##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031725_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267797 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.543176361198967 33.0 31.0 34.0 30.0 34.0 2 31.830588094713534 34.0 31.0 34.0 30.0 34.0 3 31.91247101349156 34.0 31.0 34.0 30.0 34.0 4 35.268106065415225 37.0 35.0 37.0 35.0 37.0 5 35.27414048701068 37.0 35.0 37.0 35.0 37.0 6 35.31390941646097 37.0 37.0 37.0 35.0 37.0 7 35.30437234173647 37.0 37.0 37.0 35.0 37.0 8 35.299252792226945 37.0 37.0 37.0 35.0 37.0 9 37.0211242097559 39.0 38.0 39.0 35.0 39.0 10-11 36.96475128548863 39.0 38.0 39.0 35.0 39.0 12-13 36.90434918987143 39.0 38.0 39.0 35.0 39.0 14-15 38.237215129370384 41.0 39.0 41.0 34.0 41.0 16-17 38.15493825546963 41.0 38.5 41.0 34.0 41.0 18-19 38.20616549102492 41.0 39.0 41.0 34.5 41.0 20-21 38.23526402461566 41.0 39.0 41.0 34.5 41.0 22-23 38.136728566787525 41.0 39.0 41.0 34.0 41.0 24-25 38.0540484023346 41.0 39.0 41.0 34.0 41.0 26-27 37.939780878799986 41.0 38.0 41.0 34.0 41.0 28-29 37.82568512716722 40.0 38.0 41.0 34.0 41.0 30-31 37.636844326112694 40.0 38.0 41.0 33.5 41.0 32-33 37.379761535790166 40.0 38.0 41.0 33.0 41.0 34-35 37.19463996982789 40.0 38.0 41.0 33.0 41.0 36-37 37.038648677916484 40.0 37.0 41.0 32.0 41.0 38-39 36.83587754903901 40.0 37.0 41.0 31.0 41.0 40-41 36.72099575424669 40.0 37.0 41.0 31.0 41.0 42-43 36.64097805427245 40.0 37.0 41.0 31.0 41.0 44-45 36.41136383155898 40.0 37.0 41.0 30.0 41.0 46-47 36.165274442954924 40.0 37.0 41.0 29.5 41.0 48-49 36.04689559629122 40.0 37.0 41.0 27.0 41.0 50-51 35.49121162671725 39.5 36.5 40.5 25.5 41.0 52-53 35.51846734653488 39.5 37.0 40.5 26.0 41.0 54-55 35.732933901425326 40.0 37.0 41.0 26.0 41.0 56-57 35.61373540405606 40.0 36.5 41.0 24.5 41.0 58-59 35.45563057091752 40.0 36.0 41.0 23.5 41.0 60-61 35.252478556518554 40.0 36.0 41.0 23.0 41.0 62-63 34.984912078925454 39.0 35.0 41.0 22.0 41.0 64-65 34.730439474676714 39.0 35.0 41.0 21.0 41.0 66-67 34.44614390751204 39.0 35.0 41.0 19.5 41.0 68-69 34.10614383282859 38.0 35.0 40.5 18.5 41.0 70-71 33.746412394462965 37.0 35.0 40.0 15.0 41.0 72-73 33.36757133201642 37.0 35.0 39.0 12.5 41.0 74-75 32.95261896137746 36.0 34.0 39.0 8.0 41.0 76-77 32.56857806472813 36.0 34.0 38.5 7.0 40.0 78-79 32.16854744451955 35.5 34.0 37.0 2.0 39.0 80-81 31.794458115662238 35.0 34.0 37.0 2.0 39.0 82-83 31.454857597359194 35.0 34.0 36.5 2.0 39.0 84-85 31.12096289353503 35.0 33.5 36.0 2.0 37.0 86-87 30.850265312904924 35.0 33.0 36.0 2.0 37.0 88-89 30.574491125740764 35.0 33.0 35.0 2.0 36.5 90-91 30.346103578456816 35.0 33.0 35.0 2.0 36.0 92-93 30.133315533781186 35.0 33.0 35.0 2.0 36.0 94-95 29.901016441558347 35.0 32.0 35.0 2.0 36.0 96-97 29.672849210409378 35.0 32.0 35.0 2.0 35.5 98-99 29.462462238187882 35.0 32.0 35.0 2.0 35.0 100-101 28.958694458862496 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03458549716071957 1101 2 0.05323265362282115 1101 3 0.08466274190254808 1101 4 0.02376660091798044 1101 5 -0.23698500964157176 1101 6 0.10677744250713062 1101 7 0.18697101875272182 1101 8 0.032684429626932854 1101 9 0.029992842468388403 1101 10-11 0.026910456269362726 1101 12-13 0.16782983923669548 1101 14-15 -0.04588026367201081 1101 