FastQCFastQC Report
Fri 10 Feb 2017
SRR2031725_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031725_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267797
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14390.537347319051371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.5134486196634018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7900.2949995705702454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.24608192026049583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.1710250674951549No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.1497402883527448No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3090.11538590798253902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.047.7403261
TTAGTAC608.282233E-539.57643
GTATCAA15650.035.5049631
ATGGGTA1201.5007936E-731.6611215
CATGGGT2450.031.0149774
TATCAAC19350.028.4704632
ATCAACG19550.028.1792073
TCAACGC19700.027.9646474
GTGGTAT5450.027.8850231
CAACGCA20000.027.782635
CGAGCCC350.008387146527.14317744-45
GACGACG350.008387146527.14317714-15
AACGCAG20550.027.0390596
TGGTATC5550.026.5268862
GAGTACT11400.025.00029412-13
TTCGTAG1151.2796838E-424.7782696
ACGCAGA22750.024.4242927
CGCAGAG22900.023.8539928
GCAGAGT24650.022.1605039
GTACTTT12700.022.0671914-15
Produced by FastQC (version 0.11.5)