9 885.0 10 1637.0 11 2729.0 12 4096.0 13 6099.0 14 7321.0 15 9124.0 16 12056.0 17 18268.0 18 18771.0 19 11313.0 20 8336.0 21 7997.0 22 8508.0 23 9102.0 24 10729.0 25 12666.0 26 14368.0 27 17394.0 28 22718.0 29 29125.0 30 37737.0 31 48443.0 32 63732.0 33 85339.0 34 121942.0 35 194319.0 36 357594.0 37 782787.0 38 1183779.0 39 375599.0 40 475.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.58891060459412 19.36154870122324 13.93668113562011 24.112859558562533 2 16.37580230365723 21.470982801180174 38.332815008543754 23.820399886618844 3 18.62922385669435 25.406731661105102 30.722194536324327 25.241849945876222 4 13.619939314530823 18.526084593500904 37.02226836475789 30.83170772721038 5 14.037717595998151 36.63149517384154 34.83676211875873 14.494025111401577 6 30.2919622169918 36.49636732740645 18.45565224576169 14.756018209840061 7 26.044242750434073 32.55106455496175 23.081069822365695 18.323622872238484 8 24.750400793209064 33.78959303279676 21.66463735817314 19.795368815821032 9 25.73428070098933 15.981005213467569 21.192866167774273 37.091847917768824 10-11 23.86258789047931 26.014405603875158 29.313146017257345 20.809860488388185 12-13 25.079857447212607 24.423525915011133 28.462574155393096 22.03404248238316 14-15 22.578645682339452 26.00096884727114 26.531912644231507 24.8884728261579 16-17 21.850385427032382 28.384132831853343 27.483500090875197 22.28198165023908 18-19 21.706848382852563 27.245033971371207 29.357808825039832 21.69030882073639 20-21 22.56385436259248 26.501956054473446 29.176052935688325 21.758136647245742 22-23 22.429300307437366 26.506718522097277 28.833599955014765 22.230381215450592 24-25 22.106772228961084 26.827998604539605 29.024213418223844 22.04101574827547 26-27 21.962834273923658 26.92601540577069 29.25538583712252 21.85576448318313 28-29 21.90940015983802 26.839876645559112 29.34261413348555 21.908109061117315 30-31 22.22334111682321 26.60955140072694 29.126590874467773 22.04051660798208 32-33 22.127728185383127 26.800420532413316 28.91772864872045 22.15412263348311 34-35 22.250085970342994 26.93849315113694 28.85856203644339 21.952858842076676 36-37 22.170373226695496 26.867811887272836 29.022273215276574 21.9395416707551 38-39 21.92344591500774 26.9382669437696 29.1326599701399 22.005627171082757 40-41 22.19942517589572 26.773875742055576 29.0086923641004 22.018006717948303 42-43 22.278192373854854 26.834683272951366 28.781471017228654 22.105653335965123 44-45 22.229764389824616 27.04487736358973 28.570817893566307 22.154540353019346 46-47 22.254796034413708 27.059509041229195 28.449231521338724 22.236463403018373 48-49 22.182598746610328 27.12087601862297 28.444167813292907 22.252357421473796 50-51 22.19899483243573 27.303485093476336 28.24044321933631 22.257076854751624 52-53 22.381230942957114 27.360763808000254 28.05262698861713 22.2053782604255 54-55 22.349285113444274 27.368352197677982 28.006221618848816 22.276141070028928 56-57 22.282653601056694 27.54634225272752 27.921372233321897 22.249631912893893 58-59 22.138669990840828 27.53167875337731 28.20767864148767 22.12197261429419 60-61 22.274605764236192 27.540507349069053 28.135900934999604 22.048985951695155 62-63 22.172640161297196 27.588804053432707 28.033794482365437 22.20476130290466 64-65 22.240110537451347 27.5244077956331 28.016587503340613 22.218894163574944 66-67 22.146408543757175 27.66980576801445 27.829383646939824 22.35440204128855 68-69 22.110717192661124 27.555011586596212 28.028818304899765 22.305452915842892 70-71 22.146132673243464 27.323698224631382 28.09415690790587 22.43601219421928 72-73 22.17407957858012 27.43564509835426 27.89341703582987 22.496858287235753 74-75 22.141929386489284 27.570697719924475 27.82493206597756 22.462440827608685 76-77 22.18129424904756 27.567725052768203 27.751872241988085 22.49910845619615 78-79 22.092468477959315 27.60248877015197 27.740916353303913 22.564126398584797 80-81 22.096382019657547 27.598359810273266 27.762103342626098 22.543154827443097 