##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267797
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.759134717715284	34.0	31.0	34.0	31.0	34.0
2	32.926504031038434	34.0	31.0	34.0	31.0	34.0
3	32.956896455150726	34.0	31.0	34.0	31.0	34.0
4	36.36050067775218	37.0	37.0	37.0	35.0	37.0
5	36.34528392775125	37.0	37.0	37.0	35.0	37.0
6	36.38536652763101	37.0	37.0	37.0	35.0	37.0
7	36.33771849572624	37.0	37.0	37.0	35.0	37.0
8	36.33818526719866	37.0	37.0	37.0	35.0	37.0
9	38.18148448264917	39.0	39.0	39.0	37.0	39.0
10-11	38.153280283199585	39.0	39.0	39.0	37.0	39.0
12-13	38.109691296019	39.0	39.0	39.0	37.0	39.0
14-15	39.54286642494128	41.0	39.0	41.0	37.0	41.0
16-17	39.49914487466252	41.0	39.0	41.0	36.5	41.0
18-19	39.56940331669138	41.0	39.5	41.0	37.0	41.0
20-21	39.60701949611086	41.0	40.0	41.0	37.0	41.0
22-23	39.51803604969436	41.0	39.0	41.0	36.5	41.0
24-25	39.43256272475047	41.0	39.0	41.0	36.0	41.0
26-27	39.30170427600011	41.0	39.0	41.0	36.0	41.0
28-29	39.19434497025732	41.0	39.0	41.0	35.5	41.0
30-31	38.97361994346464	40.0	38.5	41.0	35.0	41.0
32-33	38.69102342445957	40.0	38.0	41.0	35.0	41.0
34-35	38.503627747883655	40.0	38.0	41.0	35.0	41.0
36-37	38.35696815124889	40.0	38.0	41.0	35.0	41.0
38-39	38.363239692752344	40.0	38.0	41.0	35.0	41.0
40-41	38.2829886070419	40.0	38.0	41.0	35.0	41.0
42-43	38.09033708368652	40.0	38.0	41.0	34.0	41.0
44-45	37.80145221940499	40.0	38.0	41.0	33.5	41.0
46-47	37.41787249297042	40.0	38.0	41.0	33.0	41.0
48-49	37.030112361228845	40.0	38.0	41.0	32.5	41.0
50-51	36.731720295597036	40.0	37.0	41.0	31.0	41.0
52-53	36.79706643465013	40.0	37.0	41.0	31.0	41.0
54-55	36.74274917194741	40.0	37.0	41.0	31.0	41.0
56-57	36.545153605156145	40.0	37.0	41.0	31.0	41.0
58-59	36.37579397827459	40.0	36.5	41.0	31.0	41.0
60-61	36.081910925066374	40.0	36.0	41.0	29.5	41.0
62-63	35.77670586302311	39.5	36.0	41.0	28.5	41.0
64-65	35.372873855943126	39.0	35.0	41.0	27.5	41.0
66-67	34.89625163836787	39.0	35.0	41.0	25.0	41.0
68-69	34.332681097995874	38.5	35.0	40.5	21.0	41.0
70-71	33.943658816192865	37.5	35.0	40.0	18.5	41.0
72-73	33.546525913285066	37.0	35.0	39.0	15.5	41.0
74-75	33.147983360530546	36.5	34.0	39.0	12.5	41.0
76-77	32.22416980025915	35.5	33.5	38.0	8.0	39.0
78-79	32.34850278382506	35.5	34.0	37.0	7.0	39.0
80-81	32.06428563426775	35.0	34.0	37.0	3.5	39.0
82-83	31.739504176671133	35.0	34.0	37.0	2.0	39.0
84-85	31.402567989932674	35.0	34.0	36.0	2.0	37.0
86-87	31.14521260507026	35.0	34.0	36.0	2.0	37.0
88-89	30.894856925208273	35.0	33.5	35.5	2.0	37.0
90-91	30.679089011452703	35.0	33.0	35.0	2.0	36.0
92-93	30.501043701012335	35.0	33.0	35.0	2.0	36.0
94-95	30.31428096655302	35.0	33.0	35.0	2.0	36.0
96-97	30.16246634577684	35.0	33.0	35.0	2.0	36.0
98-99	29.97367035478366	35.0	33.0	35.0	2.0	35.0
100-101	29.37933770729321	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1784020592534219
1101	2	0.17769099618544715
1101	3	0.22459751061118993
1101	4	0.04159908481361896
1101	5	0.14547499476467607
1101	6	0.10668181796830822
1101	7	0.14612690863282296
1101	8	0.044059473467463306
1101	9	-0.11794722352165365
1101	10-11	0.04400119887593945
1101	12-13	0.05508558568369182
1101	14-15	0.07882651361861548
1101	16-17	0.19239188255386352
1101	18-19	0.03273901940926294
1101	20-21	0.10250101745897666
