##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031723_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 110203 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.873533388383258 33.0 31.0 34.0 28.0 34.0 2 31.185049408818273 33.0 31.0 34.0 30.0 34.0 3 31.257098264112592 34.0 31.0 34.0 30.0 34.0 4 34.577443445278256 37.0 35.0 37.0 33.0 37.0 5 34.56551092075534 37.0 35.0 37.0 33.0 37.0 6 34.57977550520403 37.0 37.0 37.0 33.0 37.0 7 34.55871437256699 37.0 36.0 37.0 33.0 37.0 8 34.54631906572416 37.0 37.0 37.0 33.0 37.0 9 36.24367757683547 39.0 38.0 39.0 33.0 39.0 10-11 36.15551300781286 39.0 38.0 39.0 33.0 39.0 12-13 36.07901781258224 39.0 38.0 39.0 32.0 39.0 14-15 37.322305200402894 41.0 38.0 41.0 32.5 41.0 16-17 37.238636879213814 41.0 38.0 41.0 32.0 41.0 18-19 37.263472863715144 41.0 38.5 41.0 32.0 41.0 20-21 37.282043138571545 41.0 39.0 41.0 32.0 41.0 22-23 37.16499550828925 41.0 38.5 41.0 32.0 41.0 24-25 37.06020707240275 41.0 38.0 41.0 32.0 41.0 26-27 36.9083963231491 40.0 38.0 41.0 32.0 41.0 28-29 36.761766921045705 40.0 38.0 41.0 31.0 41.0 30-31 36.515394317759046 40.0 37.5 41.0 30.5 41.0 32-33 36.18551219113817 40.0 36.5 41.0 30.0 41.0 34-35 35.94599511810023 40.0 36.0 41.0 29.5 41.0 36-37 35.76524232552653 40.0 35.5 41.0 27.5 41.0 38-39 35.512953367875646 40.0 35.0 41.0 25.5 41.0 40-41 35.366423781566745 40.0 35.0 41.0 24.5 41.0 42-43 35.175399036323874 40.0 35.0 41.0 21.5 41.0 44-45 34.87355153670953 40.0 35.0 41.0 12.0 41.0 46-47 34.495848570365595 40.0 35.0 41.0 3.5 41.0 48-49 34.2405606017985 40.0 35.0 41.0 2.0 41.0 50-51 33.622514813571314 39.5 35.0 40.5 2.0 41.0 52-53 33.602474524287 39.5 35.0 40.5 2.0 41.0 54-55 33.76988829705181 40.0 35.0 41.0 2.0 41.0 56-57 33.62115368910102 40.0 35.0 41.0 2.0 41.0 58-59 33.43536473598722 40.0 35.0 41.0 2.0 41.0 60-61 33.19974047893433 39.0 35.0 41.0 2.0 41.0 62-63 32.914984165585324 39.0 35.0 41.0 2.0 41.0 64-65 32.66546282769072 39.0 34.5 41.0 2.0 41.0 66-67 32.37256699000935 38.0 34.0 41.0 2.0 41.0 68-69 32.01818462292315 37.0 34.0 40.0 2.0 41.0 70-71 31.654179105831965 37.0 34.0 39.5 2.0 41.0 72-73 31.260959320526666 36.0 33.0 39.0 2.0 41.0 74-75 30.84256326960246 36.0 33.0 39.0 2.0 41.0 76-77 30.454352422347846 35.0 32.5 37.5 2.0 40.0 78-79 30.05206301098881 35.0 32.0 37.0 2.0 39.0 80-81 29.682354382367087 35.0 32.0 37.0 2.0 39.0 82-83 29.325290600074407 35.0 31.5 36.0 2.0 38.5 84-85 28.985966806711254 35.0 31.0 36.0 2.0 37.0 86-87 28.719000390189017 35.0 31.0 35.5 2.0 37.0 88-89 28.433395642586863 35.0 31.0 35.0 2.0 36.5 90-91 28.18794860394 35.0 30.0 35.0 2.0 36.0 92-93 27.951920546627587 35.0 30.0 35.0 2.0 36.0 94-95 27.717167409235685 35.0 29.0 35.0 2.0 36.0 96-97 27.46650726386759 35.0 29.0 35.0 2.0 35.0 98-99 27.25107301979075 35.0 29.0 35.0 2.0 35.0 100-101 26.761671642332786 34.