82-83 22.203350560758054 27.618749080944987 27.697854163453712 22.48004619484325 84-85 22.164207537707473 27.530242294110828 27.65340913655252 22.652141031629174 86-87 22.11514943990608 27.610064634331955 27.661434131351275 22.613351794410693 88-89 22.147402715794115 27.528253241539737 27.618554019876502 22.70579002278965 90-91 22.112137177428888 27.503336596293078 27.75170215183206 22.632824074445974 92-93 22.195925049087844 27.428815453805782 27.72832015717291 22.646939339933464 94-95 22.10277003478323 27.461808452404586 27.65362687730162 22.781794635510565 96-97 22.148831129783556 27.38408842353141 27.645479041125913 22.821601405559118 98-99 22.064730838058146 27.35315065250836 27.62210100879694 22.96001750063655 100-101 21.992954072366288 27.502026575647324 27.606385717988747 22.89863363399764 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1961.0 1 1206.5 2 493.5 3 535.0 4 427.0 5 415.5 6 712.5 7 1371.0 8 2273.5 9 2743.5 10 2384.0 11 1938.5 12 2042.0 13 2372.0 14 2647.0 15 2851.5 16 3039.0 17 3250.0 18 3592.0 19 4005.5 20 4505.0 21 5179.5 22 6082.0 23 7549.5 24 9355.5 25 11762.0 26 15761.0 27 21442.5 28 27983.5 29 35667.0 30 44055.5 31 52805.0 32 61759.0 33 69651.5 34 76591.5 35 82901.5 36 89902.5 37 96948.0 38 101613.0 39 105469.5 40 110461.0 41 115266.5 42 119066.5 43 124006.0 44 129014.5 45 133224.5 46 136606.0 47 138504.5 48 138978.5 49 137468.0 50 132564.5 51 126117.0 52 120708.5 53 115249.5 54 109088.0 55 100541.0 56 90710.5 57 80822.0 58 73255.5 59 65414.0 60 53711.0 61 42999.0 62 34275.0 63 25426.0 64 17995.0 65 13308.0 66 9791.5 67 7039.5 68 5177.5 69 3672.5 70 2672.0 71 1946.5 72 1404.0 73 1018.0 74 685.5 75 446.0 76 261.5 77 153.0 78 98.5 79 64.5 80 35.5 81 18.0 82 14.5 83 11.0 84 9.0 85 7.5 86 7.0 87 3.5 88 1.5 89 2.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018791664419317487 2 0.0 3 0.0016926842759385217 4 0.021058139975235167 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.7379127997915985E-5 14-15 7.172390999739499E-5 16-17 0.0011045482139598827 18-19 1.7213738399374798E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.1558520398853793E-4 28-29 0.00529322455780775 30-31 1.4344781999478998E-4 32-33 1.8648216599322697E-4 34-35 0.01074424171760977 36-37 0.004188676343847867 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.0124042198905897E-4 60-61 0.0022521307739182027 62-63 0.00988355479764103 64-65 0.0020799933899244547 66-67 0.004088262869851514 68-69 0.0027255085799010093 70-71 5.7379127997915985E-5 72-73 0.006541220591762422 74-75 0.006340393643769716 76-77 0.0017500634039364377 78-79 3.1558520398853793E-4 80-81 0.0074449418577296 82-83 0.010055692181634778 84-85 0.0020656486079249758 86-87 0.0016209603659411266 88-89 4.1599867798489094E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.4344781999478996E-5 100-101 8.176525739703028E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3485588.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.2320364575506 #Duplication Level Percentage of deduplicated Percentage of total 1 58.083230569269574 26.853060332507876 2 20.573272210483818 19.022885417724005 3 9.057963892468106 12.563043507232871 4 4.491231590599106 8.30555130543523 5 2.533267261059691 5.855905218501549 6 1.5247987187534557 4.229672997350171 7 0.946250263192729 3.062295366612516 8 0.6242994828607418 2.3090109161638237 9 0.44529092573375295 1.8528035681465387 >10 1.6556633141978694 12.21774310723303 >50 0.05055862517987248 1.5673336991416682 >100 0.013054824928861408 0.9684689488016809 >500 5.591606385971084E-4 0.16966961600984828 >1k 4.349027152152935E-4 0.44122142423531246 >5k 6.212895931647048E-5 0.27462522212836554 >10k+ 6.212895931647048E-5 0.30670935277551375 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10678 0.30634716438087345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9561 0.2743009213940374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4626 0.13271792305917968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 