16-17 0.3055876532408135 1101 18-19 0.26073585759545637 1101 20-21 0.34664255661704857 1101 22-23 0.3535531170154016 1101 24-25 0.36201971969558855 1101 26-27 0.30998644900795824 1101 28-29 0.2188336661885657 1101 30-31 0.1572840935410298 1101 32-33 0.06279895737991126 1101 34-35 -0.07811450522661545 1101 36-37 -0.37158084755824206 1101 38-39 -6.194060406272683E-4 1101 40-41 -0.2138943917946321 1101 42-43 -0.3982747646787388 1101 44-45 -0.7018210754771275 1101 46-47 -0.4352447370073165 1101 48-49 -0.6017737598455284 1101 50-51 -0.25573562584917653 1101 52-53 -0.2797998070887857 1101 54-55 -0.3271040308098989 1101 56-57 -0.6341412029633346 1101 58-59 -0.6300914755489231 1101 60-61 -0.6088265659053889 1101 62-63 -0.4537274726806473 1101 64-65 -0.3373265768132825 1101 66-67 -0.5570948078915023 1101 68-69 -0.7844413221439837 1101 70-71 -0.6700353708413971 1101 72-73 -0.3021234292802504 1101 74-75 -0.20075613191892216 1101 76-77 -0.25855796293772926 1101 78-79 -0.38462214876149403 1101 80-81 -0.5608335435629073 1101 82-83 -0.3940661589464334 1101 84-85 -0.4674005215578454 1101 86-87 -0.3584030245811789 1101 88-89 -0.3327697257722022 1101 90-91 -0.6910320638064249 1101 92-93 -0.5534563639305929 1101 94-95 -0.46718544022172637 1101 96-97 -0.32110071531553075 1101 98-99 -0.38702432857443725 1101 100-101 -0.7074459664235491 1102 1 0.33043795339053617 1102 2 0.4573207039372882 1102 3 0.4570492793834262 1102 4 0.36904112072080153 1102 5 0.12749739729366638 1102 6 0.09840586338083313 1102 7 0.17498733695772017 1102 8 0.03778386868864203 1102 9 0.16347065990847653 1102 10-11 0.39115913891426857 1102 12-13 0.16670371311057153 1102 14-15 0.5827318389966933 1102 16-17 0.5505732048301439 1102 18-19 0.6730255057341594 1102 20-21 0.47402229484584524 1102 22-23 0.5688344360703113 1102 24-25 0.5963601922436084 1102 26-27 0.5652771170344764 1102 28-29 0.6078783712710063 1102 30-31 0.6275161180183417 1102 32-33 0.5491792055526119 1102 34-35 0.7277456001617892 1102 36-37 0.7627043810666123 1102 38-39 0.5611263019314947 1102 40-41 0.5025980440374269 1102 42-43 0.9016359951376813 1102 44-45 0.8550376700608666 1102 46-47 1.0015009709648055 1102 48-49 1.0947246253321055 1102 50-51 1.1435717003449426 1102 52-53 1.2708333732613752 1102 54-55 1.3905995217993095 1102 56-57 1.322874775279061 1102 58-59 1.3888401919676454 1102 60-61 1.4308428204621038 1102 62-63 1.4215699945979594 1102 64-65 1.2755835030779323 1102 66-67 1.189229346451512 1102 68-69 1.1845158425678974 1102 70-71 1.4141053736766978 1102 72-73 1.1796885713996659 1102 74-75 1.051729844623658 1102 76-77 1.234727775631022 1102 78-79 1.037441424245479 1102 80-81 1.0358220743191318 1102 82-83 1.2750014936117857 1102 84-85 1.2620954838815557 1102 86-87 1.1803314646250058 1102 88-89 1.0832595110117538 1102 90-91 1.2493044991716467 1102 92-93 1.5305568946109673 1102 94-95 1.3880117380886574 1102 96-97 0.85731590885581 1102 98-99 1.198497514022879 1102 100-101 1.2182297092521281 1103 1 -0.3063507340310636 1103 2 -0.26419828340448603 1103 3 -0.3365417800304158 1103 4 -0.3027002408939765 1103 5 5.159855021261706E-4 1103 6 -0.2778057913118346 1103 7 -0.379066717096336 1103 8 -0.32457215822434904 1103 9 -0.14477429390610297 1103 10-11 -0.24141013922462662 1103 12-13 6.357464096922172E-4 1103 14-15 0.1234434366703212 1103 16-17 0.10080698291201884 1103 18-19 -0.048037329252728966 1103 20-21 -0.0744724023830301 1103 22-23 -0.24884716958597863 1103 24-25 -0.20102263362023365 1103 26-27 6.460302514881278E-4 1103 28-29 -0.6454287333019195 1103 30-31 -0.17402909808383527 1103 32-33 -0.051373879177688764 1103 34-35 0.026325825957208338 1103 36-37 -0.2100690252511015 1103 38-39 -0.3412570941227955 1103 40-41 0.043081565533611865 1103 42-43 -0.1157833229145524 1103 44-45 -0.06761599626368309 1103 46-47 0.15291504326494731 1103 48-49 0.0377055809712914 1103 50-51 0.13399250089232595 1103 52-53 0.21851840006032575 1103 54-55 0.4253241198150519 1103 56-57 0.5289130920768699 1103 58-59 0.6041155939519172 1103 60-61 0.5768971025923406 1103 62-63 0.6044585793852733 1103 64-65 0.8690662263074884 1103 66-67 0.6779680851902512 1103 68-69 0.4867452872024032 1103 70-71 0.32236173130014834 1103 72-73 0.4422042516368663 1103 74-75 0.4474705220099082 1103 76-77 0.15187910417475337 1103 78-79 0.3379317880269781 1103 80-81 0.5800688527431213 1103 82-83 0.6948897362848392 1103 84-85 0.5341618540491915 1103 86-87 0.3757642391717084 1103 88-89 0.5549780650087897 1103 90-91 0.7146084106780926 1103 92-93 0.2477139250591378 1103 94-95 0.528107530083183 1103 96-97 0.5724487632291755 1103 98-99 0.6929951762420608 1103 100-101 0.9581658479224444 1104 1 0.22378662120728876 1104 2 0.3069660940082066 1104 3 0.3580268527014283 1104 4 0.39949923841296453 1104 5 0.318354211554734 1104 6 0.4379496624991006 1104 7 0.43795455949940987 1104 8 0.39908346615845147 1104 9 0.34851953857437223 1104 10-11 0.3313163173693212 1104 12-13 0.29176600944946074 1104 14-15 0.41249990493496824 1104 16-17 0.427245621928094 1104 18-19 0.6008000888278886 1104 20-21 0.3766004048707643 1104 22-23 0.49981967663001825 1104 24-25 0.34097395494247706 1104 26-27 0.56254566217536 1104 28-29 0.5120090977421583 1104 30-31 0.5645201433202374 1104 32-33 0.6795894029840852 1104 34-35 0.8672989833321978 1104 36-37 0.370189525785797 1104 38-39 0.06800765136603104 1104 40-41 0.3613866198472806 1104 42-43 0.44697814579897965 1104 44-45 -0.06402500964011182 1104 46-47 0.05199934167593767 1104 48-49 0.19666060386383322 1104 50-51 0.3801636114049387 1104 52-53 -0.1110623522513734 1104 54-55 -0.22174473735346112 1104 56-57 -0.2671098902375135 1104 58-59 -0.07363824391504181 1104 60-61 -0.09199597578094654 1104 62-63 -0.2370173161170115 1104 64-65 -0.1913611011007177 1104 66-67 0.13257801426826887 1104 68-69 -0.031039074050852378 1104 70-71 -0.23189807658206263 1104 72-73 -0.393131056739108 1104 74-75 -0.044503622886409744 1104 76-77 0.14132160350144574 1104 78-79 -0.1275767854535843 1104 80-81 -0.218270315943478 1104 82-83 -0.08438129343057099 1104 84-85 -0.09599345612227594 1104 86-87 -0.2708377897357366 1104 88-89 -0.4798993925726833 1104 90-91 -0.35066674867193726 1104 92-93 -0.4319790455845478 1104 94-95 -0.36159531577371595 1104 96-97 -0.5464218714777118 1104 98-99 -0.2139713378076742 1104 100-101 -0.34042468982780605 1105 1 0.3319706063754708 1105 2 0.5181782419732492 1105 3 0.6367061146419246 1105 4 0.7469522268476751 1105 5 0.749960108967997 1105 6 