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15297886203913436 1101 2 -0.0541874275825478 1101 3 0.0162423210353424 1101 4 0.3240409634775858 1101 5 -0.2664217390652581 1101 6 0.09919504695887582 1101 7 -0.6082875768828657 1101 8 -0.41216466182719813 1101 9 -0.15476058955714933 1101 10-11 -0.8382383300484619 1101 12-13 -0.5005751725946297 1101 14-15 -0.43332245026234517 1101 16-17 -0.6356060870069484 1101 18-19 -0.5871126786553234 1101 20-21 -0.6526378035374094 1101 22-23 -0.3464828757324554 1101 24-25 -0.3893548059294929 1101 26-27 -1.210709356701578 1101 28-29 -1.2398779663297077 1101 30-31 -0.850109174480572 1101 32-33 -0.9166440956939468 1101 34-35 -1.1827478588804965 1101 36-37 -1.226504999840408 1101 38-39 -0.7058577496813001 1101 40-41 -1.0023681232356267 1101 42-43 -0.5917826409308518 1101 44-45 -0.41141381139357946 1101 46-47 -0.506273266207323 1101 48-49 0.10007719239590074 1101 50-51 -0.24134441208109791 1101 52-53 0.07843474357256497 1101 54-55 -0.19020375647528454 1101 56-57 -0.4639504259414764 1101 58-59 -0.9351208235928681 1101 60-61 -1.0865897657163934 1101 62-63 -0.5870407826749684 1101 64-65 -0.28201223808756737 1101 66-67 -0.16417894399581456 1101 68-69 -0.5611962462888869 1101 70-71 -0.3789254058346003 1101 72-73 -0.22189976124785105 1101 74-75 -0.42727930054194374 1101 76-77 -0.8788595355614888 1101 78-79 -0.8855163448418324 1101 80-81 -0.15208008526019512 1101 82-83 -0.6347378775391661 1101 84-85 -0.6318025892138408 1101 86-87 -1.328486933208648 1101 88-89 -1.4883067762221174 1101 90-91 -2.069293687515298 1101 92-93 -2.473293258449644 1101 94-95 -2.591002045570697 1101 96-97 -2.721633482005412 1101 98-99 -3.2448005082167057 1101 100-101 -3.1354042301489393 1102 1 0.6009112146396198 1102 2 0.5428240400416868 1102 3 0.5478072463220336 1102 4 1.1691701190434358 1102 5 0.6278212819008075 1102 6 0.7032724244638047 1102 7 0.407465927113428 1102 8 0.7724756717764194 1102 9 0.9501855473593324 1102 10-11 1.0982263983194258 1102 12-13 0.9258119465991612 1102 14-15 1.0453639855073718 1102 16-17 1.1773789703311763 1102 18-19 1.0024274580294943 1102 20-21 0.887209294806155 1102 22-23 1.3453392023400497 1102 24-25 0.8763697394197507 1102 26-27 1.3057391548239679 1102 28-29 1.1156183848807686 1102 30-31 0.8129280084222401 1102 32-33 1.0019241887957975 1102 34-35 1.155199552216466 1102 36-37 0.7846151206275636 1102 38-39 1.155546165527781 1102 40-41 1.4177060109008295 1102 42-43 1.2605861763980002 1102 44-45 1.0977950383689645 1102 46-47 1.4120633491060133 1102 48-49 0.8448935945735911 1102 50-51 1.018530486563641 1102 52-53 1.1815854443718266 1102 54-55 1.0646323375823954 1102 56-57 0.7422351410688321 1102 58-59 0.6542455612096276 1102 60-61 0.14670109493459194 1102 62-63 0.11972395727638485 1102 64-65 0.5892955344132957 1102 66-67 0.5049974720820316 1102 68-69 0.4404833552401328 1102 70-71 0.507788366897941 1102 72-73 0.8117501866265862 1102 74-75 0.7786745972919498 1102 76-77 0.4270596118959382 1102 78-79 0.4312333366130474 1102 80-81 0.4484643373043866 1102 82-83 0.47489956019974144 1102 84-85 0.6855841824761875 1102 86-87 0.8605551147468695 1102 88-89 0.40605207933215226 1102 90-91 0.06565360774706619 1102 92-93 0.19148144327629169 1102 94-95 -0.26023637894263985 1102 96-97 -0.14002801461284875 1102 98-99 -0.09919643083919993 1102 100-101 -0.5309793488793879 1103 1 0.1982890721026429 1103 2 0.5324027043177963 1103 3 0.7495105183973365 1103 4 0.29949284427875966 1103 5 0.3806227162351803 1103 6 -0.5274537073156722 1103 7 0.039245398202631065 1103 8 0.2722515570296693 1103 9 -0.04533215770565846 1103 10-11 -0.035233933370399484 1103 12-13 -0.06976178715252246 1103 14-15 0.3734679747498646 1103 16-17 0.33868555930472155 1103 18-19 -0.621003738743255 1103 20-21 -1.104274344817334 1103 22-23 -0.6945532225815114 1103 24-25 -0.30874904139602677 1103 26-27 -0.4514885457587283 1103 28-29 -0.668615142685347 1103 30-31 0.07508623343344567 1103 32-33 -0.6268297332122685 1103 34-35 -0.5824181080255855 1103 36-37 -0.7601518000358141 1103 38-39 -0.39992223429293006 1103 40-41 -0.7214692468311341 1103 42-43 -0.2595281221228376 1103 44-45 -0.6068217254140933 1103 46-47 -0.39318093596794057 1103 48-49 -0.8092413225039508 1103 50-51 -1.386556918187594 1103 52-53 -0.9996673570625063 1103 54-55 -0.9590117681997654 1103 56-57 -1.224819991158867 1103 58-59 -1.0281838426451415 1103 60-61 -0.8720685151057452 1103 62-63 -0.6669748325039819 1103 64-65 -1.0815359312971466 1103 66-67 -1.4960670729650367 1103 68-69 -1.4892006429669493 1103 70-71 -0.7307817810177966 1103 72-73 -1.2731332737051027 1103 74-75 -0.8811752458277304 1103 76-77 -1.408720307422751 1103 78-79 -1.2106873267666032 1103 80-81 -1.1286311355777343 1103 82-83 -1.416796988433159 1103 84-85 -1.2074010259505243 1103 86-87 -0.9276564495740622 1103 88-89 -0.89420565262661 1103 90-91 -0.39501425419825154 1103 92-93 -2.092247826109631 1103 94-95 -1.8984031203142244 1103 96-97 -1.2946671898705802 1103 98-99 -1.5282641134927175 1103 100-101 -1.2361003708426352 1104 1 -0.03326560510420862 1104 2 0.2679099506946052 1104 3 0.09909330276272854 1104 4 0.35911076259233 1104 5 0.2833455972189327 1104 6 0.23550193607475478 1104 7 -0.013235936887404876 1104 8 0.2533670598842974 1104 9 -0.11208665924277028 1104 10-11 0.3124823599980644 1104 12-13 0.1756817789432219 1104 14-15 0.586028361223299 1104 16-17 -0.05025822637819033 1104 18-19 -0.060781955695382806 1104 20-21 0.037578970949027735 1104 22-23 -0.19235735082042282 1104 24-25 -0.25582853195377453 1104 26-27 0.08216318897995478 1104 28-29 -0.2413533947714228 1104 30-31 -0.18957121057182036 1104 32-33 -0.11991273716483875 1104 34-35 -0.2726354993299296 1104 36-37 -0.22803717552989156 1104 38-39 -0.05615851009441997 1104 40-41 0.1926721673102776 1104 42-43 0.13270947120170717 1104 44-45 0.030420259653979542 1104 46-47 -0.012855946947297525 1104 48-49 0.3557235435478674 1104 50-51 0.7044619663095517 1104 52-53 0.5115864857130745 1104 54-55 0.6168556011458648 1104 56-57 0.4552414932504405 1104 58-59 0.2816536628445405 1104 60-61 0.6940821216970647 1104 62-63 -0.07370517945171429 1104 64-65 0.2497662206571789 1104 66-67 0.45589823226114135 1104 68-69 0.1315174070989258 1104 70-71 0.4671760582443909 1104 72-73 0.8433028264266476 1104 74-75 0.7492046399869388 1104 76-77 0.5286424505921836 1104 78-79 0.11732101754608948 1104 80-81 -0.03903956772529682 1104 82-83 -0.04212636919731949 1104 84-85 0.4993403003560175 1104 86-87 0.5192034538075809 1104 88-89 0.705541822120864 1104 90-91 0.7954776210762375 1104 92-93 0.5353323232096479 1104 94-95 0.5173851976480073 1104 96-97 0.6144237192203299 1104 98-99 0.42090145523806655 