1101	22-23	-0.08785743606553353
1101	24-25	-0.13938124427581045
1101	26-27	-0.009274760653752878
1101	28-29	0.20397766982182475
1101	30-31	0.22267263470720167
1101	32-33	0.46836779823046726
1101	34-35	-0.0021789032740997527
1101	36-37	0.30967407082457754
1101	38-39	0.202203883206586
1101	40-41	0.08542893971667098
1101	42-43	0.24271822950974098
1101	44-45	0.3961095816657334
1101	46-47	0.2985169934439682
1101	48-49	-0.02021673912329902
1101	50-51	0.16793262922923446
1101	52-53	0.04674213980594999
1101	54-55	-0.08301645411255265
1101	56-57	-0.1531527769165848
1101	58-59	-0.32594077701529756
1101	60-61	-0.4948224410260167
1101	62-63	-0.3696810956472305
1101	64-65	-0.18663480825933476
1101	66-67	-0.6910618525153112
1101	68-69	-0.4324255436693676
1101	70-71	-0.34456309356171033
1101	72-73	-0.3346886023449045
1101	74-75	-0.5785968995462198
1101	76-77	-0.6212648913134586
1101	78-79	-0.517613095267194
1101	80-81	-0.59577641028784
1101	82-83	-0.5822661541277299
1101	84-85	-0.28439681881576107
1101	86-87	-0.26171848733145353
1101	88-89	-0.36271840021543866
1101	90-91	-0.5243554802244432
1101	92-93	-0.3646527995535038
1101	94-95	-0.11079082162503084
1101	96-97	-0.05850699189127795
1101	98-99	-0.09418954063553997
1101	100-101	-0.40805314239486634
1102	1	0.255616065712708
1102	2	0.18032570636732714
1102	3	0.25944367753282904
1102	4	0.14881479108592544
1102	5	0.1943871144315139
1102	6	0.2418169531034451
1102	7	0.30765164091227604
1102	8	0.09807103219600322
1102	9	0.2739871636957574
1102	10-11	0.23714245333794537
1102	12-13	0.28307300706734395
1102	14-15	0.2658271935438208
1102	16-17	0.3449925964753291
1102	18-19	0.48990373129095843
1102	20-21	0.48302540977582
1102	22-23	0.34510618793582637
1102	24-25	0.10636922317276998
1102	26-27	0.27433904182797875
1102	28-29	0.4247833811935777
1102	30-31	0.4607739435632183
1102	32-33	0.270467067310868
1102	34-35	0.07815850961048199
1102	36-37	0.050750052466604245
1102	38-39	-0.0462450374121417
1102	40-41	-0.0723774515802873
1102	42-43	0.0975541975532579
1102	44-45	0.139437815560008
1102	46-47	-0.005558308272838985
1102	48-49	0.14054307729688986
1102	50-51	-0.03632115285473958
1102	52-53	-0.0044859751014101334
1102	54-55	0.26527523380178053
1102	56-57	-0.02955512265855731
1102	58-59	-0.117649939007471
1102	60-61	-0.19265942895734156
1102	62-63	0.2670263665319297
1102	64-65	0.17036842138539754
1102	66-67	-0.08437733781193657
1102	68-69	-0.07200144032074007
1102	70-71	0.11793478167201243
1102	72-73	0.09944364310809561
1102	74-75	0.23503985041128317
1102	76-77	0.5670106483771775
1102	78-79	0.28614349150826257
1102	80-81	-0.003030362353115379
1102	82-83	-0.09807441521900984
1102	84-85	-0.042385940012433565
1102	86-87	0.009359194123582881
1102	88-89	0.010093090520580716
1102	90-91	0.011319345544727355
1102	92-93	0.19331082444785608
1102	94-95	0.4082683318680864
1102	96-97	0.28064820103750066
1102	98-99	0.30943106109826957
1102	100-101	0.6267717768640182
1103	1	0.15879772838804485
1103	2	-0.022890167734622935
1103	3	0.04037650909857149
1103	4	-0.04487890260776339
1103	5	-0.02268438745011281
1103	6	0.0033062905421488153
1103	7	0.04547807494124356
1103	8	0.06083759622838869
1103	9	-0.04822745598721667
1103	10-11	0.05747544455701359