0.8574429087249342 1105 7 0.9196884696256546 1105 8 0.919750897958572 1105 9 0.7853165722108173 1105 10-11 0.7744345304884135 1105 12-13 0.7046555653777702 1105 14-15 0.9269432703450562 1105 16-17 0.7795366080674029 1105 18-19 0.7716573325817535 1105 20-21 1.0458420399021051 1105 22-23 0.8720100182915402 1105 24-25 0.9432163845354893 1105 26-27 0.9483936360431144 1105 28-29 1.0641323430851486 1105 30-31 1.1159371116260601 1105 32-33 1.0936354875607606 1105 34-35 1.2202785895827475 1105 36-37 1.4176425142205105 1105 38-39 0.9332058503595846 1105 40-41 1.223150546747604 1105 42-43 1.3427316551130062 1105 44-45 1.5083683866695878 1105 46-47 1.231464336820288 1105 48-49 1.4559260757451682 1105 50-51 1.4298413725067363 1105 52-53 1.767908248966954 1105 54-55 1.7059148371146264 1105 56-57 1.5709496136028633 1105 58-59 1.7101085557671354 1105 60-61 1.7340744753535091 1105 62-63 1.7828555540162938 1105 64-65 1.7628456615019061 1105 66-67 1.6482308791045028 1105 68-69 1.6334616763684977 1105 70-71 1.6646941367135994 1105 72-73 1.2165096248182365 1105 74-75 1.160034207077402 1105 76-77 1.3840866781019585 1105 78-79 1.3785501678438479 1105 80-81 1.6701908829617942 1105 82-83 1.345335312908265 1105 84-85 1.4539668157943844 1105 86-87 1.6201748349663063 1105 88-89 1.6001747665162185 1105 90-91 1.64688776708687 1105 92-93 1.5504346561899744 1105 94-95 1.8026317522937347 1105 96-97 1.8250180508318152 1105 98-99 1.8476601621481805 1105 100-101 1.7209772700909731 1106 1 0.21242738083450874 1106 2 0.23968984994279552 1106 3 0.48139594009372644 1106 4 0.33762127111913287 1106 5 0.31981368051904013 1106 6 0.3936556586306281 1106 7 0.3564704729863095 1106 8 0.2905200759702993 1106 9 0.28576237310927866 1106 10-11 0.21942955809377906 1106 12-13 0.25357364088959145 1106 14-15 0.15311006846582842 1106 16-17 0.40542392331721544 1106 18-19 0.0172850545391654 1106 20-21 0.038109108932651736 1106 22-23 0.22701327606733201 1106 24-25 0.5214030036500574 1106 26-27 0.2646293618412656 1106 28-29 0.21926248265511816 1106 30-31 0.35015404520172666 1106 32-33 0.3662255907808145 1106 34-35 0.4020624790241243 1106 36-37 0.35748511221098056 1106 38-39 0.5267468811884299 1106 40-41 0.33232032830516545 1106 42-43 0.042801954485454985 1106 44-45 0.268707538276189 1106 46-47 0.4547248560068695 1106 48-49 0.8150358456433224 1106 50-51 0.6427713131809227 1106 52-53 0.4979758049492631 1106 54-55 0.4311214486848769 1106 56-57 0.45004326608391665 1106 58-59 0.8765551796826401 1106 60-61 0.6883627370729357 1106 62-63 0.5481242347885598 1106 64-65 0.3473763703253425 1106 66-67 0.618739435052511 1106 68-69 0.584672197269704 1106 70-71 0.5987156037414749 1106 72-73 0.5507145560620117 1106 74-75 0.6965813076569418 1106 76-77 0.8820548547762854 1106 78-79 0.8451065273651253 1106 80-81 0.6617806775504569 1106 82-83 0.9203927526394047 1106 84-85 0.8873281545687668 1106 86-87 0.6965439581102295 1106 88-89 0.5022528845505825 1106 90-91 0.898902444224138 1106 92-93 0.8389107029647747 1106 94-95 0.851249769508506 1106 96-97 0.9860293330237795 1106 98-99 1.2491638828710627 1106 100-101 0.9516449426673574 1107 1 0.17443985002401163 1107 2 0.211706668849569 1107 3 0.18760878625872124 1107 4 0.34675567738272406 1107 