1104 100-101 0.5644595764564428 1105 1 0.7191586373200352 1105 2 0.5345766173612745 1105 3 0.6664670401364639 1105 4 1.051231974713538 1105 5 0.9462748901482243 1105 6 0.858633249206072 1105 7 0.28272365907219665 1105 8 0.3065993831166196 1105 9 0.35771132055521093 1105 10-11 0.1738965014122087 1105 12-13 0.5243686476300908 1105 14-15 0.32325058344552104 1105 16-17 0.24933773321777153 1105 18-19 0.21856147864804853 1105 20-21 0.6505082638783151 1105 22-23 0.15435982089675093 1105 24-25 0.28755530643005756 1105 26-27 0.3437288455456198 1105 28-29 0.25551529209726453 1105 30-31 0.459216668216051 1105 32-33 0.7359963537442553 1105 34-35 0.6423645006700696 1105 36-37 0.03332646083374868 1105 38-39 0.726164722228809 1105 40-41 0.818530753168865 1105 42-43 0.8056892691815065 1105 44-45 0.7727434919772165 1105 46-47 1.4735581944668397 1105 48-49 2.000976068800398 1105 50-51 1.9171387339863202 1105 52-53 1.721636990763578 1105 54-55 1.6496838839741486 1105 56-57 1.4960495740585245 1105 58-59 1.5959465921374658 1105 60-61 2.13706191967686 1105 62-63 2.1949816892351564 1105 64-65 2.0475945034854632 1105 66-67 2.1445851009480066 1105 68-69 2.161668922250442 1105 70-71 1.8374790885474255 1105 72-73 2.0086058567296767 1105 74-75 2.0299117106940123 1105 76-77 2.3304101273598583 1105 78-79 2.067573542798616 1105 80-81 2.027021038335313 1105 82-83 2.3221160550451003 1105 84-85 2.1765120175277346 1105 86-87 2.1386478982520245 1105 88-89 2.225794347373391 1105 90-91 2.211942267540884 1105 92-93 2.532099999977323 1105 94-95 2.312031662294469 1105 96-97 2.575332810129421 1105 98-99 2.330648929985543 1105 100-101 2.5732474552443207 1106 1 -0.3581490549876598 1106 2 -0.08465415186949699 1106 3 -0.2116098829404578 1106 4 -0.19799879451722546 1106 5 0.04742873630207356 1106 6 -0.03790521233239019 1106 7 -0.465353264004726 1106 8 -0.11570830919107067 1106 9 0.26194208978597544 1106 10-11 -0.09418042166470997 1106 12-13 -0.027746736985299947 1106 14-15 0.4669044295993672 1106 16-17 0.5343377332177681 1106 18-19 0.09952301710958977 1106 20-21 0.2632005715706285 1106 22-23 0.20051366705059337 1106 24-25 0.6065937679685192 1106 26-27 0.229113460930229 1106 28-29 0.6426306767126491 1106 30-31 0.09787051436990168 1106 32-33 0.523880969128868 1106 34-35 0.9004414237469902 1106 36-37 0.7592879992952817 1106 38-39 0.8636647222288119 1106 40-41 0.9900692147073329 1106 42-43 0.9835738845661197 1106 44-45 1.445435799669518 1106 46-47 1.5468274252360672 1106 48-49 1.7800145303388604 1106 50-51 1.7990618109094 1106 52-53 1.5118292984558863 1106 54-55 1.8227608070510684 1106 56-57 1.7956649586739104 1106 58-59 2.047485053675935 1106 60-61 1.828600381215324 1106 62-63 2.2180586123120705 1106 64-65 2.0902868111777693 1106 66-67 2.1034312547941596 1106 68-69 1.9351304607119815 1106 70-71 2.1897867808551155 1106 72-73 2.541875087498905 1106 74-75 2.353565556847858 1106 76-77 2.3929101273598583 1106 78-79 2.4069966197216957 1106 80-81 2.403559499873772 1106 82-83 2.636923747352796 1106 84-85 2.6492043252200403 1106 86-87 2.324224821328947 1106 88-89 1.9086789627580032 1106 90-91 2.129442267540881 1106 92-93 1.7120999999773225 