1103	12-13	0.07526709989434721
1103	14-15	0.0631530003633003
1103	16-17	0.1341361704290307
1103	18-19	0.00837790166385588
1103	20-21	0.22777965769462583
1103	22-23	0.010432907060021535
1103	24-25	-0.3297124280987518
1103	26-27	-0.0529217632809349
1103	28-29	0.06599104455314375
1103	30-31	-0.08789181079873032
1103	32-33	0.13362136084491993
1103	34-35	0.1353201015822023
1103	36-37	0.3710545341634841
1103	38-39	0.265962739985504
1103	40-41	0.4711565887892064
1103	42-43	0.32788262724877626
1103	44-45	0.25073899015232115
1103	46-47	0.3596486708581921
1103	48-49	0.6378916917341186
1103	50-51	0.5134564728722566
1103	52-53	0.4246678622802804
1103	54-55	0.5543332791889384
1103	56-57	0.5371286497214172
1103	58-59	0.4603521136781197
1103	60-61	0.24020064630779814
1103	62-63	0.2932836364600817
1103	64-65	0.5502292949171661
1103	66-67	0.4458997747436584
1103	68-69	0.008651997927636046
1103	70-71	0.011708302027813033
1103	72-73	-0.01319747902669377
1103	74-75	0.241311945290974
1103	76-77	0.09178542315195415
1103	78-79	0.1842219497133044
1103	80-81	0.16782720407407226
1103	82-83	0.3891533188441656
1103	84-85	0.26756958513297135
1103	86-87	0.493336842658195
1103	88-89	0.3162283396938044
1103	90-91	0.16783662231516772
1103	92-93	0.20568395707415377
1103	94-95	0.30936879942424866
1103	96-97	0.3116665611697833
1103	98-99	0.48179963220102096
1103	100-101	0.23468031831686176
1104	1	0.12446789626666543
1104	2	0.06596879626573582
1104	3	0.2705228418034835
1104	4	0.09838350873975088
1104	5	0.1589836258365338
1104	6	0.1173201158406485
1104	7	0.1681461780025586
1104	8	0.11120118362402565
1104	9	0.22554936419412286
1104	10-11	0.2189710904857307
1104	12-13	0.2876829361453588
1104	14-15	0.33875224625610656
1104	16-17	0.3226809230998242
1104	18-19	0.13924243169348216
1104	20-21	0.43312125029719084
1104	22-23	0.3175520380412564
1104	24-25	0.24819402669970003
1104	26-27	0.17524952678100902
1104	28-29	0.323949568657639
1104	30-31	0.1734153083322454
1104	32-33	0.5558226356451641
1104	34-35	0.33024783297640425
1104	36-37	0.19879490583060289
1104	38-39	0.36748890930052625
1104	40-41	0.548084499732731
1104	42-43	0.6488001243310961
1104	44-45	0.6469325443313281
1104	46-47	0.9767686995860814
1104	48-49	1.0178001215752133
1104	50-51	1.2196688797310387
1104	52-53	1.1860737335434024
1104	54-55	1.2373568899259908
1104	56-57	1.348161956122354
1104	58-59	1.280030720375315
1104	60-61	1.6004304694517018
1104	62-63	1.5662883945173647
1104	64-65	1.7005390175134423
1104	66-67	1.7145684164529698
1104	68-69	1.8051597634362153
1104	70-71	1.7750173962014344
1104	72-73	1.8147493743712673
1104	74-75	1.77456448142528
1104	76-77	1.6010052813079767
1104	78-79	1.6566341949776913
1104	80-81	1.6780794708843771
1104	82-83	1.5114713007096867
1104	84-85	1.5563297970011796
1104	86-87	1.3030145516087295
1104	88-89	1.0200796728475865
1104	90-91	1.198476715680819
1104	92-93	1.2923524192341311
1104	94-95	1.2931734497519258
1104	96-97	0.9111638230424823
1104	98-99	0.9917197327486456
1104	100-101	1.1054855970671973
1105	1	0.10126669559570445
1105	2	0.18970853317754433
1105	3	0.11021561129231827
1105	4	0.10726494248213925
1105	5	0.15414562813193555
1105	6	0.18344530344548815
1105	7	0.26268135942746795
1105	8	0.24062645068502775
