FastQCFastQC Report
Fri 10 Feb 2017
SRR2031723_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031723_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110203
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.9346388029363992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.8475268368374725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.4673194014681996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.4092447574022486No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.2604284819832491No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.2540765677885357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2460.22322441312849925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1330.12068636969955447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7850.035.0908431
GTATATA753.0629383E-431.6624281
GTATCAA11400.030.4125961
ATGGGAT1405.733E-727.1392215
TAGTATG700.00836469327.1392217
TCAACGC13000.026.3041734
ATCAACG13050.026.2033883
TATCAAC13400.025.8734042
GAGTACT8300.025.76126512-13
CAACGCA13300.025.7108445
AACGCAG13400.025.5189726
CATGGGA2900.024.5656784
GTACATG6200.024.512851
GTACTTT8800.024.02758214-15
CCAACAC1000.001645480423.7468223
ACGCAGA14650.023.6657757
TACATGG6050.023.5505682
CGCAGAG14700.023.2727628
ACATGGG5950.022.3499493
GCAGAGT15250.022.1218419
Produced by FastQC (version 0.11.5)