1106 94-95 1.857031662294471 1106 96-97 2.1236020408986533 1106 98-99 2.0543027761393873 1106 100-101 1.8615166860135517 1107 1 0.34915863732003416 1107 2 0.18457661736127307 1107 3 0.326467040136464 1107 4 0.811231974713543 1107 5 0.34627489014823 1107 6 0.5286332492060666 1107 7 0.36272365907219495 1107 8 0.026599383116625575 1107 9 0.4277113205552112 1107 10-11 0.2288965014122084 1107 12-13 0.084368647630086 1107 14-15 -0.02174941655447782 1107 16-17 0.4443377332177647 1107 18-19 0.8535614786480537 1107 20-21 0.6955082638783168 1107 22-23 0.8143598208967475 1107 24-25 0.5425553064300601 1107 26-27 0.6837288455456161 1107 28-29 0.530515292097256 1107 30-31 0.49921666821605726 1107 32-33 0.36599635374425077 1107 34-35 0.7523645006700761 1107 36-37 0.613326460833747 1107 38-39 0.8911647222288153 1107 40-41 0.7685307531688608 1107 42-43 0.5206892691815099 1107 44-45 0.15274349197721193 1107 46-47 -0.9164418055331609 1107 48-49 -2.0240239311996078 1107 50-51 -1.40286126601368 1107 52-53 -2.1733630092364216 1107 54-55 -2.1403161160258506 1107 56-57 -2.1689504259414747 1107 58-59 -2.019053407862529 1107 60-61 -1.6779380803231376 1107 62-63 -1.5350183107648476 1107 64-65 -1.4574054965145393 1107 66-67 -1.3154148990519943 1107 68-69 -1.2833310777495583 1107 70-71 -1.4375209114525802 1107 72-73 -1.5513941432703184 1107 74-75 -1.1500882893059874 1107 76-77 -1.0395898726401427 1107 78-79 -1.332426457201386 1107 80-81 -0.9479789616646883 1107 82-83 -0.762883944954897 1107 84-85 -0.583487982472267 1107 86-87 -1.3013521017479768 1107 88-89 -1.8392056526266103 1107 90-91 -1.4580577324591175 1107 92-93 -1.0129000000226789 1107 94-95 -0.7679683377055291 1107 96-97 -0.7146671898705819 1107 98-99 -0.624351070014459 1107 100-101 -0.6717525447556802 1108 1 -0.3083852223290897 1108 2 -0.3285812773755694 1108 3 -0.33897155635476395 1108 4 -0.26210135861979467 1108 5 -0.12547949581668405 1108 6 0.11880868780256293 1108 7 0.10903944854587877 1108 8 0.05905552346749943 1108 9 0.0782376363446815 1108 10-11 0.1161772031665933 1108 12-13 -0.08721029973833794 1108 14-15 -0.19613538146676035 1108 16-17 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-0.5255607348415019 2316 88-89 -0.8231624871589815 2316 90-91 -0.9861152864159521 2316 92-93 -1.1476122302385043 2316 94-95 -1.255378409647978 2316 96-97 -1.3738038805180608 2316 98-99 -1.5180201347626614 2316 100-101 -1.2617885159787008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4089.0 3 541.0 4 393.0 5 302.0 6 304.0 7 325.0 8 286.0 9 323.0 10 312.0 11 373.0 12 479.0 13 606.0 14 612.0 15 833.0 16 1106.0 17 1683.0 18 1680.0 19 827.0 20 639.0 21 521.0 22 483.0 23 526.0 24 499.0 25 568.0 26 574.0 27 682.0 28 733.0 29 880.0 30 1012.0 31 1329.0 32 1690.0 33 2305.0 34 3492.0 35 5943.0 36 9488.0 37 19747.0 38 32818.0 39 11177.