1105	9	0.20208330963422583
1105	10-11	0.2093304089283876
1105	12-13	0.3498088300272286
1105	14-15	0.23729554986694978
1105	16-17	0.2602107191623304
1105	18-19	0.18055080990483674
1105	20-21	0.16549522398837269
1105	22-23	0.21854082589328527
1105	24-25	0.23412145673413676
1105	26-27	0.3174495797517878
1105	28-29	0.33262794775183124
1105	30-31	0.39835571620724153
1105	32-33	0.21065621911828458
1105	34-35	-0.041509913999853154
1105	36-37	0.281685344605215
1105	38-39	0.4206627417363009
1105	40-41	0.22362082885783252
1105	42-43	0.09829248771062993
1105	44-45	0.4614818512969876
1105	46-47	0.2743938430486068
1105	48-49	0.6293565795958713
1105	50-51	0.6901376711144636
1105	52-53	0.6078182378252066
1105	54-55	0.33640341592581535
1105	56-57	0.41187697333786133
1105	58-59	0.48836478941389316
1105	60-61	0.373777339761574
1105	62-63	0.6529839008297174
1105	64-65	1.0496252715966037
1105	66-67	0.9385376934018481
1105	68-69	0.866084986354899
1105	70-71	0.8280764586186748
1105	72-73	0.5298107321888779
1105	74-75	0.6785372897593405
1105	76-77	0.8642612184493217
1105	78-79	0.7400984838449958
1105	80-81	0.4860074306265929
1105	82-83	0.6568319434217926
1105	84-85	0.7085766408256724
1105	86-87	0.5927205637920103
1105	88-89	0.6116220053275008
1105	90-91	0.5396173530958421
1105	92-93	0.6818906906210849
1105	94-95	0.23514396268562265
1105	96-97	0.16949347784958135
1105	98-99	0.07241188424506717
1105	100-101	0.42647085353404535
1106	1	-0.0553808816478778
1106	2	-0.09604984273549633
1106	3	0.0973559699905735
1106	4	0.006085284720057871
1106	5	-0.03809743623485673
1106	6	-0.07100413244491222
1106	7	-0.12009015046587734
1106	8	-0.12547896408126746
1106	9	-0.05584680250184704
1106	10-11	-0.15173402644762035
1106	12-13	-0.05508126290511228
1106	14-15	0.006687888949969079
1106	16-17	0.05244178119724552
1106	18-19	0.09873777194317768
1106	20-21	-0.07547730781780615
1106	22-23	-0.17110630554938666
1106	24-25	-0.13248119749582798
1106	26-27	-0.3010648744850286
1106	28-29	-0.24620165342781775
1106	30-31	-0.2625526888543206
1106	32-33	-0.21883008107717927
1106	34-35	-0.22116767607933951
1106	36-37	-0.17356310366474048
1106	38-39	-0.41336215452925984
1106	40-41	-0.04927790575346336
1106	42-43	-0.3386009658746332
1106	44-45	-0.49621850191449113
1106	46-47	-0.14369024185931778
1106	48-49	-0.07986121128921297
1106	50-51	-0.15038563055558285
1106	52-53	0.18055236902784344
1106	54-55	0.23087719940374285
1106	56-57	0.26757465174394923
1106	58-59	0.02621500031201407
1106	60-61	-0.3409358045064508
1106	62-63	-0.028577088162428765
1106	64-65	0.27313441051502707
1106	66-67	0.6046071011725118
1106	68-69	0.6379978151330619
1106	70-71	0.03569965398234132
1106	72-73	0.28569187481087255
1106	74-75	0.617196800750051
1106	76-77	0.36175787494258316
1106	78-79	0.18198147825534505
1106	80-81	0.4197974241110316
1106	82-83	0.4258813587367598
1106	84-85	0.46014551706447904
1106	86-87	0.579346536838198
1106	88-89	0.5062586775952589
1106	90-91	0.40853474276012136
1106	92-93	0.35478428137585993
1106	94-95	0.7226529644345341
1106	96-97	0.40379614236725914
1106	98-99	0.38436963603684404
1106	100-101	0.4543162634994111
1107	1	-0.07499085699947727
1107	2	-0.042765806197870404
1107	3	0.050813995218938146