5 0.24662968747858827 1107 6 0.43671785958756715 1107 7 0.41040696711419145 1107 8 0.31255864346341156 1107 9 0.7309196878838193 1107 10-11 0.5882312109863435 1107 12-13 0.531864926955997 1107 14-15 0.6264136787311685 1107 16-17 0.6039160213308463 1107 18-19 0.432864291888734 1107 20-21 0.4705253768752371 1107 22-23 0.5744650667009381 1107 24-25 0.4520975085072436 1107 26-27 0.5827024370913776 1107 28-29 0.5880822593696351 1107 30-31 0.7876631071126994 1107 32-33 0.6942123966384344 1107 34-35 0.7404886063258473 1107 36-37 0.7564810524748609 1107 38-39 0.2916265390106787 1107 40-41 0.4931148766595186 1107 42-43 0.3891300681580674 1107 44-45 0.32403956669433853 1107 46-47 0.49735208523696883 1107 48-49 0.3846131981154173 1107 50-51 0.5115185946076295 1107 52-53 0.3521515335269001 1107 54-55 0.3331078195707633 1107 56-57 0.4585433385792044 1107 58-59 0.5932617187576028 1107 60-61 0.6213952149618649 1107 62-63 0.7015605114305785 1107 64-65 0.7170842143154843 1107 66-67 0.21524878694464178 1107 68-69 0.1421972081439904 1107 70-71 0.048766350442946305 1107 72-73 -0.06159876857188351 1107 74-75 0.019783424519346227 1107 76-77 0.46475385434131056 1107 78-79 0.3685225053265597 1107 80-81 0.45071064710242936 1107 82-83 0.21318309626707688 1107 84-85 0.5138143079688042 1107 86-87 -0.025690346659960284 1107 88-89 0.19693897995438192 1107 90-91 0.26821156413134517 1107 92-93 0.5611993792008221 1107 94-95 0.34231472528638207 1107 96-97 0.2546606219899985 1107 98-99 0.2659465419982219 1107 100-101 -0.16577929975688122 1108 1 -0.26415664120406035 1108 2 0.1160508626674428 1108 3 0.1508502231843707 1108 4 0.10155935323903265 1108 5 0.13865140844767154 1108 6 0.041563306538272116 1108 7 0.005853917824296673 1108 8 0.09430467520944319 1108 9 0.012248008685823208 1108 10-11 0.07831057606571079 1108 12-13 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402.0 8 410.0 9 392.0 10 455.0 11 546.0 12 731.0 13 964.0 14 1076.0 15 1468.0 16 1908.0 17 2826.0 18 2669.0 19 1320.0 20 1031.0 21 947.0 22 936.0 23 1004.0 24 1052.0 25 1225.0 26 1363.0 27 1569.0 28 1810.0 29 2197.0 30 2780.0 31 3447.0 32 4640.0 33 6087.0 34 8556.0 35 14195.0 36 23243.0 37 49988.0 38 88874.0 39 30417.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.69796358086574 21.849236155791303 15.08947675549504 21.36332350784792 2 16.41048469454555 23.155882583990152 39.418980035387904 21.014652686076392 3 18.11418290361072 28.3579521438751 32.389851239793074 21.13801371272111 4 13.051512533058022 20.965612176573785 39.0688766444846 26.913998645883595 5 12.85028202097578 38.36794386510869 34.8879396759377 13.893834437977828 6 26.154576798522143 38.94261332615345 20.047192742339593 14.855617132984811 7 23.397396341714256 35.70998492855086 24.278496445307095 16.61412228442779 8 23.680244711443176 34.505285752214704 22.289532827008006 19.524936709334114 9 25.80729595118084 17.560836985297417 23.203308455107017 33.42855860841472 10-11 22.56535840803088 28.32474973594923 29.450680923850562 19.65921093216933 12-13 24.453572857575566 27.778317920566604 28.710375852127186 19.05773336973065 14-15 22.392441849592508 29.159731754479797 27.6610426866442 20.786783709283494 16-17 19.744520086780877 31.211565796364184 