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21389300351687 21.345680246453593 14.381912196363173 21.05851455366636 2 16.781780508197315 23.196044930749938 38.966338289287144 21.055836271765603 3 19.01836588180769 28.13860288437946 31.609127507020112 21.23390372679274 4 13.302556366379193 20.689028435372915 38.06741246285408 27.941002735393816 5 12.855974557019536 38.167196728759656 34.151749204906864 14.825079509313948 6 26.121837096261302 38.320083586971336 19.508472266388043 16.049607050379322 7 23.425134248616626 35.97045167504112 23.796759860799405 16.807654215542854 8 23.772360973571125 33.50352960415739 22.374147125893757 20.349962296377726 9 25.961389961389962 17.567113331819215 22.822620940268 33.64887576652282 10-11 23.217399992733352 28.032463757584562 28.59335828216401 20.156777967518074 12-13 25.46586108208786 27.312951437786765 28.309635215554447 18.911552264570936 14-15 22.72737599222126 28.462835151553705 28.27836264749849 20.53142620872655 16-17 19.653835842394884 30.806701534518723 29.291267738754662 20.24819488433173 18-19 19.306863208404522 28.85373877640045 32.6961176342288 19.143280380966228 20-21 21.416433426629347 27.34633419359529 32.559703391370725 18.67752898840464 22-23 20.98343078593593 27.794663504568234 32.17253426742448 19.049371442071354 24-25 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31.115816449398174 19.966739518631776 50-51 19.839438078708962 29.14603229411818 31.067414198871436 19.947115428301423 52-53 20.31334462912406 29.79953756910344 30.16793782166884 19.71917998010366 54-55 20.45230535835139 29.381679770713248 30.024391240955485 20.14162362997988 56-57 20.06014955614716 29.474645417457907 30.180630389154906 20.284574637240027 58-59 20.44089723905479 29.354689246032105 30.07740458431376 20.12700893059934 60-61 20.40083036935083 29.55306323616922 30.266236037484727 19.779870356995225 62-63 19.908877411500704 29.44631444106785 30.357086075868867 20.287722071562577 64-65 20.205737757058603 29.388511763583324 30.119135247130668 20.2866152322274 66-67 19.992639909861612 29.67570170735918 29.798369876332316 20.533288506446894 68-69 20.167108897935446 29.371274353009596 29.977827856935157 20.483788892119804 70-71 20.281651751237906 29.32585290510153 29.82419479398537 20.568300549675193 72-73 20.081591117652938 29.32101289285033 30.148281407583067 20.449114581913665 74-75 20.056502307328948 29.804058718796554 29.310799026198175 20.82863994767632 76-77 20.095409009336453 29.768519570205132 29.419140864587355 20.71693055587106 78-79 20.1685559164944 29.6085958974126 29.494105086208855 20.728743099884145 80-81 20.2058202139985 29.691724028260523 29.224198641495718 20.87825711624526 82-83 20.227823139451935 29.730147262623646 29.452979103338283 20.589050494586132 84-85 20.323020308027804 29.580209895052473 29.212212075780293 20.88455772113943 86-87 20.162470127486348 29.676695350337575 29.186922426874812 20.97391209530127 88-89 20.309930308839967 29.792289450012266 28.97634861935179 20.92143162179598 90-91 20.43068396065693 29.42870772096404 29.244712990936556 20.895895327442474 92-93 20.52990062659318 29.206330454064222 29.26948959237365 20.99427932696895 94-95 20.180531327227794 29.297953917720598 29.232537432766392 21.288977322285216 96-97 20.13681021443593 29.34742617835292 29.37649607783466 21.139267529376497 98-99 20.370841433944438 29.620982248305683 28.690244744444648 21.31793157330523 100-101 20.314104719750606 29.52957001463277 28.855190089705253 21.30113517591137 