1107	4	0.14810355353258586
1107	5	-2.5489599996575407E-4
1107	6	0.04089234428409583
1107	7	0.03548473716642064
1107	8	0.0641687088199916
1107	9	0.17334704989774252
1107	10-11	0.020333539160610314
1107	12-13	0.1843887965410289
1107	14-15	-0.03467067274351621
1107	16-17	-1.3542628427387626E-4
1107	18-19	-0.02171314874697572
1107	20-21	-0.042401332756185184
1107	22-23	0.04893501676465917
1107	24-25	-0.012229638847138347
1107	26-27	-0.2709430824015229
1107	28-29	-0.4765916780762183
1107	30-31	-0.36384672974165966
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2314	98-99	-0.8447914106316183
2314	100-101	-0.45363429686550205
2315	1	-0.7581319288572033
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2315	3	-0.2867490905777501
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2315	10-11	-0.17374240412225816
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2315	28-29	-0.3208478664924641
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2315	100-101	0.5458819829605233
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2316	3	-0.3396554215662704
2316	4	-0.18091493864380226
2316	5	-0.24975702971262592
2316	6	-0.09331818203169462
2316	7	-0.1328888996282629
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2316	12-13	-0.5511612271668938
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2316	54-55	-0.7387788202522785
2316	56-57	-0.74036593346937
2316	58-59	-0.930262551620082
2316	60-61	-0.7016684379663616
2316	62-63	-0.36315381364824617
2316	64-65	-0.5179198669028864
2316	66-67	-0.5429358963704871
2316	68-69	-0.30668612500542025
2316	70-71	-0.20683999310276135
2316	72-73	-0.552358158693707
2316	74-75	-0.45910429373285666
2316	76-77	-0.9212776399111107
2316	78-79	-0.7607033553385847
2316	80-81	-0.2787060380287869
2316	82-83	-0.2886149557595523
2316	84-85	-0.2455390931655863
2316	86-87	-0.3032534184890281
2316	88-89	-0.46333033290284575
2316	90-91	-0.21210407787869912
2316	92-93	-0.17380629266926206
2316	94-95	-0.21200193840218517
2316	96-97	-0.0576400872507854
2316	98-99	-0.05273110106389112
2316	100-101	0.136681686773926
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	1.0
5	2.0
6	6.0
7	18.0
8	47.0
9	110.0
10	204.0
11	336.0
12	572.0
13	813.0
14	1003.0
15	1424.0
16	2146.0
17	3240.0
18	3324.0
19	1813.0
20	1171.0
21	1232.0
22	1549.0
23	1887.0
24	2405.0
25	2780.0
26	1812.0
27	1376.0
28	1677.0
29	2001.0
30	2585.0
31	3442.0
32	4313.0
33	5715.0
34	7882.0
35	12553.0
36	20292.0
37	46133.0
38	96963.0
39	34942.0
40	26.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.97796451914099	22.288702147525676	15.527917833800187	22.205415499533146
2	15.310477712595736	22.824004749866504	40.99224412521425	20.873273412323513
3	17.04744051264433	28.491567956741896	34.1288631305361	20.33212840007767
4	12.088162460361628	22.051446777199672	38.605999260457246	27.254391501981452
5	11.469508620335553	38.516488235491806	36.38054197769206	13.633461166480581
6	24.77510950458743	39.715530793847584	20.168261780378423	15.34109792118657
7	21.913240252878115	35.927213523676514	24.245977363450674	17.913568859994697
8	22.87852365784531	34.860360646310454	23.06261832656826	19.198497369275984
9	24.627983136480243	17.015873964234103	23.090624614913537	35.265518284372114