29.042043839305755 20.001870277549187 18-19 19.433093132295102 29.326470802762717 32.058703064012676 19.18173300092951 20-21 21.613095059992222 28.30349929684929 31.417185302654023 18.666220340504474 22-23 21.437213311866202 28.394957841767788 30.93866346529636 19.229165381069656 24-25 20.490053099992522 28.89724029616334 31.60103956323386 19.011667040610273 26-27 19.960809464065683 28.829351018935235 32.00703784242637 19.202801674572708 28-29 19.720084890754574 28.907358756930098 32.17284804741915 19.199708304896177 30-31 20.369366303831644 28.554281291248955 31.81530936951961 19.261043035399787 32-33 20.251462972311028 28.928524688242003 31.418475517415445 19.40153682203152 34-35 20.484421689585663 29.160854329427693 31.214027398079963 19.14069658290668 36-37 20.24492955797489 28.86458750182323 31.235531054704303 19.65495188549758 38-39 19.830119959315542 29.342729149216222 31.566351561565153 19.260799329903076 40-41 20.000448742399428 29.1089845707405 31.638208919503093 19.25235776735698 42-43 20.232439436177312 28.835054379834308 31.102984206757423 19.829521977230957 44-45 20.46210606822759 29.07610575781319 30.79423078001904 19.66755739394019 46-47 20.3846822706202 29.293035066821993 30.510173361332438 19.812109301225366 48-49 20.24188676916402 29.525558570672494 30.281478101336607 19.951076558826884 50-51 20.276415831734525 29.34230160778198 30.10935460587089 20.271927954612604 52-53 20.46882682984742 29.83078036222089 29.65578358732171 20.04460922060998 54-55 20.77093919305448 29.633617869307876 29.56818581208661 20.02725712555103 56-57 20.570130331532003 29.846855775164084 29.57198152545859 20.01103236784532 58-59 20.315494453512525 29.765931310683698 29.80108181279366 20.117492423010116 60-61 20.362794663156635 30.007964879741532 29.63290504965897 19.99633540744286 62-63 20.386390874399595 29.651844359227965 29.97885376565635 19.98291100071609 64-65 20.359988031566743 29.858435875378685 29.548191644537532 20.233384448517036 66-67 20.405316459483313 29.89517419856692 29.335330371434125 20.36417897051564 68-69 20.31835258521224 29.87846725497566 29.452102185494986 20.351077974317114 70-71 20.32673102561082 29.918120913291187 29.56846598717683 20.186682073921165 72-73 20.2942776845967 29.46684841651381 29.798373600647714 20.440500298241776 74-75 20.33893552186349 29.777906563721 29.327550429044138 20.55560748537137 76-77 19.885182137781435 30.191487770214675 29.5145485825417 20.40878150946219 78-79 19.99038824748206 29.869923442578195 29.520235170038262 20.619453139901488 80-81 19.964208376810618 30.011668742356413 29.306121227761135 20.718001653071834 82-83 20.253363404809193 30.054270785411596 29.293881352318756 20.398484457460455 84-85 20.123003622043417 29.90326881471107 29.10387616613156 20.86985139711396 86-87 19.860378918076872 30.03648549837021 29.504444274058265 20.598691309494647 88-89 20.325387194306753 29.908621582658483 29.17714548558457 20.588845737450193 90-91 20.038334953998056 29.916598100082282 29.433016680379982 20.61205026553968 92-93 20.211675637663976 29.871737804992087 29.3804214062085 20.53616515113543 94-95 20.00474927919404 29.6798163113433 29.38289287196766 20.932541537495 96-97 20.119950568998686 29.57204044966507 29.36022000908603 