FastQCFastQC Report
Fri 10 Feb 2017
SRR2031725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267797
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT32431.2109919080497542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.7262964110875028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.7147204785714553No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT12650.4723727300903296RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.3360754601433175No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT8710.32524636198314394RNA PCR Primer, Index 31 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.23263890185476313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.21098070553441597No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.1747592392745251No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT3890.14525928221750056RNA PCR Primer, Index 31 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.053.4124761
GTTTCGA405.378488E-447.504021
GTATCAA17200.039.2184331
TGGTATC5100.033.5322462
GTGGTAT5200.032.8873941
ATCAACG20850.032.125023
TATCAAC21150.031.893952
CGCTAGA300.00394384131.6752662-63
CGGAGAT753.0706663E-431.6693421
TCAACGC21300.031.4463214
CAACGCA21700.030.8608995
AACGCAG22100.030.3023326
ACGCAGA25000.026.7872627
CGCAGAG25100.026.6805388
GTATAGT1257.3584015E-626.6022471
CATATAG908.925149E-426.3911193
CGAAATC458.9466193E-426.38619212-13
GAGTACT15750.025.48152412-13
GCAGAGT26350.024.693879
TATACAC11450.024.478053
Produced by FastQC (version 0.11.5)