10-11	21.71682281728324	28.292699320754156	30.11142768589641	19.879050176066198
12-13	23.580921369544843	27.112887747062143	29.527589928191876	19.778600955201142
14-15	21.52754050183628	28.537154145031767	28.0037267103939	21.93157864273805
16-17	19.136688766946296	31.339515934793283	29.416586225631967	20.10720907262845
18-19	18.893975660668342	28.493597762484267	32.88386352348981	19.728563053357583
20-21	20.203362995104502	27.268976127439814	32.83419903882419	19.693461838631503
22-23	19.8855849766801	28.292139194987246	32.205551219767216	19.616724608565445
24-25	19.675164396912585	28.722689201148633	32.825423735142664	18.77672266679612
26-27	19.25622627629562	28.577296566609345	33.49819732520776	18.668279831887272
28-29	18.879127650238534	27.77758068581778	33.962077432244456	19.381214231699236
30-31	18.852188135393853	27.23243208929168	34.19704887853277	19.718330896781698
32-33	18.58123877414609	28.34236380541978	33.4774101278207	19.598987292613433
34-35	19.321269249657817	28.13535774906681	33.289700464400624	19.253672536874753
36-37	18.808080317715792	28.53075117677702	33.44418034516303	19.216988160344155
38-39	18.534001501137055	28.398563090699298	33.80937799900671	19.258057409156937
40-41	18.686729126913296	27.961664992512986	34.01998528736319	19.33162059321053
42-43	19.512727924509985	27.86588348637214	33.25970791308342	19.36168067603446
44-45	19.00824131711707	28.354313155113765	33.04984745908281	19.587598068686354
46-47	19.7474206208434	28.12372804773765	32.10024757558897	20.028603755829977
48-49	19.425908430639627	28.475860446532263	31.76286515532287	20.335365967505236
50-51	19.005253979693574	28.407711811558755	32.18968098970489	20.39735321904278
52-53	19.366908516525577	28.888486428152664	32.416345216712664	19.328259838609096
54-55	19.581623393839365	28.928628774780897	31.357707517261208	20.13204031411853
56-57	19.851043887720923	29.661833403660236	30.41296205708055	20.074160651538293
58-59	18.844194834874305	30.032748808794757	31.64367652989589	19.479379826435046
60-61	19.818364797723582	30.753446270298124	30.282367307157298	19.14582162482099
62-63	18.96167965671904	31.204321635439502	30.546851950748593	19.28714675709287
64-65	19.103517295204096	31.32386824053086	30.13562815777976	19.43698630648528
66-67	19.11339651811521	31.147819527533176	29.88972537772699	19.849058576624618
68-69	19.087451477565317	30.97929896168058	30.13964378604757	19.79360577470653
70-71	19.07272700115013	30.74877892126843	30.362477408176375	19.81601666940507
72-73	19.24979320672419	30.290220125140273	30.303103859705466	20.156882808430073
74-75	19.151836312474675	30.54240725076883	30.00838379481549	20.29737264194101
76-77	18.88864616183173	30.641676982566597	30.259848650344207	20.20982820525747
78-79	19.260965923624557	30.43964517701007	29.950279224258807	20.349109675106565
80-81	19.197130448096736	30.316830795774095	29.927701034816284	20.558337721312885
82-83	19.316656178868925	30.34303501306151	29.784909895190488	20.555398912879077
84-85	19.196615283503615	30.094812391707	29.631579733524525	21.07699259126486
86-87	19.123654749546294	30.252171445215353	29.830018596383788	20.794155208854566
88-89	19.445922556866872	29.981721195011886	29.737505904670154	20.83485034345109