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 686.0 1 401.5 2 112.0 3 103.0 4 78.5 5 69.0 6 100.5 7 176.0 8 268.5 9 301.5 10 254.0 11 206.5 12 199.0 13 224.0 14 255.0 15 267.5 16 278.5 17 307.5 18 328.5 19 347.5 20 386.0 21 410.5 22 454.5 23 522.0 24 598.0 25 706.5 26 923.0 27 1191.5 28 1372.5 29 1575.5 30 1916.0 31 2283.5 32 2608.0 33 2971.0 34 3457.5 35 3819.0 36 4054.5 37 4333.0 38 4342.0 39 4166.5 40 4032.5 41 3964.0 42 3858.5 43 3861.5 44 3793.5 45 3728.0 46 3793.0 47 3827.0 48 4080.5 49 3899.0 50 3332.0 51 2910.0 52 2707.0 53 2533.5 54 2203.0 55 1919.5 56 1605.5 57 1326.5 58 1145.0 59 961.0 60 774.0 61 632.0 62 519.5 63 405.0 64 312.0 65 242.0 66 188.5 67 157.0 68 136.5 69 108.0 70 88.0 71 74.5 72 66.5 73 56.0 74 45.5 75 44.0 76 37.0 77 29.5 78 23.5 79 15.0 80 9.0 81 10.5 82 9.0 83 5.5 84 5.0 85 4.0 86 4.0 87 3.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14700144279193852 2 0.15153852435959095 3 0.14700144279193852 4 0.1488162754189995 5 0.13883469597016415 6 0.12522345126720688 7 0.13248278177545075 8 0.12159378601308495 9 0.11614928813190205 10-11 0.1007232108018838 12-13 0.12522345126720688 14-15 0.14473290200811229 16-17 0.15289964882988666 18-19 0.15153852435959095 20-21 0.14427919385134708 22-23 0.13565873887280744 24-25 0.12023266154278921 26-27 0.12522345126720688 28-29 0.12930682467809407 30-31 0.11705670444543252 32-33 0.11660299628866728 34-35 0.1338439062457465 36-37 0.1392884041269294 38-39 0.12159378601308495 40-41 0.12567715942397212 42-43 0.12204749416985018 44-45 0.14064952859722513 46-47 0.1361124470295727 48-49 0.14745515094870376 50-51 0.13792727965663368 52-53 0.12023266154278921 54-55 0.11115849840748436 56-57 0.13066794914838978 58-59 0.11977895338602397 60-61 0.11932524522925873 62-63 0.11932524522925873 64-65 0.14518661016487752 66-67 0.1361124470295727 68-69 0.14064952859722513 70-71 0.12522345126720688 72-73 0.12885311652132883 74-75 0.10798254131012766 76-77 0.13656615518633794 78-79 0.13656615518633794 80-81 0.14110323675399036 82-83 0.14654773463517326 84-85 0.13429761440251173 86-87 0.13792727965663368 88-89 0.13248278177545075 90-91 0.13202907361868552 92-93 0.14836256726223426 94-95 0.12613086758073735 96-97 0.11206591472101486 98-99 0.11705670444543252 100-101 0.1597052711813653 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 110203.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.07159514713756 #Duplication Level Percentage of deduplicated Percentage of total 1 80.37580450913612 56.320608331896594 2 13.155747788813924 18.436884658312387 3 3.5573224899962446 7.478017839804724 4 1.1939757319900026 3.346551364300428 5 0.550368423097344 1.92825966625228 6 0.3263361002836016 1.372013466058093 7 0.20849250851452325 1.022658185348856 8 0.12431851439375301 0.6968957287914122 9 0.08805894769557504 0.5553387838806566 >10 0.389790342005413 4.350153807065143 >50 0.015539814299219126 0.6978031451049427 >100 0.010359876199479416 1.5453299819424153 >500 0.002589969049869854 1.3148462383056723 >1k 0.001294984524934927 0.9346388029363992 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1030 