20.947788972250216 98-99 19.881955272377834 29.67364764564895 29.413029415833776 21.03136766613945 100-101 19.7240791102898 29.934760570545745 29.339226329197267 21.001933989967192 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1157.0 1 673.0 2 186.5 3 161.0 4 121.5 5 121.5 6 175.5 7 283.0 8 410.5 9 488.0 10 420.0 11 314.0 12 292.5 13 309.0 14 333.5 15 347.0 16 359.5 17 362.0 18 405.0 19 456.5 20 499.5 21 552.5 22 631.5 23 763.5 24 921.5 25 1138.5 26 1519.0 27 2212.0 28 2933.5 29 3654.0 30 4716.5 31 5949.0 32 7259.5 33 8913.5 34 10549.5 35 11532.0 36 12568.5 37 13459.5 38 12741.5 39 12004.0 40 11423.5 41 10232.5 42 9647.5 43 9572.0 44 9608.5 45 9542.5 46 8987.5 47 8433.0 48 8302.0 49 7863.5 50 7268.0 51 6695.0 52 5949.0 53 5354.0 54 4792.5 55 4074.5 56 3485.5 57 2959.5 58 2493.5 59 2101.5 60 1663.0 61 1287.0 62 988.5 63 741.0 64 559.0 65 423.5 66 328.5 67 287.0 68 255.0 69 214.5 70 170.0 71 133.5 72 117.5 73 90.5 74 74.0 75 75.5 76 66.0 77 46.0 78 38.5 79 34.0 80 25.5 81 21.0 82 17.0 83 12.0 84 6.0 85 4.5 86 4.0 87 4.5 88 3.5 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17363898774071404 2 0.17699974234214724 3 0.16953139878340684 4 0.17326557056277703 5 0.16467697547022558 6 0.14301877914987846 7 0.15086053988655584 8 0.1418985276160674 9 0.13480360123526403 10-11 0.12304096013024791 12-13 0.14115169326019336 14-15 0.16056938651291836 16-17 0.1706516503172179 18-19 0.1685978558385643 20-21 0.16019596933498137 22-23 0.1536611687210835 24-25 0.14077827608225635 26-27 0.143952322094721 28-29 0.14731307669615418 30-31 0.13667068712494912 32-33 0.13517701841320104 34-35 0.1516073742424299 36-37 0.15459471166592606 38-39 0.13928460737050824 40-41 0.14301877914987846 42-43 0.13909789878153975 44-45 0.15403458589902053 46-47 0.15216750000933543 48-49 0.1644902668812571 50-51 0.15310104295417798 52-53 0.13573714418010657 54-55 0.1284555092103347 56-57 0.15011370553068182 58-59 0.1402181503153508 60-61 0.13928460737050824 62-63 0.13947131595947676 64-65 0.15944913497910732 66-67 0.1497402883527448 68-69 0.1570219233225167 70-71 0.1458194079844061 72-73 0.1480599110520282 74-75 0.12770867485446064 76-77 0.15571496319973713 78-79 0.1553415460218001 80-81 0.1553415460218001 82-83 0.16206305522466644 84-85 0.15179408283139842 86-87 0.16075609510188688 88-89 0.14656624234028012 90-91 0.15571496319973713 92-93 0.16953139878340684 94-95 0.14488586503956355 96-97 0.13200297240073638 98-99 0.13293651534557893 100-101 0.17699974234214724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 267797.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.429038212601455 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6704779368738 40.44296138215261 2 16.66274631248294 18.80525496787968 3 5.722757587155008 9.687891197010746 4 2.254738245780677 5.089308425222871 5 1.0770370448650282 3.0388082280538007 6 0.5752628987007317 1.9476919255845278 7 0.37989758501481435 1.5006078739173179 8 0.26164810719690307 1.1811640831415107 9 0.183259374960448 0.9307035245214521 >10 1.1225847083928087 11.65837881413422 >50 0.07086985857571115 2.640172402633762 >100 0.016046005715255357 1.4687326612536777 >500 0.0013371671429379464 0.5466718793107062 >1k 0.0013371671429379464 