90-91	19.258057409156937	29.841447066247937	29.999589241104268	20.90090628349085
92-93	19.441218534935047	29.627852440467965	29.942829830057843	20.98809919453915
94-95	19.45914255947602	29.669115038630007	29.70197575028847	21.169766651605507
96-97	19.52602069108446	29.344670300022592	29.76327173805483	21.366037270838117
98-99	19.44088141555248	29.419167166113073	29.547062788348615	21.59288862998583
100-101	19.12361904370702	29.58593249674657	29.57715711114306	21.713291348403345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	750.0
1	449.0
2	157.0
3	156.0
4	111.0
5	101.0
6	177.0
7	320.5
8	480.0
9	549.0
10	461.0
11	345.5
12	321.0
13	338.0
14	356.5
15	394.5
16	452.5
17	459.0
18	489.5
19	549.0
20	601.0
21	664.0
22	721.0
23	834.0
24	1005.5
25	1254.5
26	1697.5
27	2373.5
28	3059.5
29	3840.0
30	5048.0
31	6213.0
32	7493.0
33	9127.0
34	10627.0
35	11821.5
36	13157.5
37	14008.5
38	13446.5
39	12834.0
40	12156.0
41	11332.5
42	10825.0
43	10663.0
44	10658.5
45	10325.0
46	9700.0
47	8834.5
48	8343.0
49	7747.5
50	6892.0
51	6184.5
52	5295.5
53	4367.0
54	3582.5
55	2954.5
56	2473.0
57	1985.5
58	1556.0
59	1271.0
60	1018.5
61	764.5
62	553.5
63	369.5
64	253.0
65	196.5
66	143.5
67	119.5
68	94.5
69	64.0
70	51.5
71	39.5
72	29.0
73	19.0
74	16.0
75	16.5
76	10.0
77	5.0
78	4.5
79	4.0
80	4.0
81	3.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017550607363039913
2	0.0
3	0.003360754601433175
4	0.023898699387969246
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.8670858896850973E-4
16-17	9.335429448425486E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.8670858896850973E-4
28-29	0.005414549080086782
30-31	1.8670858896850973E-4
32-33	0.0
34-35	0.012136058282953131
36-37	0.003920880368338704
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	3.7341717793701947E-4
60-61	0.002800628834527646
62-63	0.010455680982236546
64-65	0.0022405030676221167
66-67	0.004107588957307214
68-69	0.0031740460124646654
70-71	3.7341717793701947E-4
72-73	0.00653480061389784
74-75	0.006908217791834861
76-77	0.0024272116565906264
78-79	5.601257669055292E-4
80-81	0.007468343558740389
82-83	0.009522138037393997
84-85	0.0018670858896850972
86-87	0.0011202515338110583
88-89	1.8670858896850973E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	1.8670858896850973E-4
98-99	1.8670858896850973E-4
100-101	9.335429448425486E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	267797.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.255086012157456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.21469835512572	36.16820015852627
2	17.819642414396032	18.623338941403077
3	6.211784580695434	9.737920126596999
4	2.5543009363579454	5.33900860521275
5	1.23738319030607	3.232978251972074
6	0.6786763291354175	2.1278573972031913
7	0.4117446625877954	1.5061026931000399
8	0.2858299105592142	1.1948853248895208
9	0.2383808205413724	1.1210949252934275
>10	1.236998076726616	11.989578704575216
>50	0.08399743284089338	2.970088316046548
>100	0.02081987651611887	1.739960794470619
>500	0.0028717071056715685	1.110078480129056