0.9346388029363992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.8475268368374725 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 515 0.4673194014681996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 451 0.4092447574022486 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 0.2604284819832491 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.2540765677885357 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 246 0.22322441312849925 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 133 0.12068636969955447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.002722248940591454 0.0 4 0.0 0.0 0.0 0.002722248940591454 0.0 5 0.0 0.0 0.0 0.002722248940591454 0.0 6 0.0 0.0 0.0 0.002722248940591454 0.0 7 0.0 0.0 0.0 0.002722248940591454 0.0 8 0.0 0.0 0.0 0.002722248940591454 0.0 9 0.0 0.0 0.0 0.002722248940591454 0.0 10-11 0.0 0.0 0.0 0.002722248940591454 0.0 12-13 0.0 0.0 0.0 0.002722248940591454 0.0 14-15 0.0 0.0 0.0 0.002722248940591454 0.0 16-17 0.0 0.0 0.0 0.002722248940591454 0.0 18-19 0.0 0.0 0.0 0.002722248940591454 0.0 20-21 0.0 0.0 0.0 0.002722248940591454 0.0 22-23 0.0 0.0 0.0 0.0036296652541219386 0.0 24-25 0.0 0.0 0.0 0.005444497881182908 0.0 26-27 0.0 0.0 0.0 0.008166746821774361 0.0 28-29 0.0 0.0 0.0 0.012250120232661543 0.0 30-31 0.0 0.0 0.0 0.024500240465323085 0.0 32-33 0.0 0.0 0.0 0.05489868696859432 0.0 34-35 0.0 0.0 0.0 0.11206591472101485 0.0 36-37 0.0 0.0 0.0 0.17331651588432256 0.0 38-39 0.0 0.0 0.0 0.24091903124234365 0.0 40-41 0.0 0.0 0.0 0.2908269284865203 0.0 42-43 0.0 0.0 0.0 0.3448181991415842 0.0 44-45 0.0 0.0 0.0 0.4133281308131358 0.0 46-47 0.0 0.0 0.0 0.5045234703229494 0.0 48-49 0.0 0.0 0.0 0.5870983548542236 0.0 50-51 0.0 0.0 0.0 0.6528860375851837 0.0 52-53 0.0 0.0 0.0 0.726386758981153 0.0 54-55 0.0 0.0 0.0 0.808961643512427 0.0 56-57 0.0 0.0 0.0 0.9278331805849205 0.0 58-59 0.0 0.0 0.0 1.0435287605600574 0.0 60-61 0.0 0.0 0.0 1.1397148897942886 0.0 62-63 0.0 0.0 0.0 1.2535956371423644 0.0 64-65 0.0 0.0 0.0 1.3788190884095715 0.0 66-67 0.0 0.0 0.0 1.5190149088500313 0.0 68-69 0.0 0.0 0.0 1.6877943431667015 0.0 70-71 0.0 0.0 0.0 1.8416014083101184 0.0 72-73 0.0 0.0 0.0 2.0040289284320756 0.0 74-75 0.0 0.0 0.0 2.1750769035325717 0.0 76-77 0.0 0.0 0.0 2.3597361233360252 0.0 78-79 0.0 0.0 0.0 2.5911272832862986 0.0 80-81 0.0 0.0 0.0 2.8202499024527463 0.0 82-83 0.0 0.0 0.0 3.0575392684409683 0.0 84-85 0.0 0.0 0.0 3.3311252869704093 0.0 86-87 0.0 0.0 0.0 3.6292115459651733 0.0 88-89 0.0 0.0 0.0 3.9794742429879406 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 785 0.0 35.090843 1 GTATATA 75 3.0629383E-4 31.662428 1 GTATCAA 1140 0.0 30.412596 1 ATGGGAT 140 5.733E-7 27.139221 5 TAGTATG 70 0.008364693 27.139221 7 TCAACGC 1300 0.0 26.304173 4 ATCAACG 1305 0.0 26.203388 3 TATCAAC 1340 0.0 25.873404 2 GAGTACT 830 0.0 25.761265 12-13 CAACGCA 1330 0.0 25.710844 5 AACGCAG 1340 0.0 25.518972 6 CATGGGA 290 0.0 24.565678 4 GTACATG 620 0.0 24.51285 1 GTACTTT 880 0.0 24.027582 14-15 CCAACAC 100 0.0016454804 23.746822 3 ACGCAGA 1465 0.0 23.665775 7 TACATGG 605 0.0 23.550568 2 CGCAGAG 1470 0.0 23.272762 8 ACATGGG 595 0.0 22.349949 3 GCAGAGT 1525 0.0 22.121841 9 >>END_MODULE