1.0616526351831106 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1439 0.537347319051371 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1375 0.5134486196634018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 790 0.2949995705702454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.24608192026049583 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 458 0.1710250674951549 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 401 0.1497402883527448 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 309 0.11538590798253902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.7341717793701947E-4 0.0 12-13 0.0 0.0 0.0 3.7341717793701947E-4 0.0 14-15 0.0 0.0 0.0 3.7341717793701947E-4 0.0 16-17 0.0 0.0 0.0 3.7341717793701947E-4 0.0 18-19 0.0 0.0 0.0 7.468343558740389E-4 0.0 20-21 0.0 0.0 0.0 0.0011202515338110583 0.0 22-23 0.0 0.0 0.0 0.0014936687117480779 0.0 24-25 0.0 0.0 0.0 0.002800628834527646 0.0 26-27 0.0 0.0 0.0 0.005787966258023801 0.0 28-29 0.0 0.0 0.0 0.010082263804299526 0.0 30-31 0.0 0.0 0.0 0.017177190185102897 0.0 32-33 0.0 0.0 0.0 0.029126539879087518 0.0 34-35 0.0 0.0 0.0 0.04779739877593849 0.0 36-37 0.0 0.0 0.0 0.0653480061389784 0.0 38-39 0.0 0.0 0.0 0.07935115031161663 0.0 40-41 0.0 0.0 0.0 0.0941011288401289 0.0 42-43 0.0 0.0 0.0 0.11370553068182243 0.0 44-45 0.0 0.0 0.0 0.15627508896664266 0.0 46-47 0.0 0.0 0.0 0.20649969939917176 0.0 48-49 0.0 0.0 0.0 0.24981609203986602 0.0 50-51 0.0 0.0 0.0 0.2854774325328514 0.0 52-53 0.0 0.0 0.0 0.325619779161081 0.0 54-55 0.0 0.0 0.0 0.3691228803907437 0.0 56-57 0.0 0.0 0.0 0.43764493254218684 0.0 58-59 0.0 0.0 0.0 0.5018726871473542 0.0 60-61 1.8670858896850973E-4 0.0 0.0 0.5668472761083956 0.0 62-63 3.7341717793701947E-4 0.0 0.0 0.6411572945178624 0.0 64-65 3.7341717793701947E-4 0.0 0.0 0.7382457607814875 0.0 66-67 3.7341717793701947E-4 0.0 0.0 0.8523247086412469 0.0 68-69 3.7341717793701947E-4 0.0 0.0 0.9824605951522982 0.0 70-71 3.7341717793701947E-4 0.0 0.0 1.1144635675530346 0.0 72-73 3.7341717793701947E-4 0.0 0.0 1.2628968957829998 0.0 74-75 3.7341717793701947E-4 0.0 0.0 1.4326150031553753 0.0 76-77 3.7341717793701947E-4 0.0 0.0 1.6193235921238849 0.0 78-79 3.7341717793701947E-4 0.0 0.0 1.834785303793545 0.0 80-81 3.7341717793701947E-4 0.0 0.0 2.045019174972087 0.0 82-83 3.7341717793701947E-4 0.0 0.0 2.253199251671975 0.0 84-85 3.7341717793701947E-4 0.0 0.0 2.5039488866566844 0.0 86-87 3.7341717793701947E-4 0.0 0.0 2.799695291582803 0.0 88-89 3.7341717793701947E-4 0.0 0.0 3.093014484852332 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 955 0.0 47.740326 1 TTAGTAC 60 8.282233E-5 39.5764 3 GTATCAA 1565 0.0 35.504963 1 ATGGGTA 120 1.5007936E-7 31.661121 5 CATGGGT 245 0.0 31.014977 4 TATCAAC 1935 0.0 28.470463 2 ATCAACG 1955 0.0 28.179207 3 TCAACGC 1970 0.0 27.964647 4 GTGGTAT 545 0.0 27.885023 1 CAACGCA 2000 0.0 27.78263 5 CGAGCCC 35 0.0083871465 27.143177 44-45 GACGACG 35 0.0083871465 27.143177 14-15 AACGCAG 2055 0.0 27.039059 6 TGGTATC 555 0.0 26.526886 2 GAGTACT 1140 0.0 25.000294 12-13 TTCGTAG 115 1.2796838E-4 24.778269 6 ACGCAGA 2275 0.0 24.424292 7 CGCAGAG 2290 0.0 23.853992 8 GCAGAGT 2465 0.0 22.160503 9 GTACTTT 1270 0.0 22.06719 14-15 >>END_MODULE