8.606869199687399E-5 0.0 4 0.0 0.0 0.0 8.606869199687399E-5 0.0 5 0.0 0.0 0.0 8.606869199687399E-5 0.0 6 0.0 0.0 0.0 8.606869199687399E-5 0.0 7 0.0 0.0 0.0 8.606869199687399E-5 0.0 8 0.0 0.0 0.0 8.606869199687399E-5 0.0 9 0.0 0.0 0.0 8.606869199687399E-5 0.0 10-11 0.0 0.0 0.0 8.606869199687399E-5 0.0 12-13 0.0 0.0 0.0 1.0041347399635298E-4 0.0 14-15 1.4344781999478998E-5 0.0 0.0 1.1475825599583198E-4 1.4344781999478998E-5 16-17 4.3034345998436995E-5 0.0 0.0 1.1475825599583198E-4 2.8689563998957996E-5 18-19 5.737912799791599E-5 0.0 0.0 1.5779260199426896E-4 2.8689563998957996E-5 20-21 5.737912799791599E-5 0.0 0.0 2.0082694799270596E-4 2.8689563998957996E-5 22-23 5.737912799791599E-5 0.0 0.0 2.8689563998957995E-4 2.8689563998957996E-5 24-25 8.606869199687399E-5 0.0 0.0 5.164121519812439E-4 2.8689563998957996E-5 26-27 1.1475825599583198E-4 0.0 0.0 8.893764839676978E-4 2.8689563998957996E-5 28-29 1.1475825599583198E-4 0.0 0.0 0.0017357186219369588 2.8689563998957996E-5 30-31 1.7213738399374798E-4 0.0 0.0 0.0038300567938608925 2.8689563998957996E-5 32-33 2.8689563998957995E-4 0.0 0.0 0.008678593109684794 2.8689563998957996E-5 34-35 2.8689563998957995E-4 0.0 0.0 0.014990297189455552 2.8689563998957996E-5 36-37 2.8689563998957995E-4 0.0 0.0 0.021345035615224748 2.8689563998957996E-5 38-39 2.8689563998957995E-4 0.0 0.0 0.027111637979015307 2.8689563998957996E-5 40-41 3.1558520398853793E-4 0.0 0.0 0.03564678326870531 2.8689563998957996E-5 42-43 3.4427476798749596E-4 0.0 0.0 0.04620454282032185 2.8689563998957996E-5 44-45 3.7296433198645394E-4 0.0 0.0 0.06271538690172218 2.8689563998957996E-5 46-47 3.8730911398593296E-4 0.0 0.0 0.08509324682090941 2.8689563998957996E-5 48-49 4.016538959854119E-4 0.0 0.0 0.1023787091302816 2.8689563998957996E-5 50-51 4.016538959854119E-4 0.0 0.0 0.12200237090556887 2.8689563998957996E-5 52-53 4.159986779848909E-4 0.0 0.0 0.14542739991071807 2.8689563998957996E-5 54-55 4.303434599843699E-4 0.0 0.0 0.17192221226375579 2.8689563998957996E-5 56-57 4.303434599843699E-4 0.0 0.0 0.20355245657260698 2.8689563998957996E-5 58-59 4.303434599843699E-4 0.0 0.0 0.24112144062924246 2.8689563998957996E-5 60-61 4.5903302398332793E-4 0.0 0.0 0.2767108447699499 5.737912799791599E-5 62-63 4.5903302398332793E-4 0.0 0.0 0.31769388694246137 5.737912799791599E-5 64-65 4.5903302398332793E-4 0.0 0.0 0.36537594230872955 5.737912799791599E-5 66-67 4.5903302398332793E-4 0.0 0.0 0.4270298153424903 5.737912799791599E-5 68-69 4.5903302398332793E-4 0.0 0.0 0.5006472365638165 5.737912799791599E-5 70-71 4.5903302398332793E-4 0.0 0.0 0.5742359682211438 5.737912799791599E-5 72-73 4.5903302398332793E-4 0.0 0.0 0.6554848134661928 5.737912799791599E-5 74-75 4.5903302398332793E-4 0.0 0.0 0.746588523944884 5.737912799791599E-5 76-77 4.5903302398332793E-4 0.0 0.0 0.8447929015133171 5.737912799791599E-5 78-79 4.877225879822859E-4 0.0 0.0 0.9499401535694982 5.737912799791599E-5 80-81 4.877225879822859E-4 0.0 0.0 1.056937882503612 5.737912799791599E-5 82-83 5.307569339807229E-4 0.0 0.0 1.1738335110173663 5.737912799791599E-5 84-85 5.737912799791599E-4 0.0 0.0 1.3092052187464498 5.737912799791599E-5 86-87 5.88136061978639E-4 0.0 0.0 1.4774121324723404 5.737912799791599E-5 88-89 6.024808439781179E-4 0.0 0.0 1.6744807475811827 5.737912799791599E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5730 0.0 59.039925 1 GTATCAA 10075 0.0 39.190098 1 TCAACGC 11840 0.0 32.940548 4 ATCAACG 12075 0.0 32.221252 3 CAACGCA 12170 0.0 32.04182 5 TATCAAC 12320 0.0 31.773285 2 AACGCAG 12695 0.0 30.64191 6 GTGGTAT 2700 0.0 30.268095 1 ACGCAGA 14170 0.0 27.25118 7 TGGTATC 2925 0.0 27.122906 2 CGCAGAG 14500 0.0 26.4672 8 GCAGAGT 16715 0.0 22.36315 9 GAGTACT 10120 0.0 22.246859 12-13 CAGAGTA 15495 0.0 21.426758 10-11 GTACATG 9125 0.0 20.723858 1 TACATGG 8800 0.0 20.459837 2 GTACTTT 11005 0.0 20.17727 14-15 ACATGGG 9235 0.0 19.084583 3 AGAGTAC 14560 0.0 18.431383 10-11 TATATCG 130 0.0075690434 18.268011 5 >>END_MODULE