>1k	0.0028717071056715685	3.1389072805812135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	3243	1.2109919080497542	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1945	0.7262964110875028	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1914	0.7147204785714553	No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT	1265	0.4723727300903296	RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.3360754601433175	No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT	871	0.32524636198314394	RNA PCR Primer, Index 31 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	623	0.23263890185476313	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	565	0.21098070553441597	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	468	0.1747592392745251	No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT	389	0.14525928221750056	RNA PCR Primer, Index 31 (96% over 27bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	3.7341717793701947E-4	0.0
20-21	0.0	0.0	0.0	7.468343558740389E-4	0.0
22-23	0.0	0.0	0.0	0.0011202515338110583	0.0
24-25	0.0	0.0	0.0	0.0022405030676221167	0.0
26-27	0.0	0.0	0.0	0.005041131902149763	0.0
28-29	0.0	0.0	0.0	0.009335429448425487	0.0
30-31	0.0	0.0	0.0	0.016617064418197366	0.0
32-33	0.0	0.0	0.0	0.029686665645993048	0.0
34-35	0.0	0.0	0.0	0.047984107364907005	0.0
36-37	0.0	0.0	0.0	0.0653480061389784	0.0
38-39	0.0	0.0	0.0	0.07916444172264814	0.0
40-41	0.0	0.0	0.0	0.09372771166219188	0.0
42-43	0.0	0.0	0.0	0.11295869632594838	0.0
44-45	0.0	0.0	0.0	0.15478142025489455	0.0
46-47	0.0	0.0	0.0	0.20463261350948667	0.0
48-49	0.0	0.0	0.0	0.2473888803832754	0.0
50-51	0.0	0.0	0.0	0.28305022087626075	0.0
52-53	0.0	0.0	0.0	0.3237526932713959	0.0
54-55	0.0	0.0	0.0	0.3672557945010586	0.0
56-57	0.0	3.7341717793701947E-4	0.0	0.4355911380635332	0.0
58-59	0.0	3.7341717793701947E-4	0.0	0.5009391442025116	0.0
60-61	0.0	3.7341717793701947E-4	0.0	0.564793481629742	0.0
62-63	0.0	3.7341717793701947E-4	0.0	0.6398503343950829	0.0
64-65	0.0	3.7341717793701947E-4	0.0	0.7378723436035505	0.0
66-67	0.0	3.7341717793701947E-4	0.0	0.8528848344081524	0.0
68-69	0.0	3.7341717793701947E-4	0.0	0.9847010982199202	0.0
70-71	0.0	3.7341717793701947E-4	0.0	1.1170774877985936	0.0
72-73	0.0	3.7341717793701947E-4	0.0	1.2649506902616534	0.0
74-75	0.0	3.7341717793701947E-4	0.0	1.4359757577568084	0.0
76-77	0.0	3.7341717793701947E-4	0.0	1.6256716841488141	0.0
78-79	0.0	3.7341717793701947E-4	0.0	1.8430004817081596	0.0
80-81	0.0	3.7341717793701947E-4	0.0	2.054914730187418	0.0
82-83	0.0	3.7341717793701947E-4	0.0	2.266268852899771	0.0
84-85	0.0	3.7341717793701947E-4	0.0	2.5170184878844797	0.0
86-87	0.0	3.7341717793701947E-4	0.0	2.8144452701113156	0.0
88-89	0.0	3.7341717793701947E-4	0.0	3.112245469516089	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1005	0.0	53.412476	1
GTTTCGA	40	5.378488E-4	47.50402	1
GTATCAA	1720	0.0	39.218433	1
TGGTATC	510	0.0	33.532246	2
GTGGTAT	520	0.0	32.887394	1
ATCAACG	2085	0.0	32.12502	3
TATCAAC	2115	0.0	31.89395	2
CGCTAGA	30	0.003943841	31.67526	62-63
CGGAGAT	75	3.0706663E-4	31.669342	1
TCAACGC	2130	0.0	31.446321	4
CAACGCA	2170	0.0	30.860899	5
AACGCAG	2210	0.0	30.302332	6
ACGCAGA	2500	0.0	26.787262	7
CGCAGAG	2510	0.0	26.680538	8
GTATAGT	125	7.3584015E-6	26.602247	1
CATATAG	90	8.925149E-4	26.391119	3
CGAAATC	45	8.9466193E-4	26.386192	12-13
GAGTACT	1575	0.0	25.481524	12-13
GCAGAGT	2635	0.0	24.69387	9
TATACAC	1145	0.0	24.47805	3
>>END_MODULE